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Citations for Thermo Fisher :
51 - 100 of 5029 citations for LACTB2 Human GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... and the GTP-loaded Rac pulled down from the total protein lysates using a Rac1 Activation Kit (GST-human Pak1-PBD, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Integrity of the GST tag was subsequently checked via western blotting of the purified recombinant protein using anti-GST antibodies (Invitrogen 71-7500) [1:1,500 dilution] ...
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bioRxiv - Molecular Biology 2023Quote: ... GST pull-down of PARylated proteins using GST-tagged RNF146(100-182) or GST-tagged AF1521(K35E/Y145R) was done using the Pierce GST protein interaction pull-down kit (Thermo Fisher Scientific). Glutathione agarose resin (50μl ...
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bioRxiv - Cancer Biology 2022Quote: ... Purification was carried on GST affinity resin (Thermo Scientific) and reduced glutathione was used for protein release ...
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bioRxiv - Biophysics 2022Quote: ... Anti-GST Alexa488-conjugated antibody was purchased from ThermoFisher Scientific (Catalog number ...
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bioRxiv - Cell Biology 2020Quote: ... GST-NtRac5 were immobilized on glutathione-agarose (Thermo Fisher Scientific) and incubated with purified recombinant MBP-AtPIP5K2 protein for 60 min at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... the GST-RNF38RING was incubated with glutathione agarose (Thermo Fisher). The UBC13~Ub conjugate was mixed with GST-RNF38RING (60 μM ...
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bioRxiv - Neuroscience 2020Quote: ... coli expression vector pDEST15 with GST tag Gateway Vector (Invitrogen). MYC-LRRK2-WT was a gift from Dr ...
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bioRxiv - Biochemistry 2022Quote: ... and anti-GST tag monoclonal antibody MA4-004 (ThermoFisher Scientific). The primary antibodies were detected with secondary anti-rabbit and anti-mouse antibodies (LI-COR Biosciences) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 25 nM Alexa-488 conjugated GST antibody (Fisher Scientific, #10368552), 5 mM DTT ...
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bioRxiv - Immunology 2019Quote: ... and anti-glutathione-S-transferase (GST) antibody (ThermoFisher 71-7500). Antibody-bound complexes were collected ...
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bioRxiv - Cancer Biology 2021Quote: ... Anti-HA.11 antibody was purchased from Biolegend and antibodies against V5 and GST tags were from ThermoFisher.
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bioRxiv - Cell Biology 2020Quote: ... and mouse anti-GST (1:500, Thermo Fisher, MA4-004). For visualisation ...
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bioRxiv - Biochemistry 2020Quote: ... and anti-GST tag monoclonal antibody MA4-004 (ThermoFisher Scientific). The primary antibodies were detected with secondary infrared anti-rabbit and anti-mouse antibodies (LI-COR Biosciences) ...
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bioRxiv - Molecular Biology 2023Quote: ... or anti-GST (1:1000) (cat#8-326, Thermofisher scientific) antibodies ...
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bioRxiv - Microbiology 2024Quote: ... and anti-GST tag monoclonal antibody MA4-004 (ThermoFisher Scientific). The primary antibodies were detected with secondary infrared anti-rabbit and anti-mouse antibodies (LI-COR Biosciences) ...
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bioRxiv - Biophysics 2024Quote: ... or GST using Lipofectamine™ 3000 Transfection Reagent (Invitrogen, L3000001), and after 48h ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-tagged NgAgo variants were purified by glutathione agarose (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... for His-tagged proteins or GST-agarose resin (Thermo Fisher Scientific) for GST-tagged proteins ...
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bioRxiv - Cell Biology 2020Quote: ... Millipore] and isolated using Pierce GST Spin Purification Kit (Thermo Scientific). GST-p85-S6K1 expression vector48 was obtained from Addgene (Plasmid #8466 ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was incubated with GST-TAG antibody (1:1000, ThermoFisher) or anti-MC4 (rabbit ...
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bioRxiv - Microbiology 2019Quote: ... GST-tag and 3xFLAG-tag fusions were achieved using pDEST27 (Invitrogen) and pCI-neo-3xFLAG vector ...
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bioRxiv - Molecular Biology 2020Quote: ... The GST tag was removed using HRV 3C protease (Thermo Scientific) with an enzyme to substrate ratio of 1:100 at 4°C for 16 hrs ...
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bioRxiv - Cell Biology 2023Quote: ... anti-GST mouse monoclonal (Invitrogen, #MA4-004, 1:5000 in WB), anti-PLK4 rabbit polyclonal (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... and polyclonal anti-GST (Thermo Fisher Scientific, Waltham, MA, USA, CAB4169) antibodies ...
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bioRxiv - Biochemistry 2019Quote: ... and CMG2-GST was affinity purified with Glutathione Superflow Agarose (Thermo Fisher). Glutathione was removed and CMG2 exchanged into HBS-T with 50% glycerol using Sephadex G50 (GE Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were incubated with 200μl of GST-Sepharose beads (Thermo Scientific) and washed 6 times with CHAPS buffer containing 0.5 M NaCl and 3 times with CHAPS buffer containing 5 mM MgCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μl (0.21 μg) of PKR(252-551)-GST enzyme (Thermo Scientific) was added ...
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bioRxiv - Biochemistry 2021Quote: ... The filtered lysate was then added to GST-beads (Thermo Fisher Scientific) pre-equilibrated in binding buffer and incubated for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... GST proteins were captured on Pierce glutathione magnetic beads (Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... except for passage of the final prep through a GST column (ThermoFisher). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing GST-Cdc6 were treated with 200 U PreScission protease (ThermoFisher) for 2 h on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... GST fusion proteins were expressed in Escherichia coli BL21 Star (DE3) (Invitrogen) or C41 (86 ...
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bioRxiv - Microbiology 2020Quote: ... The vector only encoding GST was transformed into BL21 DE3 (ThermoFisher Scientific). Plasmids encoding GST-YidC1CT or GST-YidC2CT were transformed into BL21 Star™ (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... was incubated with purified GST-tagged IKKi (catalog # PV4875; Thermo Fisher Scientific) in buffer A containing 20 mM Tris [pH 7.5] ...
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bioRxiv - Cancer Biology 2023Quote: ... GST-ISG15 was expressed in Escherichia coli BL21(DE3) (Thermo Fisher, EC0114) at OD600 0.6-0.8 with 0.1 mM IPTG and 20°C and lysed in the lysis buffer by sonication ...
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bioRxiv - Cancer Biology 2022Quote: ... bacterially expressed GST-p21 bound to glutathione Sepharose beads (Thermo Scientific, 16100) were incubated with His-RanBPM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Then GST was removed using 20 U/mg protein of Actev (Invitrogen) according to the instructions of the manufacturer.
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bioRxiv - Cell Biology 2024Quote: ... To detect PI(4,5)P2 a GST Tag Antibody (MA4-004; Invitrogen) was used ...
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bioRxiv - Cancer Biology 2019Quote: ... using the Pierce™ GST protein interaction pull-down Kit (21516, Thermo Fisher), as previously described [49 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Assay mixes with 1 nM GST-tagged GR (aa 521-777, Invitrogen #PV4689), 25 nM Alexa-488 conjugated GST antibody (Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific). On-bead recombinant proteins were washed with the respective lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein binding was detected with Alexa488-conjugated α-GST antibody (Invitrogen A-11001). Arrays were scanned using a GenePix 4400A microarray scanner (Molecular Devices) ...
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bioRxiv - Neuroscience 2022Quote: ... RAP-GST was eluted in Buffer A with 25 mM glutathione (Fisher Scientific). Fractions confirmed by Coomassie were dialyzed in a dialysis buffer (20 mM HEPES ...
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bioRxiv - Plant Biology 2023Quote: ... The GST-tagged proteins were resuspended in phosphate-buffered saline (PBS) (Thermo Fisher) containing 1 mM dithiothreitol (DTT ...
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bioRxiv - Cell Biology 2022Quote: ... The GST- fusion proteins were purified under native conditions using glutathione agarose (ThermoFisher Scientific) and the LRRCC1 fragments were recovered by Tev protease cleavage and dialyzed before rabbit immunization (Covalab) ...
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The circadian clock gene circuit controls protein and phosphoprotein rhythms in Arabidopsis thalianabioRxiv - Systems Biology 2021Quote: ... raised against amino acids 566-651 (42) and anti-GST from mouse (Thermo Scientific), both at a dilution of 1:1,000 overnight ...
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bioRxiv - Cell Biology 2021Quote: Purified recombinant GST-tagged CDK1-cyclin A2 and His6-tagged CDK1-cyclin B1 (Invitrogen) were stored in 150 mM NaCl ...
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bioRxiv - Genomics 2022Quote: ... The 15N-GST-H4 peptide fusion was purified on glutathione agarose resin (Thermofisher Scientific), cleaved with PreScission Protease (16 hr incubation at 4°C) ...
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bioRxiv - Cell Biology 2019Quote: ... GST-PI31 was expressed in BL21 Star (DE3) E.coli cells(Thermo Fisher Scientific, #C601003), and purified using Glutathione Sepharose 4B beads (GE Healthcare ...