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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for rno mir 137 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... 1:10 cDNA dilution was used for each reaction of quantitative RT-PCR analysis performed on an ABI Prism HT 7900 real-time PCR detection system (Applied Biosystems) equipped with SDS software version 2.4 (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... miR-200a-3p (MC10991) and negative control miRNA (Ambion) were used for these transfections.
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bioRxiv - Cell Biology 2021Quote: ... Relative gene expression was determined by quantitative real time PCR (q-RT-PCR) using the ViiA 7 Real Time PCR system (Applied Biosystems, USA). The reaction mixture consisted of 0.75μL 500nM forward primer ...
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bioRxiv - Biochemistry 2019Quote: ... Real-time PCR was performed on a Step-One Plus Real-Time PCR Detection System (Applied Biosystems, Waltham, MA, USA) using 2X Power SYBR Green Mastermix (ABI ...
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bioRxiv - Zoology 2023Quote: ... Quantitative real-time PCR was performed on a CFX Connect Real-Time PCR Detection System (Thermo Fisher Scientific., MA, USA) using an SYBR Green PCR kit (TransGen Biotech ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR was performed using the StepOnePlus Real-Time PCR detection system (Applied Biosystems, Foster City, CA, USA) and Platinum SYBR Green qPCR super Mix (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... The Real-time RT-PCR assays were conducted using TagMan One-Step RT-PCR kit (Thermo Fisher Scientific, Waltham, MA, USA) on Real-Time PCR System (Biorad ...
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bioRxiv - Microbiology 2020Quote: ... and ICV using real-time RT-PCR on a 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA) using previously reported primer and probe sets (Supplemental Table A ...
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bioRxiv - Cancer Biology 2020Quote: Real-time PCR reactions (RT-qPCR) were performed in the Step One Plus Real-time PCR System (Thermo Fisher Scientific). Taqman primers for PRDM1 ...
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bioRxiv - Molecular Biology 2021Quote: ... hsa-miR-25-3p or cel-miR-67 as control (25mM) using Oligofectamine (Invitrogen). Firefly and Renilla luciferase activities were measured using the Dual Luciferase Reporter Assay System (Promega) ...
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bioRxiv - Bioengineering 2023Quote: ... and primers specific for mmu-miR-147-3p (alias, hsa-miR-147b; Applied Biosystems). The relative abundance of EV miRNA was determined by TaqMan PCR using TaqMan Universal PCR Master Mix II and TaqMan primers and probes from Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: ... miRNA mimics and the miR-100 isoforms miR-100-5p and miR-100-3p (all from Life Technologies) using 15 pmol PepMute reagent (SignaGen Labs ...
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bioRxiv - Microbiology 2022Quote: ... Real time RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies) kit on a QuantStudioTM 7 Flex Real-Time PCR System with the primers specific for SARS-CoV-2 E gene (35) ...
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bioRxiv - Cell Biology 2021Quote: ... RT-PCR was performed on a 7500 Real-Time PCR System (Applied Biosystems) using the iTaq Universal SYBR Green Supermix (BioRad) ...
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bioRxiv - Physiology 2021Quote: ... Real-time PCR (RT-PCR) was performed on a Quantstudio 3 (ThermoFisher Scientific) using Taqman Fast Advanced Master mix (ThermoFisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... Real-time PCR (RT-PCR) was performed on a Quantstudio 3 (ThermoFisher Scientific) using GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Physiology 2023Quote: ... Real-time PCR (RT-PCR) was performed on a Quantstudio 3 (ThermoFisher Scientific) using Luna Universal qPCR master mix for Sybr Green primers (New England Biolabs ...
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bioRxiv - Physiology 2023Quote: ... Real-time PCR (RT-PCR) was performed on a Quantstudio 3 (ThermoFisher Scientific) using either Luna Universal qPCR master mix for Sybr Green primers (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... RT-Quantitative PCR (RT-qPCR) was performed on a 7900HT Fast Real-Time PCR System (Applied Biosystems) with Fast SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Immunology 2022Quote: ... Quantitative real-time RT-PCR was performed using the SYBR Green Master mix kit (ThermoFisher). Primer sequences were as follows:
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bioRxiv - Cell Biology 2021Quote: ... and a 7500 Real-Time PCR Detection System (Applied Biosystems, ABI). The primers were synthesized by Invitrogen and are shown in supplementary Table 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... using the ABI 7500 Real-Time PCR Detection System (Applied Biosystems). The sequences of primers used are listed in (Supplementary Table 5) ...
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bioRxiv - Cancer Biology 2024Quote: ... Real time PCR was performed using SYBR Green detection (Applied Biosystems) and was performed and analysed using Viia7 Real Time PCR System (Life Technologies ...
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bioRxiv - Immunology 2024Quote: ... and the QuantStudio 5 Real-Time PCR Detection System (Thermo Fisher). The Throat Swab sample was analyzed for SARS-CoV-2-specific RNA by quantitative RT-PCR (qRT-PCR) ...
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bioRxiv - Cell Biology 2020Quote: ... Target mRNAs were amplified by quantitative real time PCR in 20 µl reactions on the real-time PCR system 7900HT Sequence Detection System ABI PRISM (Applied Biosystems) using one step TaqMan RT enzyme and TaqMan RT-PCR Mix (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2024Quote: ... Real-time PCR amplification and fluorescence detection were carried out using a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher). An initial step (95 °C ...
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bioRxiv - Microbiology 2020Quote: ... The RT-qPCR was assessed on a Real-time PCR StepOne Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). PCR thermal cycling conditions had an initial step at 94 °C for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... Real-time polymerase chain reaction (RT-PCR) was performed on a 7500 Real-time PCR System (Applied Biosystems, Carlsbad, CA, USA) with Universal SYBR Green Master Mix (4913914001 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We did One-step real-time RT-PCR (Step-One Plus real-time PCR thermocycler, Applied Biosystems, Foster City, CA, USA), using total nucleic acids extracted by Trizol® (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Real-time quantitative PCR (RT-qPCR) reactions were performed in the Quant Studio6 Flex Real Time PCR System (ABI, Thermo Fisher) using the SYBR Select PCR Master Mix (ABI ...
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bioRxiv - Microbiology 2022Quote: ... Real-time RT-PCR was performed in 96-well plates using an ABI PRISM 7300 Real-time PCR System (Applied Biosystems) and the relative gene expression for the different genes was calculated from the crossing threshold (Ct ...
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bioRxiv - Genetics 2023Quote: ... Quantitative real-time RT-PCR (qPCR) was performed on the QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA), using the SYBR-Green Master Mix (Applied Biosystems ...
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bioRxiv - Genomics 2023Quote: The real-time quantitative PCR (RT-qPCR) assays were performed using the QuantStudieo 5™ Real-Time PCR System (Applied Biosystems). Amplifications were carried out in 25 μL reaction solutions containing 12.5 μL 2x PerfeCTa SYBR green SuperMix (2X ...
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bioRxiv - Cell Biology 2024Quote: ... Gene expression levels were determined by quantitative real-time polymerase chain reaction (RT-PCR) (StepOnePlusTM Real-Time PCR System; Applied Biosystems) with the 2-ΔΔCT method [35] ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative RT-PCR was performed using QuantiTect Probe RT-PCR Kit (Quiagen®) in a StepOne™ Real-Time PCR System (Thermo Fisher Scientific). Amplifications were carried out in 25 µL reaction mixtures containing 2X reaction mix buffer ...
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bioRxiv - Neuroscience 2020Quote: ... The viral RNA loads were determined by real time RT-PCR with the Taqman one-step RT-PCR master kit (Applied Biosystems, CA). The following specific primers and probe of NCSU1 FIV gag region were used ...
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bioRxiv - Microbiology 2022Quote: ... Real-time PCR was performed using quantitative real-time PCR (PikoReal real-time PCR, Thermo Fisher Scientific). PCR (three replicates ...
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bioRxiv - Molecular Biology 2021Quote: ... The real-time RT-qPCR reactions were performed on an ABI StepOnePlus Real-Time PCR System (Applied Biosystems). The NcVATPase primers used for real-time RT-qPCR were 5′-GGAGTTCAATCCCTCCAACA-3′ and 5′-GGGCTCAAGCATGATTTTGT-3′ ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time quantitative PCR (RT-qPCR) was performed using a 7500 Fast Real-Time system machine (Applied Biosystems). Gene expression was normalized to the expression of the reference gene Actin beta (ACTB).
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bioRxiv - Neuroscience 2020Quote: ... Real-time RT-PCR was performed with the EXPRESSS One-Step SuperScript qRT-PCR kit (Thermo Fisher Scientific) using gene-specific primers (Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR (RT-PCR) was conducted using the TaqMan PCR Master Mix (Applied Biosystems), with glyceraldehyde-3-phosphate dehydrogenase (gapdh ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with hsa-miR-3p mimic (Invitrogen) or a control mimic at 5 nM final concentration using Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... or hsa-miR-106b-3p mirVana™ miRNA Mimic (Ambion) at final concentration of 5 nM ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative RT-PCR reaction in real time was done on an ABI Prism ® 7500 Sequence Detection System (Applied Biosystems), using System SYBR-green fluorescence (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative real-time RT-PCR (RT-qPCR) was performed using EvaGreen (Solis BioDyne) on an ABI StepOnePlus™ Real-Time PCR System (Applied Biosystems). tubulin levels were measured and used as an internal control for RNA amount in each sample ...
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bioRxiv - Immunology 2021Quote: ... Real-time RT-PCR was used for mRNA quantification (7500 Fast Real-Time PCR system, Applied Biosystems Inc., Foster City, California, USA). All primers were designed to cover exon-exon boundaries to avoid amplification of genomic DNA ...
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bioRxiv - Immunology 2019Quote: ... Real-time RT-PCR was used for mRNA quantification (7500 Fast Real-Time PCR system, Applied Biosystems Inc., Foster City, California, USA). All primers were designed to cover exon-exon boundaries to avoid amplification of genomic DNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Real-Time quantitative PCR (RT-qPCR) was performed on the QuantStudio™ 5 Real-Time PCR System 384-well (Applied Biosystems, #A28140) using SenSMix™ SYBR® No-ROX buffer (Meridian Bioscience) ...
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bioRxiv - Microbiology 2023Quote: ... real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) using Taqman probe (Mm00458221 ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative real-time reverse-transcription PCR (RT–qPCR) assays were performed on a 7500 Fast Real-Time PCR system (Applied Biosystems, USA). The primer sequences were listed in S1 Table ...