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Citations for Thermo Fisher :
9801 - 9850 of 10000+ citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody Alexa Fluor 647 (Thermo Fisher Scientific) to assess CM purity as described by Zhang et al.39 Flow cytometry was performed on a Cytoflex Flow Cytometer (Beckman Coulter ...
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bioRxiv - Genetics 2021Quote: ... using a Leica M205C stereomicroscope (190-240X magnification) and collected directly into sterile 0.2 mL PCR flat-cap tubes containing Prep-n-GoTM Lysis buffer (ThermoFisher Scientific, Carlsbad, CA, USA). This was done via a sterile tungsten needle and water-soluble 3MTM adhesive ...
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bioRxiv - Microbiology 2022Quote: ... and expression clones with N-terminal fusion tags were produced simply by Gateway cloning (Gateway™ LR Clonase™ II Enzyme mix, Invitrogen, 11791020). The Lenti-NSP2 was constructed by using the destination vector ...
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bioRxiv - Neuroscience 2021Quote: ... and for bank vole cells NBM complete was switched to DMEM complete supplemented with 1% N-2 supplement (Gibco™ Thermo Fisher, USA). Mouse or bank vole glia cells were plated at 1×105 or 5×104 cells/well ...
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bioRxiv - Neuroscience 2021Quote: ... and for bank vole cells NBM complete was switched to DMEM complete supplemented with 1% N-2 supplement (Gibco™ Thermo Fisher, USA). Mouse or bank vole glia cells were plated at 1×105 or 5×104 cells/well ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was generated using 500 ng of total RNA and the High-Capacity cDNA with Reverse Transcription kit (Applied Biosystems p/n 4368814). QPCR was performed using the Wafergen SmartChip system (TakaraBio ...
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bioRxiv - Genomics 2019Quote: ... Samples were heat-denatured (95°C/ 5 min) and incubated o/n at 37°C with 150 U of λ-exonuclease (λ-exo; Thermo Scientific) in the presence of 40 U of RNAseOUT ...
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bioRxiv - Plant Biology 2020Quote: ... each slide was incubated overnight at 4°C with 100 μL of rabbit polyclonal anti-β-glucuronidase antibody (N-Terminal, 5420, Molecular Probes, Invitrogen) diluted 1:200 in fresh blocking buffer (3% BSA in 1X PBS) ...
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... The LCRN coding sequence was inserted in frame with a N-terminal hexahistidine (His6)-SUMO tag in a Champion pET SUMO expression vector (Invitrogen, Carlsbad, CA, USA) by Gibson Assembly (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... we designed construct PolI-SARS-CoV2-NLuc-N-HDR and ordered this as insert in the pMK-RQ backbone (Invitrogen construct ID 20ACX40P). The resulting plasmid pPolI-SARS-CoV2-NLuc-N was digested with XbaI prior to transfection to eliminate background (read-through ...
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bioRxiv - Cell Biology 2021Quote: ... #ACS-1024) and 9-1 (UMN PCBC16iPS/vShiPS 9-1)25 were expanded in culture on vitronectin (VTN-N, Thermofisher Scientific, Cat. #A14700) in Essential 8 Medium (E8 ...
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bioRxiv - Neuroscience 2019Quote: ... HPLC-purified dipeptide repeats were purchased from Pepscan and were either labelled with a single Cy5 dye at the N-terminus during synthesis or were labelled non-site-specifically in-house using Alexa488-Fluor® Protein Labeling Kit (Thermo Fisher Scientific) using published methods (94).
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bioRxiv - Plant Biology 2020Quote: ... the samples were centrifuged before receiving 50 µL of N-Methyl-M (trimethylsilyl) trifluoroacetamide (MSTFA) + 1% trimethylchlorosilane (TMCS) (ThermoFisher Scientific, Waltham, MA, USA), briefly vortexed and incubated at 60 °C for 40 min ...
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bioRxiv - Plant Biology 2020Quote: The coding DNA sequence for human Factor IX without the native signal peptide was synthesized as N-terminal translational fusion to the signal-peptide coding sequence of PpAP1 (Pp3c5_19520V3.1)42 by GeneArt (Thermo Fisher Scientific, Waltham, MA, USA). It was cloned into the pAct5-MFHR1 plasmid41 to generate the final FIX expression vector ...
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bioRxiv - Immunology 2021Quote: ... A tPA signal was added at the N-terminus to facilitate protein secretion and the construct was cloned into pcDNA3.4 vector (Thermo Fisher, Waltham, MA, USA) which drives transcription via a CMV promoter ...
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bioRxiv - Systems Biology 2021Quote: ... the samples were centrifuged before receiving 50 μL of N-Methyl-M (trimethylsilyl) trifluoroacetamide (MSTFA) + 1 % trimethylchlorosilane (TMCS) (ThermoFisher Scientific, Waltham, MA, USA), briefly vortexed and incubated at 60 °C for 40 min ...
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bioRxiv - Cell Biology 2021Quote: ... coverslips were washed once with PBS and before mounting on objective glass with 6% n-propyl gallate diluted in glycerol and 10x PBS and combined with ShandonTM Immuno-MountTM (Thermo Scientific, cat# 9990402) in a 1:12 ratio ...
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bioRxiv - Biochemistry 2022Quote: ... was cloned into the pFastBac1 vector with a 10xHis tag appended at the N terminus and was expressed in Sf9 cells (Gibco, catalog number 12659017). The cells were cultured at 27 °C for 60 h after being infected with baculoviruses using the Bac-to-Bac system (Life Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for an additional two hours with Alexa 594 streptavidin-conjugated antibody (1:500, #S32356, RRID: N/A, Thermo fisher Scientific, USA). Sections were imaged after rinsing ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 N and goblet cells were co-stained using rabbit anti-SARS-CoV-2 Nucleocapsid monoclonal antibody (HL453) (Invitrogen, cat# MA5-36272) (1:100 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were targeted in a second bioreactor sampling experiment (n=2 for both bioreactors) for which the Plasmid Miniprep kit was used according to manufacturer’s instruction (Thermo Fisher Scientific, Waltham, US), with the addition of a step homogenizing the cells before processing ...
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bioRxiv - Cell Biology 2019Quote: ... This exogenous N-terminal GFP fusion was made by first cloning TtPOC5 into the pENTR4 Dual Selection Entry Vector (Invitrogen, Carlsbad, CA, USA) and then using the Gateway cloning system (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated at 4°C overnight with the primary antibodies (Mouse anti-Dyrk1a: Abnova, Cat. N. H00001859-M01; Rabbit anti-Camk2a: Molecular probes, PA5-14315). After washing ...
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bioRxiv - Plant Biology 2021Quote: ... A 0.5 g sample of the collected tissues were ground under liquid nitrogen and suspended in 1 ml of co-IP extraction buffer (1× PBS, 1% n-dodecyl-β-d-maltoside (Invitrogen, Carlsbad, CA), protease inhibitor cocktail cOmplete™ (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... GEMs were broken and the single strand cDNA was cleaned up using DynaBeads® MyOne™ Silane Beads (Life Technologies, P/N 37002D). cDNA was amplified using a Bio-Rad PTC-200 Thermal cycler with 0.2ml 8-strip non-Flex PCR tubes ...
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bioRxiv - Immunology 2022Quote: ... and the N-terminal peptidase domain of human ACE2 were expressed using the Bac-to-Bac Baculovirus Expression System (Invitrogen, Carlsbad, CA, USA) and purified by Strep-Tactin Sepharose (IBA Lifesciences ...
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bioRxiv - Cancer Biology 2022Quote: ... cells (n = 500 000 cells/well) were transfected with 25 nM of each siRNA individually using Lipofectamine® 3000 Transfection Reagent (ThermoFisher Scientific, # L3000075) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The distillates from the KCl extracts were quantified by titration and then acidified and oven-dried at 60 °C for N isotope analysis by mass spectrometry (Finnigan MAT251, Thermo Fisher, Waltham, MA) according to Hauck et al ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were either Nissl-stained with cresyl violet or stained with the fluorescent Nissl reagent NeuroTrace Blue (1:200) (Life Technologies, N-21479). In four subjects ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was incubated 2h at 37°C and then loaded on a 15% acrylamide/8M urea precast gel (Invitrogen, cat n° EC6885BOX). The ligated RNA was purified through gel extraction ...
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bioRxiv - Molecular Biology 2020Quote: ... The second strand cDNA was generated by performing one PCR cycle using Platinum II Hot start Taq Polymerase (Thermo Fisher, cat n°14966001). The reaction included 20 μLsingle-strand cDNA ...
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bioRxiv - Microbiology 2021Quote: ... PM-WCPV-S) or N peptide pool (Meltenyi, 130-126-698) in the presence of 1 µg/mL anti-CD28 (Invitrogen, 14-0281-86) for co-stimulation for 6 hours ...
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bioRxiv - Biochemistry 2020Quote: ... cDNA was generated using 500 ng of TotalRNA and Applied Biosystems High Capacity cDNA with Reverse Transcription kit (Applied Biosystems p/n 4368814). cDNA ...
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bioRxiv - Biochemistry 2021Quote: ... NiV RdRp P/L complex was purified using Ni-NTA affinity chromatography based on the P protein N-terminal eight-histidine tag according to the manufacturer’s specifications (Thermo Scientific, Rockford, IL, USA). The protein identities of the purified HCV nsp5b RdRp and NiV RdRp P/L complex were confirmed by mass spectrometry (MS ...
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bioRxiv - Microbiology 2021Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify N gene of SARS-CoV-2 using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described [41] using the following primers and probes ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then cross-linked at 120J/m2 and then labelled RNA was detected using the Chemiluminescent Nucleic Acid Detection module kit (Thermofisher, cat n°: 89880) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Chemical probes used in this work include alkDADA (Liechti et al., 2014), N-AlkTMM and O-AlkTMM (Foley et al., 2016) and FM4-64 FX (Invitrogen, Carlsbad, CA USA). AlkDADA was synthesized by the Einstein Chemical Biology Core and the TMM probes were kind gifts of Dr ...
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bioRxiv - Biophysics 2022Quote: ... The terminal free amine on the spacer was reacted with the N-hydroxysuccinimide (NHS) ester group of sulfo-SMCC (Thermo Fisher Scientific, 22622). The maleimide group of sulfo-SMCC was subsequently conjugated to 3’ thiol modified DNA oligonucleotides as described19 ...
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bioRxiv - Molecular Biology 2024Quote: ... 2008) N-terminally tagged with the V5 epitope was synthesized as a DNA string (GeneArt Strings DNA Fragments, Thermo Fisher Scientific, Schwerte, Germany) and cloned into pcDNA3.1 to obtain pcDNA3.1-V5-RHEB-Q64L ...
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bioRxiv - Neuroscience 2024Quote: ... Neuronal differentiation was induced by culturing NGN2-NPC in neural differentiation medium (DMEM/F-12 + Glutamax, Gibco®; 1x N-2 supplement, Gibco®; 1x B27 supplement without vitamin A, Gibco®) supplemented with doxycycline (2µg/ml) ...
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bioRxiv - Biochemistry 2024Quote: ... or 5 × 103 cells/mL (N. gruberi) in 100 µL of the indicated medium in white TC-treated 96-well plates (Thermo Scientific Nunc Microwell 136101). For assays under standard growth conditions (“+ glc”) ...
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bioRxiv - Microbiology 2023Quote: ... Total cDNA was obtained by reverse transcription using random hexamers pd(N)6 and M-MLV reverse transcriptase (Life Technologies, Carlsbad, CA, USA) at 42 °C for 50min ...
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bioRxiv - Immunology 2023Quote: Open-reading frames coding for either Nefmut alone or fused with SARS-CoV-2 N protein were cloned into pVAX1 plasmid (Thermo Fisher, Waltham, MA) as previously described [20] ...
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bioRxiv - Biochemistry 2023Quote: The RBD protein was incubated for 30 min at 21 °C and 750 rpm with a 10 molar equivalent of sulfo-m-maleimidobenzoyl-N-hydoxysuccinimide ester (Sulfo-MBS) (Thermo Scientific; MW 416.30). The excess crosslinker was removed by passing the protein through a PD-10 column using a PBS ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were 1:30 rat anti-N-cadherin (Developmental Studies Hybridoma Bank, DN-EX #8,) and 1:200 rabbit anti-lucifer yellow (Molecular Probes, A-5750). The secondary antibodies used were 1:500 goat anti-rabbit with Alexa fluor 633 (Molecular Probes ...
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bioRxiv - Microbiology 2023Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify N gene of SARS- CoV-2 using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described (73 ...
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bioRxiv - Biochemistry 2023Quote: ... n=4) collected in microcentrifuge tubes were subjected to a 30-min incubation in 30 μM iodoacetamide (IAM) (Thermo Fisher Scientific, Waltham, MA) or N-ethylmaleimide (NEM ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: MARC-145 cells transfected with the recombinant pCAGGS-N plasmid were lysed in Pierce™ IP Lysis Buffer (Thermo Fisher Scientific, Waltham, MA), and then mixed with each of the purified anti-N mAbs ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-N-WASP (Cell Signalling #4848S; 1:100) followed by Alexa Fluor 568 conjugated secondary antibodies (Invitrogen; 1:1000 in blocking buffer). Actin tails were labeled with Alexa Fluor 488 phalloidin (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... The peptides were separated at a flow rate of 300 nL/min on C18 pre-column (Acclaim PepMap™ 100, 75 µm × 2 cm, nanoViper, P/N 164946, ThermoFisher Scientific Incorporation) followed by analytical column (Acclaim PepMap™ RSLC C18 ...