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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for RAB41 Member RAS Oncogene Family RAB41 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Anti-rabbit pan-Ras (#PA5-85947; Thermo Fisher Scientific) and anti-rabbit Phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked with 1% BSA (Thermo Scientific, cat# 37520, for phospho-Src family) or 3% milk (Lab Scientific ...
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bioRxiv - Cell Biology 2021Quote: N-terminally MBP-tagged (pTHMT) FUS CHOP fusion type II and CHOP oncogene were expressed in Escherichia coli BL21 Star (DE3) cells (Life Technologies). Bacterial cultures were grown to an optical density of 0.7–0.9 before induction with 1 mM isopropyl-b-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Developmental Biology 2019Quote: ... then left in molten paraffin (RA Lamb Wax – Fisher Scientific) at 60°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... 1% N2 supplement and 2% B27 with RA supplement (ThermoFisher) and were then fixed and analyzed by immunostaining.
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bioRxiv - Neuroscience 2023Quote: ... and 10 μM all-trans retinoic acid (RA; ThermoFisher Scientific) for 48 hours ...
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bioRxiv - Genomics 2019Quote: ... The biotinylated primers and undigested library members were removed using Dynabeads M-270 Streptavidin (Thermo Fisher Scientific, Waltham, MA), using the recommended “Immobilize nucleic acids” protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... A pool of 14 siRNAs was used to target transcripts of the KDM5 family (Thermo Fisher 4392420 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The concentration of the purified BCL-2 family proteins was determined by BCA assay (ThermoFisher Scientific), and they were flash-frozen in liquid nitrogen and stored at -80⍛C.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... A pool of 16 siRNAs was used to target the transcripts of the KDM4 family (Thermo Fisher 4392420 ...
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bioRxiv - Genetics 2022Quote: ... DNA from the affected siblings in family C was exome sequenced using Ampliseq whole exome kit (ThermoFisher Scientific) and run on a Proton Sequencer ...
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bioRxiv - Microbiology 2021Quote: Relative compositions of community members (corresponding to Extended data Fig. 10) were measured by Q-PCR using PowerUp SYBR Green Master Mix (Thermo Fisher, A25742), 400 nM primers ...
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bioRxiv - Neuroscience 2022Quote: ... Candidates of variants identified in exome sequencing were evaluated in other affected and unaffected members by Sanger sequencing on an ABI Prism 3130 DNA Analyzer (Applied Biosystems Waltham, MA).
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bioRxiv - Cancer Biology 2021Quote: ... HRAS RAS- less MEFs was cultured in 2.5 μg/ml of puromycin (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2023Quote: Cultured cells were treated with all-trans retinoic acid (RA, 1 µM, Fisher Scientific), high affinity pan-RAR antagonist AGN-193109 (AGN ...
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bioRxiv - Cancer Biology 2023Quote: ... SiRNA oligos were transfected into NHA and NHA-RAS cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The total load of bacteria and bacterial families were measured on the 7500 Fast Real-Time PCR system (Applied Biosystems) in 10 μL reactions using Fast SYBR™ Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We subsequently placed two frozen individuals from each family and treatment group (n = 9 families * 2 treatments (+/− supplemental thiamine) * 2 individuals = 36) into 10 volumes of RNAlater-ICE (Invitrogen) pre-chilled to −80 °C and allowed the samples to reach −20 °C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... the coding sequence of Cmb-RA was cloned into the pDONR Zeo entry vector (Invitrogen) and then transferred into the pMT-TurboID-V5 vector (a gift from Norbert Perrimon ...
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bioRxiv - Cancer Biology 2022Quote: ... NMR and mouse SV40-Ras skin fibroblasts were cultured in DMEM high glucose (#41965-039; Gibco) supplemented with 15% fetal bovine serum (#F7524-500ML ...
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bioRxiv - Evolutionary Biology 2019Quote: ... or HDHD-NDA-RA-pGL4.27 along with 500 ng pRL-CMV (Renilla reporter plasmid) via electroporation (Neon Transfection System, Invitrogen). Firefly and Renilla luciferase activity were analyzed using the Dual-Glo Luciferase Assay System (Promega ...
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bioRxiv - Neuroscience 2023Quote: Full length vnd-RA was amplified via PCR from cDNA and cloned into a pRSET bacterial expression vector (ThermoFisher) in frame with an N-terminal 6x-His tag ...
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bioRxiv - Neuroscience 2024Quote: ... Soluble protein samples were prepared with NuPAGE™ lithium dodecyl sulfate (LDS) and reducing agent (RA) (Thermo fisher Scientific) and boiled at 95°C for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... IMR90 ER:RAS cells has been transiently transfected with 500ng of sin-lncRNA or empty pcDNA3.1 plasmid using Lipofectamine 2000 (Invitrogen) in opti-MEM medium (GIBCO) ...
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bioRxiv - Systems Biology 2020Quote: ... Activated RAS or 20µg of total cell protein were separated using NuPAGE Novex Bis-Tris gels (Invitrogen/Thermo Fisher Scientific) and transferred to PVDF membrane using an iBlot™ 2 dry blotting system (Invitrogen/Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2020Quote: ... Activated RAS or 20µg of total cell protein were separated using NuPAGE Novex Bis-Tris gels (Invitrogen/Thermo Fisher Scientific) and transferred to PVDF membrane using an iBlot™ 2 dry blotting system (Invitrogen/Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 50 µg/ml gentamycin. HMLE and HMLE-Ras (kindly provided by Dr. Robert Weinberg, Whitehead Institute) were cultured with DMEM/F-12 (Gibco), 10% FBS ...
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bioRxiv - Systems Biology 2020Quote: The datasets for the impact of diet on RA (MSV000084556) and Alzheimer’s disease (MSV000085256) were collected with similar methods on a Q-exactive Orbitrap mass spectrometer (Thermo Scientific). The Alzheimer samples include Alzheimer’s Disease and elderly controls ...
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bioRxiv - Developmental Biology 2022Quote: ... at room temperature and transitioned into wax in a 1:1 molten paraffin:histosol solution (30 min/wash, twice) and placed in molten paraffin (RA Lamb Wax – Fisher Scientific) at 60°C overnight ...
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bioRxiv - Genetics 2023Quote: ... differentiation was initiated by switching to DMEM-F12/Neurobasal media (2:1) supplemented with N2 and retinol-free B27 (N2B27 RA−; Gibco). For the first ten days ...
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bioRxiv - Bioengineering 2023Quote: ... Antibodies against the following markers were used in the RA and EAE mouse models: INOS (BUV737, Cat#367-5920-82, Invitrogen), CD11c (BV421 ...
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bioRxiv - Genetics 2022Quote: Total RNA was extracted from ∼2 mg of right atrium (RA) tissues of 1-year-old Dnajb6+/- heterozygous mutant hearts and WT sibling controls using Trizol reagent (ThermoFisher Scientific) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... IL-1-Ra plasma levels were determined of 20 patients positive and 12 patients negative for IL-1-Ra-Abs with a commercially available ELISA kit (Invitrogen/ThermoFisher #BMS2080) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... UM was changed for 2 days to an endothelial cell medium supplemented with retinoic acid (EC+RA: human Endothelial-SFM (Life Technologies), 1% platelet-poor plasma derived bovine serum (Alfa Aesar) ...
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bioRxiv - Genetics 2019Quote: ... primers (sequences available upon request) were designed to amplify Ras sequences and the Invitrogen Gateway BP Clonase II Enzyme Mix (ThermoFisher Scientific) was used to insert these sequences into the Gateway entry vector pDONOR221 (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... The RAS protein bands were visualized using an enhanced chemiluminescence (ECL) system according to the manufacturer’s instructions (Thermo Fisher Scientific, USA).
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bioRxiv - Immunology 2021Quote: ... IL-1-Ra plasma levels were determined of 20 patients positive and 12 patients negative for IL-1-Ra-Abs with a commercially available ELISA kit (Invitrogen/ThermoFisher #BMS2080) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and at 30 DIV by neurosphere medium (Advanced DMEM/F12, 1x B27 -RA, 1x L-glutamate, 1x NEAA, 1x P-S [all from Thermo Fisher Scientific]) supplemented with 20 ng/ml bFGF2 and 20 ng/ml epidermal growth factor (EGF ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted and purified from synovial tissue (a total of 7 samples from 7 different RA patients) and cultured cells using Trizol reagent (Life Technologies, Carlsbad, CA, USA), followed by cDNA synthesis using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara-Bio ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RAS activity was determined by the Active Ras Pull-Down and Detection Kit according to the manufacturer’s instructions (16117, Thermo Fisher Scientific, Lafayette, CO, USA).
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bioRxiv - Neuroscience 2023Quote: ... CFA was prepared by adding 8 mg/ml of heat– killed Mycobacterium tuberculosis H37 Ra (Difco, catalog no. 231141) to incomplete Freund’s adjuvant (Thermo Fisher Scientific, catalog no. 77145). MOG35-55 peptide (2 mg/ml in PBS ...
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bioRxiv - Cell Biology 2020Quote: Secondary antibodies: Alexa Fluor secondary antibodies (Invitrogen) were used at a dilution of 1:1,000 ...
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bioRxiv - Biochemistry 2022Quote: ... and secondary antibody (Invitrogen anti-mouse antibody) binding ...
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bioRxiv - Developmental Biology 2023Quote: ... For antibodies we used a His antibody (6x-His Tag Monoclonal Antibody, ThermoFisher), a MBP antibody (Anti-MBP Monoclonal Antibody ...
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bioRxiv - Synthetic Biology 2019Quote: ... An anti-6His antibody (6x-His Tag Polyclonal Antibody) and an anti-HA antibody (HA Tag Polyclonal Antibody) were purchased from Invitrogen. Western blot analysis was conducted as described previously 45.
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bioRxiv - Cancer Biology 2023Quote: ... 90 µL of supernatant was mixed with 410 µL of IP Dilution Buffer and antibodies (Positive antibody: 10 µL; Negative antibody: 2 µL; FoxO3a antibody: 8 µL (Cat. #720128, Invitrogen)) and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Dilutions of primary antibody and secondary antibody (ThermoFisher) incubation varied depending on experiments ...
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bioRxiv - Neuroscience 2021Quote: ... All secondary antibodies were Alexa Fluor antibodies (Invitrogen) used at 2 μg/mL.
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bioRxiv - Developmental Biology 2022Quote: ... rabbit polyclonal Pax2 antibody (Invitrogen antibodies, 1:50), rabbit polyclonal Sall1 antibodies (abcam ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibodies were Alexa Fluor antibodies (ThermoFisher Scientific) and were used at a concentration of 1:500.