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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for Recombinant Human ROR1 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Mouse IP6K1 was sub-cloned into SFB (S-protein/FLAG/SBP) - tagged destination vector using the Gateway cloning strategy (Invitrogen). Catalytically inactive IP6K1 (K226A ...
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bioRxiv - Biochemistry 2020Quote: MBP-tagged (pTHMT) full-length SARS-CoV2 nucleocapsid protein was expressed in Escherichia coli BL21 Star (DE3) cells (Life Technologies). Bacterial cultures were grown to an optical density of 0.7 to 0.9 before induction with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Synthetic Biology 2021Quote: ... Clarified lysates containing His6x-tagged proteins were subjected to gravity-flow Ni2+-affinity purification using HisPur Ni-NTA Resin (ThermoFisher) following manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... nucleotide sequences encoding N-terminal FLAG-tagged VACV proteins were subcloned between the BamHI and XbaI restriction sites of a pcDNA4/TO vector (Invitrogen). Alternatively ...
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bioRxiv - Molecular Biology 2020Quote: ... The Mex67-3Flag tagged protein was isolated using 200 µL of pre-washed magnetic Pan Mouse Dynabeads (Thermo Fisher Scientific) coupled to 34 µg of anti-Flag M2 mouse monoclonal antibody (Sigma) ...
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bioRxiv - Microbiology 2020Quote: Expression vectors for FixL and FixJ His6-tagged proteins were generated using the Gateway high-throughput recombinational cloning system (Invitrogen). The entire fixJ gene or fixL amino acids 329-851 (lacking transmembrane domains ...
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bioRxiv - Microbiology 2020Quote: ... The HA-tagged proteins were captured with the Pierce™ HA-Tag Magnetic IP/Co-IP Kit (Thermo Scientific™). Magnetic beads (0.25 mg ...
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bioRxiv - Molecular Biology 2020Quote: ... The entry clones were recombined into the Gateway binary vector pGWB552 tagged with G3 green fluorescent protein (G3GFP) using LR clonase (Invitrogen) (Nakagawa et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... the receptor Fc-tagged ectodomains present in the conditioned media were captured on protein A-coated 384-plates (Thermo Scientific), and stored at 4 °C until use ...
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bioRxiv - Cell Biology 2020Quote: Bacterial expression constructs coding for His6/SUMO tagged fusion proteins of rat SHANK3 were generated in pET-SUMO (Thermo Scientific) as described 14 ...
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bioRxiv - Molecular Biology 2021Quote: ... For Protein A-tagged strains, immunoprecipitation was carried out with Rabbit IgG-conjugated (MP-Biomedicals, SKU 085594) Dynabeads (Invitrogen, 14301) as described 57 ...
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bioRxiv - Microbiology 2020Quote: HEK293 were transfected with 3xFlag-tagged plasmids encoding each SARS-CoV-2 protein by Lipofectamine 3000 (Thermo Fisher Scientific, #L3000015). 3×107 HEK293 cells were harvested in 10 ml lysis buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Microbiology 2021Quote: ... concentrated eluates issued from immunopurification of endogenous and RACK1-HA-tagged protein are separated on 10% Tris-glycine SDS-PAGE gels (Invitrogen), and stained with Imperial Protein Stain (Thermo Fisher) ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged proteins were then eluted with room temperature Elution Buffer with 2.5 mg/mL 3XFLAG Peptide (Thermo Fisher # A36806) for 15 min at room temperature ...
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bioRxiv - Biochemistry 2022Quote: The MS/MS spectra were searched against the NCBI Mycobacterium smegmatis database with common lab contaminants and the sequence of the tagged bait proteins were added using SEQUEST within Proteome Discoverer 1.4 (Thermo Fisher). The search parameters were as follows ...
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bioRxiv - Plant Biology 2023Quote: ... and RAF39 driven by the cauliflower mosaic virus (CaMV) 35S promoter and C-terminally tagged with green fluorescent protein (GFP) was achieved using Gateway LR Clonase II (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... human protein database with common lab contaminants and the sequence of the tagged bait proteins added using SEQUEST within Proteome Discoverer 1.4 (Thermo Fisher). The search parameters were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... the GFP-tagged proteins were immunoprecipitated by incubating the extracts with 15 μl of anti-HA magnetic beads (Thermo Scientific) for 2 h at 4°C with gentle shaking ...
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bioRxiv - Cell Biology 2023Quote: Human ApoE protein was quantified using the Invitrogen Human ApoE ELISA following the manufacturers guidelines (Invitrogen. Cat no. EHAPOE).
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bioRxiv - Microbiology 2019Quote: ... The extracted protein was labeled with Click-iT™ O-GlcNAc Enzymatic Labeling System (Invitrogen, C33368) following with the manufacture’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Labeled phalloidins and secondary antibodies and the protein molecular weight marker were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The cleaved protein was labeled by introducing 500 nmole Alexa Fluor 488 maleimide (Thermo Fisher Scientific) to the cleaved solution and incubating at 4 °C overnight ...
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bioRxiv - Biophysics 2019Quote: ... The antibody was labeled by TexasRed (Texas Red™-X Protein Labeling Kit, ThermoFisher, CA, USA). Labeling efficiency of the antibody was determined as 1.52 TexasRed/antibody ...
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bioRxiv - Systems Biology 2021Quote: ... The protein digests were labeled with 10-plex Tandem Mass Tag (TMT) reagents (Thermo Fisher Scientific) (Dayon et al. ...
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bioRxiv - Biochemistry 2022Quote: CENP-SX was labeled using Alexa Fluor 647 Protein Labeling Kit (ThermoFisher Scientific, Waltham, US-MA) according to the manufacturer instructions.
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bioRxiv - Plant Biology 2020Quote: ... Labeled proteins were run on an 8-16% precise Tris-Glycine SDS page gel (ThermoFisher Scientific). Serine protease activity was visualized by in-gel fluorescence using the Typhoon 8600 variable mode Imager (Molecular Dynamics Inc ...
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bioRxiv - Cancer Biology 2022Quote: ... Gelatin was fluorescently labeled using Alexa Fluor 488 Protein labeling kit (Molecular Probes, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Gelatin was fluorescently labeled using Alexa Fluor 488 Protein labeling kit (Molecular Probes, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... galectin-8 was labeled by Alexa Fluor 568 Protein Labeling Kit (Molecular Probes, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... galectin-8 was labeled by Alexa Fluor 568 Protein Labeling Kit (Molecular Probes, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Labeled proteins were bound to streptavidin-agarose beads (170 μL resuspended beads per sample, Thermo Fisher) while rotating overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Purified CLASP1 was labeled using Alexa Fluor 488 Microscale Protein Labeling Kit (ThermoFisher Scientific, cat. #A30006) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... Protein was labeled by incubating with 0.8 molar equivalents of Alexa Flour 488 maleimide (Invitrogen A10254). Proteins were buffer exchanged into buffer C and concentrated to less than 50 mM (to avoid aggregation).
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bioRxiv - Microbiology 2024Quote: ... Translated proteins labeled with [35S] Met were resolved in a 4%-12% SDS-PAGE gel (Invitrogen), dried onto a Whatman filter paper ...
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bioRxiv - Biophysics 2024Quote: ... Antibodies (100 µg) were labeled with Alexa Fluor® 594 Protein Labeling Kit (Thermo-Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... CD45-APC 1:200, Biolegend, CD117-APC 1:100, Biolegend; CD140a-APC 1:100, Thermo Fisher; CD29-APCe780, 1:250, Thermo Fisher, Biolegend) was prepared in the FACS buffer with 100 ng/ml DAPI ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were washed and 50μl Pierce recombinant protein A/G with peroxidase (Thermo Fisher catalog no: 32490) added at 1:10,000 in PBS-T with 1% milk as a secondary and incubated 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: Microscopic images of the recombinant proteins in chambered cover glass (Thermo Fisher Scientific, Nunc Lab-Tek II) were taken using a confocal microscope (Olympus ...
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bioRxiv - Molecular Biology 2020Quote: Microscopic images of the recombinant proteins in chambered cover glass (Thermo Fisher Scientific, Nunc Lab-Tek II) were taken using a confocal microscope (Olympus ...
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bioRxiv - Biochemistry 2020Quote: ... Concentration of the recombinant peptides was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, USA) with bovine serum albumin as the standard.
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bioRxiv - Plant Biology 2021Quote: For recombinant protein expression in Pichia pastoris GS115 (Multi-Copy Pichia Expression Kit Instructions, Thermo Fisher Scientific), constructs of IF1 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant forms of SyAM in culture supernatant were isolated by protein A affinity resins (MabCapture, ThermoFisher Scientific) using Pierce gentle Ag/Ab elution buffer (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... previously cultured for 24h with IL-15/IL-15R complex recombinant protein (100ng/ml, Thermo Fisher Scientific) and retinoic acid (100ng/ml) ...
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bioRxiv - Immunology 2022Quote: Recombinant gH/gL proteins were biotinylated using the EZ-Link NHS-PEG4-Biotin Kit (ThermoFisher, catalog #21330) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: The recombinant proteins were purified using nickel sepharose beads (Ni-NTA, Thermo Fisher Scientific, Waltham, MA, USA). The lysate supernatant from the 50 ml culture was mixed with 300 μl Ni-NTA beads and incubated overnight at 4° in the lysis buffer supplemented with 5 mM imidazole ...
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bioRxiv - Immunology 2022Quote: Recombinant tri-S protein was heat-denatured at 100°C for 3 min in loading buffer (Invitrogen) containing 1X sample reducing agent (Invitrogen) ...
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bioRxiv - Pathology 2020Quote: ... A 1ug/mL mixture of 50μL per well containing recombinant protein in carbonate buffer (0.1M Na2CO3 0.1M NaHCO3 pH 9.3) was used to coat MaxiSorp (ThermoFisher) 96-well plates overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Endotoxin was removed from recombinant protein using High Capacity Endotoxin Removal Spin Columns (ThermoFisher Scientific Catalog #88274) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Recombinant proteins and antibody 47D11 were expressed transiently in FreeStyle™ 293-F Cells (Thermo Fisher Scientific) and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: All recombinant proteins were expressed and purified using a mammalian expression system via Expi293F cells (Life Technologies), as described in detail earlier (30 ...