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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for Eukaryotic Peptide Chain Release Factor GTP Binding Subunit ERF3A GSPT1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Plasmids encoding both the heavy chain and light chain for each antibody were co-transfected into FreeStyle 293-F cells (Invitrogen) using polyethylenimine ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM GTP (#R0461; Thermo Fisher Scientific) was added and the reaction volume was transferred to a pre-heated (37 dC ...
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bioRxiv - Genetics 2023Quote: ... 1 mM GTP (Thermo Scientific, cat#R0461), 5% PEG8000 (PERFEMIKER ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 mM GTP (Thermo Fisher Scientific, R0461), 2 μM TSO (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... DMT1 antibody and DMT1 siRNA duplex chains were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies: Rabbit anti-J Chain (SP105, 1:100, Thermo Fisher Scientific), Goat anti-Rabbit IgG1 ...
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bioRxiv - Cell Biology 2021Quote: Rap1-GTP and Rho-GTP were respectively analyzed by means of the active Rap1 (Cat. #16120, Thermo Fisher Scientific) and Rho (Cat ...
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bioRxiv - Immunology 2024Quote: Antibody heavy chain (HC) and light chain (LC) sequence pairs generated from 10X Genomics protocols were synthesized (Geneart, Thermo Fisher Scientific) and cloned into expression vectors containing an IgG1 HC or LC constant region ...
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bioRxiv - Cell Biology 2022Quote: ... Binding of indicated antibodies to Dynabeads Protein A (Invitrogen) was performed overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Binding of indicated antibodies to Dynabeads Protein A (Invitrogen) was performed overnight ...
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bioRxiv - Microbiology 2023Quote: ... streptavidin-binding peptide) was cloned in pcDNA3.1(pcDNA:Rv2067cSFB) and transfected into HEK293T cells using Lipofectamine 2000 (Invitrogen). A control of HEK293T cells transfected with pcDNA SFB was kept ...
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bioRxiv - Microbiology 2024Quote: ... The extracted peptides were transferred to clean low-protein binding microtubes and dried using SpeedVac (Thermo Scientific). The dried samples were resolubilized in 5% ACN containing 0.1% formic acid and desalted using C18 Zip Tips™ columns (Sulpelco ...
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bioRxiv - Microbiology 2020Quote: ... Eukaryotic mRNA was depleted using Dynabeads covalently linked with oligo dT (ThermoFisher). The in vitro samples underwent the same treatment with Dynabeads to reduce any potential biases this procedure might introduce to the downstream sequencing ...
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bioRxiv - Microbiology 2022Quote: ... The Nbs were finally cloned into the pcDNA3.4 eukaryotic expression vector (Invitrogen), which were transfected into 293F cells (cat.# R79007 ...
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bioRxiv - Microbiology 2020Quote: ... eukaryotic 18S assay (Hs99999901_s1, Applied Biosystems, Thermo Fisher Scientific, Carlsbad, California, USA), was run with each sample to confirm successful DNA extraction and lack of PCR inhibitors ...
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bioRxiv - Cell Biology 2023Quote: All eukaryotic cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco) containing 10% FCS (Seromed) ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was depleted using the eukaryotic Ribo-minus kit (Ambion; # A1083708) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Plasmids pcDNA3.4 comprising heavy chains and light chains of antibodies were transiently expressed in ExpiCHO-S cells using the ExpiCHO™ expression system (Thermo Fisher). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were co-transfected by polyethylenimine-transfection to Expi293F™ cell (ThermoFisher, A14527). Cells were cultured at 36.5°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... GTP and dATP from Thermo Scientific (Dreieich, Germany), and CNDAC-TP from Jena Bioscience GmbH (Jena ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... GTP and ATP were purchased from Fisher Scientific. The RNA oligonucleotides (template-loop-primers ...
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bioRxiv - Biochemistry 2024Quote: ... To load myrArf1 with mant-GTP (ThermoFisher, M12415), 1 mM myrArf1 was incubated at 30°C for 40-60 min in 25 mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... antibodies in 1X FoxP3/transcription factor permeabilization solution (Thermofisher) for 60 minutes at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibody binding was detected using Alexa fluorophore-conjugated secondary antibodies (ThermoFisher Scientific). For BrdU immunostaining ...
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bioRxiv - Biophysics 2023Quote: ... the full chain antibodies were dialyzed using 10K dialysis devices (Thermo Scientific™ Slide-A-Lyzer™ MINI Dialysis Devices ...
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bioRxiv - Biophysics 2024Quote: Single-chain antibody scFv16 was expressed in High Five insect cells (Invitrogen) using the Bac-to-Bac baculovirus expression system ...
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bioRxiv - Immunology 2023Quote: ... for murine and Fc Receptor Binding Inhibitor Polyclonal Antibody (Invitrogen) for human samples and subsequently stained with live/dead cell exclusion dye (Zombie Dyes ...
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bioRxiv - Biochemistry 2020Quote: ... Captureselect Biot A-light chain κ HU and light chain λ HU antibodies and Myone Streptavidin T1 beads were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and 18S as the reference gene (Eukaryotic 18S rRNA Endogenous Control, Applied Biosystems). The qPCR data are presented as relative expression levels calculated by ΔΔCt (where ΔCt is determined by the difference cycles threshold of the target gene and the reference gene).
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bioRxiv - Molecular Biology 2023Quote: ... Cycle threshold values were normalized using Pan Eukaryotic 18S (ThermoFisher Scientific, MA, USA), transformed using the 2-ΔCT method ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal antibodies against binary toxin subunits CDTa and CDTb and anti-Clostridia GDH monoclonal mouse antibody (Clone: J44D, Invitrogen) were obtained from Merck ...
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bioRxiv - Genetics 2020Quote: ... Intracellular staining (for experiments in which the NF-κB binding site containing rs6927172 was deleted) was performed using the eBioscience Foxp3 / Transcription Factor Staining kit (ThermoFisher) and a Phospho-IkB alpha (Ser32 ...
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bioRxiv - Plant Biology 2020Quote: Regions screened for transcription factor binding via Yeast One-Hybrid were first inserted into pENTR 5’TOPO TA entry vector (Thermofisher) and subsequently placed into the pMW2 and pMW3 destination vectors containing HIS3 and LACZ marker genes respectively (Deplancke et al. ...
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bioRxiv - Systems Biology 2022Quote: ... Monoclonal IgG2a antibody to α-adaptin (α subunit of AP2) (AB_2056321) was from ThermoFisher (Waltham, MA). Mouse monoclonal antibodies to EEA1 (AB_397829) ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... Antibody binding was detected by fluorophore-conjugated highly cross-adsorbed secondary antibodies (ThermoFisher Scientific), and nuclei were stained with Hoechst 33342 (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: KRAS was loaded with GTP nucleotide by incubating 100 µM protein with 10 mM EDTA and 500 µM ultra-pure GTP (Thermo Scientific, R0461) for 2 hrs at room temperature with slow shaking (150 rpm) ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were transiently transfected with plasmids expressing GSPT1-HA wild type and variants (6 µg) using lipofectamine 3000 according to manufacturer’s protocol (Thermo Fisher Scientific). After 48 h ...
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bioRxiv - Biochemistry 2022Quote: ... Guanosine-5’-triphosphate (GTP) was obtained from Thermo Scientific. 2’-deoxythymidine-5’-[α-thio]-triphosphate (dTTPαS ...
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bioRxiv - Neuroscience 2023Quote: ... 0.3 Na-GTP and 0.03 Alexa Fluor 594 (ThermoFisher) (310 mOsm/kg ...
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bioRxiv - Genomics 2022Quote: ... Antibody heavy chains were prepared for conjugation using SiteClick™ azide modification (Invitrogen) of carbohydrate domains ...
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bioRxiv - Molecular Biology 2024Quote: ... Expression of the mouse CD8 α-chain was determined with fluoresceinated antibodies (Thermofisher). Secretion of the human coagulation factor VIII was determined by EliSpot assays ...
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bioRxiv - Neuroscience 2024Quote: ... and HRP-conjugated goat α-human IgG kappa light chain antibodies (Thermo Scientific).
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bioRxiv - Neuroscience 2024Quote: ... we used a pair of rabbit anti-human IgG heavy chain antibodies (Invitrogen) and HRP-conjugated goat α-human IgG kappa light chain antibodies (Thermo Scientific).
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bioRxiv - Neuroscience 2022Quote: ... Antibody binding was revealed by the Enhanced Chemiluminescence System (Life Technologies) on X-Ray film (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection of DNA into eukaryotic cells was performed in a serum-free medium (Gibco) with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... and lysed using Eukaryotic Lysis Solution (ELS) (50mM Tris HCl, pH7.5 (15567-027, Invitrogen), 150mM NaCl (S23020 ...
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bioRxiv - Cell Biology 2024Quote: ... clathrin heavy chain (ThermoFisher), caveolin 1 (Dharmacon) ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: ... Antibody binding was visualized with standard Alexa Fluor secondary antibodies (Invitrogen, 1:500 in PBS) except for Pax7 which was detected using a Tyramide SuperBoost Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibody binding was detected using HRP-conjugated anti-mouse or anti-rabbit antibody (Invitrogen) and chemiluminescence (Thermo Scientific) ...