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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for 3 Ethyl 3 methyloxolane 2 5 dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 3 mM DTT) and homogenized using a Dounce homogenizer followed by DNaseІ treatment (3 U/µl, ThermoFisher) for one hour on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... UM-UC-3 cells were fluorescently labeled using 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI, ThermoFisher, #D3899) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... sulfosuccinimidyl-2-[biotinamido]ethyl-1,3-dithiopropionate) (sulfo-NHS-SS-biotin) and streptavidin agarose were from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and surface proteins were biotinylated with 0.5 mg/mL sulfosuccinimidyl-2-(biotinamido) ethyl-1,3-dithiopropionate (ThermoFisher) on ice for 45 minutes with gentle shaking ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg/ml L-(tosylamido-2-phenyl ethyl) chloromethyl ketone (TPCK)-treated trypsin (Thermo Scientific Pierce), and 1x antibiotic-antimycotic (Corning) ...
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bioRxiv - Immunology 2023Quote: ... and 0.1% L-(tosylamindo-2-phenyl) ethyl chloromethyl ketone (TPCK)-treated trypsin (Affymetrix, Cleveland, OH, USA). Lastly ...
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bioRxiv - Biochemistry 2024Quote: ... The modified nucleosomes were then conjugated with PEAS [N-((2-pyridyldithio)ethyl)-4- azidosalicylamide] (Thermo Fisher), iodinated with [125I] and bound to saturating concentrations of WT or mutant Arp5 complexes ...
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bioRxiv - Biophysics 2024Quote: ... 1 μg/mL L-(tosylamido-2-phenyl ethyl) chloromethyl ketone (TPCK)-treated trypsin (Thermo Scientific Pierce), and 1× antibiotic-antimycotic (Corning) ...
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bioRxiv - Immunology 2024Quote: ... 1 µg/mL L-(tosylamido-2-phenyl ethyl) chloromethyl ketone (TPCK)-treated trypsin (Thermo Scientific Pierce), and 1× antibiotic-antimycotic60,61 ...
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bioRxiv - Molecular Biology 2021Quote: ... oligonucleotides from homologous regions D-ORF5 5′CCGctgagCAAGGTAGCCTCGTCTATTGGAC 3′ and R-ORF7 5′CCGctcgagTTCTTCATCTTCAATATTATGTC3′ were used using the PCR Reagent System Kit (Invitrogen, Life Technologies Inc.). The reaction mixture was prepared with l00 ng of recombinant TnGV DNA ...
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bioRxiv - Biophysics 2021Quote: ... Protein samples (12 μL) were mixed with 3 μL 5 M DTT and 5 μL 4x NuPAGE™ LDS sample buffer (Thermo Fisher Scientific) and incubated (95 °C ...
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bioRxiv - Biophysics 2023Quote: ... The grids were blotted for 3.5 s with 3 force after waiting for 5 s and immersed in liquid ethane using Vitrobot (Mark IV, Thermo Fisher Scientific/FEI) in condition of 100% humidity and 8°C.
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bioRxiv - Biophysics 2023Quote: ... The grids were blotted for 5 s with 3 force after waiting for 5 s and immersed in liquid ethane using Vitrobot (Mark IV, Thermo Fisher Scientific/FEI) in condition of 100% humidity and 8°C.
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bioRxiv - Neuroscience 2020Quote: ... Some slices (used for experiments in Fig. 2 and 3) were cultured in media that included 2 % B-27 Supplement (Thermo Fisher Scientific). Slices were maintained at 37°C and 5 % CO2 and used for experiments at DIV10-15.
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bioRxiv - Neuroscience 2024Quote: ... followed by culturing with the neural induction medium supplemented with 20 ng/ml human FGF-2).61 The passage was performed twice per week (1:2 or 1:3) using Accutase (Cat #A1110501, Thermo Fisher Scientific) by vigorously breaking pellets to remove neuronal cells ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 5 mM DTT, 50 mM HEPES, 3 mM MgCl2, 2 mM PMSF, 1 Pierce™ protease inhibitor mini tablet/2 L; Thermo Scientific). The lysate was sonicated then incubated with 500 units/1 L culture Benzonase Nuclease (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated for 2-3 hours at room temperature with 2 µg/ml donkey anti-goat Alexa Fluor 488 (Cat# A21206, Thermo Fisher Scientific), 2 µg/ml donkey anti-rabbit Alexa Fluor 568 (Cat# A10037 ...
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bioRxiv - Immunology 2021Quote: ... Samples from Biosafety Level 3 were inactivated for 2 hours at RT with 4% paraformaldehyde (ThermoFisher Scientific) after extracellular and intracellular staining.
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bioRxiv - Developmental Biology 2021Quote: ... Cells were passaged 1:10 every 2–3 days with 1× trypsin-EDTA 0.25% (Gibco 25200-056).
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... The suspension of pellet 2+3 was filtered through 40-μm cell strainer (Fisher scientific, Cat # 22363547), followed by final wash in complete Ham’s F10 medium ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were loaded onto a C18 trap column (3 μm, 75 μm × 2 cm, Thermo Fisher Scientific) connected in-line to a C18 analytical column (2 μm ...
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bioRxiv - Biochemistry 2022Quote: ... and after immobilization of HER2- Nb and HER2-Nb-GEPII 1.0 was assessed using 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) reagent (ThermoFisher). After immobilization of HER2-Nb and HER2-Nb-GEPII 1.0 on the cell surface ...
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bioRxiv - Neuroscience 2022Quote: BDA was visualized with fluorophore-conjugated streptavidin (Thermo Fisher Scientific, Table 2; 1:1,000 for 3 h). The reaction was enhanced using the biotinylated tyramine (BT)-glucose oxidase (GO ...
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bioRxiv - Microbiology 2022Quote: ... All cells were passaged 2-3 times per week with 0.25% Trypsin/0.02% EDTA (Gibco Cat#25200056). Cells used in all experimental set-ups were between passage 5 and 30.
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bioRxiv - Cell Biology 2022Quote: ... All the LUVs contained 2 mol% of 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (DHPE)-tRed (Molecular Probes). Lipids were mixed in chloroform ...
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bioRxiv - Cancer Biology 2021Quote: ... antagomir 2 from Exiqon (miRCURY LNA microRNA Power Inhibitor; 4100464-002) and antagomir 3 from Thermo Scientific Dharmacon (miRIDIAN hairpin inhibitor ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were injected into a trap column (nanoViper C18, 3 μm, 75 μm × 2 cm, Thermo Scientific) with 12 μL of solvent A (0.1% formic acid ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid DNA (2-3 μg) was diluted in 400 μL Opti-MEM (11058021, Thermo Fisher Scientific, USA) spiked with 2-3 μL Plus reagent (15338100 ...
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bioRxiv - Genomics 2020Quote: ... the skin samples were washed 2-3 times with buffer saline (1X PBS; Gibco, Thermo Fisher Scientific). The adipose tissue and associated blood vessels were removed ...
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bioRxiv - Genomics 2020Quote: ... the skin samples were washed 2-3 times with buffer saline (1X PBS; Gibco, Thermo Fisher Scientific). The adipose tissue and associated blood vessels were removed ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were routinely passaged after 2-3 days or after reaching ∼80 % confluency using TrypLE Express (Gibco) for the detachment of cells from culture flasks ...
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bioRxiv - Immunology 2022Quote: ... 2 μm paraffin-embedded kidney sections were incubated with an anti-cleaved caspase-3 antibody (ThermoFisher Scientific). After incubation with goat anti-rabbit-HRP secondary antibody (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... in the reverse phase using a preconcentration column (75 μm DI × 2 cm, 3 μm, Thermo Scientific) and an analytical column (Acclaim PepMap C18 ...
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bioRxiv - Genetics 2022Quote: ... Cells were passaged at 70-80% confluence every 2-3 days using Trypsin 0.25% EDTA (Life Technologies). mESCs were plated at a density of 1 x 105 cells/ml ...
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bioRxiv - Microbiology 2022Quote: PBMCs were thawed according to standard procedure and rested 2-3 h in IMDM (Gibco/ThermoFisher Scientific) containing 5% human AB serum (SAB ...
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bioRxiv - Microbiology 2022Quote: PBMCs were thawed according to standard procedure and rested 2-3 h in IMDM (Gibco/ThermoFisher Scientific) containing 5% human AB serum (SAB ...
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bioRxiv - Microbiology 2022Quote: Anti-ORF8 mAb (#1-3-2) was coupled to aldehyde/sulfate latex beads (Thermo Fisher Scientific A37304). The antibody was mixed with the latex beads and shaken at room temperature overnight ...
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bioRxiv - Microbiology 2022Quote: ... adding 3 mL HPLC grade methanol and 2 mL HPLC grade chloroform (C297-4, Thermo Fisher Scientific), then shaking at 22°C for 1 hr ...
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bioRxiv - Genomics 2023Quote: ... and CRISPRmap library plasmid (2:3:4 ratio by mass) using Lipofectamine 3000 (Thermo Fisher Scientific L3000001) in lentiviral packaging medium (Opti-MEM (Thermo Fisher Scientific 31-985-070 ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated for 2–3 minutes (mins) at room temperature in 0.05% (v/v) trypsin (Gibco 25300-054), and tapped to release ...
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bioRxiv - Molecular Biology 2023Quote: U2OS 2-6-3 cells (Shanbhag et al., 2010) were cultured in McCoy’s 5A (Modified) Medium (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... DAPI (4’,6-Diamidino-2-henylindole, dihydrochloride) was obtained from Invitrogen (Catalog: D1306, CAS:28718-90-3).
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bioRxiv - Molecular Biology 2023Quote: ... An Acclaim PepMap 100 trap column (75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) was used together with an analytical PepMap RSLC C18 column (150 μm × 15 cm ...
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bioRxiv - Cell Biology 2023Quote: ... after the first fast centrifugation step platelets were incubated with 3 µM Fura-2 AM (#F1221, Invitrogen) and 0.2% Pluronic F-127 (#P3000MP ...
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bioRxiv - Biochemistry 2023Quote: ... cells were incubated with 1.2mM 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT, Thermo Fisher Scientific) solution ...
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bioRxiv - Systems Biology 2023Quote: Total RNAs (2–3 μg) were extracted from tobacco leaves using PureLink Plant RNA Reagent (Invitrogen, #12322012) and used for cDNA synthesis with M-MLV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was collected on day 3 and analyzed by human IL-2 ELISA Kit (Thermo Fisher) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2024Quote: ... A 2-3 cm2 skin biopsy harvested and transferred into NMR fibroblast medium: alphaMEM (Gibco™ 12571063) containing 15% FBS (Cytiva ...
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bioRxiv - Cancer Biology 2024Quote: Proliferation assays were performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Thermo Fisher Scientific) assay ...
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bioRxiv - Immunology 2024Quote: Jurkat cells were loaded with 3 µM Fura-2 AM and 0.05% Pluronic®F-127 (Invitrogen) in imaging buffer ...