Labshake search
Citations for Thermo Fisher :
51 - 100 of 10000+ citations for Cytomegalovirus Glycoprotein B gB Mouse Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2019Quote: ... washed and resuspended in FC buffer with goat-anti-mouse Alexa488 (Invitrogen; 1:400) for an additional 15 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... Detection was performed with secondary goat anti-mouse IgG Fc HRP antibody (Invitrogen, A16084) and WesternBright Sirius chemiluminescent HRP conjugate (advansta ...
-
bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% FCS (FCS, Thermo Fisher Scientific), 100 U ml−1 penicillin ...
-
bioRxiv - Cell Biology 2021Quote: ... and were cultured at 37°C under 5% CO2 in DMEM supplemented with 10% FCS and 100 μg/mL of hygromycin B (Invitrogen, 10687010).
-
bioRxiv - Immunology 2021Quote: ... and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher. Cell counting was performed with CountBright Absolute Counting Beads (Thermo Fisher ...
-
bioRxiv - Immunology 2019Quote: ... and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher. Antibodies against human CD62L (clone DREG-56) ...
-
bioRxiv - Microbiology 2023Quote: The HSV-1 ANG gB PCR product was first subcloned into the pCR4-TOPO vector (Invitrogen) by TA cloning ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... and a lab-made cytomegalovirus promoter-driven enhanced green fluorescent protein (GFP) reporter construct using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). On day three ...
-
The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: The coding region of each opsin transcript was amplified from eye cDNA and subcloned in a modified pFRT-TO expression vector cassette derived from pcDNA5 and containing the human cytomegalovirus (CMV) immediate early promoter (Invitrogen, USA). The expression plasmid was modified to include a C-terminal tag by the monoclonal antibody FLAG epitope sequence (DYKDDDDK) ...
-
bioRxiv - Physiology 2020Quote: ... and a non-targeting pre-miRNA under bicistronic control of the cytomegalovirus (CMV) promoter in the pcDNA6.2GW/EmGFP-miR plasmid (Invitrogen, Carlsbad, CA). The Fbxl22 RNAi plasmid encodes EmGFP and an artificial pre-miRNA targeting the full length gene of mouse Fbxl22 under bicistronic control of the CMV promoter ...
-
bioRxiv - Immunology 2020Quote: ... ECD region of mouse CLEC2 was cloned into mammalian expression vector pFuse-rIgG-Fc (Invitrogen). The construct was used to stable transfect HEK-293T cells with PEI method ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were blocked with anti-mouse CD16/32 (Fc-block, Invitrogen, San Jose, CA, USA) for 10 minutes at 4°C followed by staining with antibody cocktail containing Pacific-Blue-conjugated anti-mouse CD45 (clone 30-F11 ...
-
bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen, Cat. No. 31437). MLV P30 protein within the pseudovirus capsid was detected using a rabbit anti-MLV-P30 polyclonal antibody (Origene ...
-
bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen, Cat. No. 31437). Beta-actin was used as an internal control.
-
bioRxiv - Immunology 2022Quote: ... Fc-receptors were blocked with anti-mouse CD16/CD32 (20 µg/ml, clone 93; Invitrogen) for 20min ...
-
bioRxiv - Bioengineering 2023Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Goat anti-mouse IgG1 and IgG2c Fc secondary antibodies (ab97250 ...
-
bioRxiv - Bioengineering 2023Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Goat anti-mouse IgG1 and IgG2c Fc secondary antibodies (ab97250 ...
-
bioRxiv - Immunology 2023Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Alexa Fluor 488 Anti-alpha 1 Sodium Potassium ATPase antibody (ab197496) ...
-
bioRxiv - Immunology 2021Quote: ... The primary antibodies used were as follows: P-glycoprotein (1:1000, Invitrogen MA1-2652), Caspase-1 (1:2000 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... VSV pseudotyped with EBOV glycoprotein was grown by infecting Vero cells (Ambion, Austin, TX) and then harvesting via filtration of the supernatant through 0.4 µM filters 24-30 hours after infection ...
-
bioRxiv - Genetics 2020Quote: ... and TaqMan Sult1a1 or Ugt1a6a/b Mouse Gene Expression Assay (Mm01132072_m1, Mm01967851_s1, Mm03032310_s1, ThermoFisher). qPCR used 2 min hold at 50 °C then 10min at 95 °CC ...
-
bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FCS (FCS; Invitrogen; Thermo Fisher Scientific, Inc.). When the cultured cells reached 70-80% confluence at 37° C in a humidified 5 %CO2 incubator ...
-
bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FCS (FCS; Invitrogen; Thermo Fisher Scientific, Inc.). When the cultured cells reached 70-80% confluence at 37° C in a humidified 5 %CO2 incubator ...
-
bioRxiv - Immunology 2023Quote: ... These plasmids were co-transfected with MLV (murine leukemia virus)-CMV (cytomegalovirus) luciferase and MLV Gag/Pol plasmids into HEK-293T cells (ATCC CRL-3216) using Lipofectamine 2000 (Thermo Fisher 11668019) transfection reagent following the manufacturer’s recommended protocol ...
-
bioRxiv - Immunology 2022Quote: ... 2% FCS (ThermoFisher), and 50 μg/mL gentamicin (ThermoFisher) ...
-
bioRxiv - Microbiology 2021Quote: ... 4 % FCS (Gibco), 200 units/mL penicillin and 0.2 mg/ mL streptomycin ...
-
bioRxiv - Molecular Biology 2020Quote: ... 10% FCS (InVitrogen) and either 1 µg/mL puromycin (DIvA cells ...
-
bioRxiv - Immunology 2022Quote: ... 10% FCS (Gibco), 1% non-essential amino acid (NEAA ...
-
bioRxiv - Immunology 2021Quote: ... 10% FCS (Gibco) in RPMI ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5% FCS (Invitrogen), 5% human serum (HS) ...
-
bioRxiv - Immunology 2023Quote: ... 2% FCS (Gibco), 25 mM HEPES (Merck ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10% FCS (Gibco), 1X Pen-Strep (Gibco) ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 % FCS (Gibco), 1 % PenStrep (Gibco) ...
-
bioRxiv - Cell Biology 2024Quote: ... 44% FCS (Gibco), and 6% DMSO (Invitrogen) ...
-
bioRxiv - Cell Biology 2024Quote: ... 5% FCS (Gibco), and 1x penicillin-streptomycin (Gibco ...
-
bioRxiv - Immunology 2024Quote: ... 10% FCS (Gibco), penicillin/streptomycin ...
-
bioRxiv - Biochemistry 2021Quote: ... BF and GB peptides were acquired using a Q Exactive™ Plus Orbitrap™ MS (Thermo Scientific) coupled with a Dionex UltiMate™ 3000 nanoHPLC system (Thermo Scientific) ...
-
bioRxiv - Immunology 2022Quote: ... The gB Domain I and Domain I+II were purified using Nickel-NTA resin (Thermo Fisher Scientific), and the gB Domain II was purified with lectin resin (VWR.)
-
bioRxiv - Immunology 2022Quote: The modified mRNAs encoding HCMV gBs were produced as previously described using T7 RNA polymerase (MEGAscript; Ambion) on codon-optimized linearized plasmids 77 ...
-
bioRxiv - Cell Biology 2020Quote: Mouse bone marrow cells were isolated by crushing entire long bones into DMEM/3% FCS (GIBCO). Bone fragments were removed by filtration through a 70 μm filter mesh ...
-
bioRxiv - Cancer Biology 2023Quote: ... and -10 Fcs were assessed by detecting with an anti-mouse IgG-specific secondary (Thermo Fisher) and analyzed via flow cytometry (BD Biosciences).
-
bioRxiv - Microbiology 2020Quote: ... The gel was then stained using Pro-Q Emerald 300 staining for glycoproteins kit (Invitrogen) following the procedure described by the supplier.
-
bioRxiv - Biochemistry 2021Quote: ... The SARS-CoV-2 spike glycoprotein RBD was expressed in Freestyle 293 cells (ThermoFisher, MA) grown in Freestyle 293 media at 37 °C with 8% CO2 and shaking at 135 rpm in a humidified incubator ...
-
bioRxiv - Biochemistry 2023Quote: ... Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl; Thermo Scientific) and alkylated with 10 mM 2-Chloroacetamide (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2024Quote: ... cells were transfected with 30 µg of viral glycoprotein expression plasmid using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: ... charcoal stripped FCS was used (CS-FCS, Gibco® Life Technologies). The human PCa cell line LAPC4 was kindly provided by Zoran Culig (Innsbruck Medical University ...
-
bioRxiv - Cancer Biology 2019Quote: ... charcoal stripped FCS was used (CS-FCS, Gibco® Life Technologies). The human PCa cell line LAPC4 was kindly provided by Zoran Culig (Innsbruck Medical University ...
-
bioRxiv - Neuroscience 2021Quote: ... the mouse ephrin-B3 was cloned into pSecTag-B plasmid (Termo Fisher Scientific, Waltham, USA). Six repeats of the myc epitope were cloned at the carboxyl end of ephrin-B3 ...
-
bioRxiv - Immunology 2020Quote: Splenic mouse B lymphocytes were cultivated in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco, ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... Mouse IgG1 anti-alpha Tubulin Clone B-5-1-2 (1:500, ThermoFisher 32-2500), Rabbit anti-Myc polyclonal (1:500 ...