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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for 4' Bromo 3' fluoro 2 morpholinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Functional and epitope specific monoclonal antibody discovery directly from immune sera using cryoEMbioRxiv - Immunology 2024Quote: ... Cells were resuspended in phosphate buffered saline(PBS) + 2% fetal bovine serum(FBS) 200 ng/mL 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific). Cell sorting was performed on a BD FACSAria Fusion (BD Biosciences).
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bioRxiv - Bioengineering 2024Quote: ... for 2 h and a 1 µg mL−1 solution of 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific 62248) for 30 min ...
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bioRxiv - Genomics 2020Quote: ... The cells were passaged every 2-3 days using 0.25% Trypsin-EDTA (1X) (Gibco, Cat# 25200056) as the dissociation buffer.
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bioRxiv - Microbiology 2020Quote: ... Poly (I:C) and 2’-3’ cGAMP were delivered into cells using Lipofectamine 2000 (Thermo Fisher, USA) as described previously.
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bioRxiv - Biochemistry 2021Quote: ... followed by 2 weeks of selection with 3 μg mL−1 of puromycin (Thermo Fisher Scientific). Then ...
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bioRxiv - Genomics 2021Quote: ... Samples were treated with 2–3 drops of Image-iT FX Signal Enhancer (Molecular Probes; 136933) for 15◻min with rotation at room temperature ...
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bioRxiv - Physiology 2021Quote: 2-3 viable human slices were incubated with Fluo4-AM (6 μM, Invitrogen cat. No. F1221) for 1h in 3 mM HEPES buffer (125 mmol/l NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... SNLP mouse fibroblasts and passaged upon ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher) at room temperature.
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bioRxiv - Cell Biology 2023Quote: ... All cells were passaged every 2-3 days using 0.05% Trypsin/EDTA (Gibco, Catalog no. 25300054). All cell lines were incubated at 37°C and maintained at 5% CO2.
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bioRxiv - Molecular Biology 2023Quote: ... fed daily with growth factors and passaged every 2-3 days using TrypLE Express enzyme (GIBCO) to detach cells and trypsin inhibitor (GIBCO ...
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bioRxiv - Neuroscience 2023Quote: ... 11319718) were coated dropwise with 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine Perchlorate (DiI, 2 mg, Invitrogen, D282) dissolved in dichloromethane (400 ul ...
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bioRxiv - Developmental Biology 2024Quote: ... Cell passaging was performed every 2–3 days using 0.25% Trypsin-EDTA (1X) (Gibco, Cat# 25200056) as the dissociation buffer.
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bioRxiv - Genetics 2024Quote: Testes from 2–3 day-old flies were dissected and transferred to Schneider’s insect medium (Gibco) with or without 50 ng/µl α-amanitin ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were prepared by incubating with a 2% solution of (3-aminopropyl)trimethyoxysilane (313255000, Acros Organics) diluted in isopropanol ...
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bioRxiv - Immunology 2023Quote: ... Peptides were trapped on precolumn (PepMap100 C18 3 μm; 75 μm × 2 cm; Thermo Fisher Scientific) and separated on an EASY-Spray column (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... 3 and 24 hours later the plating medium was exchanged against NeurobasalTM containing 2% BL27 (Gibco) and 0,8 mM L-glutamine (all Gibco) ...
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bioRxiv - Systems Biology 2022Quote: ... Peptides were trapped on precolumn (PepMap100 C18 3 μm; 75 μm × 2 cm; Thermo Fisher Scientific) and separated on an EASY-Spray column (ES903 ...
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bioRxiv - Immunology 2023Quote: ... The tissue was then cut into 2-3-cm segments and incubated in RPMI media (ThermoFisher) containing 5 mM EDTA ...
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bioRxiv - Physiology 2024Quote: ... 3-month and 2-year tissue samples were quantified using a BCA Assay (Thermo Scientific VLBL00GD2). Equal amounts of proteins were run on 4%–20% Tris-glycine gels (Invitrogen WXP42012BOX) ...
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bioRxiv - Immunology 2024Quote: ... cells were diluted 1:3 in PBS with 2 mM EDTA and 1:1000 DAPI (ThermoFisher). Cells were counted using Forward Scatter (FSC ...
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bioRxiv - Immunology 2024Quote: ... for 3 hours followed by one-hour treatment of 2 mM ATP (Thermo Fisher Scientific, R0441). To determine how efferocytosis affect caspase-1 cleavage ...
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bioRxiv - Bioengineering 2024Quote: ... and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT, 0.5mg/ml, #V13154, Thermo Fisher) for 1 h ...
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bioRxiv - Cell Biology 2024Quote: Sodium salt of 3-methyl-2-oxobutanoic acid (KIV; cat # AC189720050) was purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 days later cells were detached using 3 ml of TrypLE (Thermo Fisher, CAT: 12604021). Cells were moved to a non-tissue culture treated 6-well plate (VWR ...
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bioRxiv - Plant Biology 2020Quote: ... on 4-16% (Figures 4A and 7) or 3-12% (Figures 4C and 6) NativePAGE gels (Life technologies). Cathode Running buffer (Life technologies ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in mouse anti-Hu primary antibody at 4°C (1:200 in 3% block; Invitrogen). After three 5-min PB rinses at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... CHIKV was labeled with the lipophilic fluorescent probe DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt; Life Technologies), as described previously [5] ...
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bioRxiv - Neuroscience 2020Quote: ... 4) counterstained with the nuclear dye TO-PRO-3 (diluted 1:10’000; Life Technologies, Inc., Gaithersburg, MD, USA). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE was performed using NuPAGE 4-12% Bis-Tris and 3-8% Tris-Acetate gels (Life Technologies). Nitrocellulose membranes were developed with horseradish peroxidase (HRP ...
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bioRxiv - Genetics 2020Quote: mRNA was collected from groups of 10 whole larvae (n=3–4 replicates per line) using Trizol (ThermoFisher) and reverse transcribed to cDNA using SuperScript III Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... and Antibiotic-Antimycotic Mixed Stock Solution (Nacalai) and were passaged every 3-4 days with TryPLE (ThermoFisher Scientific). For microscopy observations ...
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bioRxiv - Bioengineering 2020Quote: ... Bodipy FL c16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid, Thermofisher) was diluted in sterile RPMI-1640 (Corning ...
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bioRxiv - Molecular Biology 2020Quote: ... a sample of eluates (3%) was separated on acrylamide NuPAGE Novex 4–12% Bis-Tris gels (Life Technologies) and analyzed by silver staining.
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bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-4 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
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bioRxiv - Microbiology 2021Quote: ... Paris) and pCMV-VSV-G at a ratio of 4:3:1 with Lipofectamine 3,000 (Thermo Fisher Scientific). Supernatants were collected 48 h after transfection ...
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bioRxiv - Physiology 2022Quote: ... The samples were combined with 1 ml of 3:13 dilution of Erlich’s reagent [1.5 g of 4- (dimethylamino) benzaldehyde (ThermoFisher); 5 ml ethanol ...
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bioRxiv - Cancer Biology 2024Quote: ... at 95⁰C for 3 min and loaded onto a SDS-polyacrylamide gel (4-12%, Invitrogen NuPAGE, #NP0336BOX). After size separation ...
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bioRxiv - Biochemistry 2023Quote: ... the filtered supernatant was loaded onto 3-4 mL immobilized D-galactose gel (Pierce™, Thermo Scientific™) equilibrated by gravity flow with Gal A buffer (50 mM Na-phosphate pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... and then analysed on Novex WedgeWell 4-12 % Tris-Glycine Gels or 3-8 % Tris-Acetate gels (Invitrogen). Tris-Glycine gels were run at 180 V for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: 50 000 enriched CD11b+ LCs were seeded and incubated for 10min at 37°C with 2mM Bodipy-C11 (581/591) (4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid; Invitrogen) in PBS ...
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bioRxiv - Genomics 2024Quote: ... The remaining cells are resuspended in our working cell buffer solution (AMES/0.4% BSA + 3 µM ActD) and incubated with CD90.2/Thy1.2 antibody conjugated to APC (#17-0902-83; Invitrogen, Carlsbad ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were cultured for 3-4 days in phenol red-free DMEM/F-12 (Thermo Fisher 11039021) supplemented with 5% charcoal-dextran-treated FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Mitochondrial proteins were solubilized with 1% digitonin resolved on 4-16% or 3-12% NativePAGE Novex gels (Invitrogen). For blotting ...
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bioRxiv - Molecular Biology 2024Quote: ... protein samples were resolved by SDS-PAGE on pre-cast NuPAGE™ gels (1.0 mm 4–12% Bis-Tris or 1.5 mm 3–8% Tris-Acetate for HMW TRIM37, Invitrogen) with molecular weight ladders (PageRuler Plus or HiMark pre-stained protein standard for HMW TRIM37 ...
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bioRxiv - Cell Biology 2022Quote: ... metaphase II-arrested eggs were microinjected with 2-3 picolitres of Rec8 antiserum (13) (1:2 dilution) and Alexa Fluor 488 Dextran 10,000 MW (Molecular Probes, D22910; 1:40 dilution) in 0.05% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampal neurons 13-15 days-in-vitro (DIV) were transfected with plasmid (2 h, 2-3 µg per dish) with Lipofectamine 2000 (Invitrogen, #52887, minimum 24 h). For pH imaging experiments ...
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bioRxiv - Neuroscience 2020Quote: ... stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Grand Island, NY, USA) for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... Each antibody (2-4 µg per reaction) and IgG control were incubated with Dynabeads (Invitrogen) for at least 4 h at 4C with rotation ...
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bioRxiv - Neuroscience 2021Quote: ... fixed larvae were stained with 2.5 ug/ml 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) diluted in PBS to label all cell nuclei ...
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bioRxiv - Immunology 2022Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) and LysoTracker Red DND-99 were purchased from Invitrogen, Carlsbad ...