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51 - 57 of 57 citations for DSPE Alkyne since 2020

• Cell type-Specific In Vivo Proteomes with a Multi-copy Mutant Methionyl t-RNA Synthetase Mouse Line

  Rodrigo Alvarez-Pardo, et al., bioRxiv - Cell Biology 2024

  Quote: ... The click reaction was performed for 2 hrs at room temperature using 2 μM of the Alexa647 alkyne (Thermo Fisher Scientific, A10278), in a copper-mediated click reaction with CuSO4 ...
• Intrauterine growth restriction causes cellular, molecular, and behavioral deficits consistent with abnormal dentate gyrus neurogenesis in mice

  Ashley S. Brown, et al., bioRxiv - Neuroscience 2020

  Quote: ... a copper-catalyzed covalent reaction between an azide which is the Alexa Fluor 488 dye and an alkyne which is the EdU (C10339, Invitrogen Corporation, Carlsbad, CA). EdU is capable of crossing the placenta to be taken up by actively dividing NSPCs in the fetal brain ...
• Single-molecule conformational dynamics of a transcription factor reveals a continuum of binding modes controlling association and dissociation

  Wei Chen, et al., bioRxiv - Biophysics 2021

  Quote: ... RelA and p50 were thawed from −80 °C and labeled separately with Click-iT™ Alexa Fluor™ 555 sDIBO Alkyne (FRET donor, Thermo Fisher Scientific) or Click-iT™ Alexa Fluor™ 647 sDIBO Alkyne (FRET acceptor ...
• Hexosamine biosynthetic pathway integrates circadian and metabolic signals to regulate daily rhythms in protein O-linked N-acetylglucosaminylation

  Xianhui Liu, et al., bioRxiv - Molecular Biology 2020

  Quote: ... Samples were azide-labeled using Click-iT O-GlcNAc Enzymatic Labeling System and reacted with biotin alkyne using Click-IT Biotin Protein Analysis Detection Kit (Thermo Fisher Scientific, Waltham, MA) following manufacturer’s protocol ...
• Retinoschisin deficiency induces persistent aberrant waves of activity affecting neuroglial signaling in the retina

  Cyril G. Eleftheriou, et al., bioRxiv - Neuroscience 2021

  Quote: ... using a site-specific antibody labelling kit (SiteClick™ Antibody Azido Modification Kit, Cat: S0026; SiteClick™ Alexa Fluor™ 647 sDIBO Alkyne, Cat: S10906, ThermoFisher Scientific). Following the final wash step in the protein concentrator ...
• First comprehensive identification of proteins with increased O-GlcNAc levels during pressure overload hypertrophy

  Wei Zhong Zhu, et al., bioRxiv - Physiology 2022

  Quote: ... The sample was subsequently labeled with the TAMRA-alkyne dye by using the Click-It™ TAMRA Glycoprotein Detection Kit (Thermo Fisher Scientific, Waltham, MA). The mixture was vortexed for 5 seconds after the addition of each component according to manufacturer’s instructions ...
• The type VII secretion system protects Staphylococcus aureus against antimicrobial host fatty acids

  Arnaud Kengmo Tchoupa, et al., bioRxiv - Microbiology 2020

  Quote: ... and the bacterial pellets resuspended in PBS supplemented with 4 µg/mL Click-iT™ Alexa Fluor™ 488 sDIBO alkyne (Life Technologies LTD, UK). After incubation at 25°C for 1h with shaking ...