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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for NEFH Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: Recombinant bacmid production was carried out under manufacturer’s instructions (Bac-to-bac system, Life Technologies). Competent E ...
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bioRxiv - Developmental Biology 2024Quote: Recombinant FGF10 protein was labeled using an Alexa Fluor 488 Microscale Protein Labeling Kit (Invitrogen). To reduce the loss of physiological activity of FGF10 ...
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bioRxiv - Cell Biology 2023Quote: ... the recombinant adenovirus extract was injected into Slide-A-Lyzer Dialysis Cassettes (Thermo Fisher Scientific) and dialyzed in a dialysis buffer at 4 °C with stirring ...
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bioRxiv - Microbiology 2024Quote: Recombinant spike proteins were biotinylated with EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, Cat. # A39259) for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher ...
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bioRxiv - Genomics 2024Quote: ... 50 µL of 20 mg/mL recombinant Proteinase K solution (Life Technologies, Catalogue number: AM2548) was then added ...
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bioRxiv - Microbiology 2024Quote: ... equi were prepared using a recombinant plasmid from the TA cloning® kit (Invitrogen, USA). A 10-fold serial dilution of the plasmid (from an initial concentration of 0.5x108 copy number/μL ...
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bioRxiv - Immunology 2024Quote: The recombinant proteins were purified using a CaptureSelectTM C-tagXL Affinity Matrix prepacked column (ThermoFisher), followed the manufacturer’s guidelines as reported previously (25 ...
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bioRxiv - Immunology 2024Quote: ... and recombinant human interleukin 4 (rhIL-4) (300 U/mL; Thermo Fisher Scientific, Waltham, MA). On day 2 and day 5 post-monocyte isolation ...
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bioRxiv - Cell Biology 2024Quote: ... in presence of 40 units of RNaseOUTTM Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific #10777-019) and 200 units of Reverse Transcriptase per 20 µL of reaction mixture ...
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bioRxiv - Genetics 2023Quote: ... goat α rabbit Cy3 or donkey α rabbit Alexa488 (ThermoFisher). Neuronal membranes were visualized with 1:100 goat α HRP-Alexa 647 (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-rabbit secondary antibody (Thermofisher anti-rabbit IgG AF633). Images were acquired using the Thunder Imager Tissue (Leica ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit-antiespin (Invitrogen PA5-55941 ...
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bioRxiv - Neuroscience 2020Quote: ... calretinculin (rabbit, ThermoFisher), SARS-CoV/SARS-CoV-2 Spike S2 (mouse ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal (Invitrogen) immunofluorescence 1/100 ...
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bioRxiv - Neuroscience 2019Quote: ... GST(rabbit, Invitrogen), GFP (rabbit ...
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bioRxiv - Neuroscience 2019Quote: ... GFP (rabbit, Invitrogen), GluN2A(rabbit ...
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bioRxiv - Cell Biology 2023Quote: α-rabbit (Invitrogen, A10042), donkey α-mouse (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit-Cxcr4 (Thermofisher), and rat anti-somatostatin (Millipore) ...
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bioRxiv - Cell Biology 2019Quote: A mAb producing CHOK1 cell line (CHOK1-mAb) was seeded at a density of 2 × 105 cells/ml in SFM-II media (Gibco, 12052098) in a Kuhner orbital shaker (170rpm ...
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bioRxiv - Immunology 2020Quote: ... Reactivity of the CIS43 mAb for the CIS43 VLPs was determined by measuring optical density at 450□nm (OD450) using an AccuSkan platereader (Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... and subsequently stained for 1 h at room temperature with anti-NP mAb 1C7 antibody labeled with an Alexa488-fluorescent marker using the Alexa Fluor™ 488 Antibody Labeling Kit (Invitrogen). Immunofluorescence-labelled cells were washed twice in PBS-EDTA after incubation with the antibody and then subjected to flow-cytometry analysis using a Gallios flow cytometer (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-ELAV (MAb 7D, 1:20, which recognizes 7 amino acids unique to ELAV) and anti-GFP (1:250; Invitrogen A11122) and visualized with Alexa Fluor 488- and/or Alexa Fluor 647-coupled secondary antibodies (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... washed three times in HBSS/1% BSA and incubated at 4 °C for 15 minutes in the dark with the following antibodies: LDLR (1/100; MAB CL 472413; Fisher scientific), CD36 (1/10 ...
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bioRxiv - Immunology 2022Quote: ... Fc and non-digested mAbs were removed from the flow-through by a 2 h incubation at RT with 200 µl of protein A resin per mg of initial mAb (Thermo Scientific). Finally ...
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bioRxiv - Microbiology 2022Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 μL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Immunology 2019Quote: ... for the HC and LC of each mAb was transfected into 5ml of suspension Expi293 cells according to the manufacturer’s instructions (Thermo Fisher Scientific) in 50ml tubes ...
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bioRxiv - Biochemistry 2020Quote: ... primary CD4+ T cells were isolated from cones from anonymous donors with approval from the National Health Service Blood and Transplant (NHSBT) and stimulated with CD3 and CD28 mAbs on Dynabeads (Thermo Fisher) in the presence of 100 U/ml IL-2 to produce T cell blasts ...
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bioRxiv - Immunology 2020Quote: ... followed by staining with mAbs MM1a (anti-CD3) and ILA105 (anti-CD8) and secondary antibodies and Fixable Yellow Dead Cell Stain (Thermofisher Scientific). These cells were sorted using a FACSAria III (BD) ...
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bioRxiv - Immunology 2020Quote: ... the 5D5Δg Fab was produced by site-directed mutagenesis of the mAb 5D5 VH region using Accuprime Pfx Supermix (Thermo Fisher Scientific). 5D5Δg Fab was expressed in HEK293F cells and purified by chromatography as described above.
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Cryptococcus neoformans secretes small molecules that inhibit IL-1β inflammasome-dependent secretionbioRxiv - Immunology 2019Quote: ... cells were blocked for one h with a 10% FBS solution and stained for GXM with mAb 18B7 (10µg/mL) as a primary Ab and “Alexa Fluor® 633 Goat Anti-Mouse IgG” (Life Technologies) as a secondary Ab ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies (anti-mouse nsP2 and E2 mAbs) were then added for 1h followed by incubation with Alexa Fluor (AF) 488-conjugated chicken anti-mouse antibody (Invitrogen, USA). Approximately 1×104 cells were acquired by the LSR-Fortessa flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Microbiology 2020Quote: ... Cells were immunolabelled for 1 hour at RT with the indicated murine mAb and 45 min with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Life Technologies). Immunolabelled coverslips were counterstained with DAPI (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... for 20 min before incubation overnight at 4°C in PBS-BR containing any of the following mAbs or reagents: DAPI nucleic acid (Molecular Probes), phalloidin Alexa fluor 488 (Molecular Probes) ...
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bioRxiv - Immunology 2023Quote: The 14.4.4 mAb producing hybridoma cell line (isotype: mouse IgG2a, kappa) was maintained at 37 °C and 5% CO2 in RPMI (Life technologies) supplemented with 5% FCS (Biowest) ...
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bioRxiv - Immunology 2023Quote: ... The 14.4.4 mAb and the I-Ak-reactive 11-5.2 mAb were randomly labeled via available lysine residues with Alexa Fluor 647 carboxylic acid succinimidyl ester (Thermo Fisher Scientific) according to the manufacturer’s instructions and purified via gel filtration (Superdex-200 10/300 GL ...
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bioRxiv - Biochemistry 2023Quote: ... The anti-F or G mAbs were prepared under serum-free conditions using HyClone SFM4MAb (Thermo Fisher Scientific Inc., Rockford, IL) and purified by protein G-Sepharose affinity chromatography.
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bioRxiv - Microbiology 2023Quote: Splenocytes were stimulated with the peptide pools described in the memory phenotypic assay and supplemented with anti-CD28 and anti-CD49d mAbs (ThermoFisher Scientific) both at 1.0 μg/ml or a tissue culture medium with 1% DMSO as a negative control ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Immunology 2023Quote: ... T cells were distributed into the round bottom 96 well plates at 3000 cells per well and stimulated by CD3/CD28 mAb-coated beads (ThermoFisher Scientific) at a ratio of 10:1 ...
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bioRxiv - Immunology 2023Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 µL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Genomics 2023Quote: A mAb producing CHOK1 cell line (CHO K1 mAb) was seeded at a density of 2 × 105 cells/ml in 50ml SFM-II media (Gibco, 12052098) in 8 replicate shake flasks in a Kuhner orbital shaker at 170rpm at 5% CO2 ...
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bioRxiv - Immunology 2023Quote: Cryopreserved/thawed PBMCs were stained for 30 minutes at 4°C with the following reagents or conjugated anti-human mAbs: Live/Dead Aqua (Life Technologies), anti-CD4-PerCP (L200 ...
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bioRxiv - Microbiology 2023Quote: ... berghei infected HepG2 cells were immunolabeled using anti-PbHSP70 (1:200, 2E6 mouse mAb) (62)to mark EEFs followed by donkey anti-mouse Alexafluor488 (Invitrogen A21202). MSP1 was immunolabeled with Anti-PBANKA_083100 ...
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bioRxiv - Microbiology 2024Quote: ... Polprim and RPA -specific mAbs (approximately 100 µg of each ligand) were separately bound to 5mg of M-280 Tosyl activated Dynabeads (Invitrogen # 14203) O/N at 37⁰C on a rotating wheel ...
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bioRxiv - Pathology 2024Quote: The binding affinity of Gremlin-1 or Gremlin-1-mAb mixtures to fluorescein-labelled Heparin (Heparin-FL, H7482, Thermo Fisher Scientific) was measured in a fluorescence polarization assay ...
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bioRxiv - Microbiology 2020Quote: ... All PCR reactions were carried out using Recombinant Taq DNA Polymerase (5U/μl from Thermo Scientific) and standard PCR cycling conditions.
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bioRxiv - Cell Biology 2022Quote: ... Purified recombinant plasmid DNA was digested with PacI and transfected into HEK293 cells with Lipofectamine (Invitrogen) according to manufacturer’s instructions ...