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Citations for Thermo Fisher :
9701 - 9750 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... HepG2 cells (4×104 /well) were seeded in a 96-well plate and cultured overnight in DMEM (Invitrogen) supplemented with 10% heat-inactivated FCS ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured in six-well plates coated with recombinant human vitronectin (Life Technologies, catalogue no. A14700) using Essential 8 medium (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... 38 ml of this medium was poured into an Nunc OmniTray single-well plate (Thermo Fisher Scientific, 242811) and left to dry under a laminar flow hood for 15 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... The plates (384 Well Optical Btw Plt Polybase Black w/o Lid Non-Treated PS, Thermo Fisher Scientific) were incubated at 37□°C with cycles of 9□min shaking (700□rpm double orbital ...
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bioRxiv - Neuroscience 2024Quote: ... the plate was placed in the 7900HT Fast Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific, USA) qPCR machine to evaluate the gene expression ...
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bioRxiv - Neuroscience 2024Quote: Primary hMDM were plated at 15,000 cells/well in a 96-well black-walled plate (NUNC, ThermoFisher Scientific) and treated with a range of concentrations of dopamine (1×10-10M to 5×10-4M ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the GeneJET Gel Extraction Kit (ThermoFisherScientific, Vilnius, Lithuania) and quantified using a fluorometric RiboGreen kit (Qubit Assay Kit, Invitrogen, Life Technologies Corporation, Oregon, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM EDTA pH 8.0 and then labeled with the ULYSIS™ Alexa Fluor™ 594 nucleic acid labeling kit (Thermo Fisher Scientific, MA, USA). Two μg of either an equimolar mixture of the eleven Altivir_1_MSI polynucleotides or of the single negative control polynucleotide were used in a single labeling reaction and then purified using NucAway Spin Columns (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Complementary DNA was synthesized from 1 μg total RNA using the Thermo Scientific™ Maxima™ First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Scientific, cat. #K1641) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were then run on a 1% agarose gel and appropriate bands were cut and gel-extracted using a GeneJET Gel Extraction Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Cleaved Tn5 was eluted in ~1 mL fractions and Tn5 elution efficiency was monitored by the Pierce™ Coomassie (Bradford) Protein Assay Kit (Thermo Fisher Scientific cat# 23200). Fractions with the strongest absorbance were pooled and dialyzed versus two changes of 2 L of 2X Tn5 dialysis buffer (100 mM HEPES-KOH at pH 7.2 ...
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bioRxiv - Cancer Biology 2019Quote: ... and 1-2 μg of total RNA was used for synthesizing cDNA with High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA, USA). cDNA was then used for Q-PCR in a StepOnePlusTM (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions were then run on a 1% agarose gel and appropriate bands were cut and gel-extracted using a GeneJET Gel Extraction Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... the amplicons were gel-verified (2-µl aliquots were run on a 1% agarose gel) and column-purified (GeneJET PCR purification Kit, Thermo Fisher Scientific, catalog no. K0701). For cloning into ArEc-TDH3 ...
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bioRxiv - Microbiology 2020Quote: ... all end-point rpoB and 16S gene amplicons were pooled at equimolar concentrations (∼1-2 ng/µl) using a SequalPrep normalization kit (Thermo Fisher Scientific, Waltham, MA, USA) and purified by gel extraction using the Wizard SV gel and PCR clean-up system (Promega ...
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bioRxiv - Systems Biology 2020Quote: ... The quantitative RT-PCR reactions were done using Applied Biosystems™ TaqMan™ RNA-to-CT™ 1-Step Kit and Custom TaqMan Gene Expression Assays (Thermo Fisher Scientific). The standard curve method was used to determine absolute mRNA concentrations for the samples ...
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bioRxiv - Cancer Biology 2020Quote: In order to identify activated T cells up to 1 million of thawed PBMCs were washed twice in PBS and stained with the viability dye LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (ThermoFisher, Cat no. L34966, 1:100). Then ...
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Telomerase deficiency in humans is associated with systemic age-related changes in energy metabolismbioRxiv - Cell Biology 2022Quote: ... 1ug of DNAse 1 treated RNA was reverse-transcribed into first-strand cDNA using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems; Thermo Fisher Scientific, Inc.). The primer sequences and thermal cycling parameters used to quantify fully spliced hTERT (primer set E4-E5 ...
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bioRxiv - Genomics 2022Quote: We diluted the RNA samples to 1 ng/μL for library preparation and confirmed the concentration using a Qubit RNA High Sensitivity Assay Kit (Invitrogen™, Thermo Fisher Scientific, Waltham, MA) and Qubit 4 Fluorometer (Invitrogen™ ...
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bioRxiv - Microbiology 2022Quote: ... AMPure XP beads at a ratio of 0.8:1 to remove primer dimers and a final cleanup using the Purelink PCR Purification Kit (Life Technologies Cat #K310001; Grand Island, NY). The purified pool was quantified in triplicate with a Qubit fluorimeter prior to sequencing.
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bioRxiv - Bioengineering 2022Quote: Measurements of COD on filtered mixed liquor were performed using Hach Lange test kits LCK114 (Fisher Scientific; measurement range 150–1000 mg COD L-1) in the first batch experiment ...
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bioRxiv - Immunology 2019Quote: The concentration of sPD-1 and sTim-3 was measured in thawed plasma samples by enzyme linked immunosorbent assay (ELISA) using Human PD-1 (PDCD1) ELISA kit [EHPDCD1] (Thermo Fisher Scientific, Waltham, MA USA) and Quantikine ELISA Human TIM-3 Immunoassay kit [DTIM30] (R&D Systems ...
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bioRxiv - Pathology 2020Quote: ... approximately 50 ng of RNA was used in a previously published qRT-PCR duplex assay (Price et al. 2010) with the TaqMan® RNA-to-Ct™ 1-step kit (Applied Biosystems™, ThermoFisher Scientific) on a QuantStudio™ 3 Real-Time PCR system (Applied Biosystems™ ...
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bioRxiv - Immunology 2021Quote: ... a 1 mg/mL solution of these proteins was mixed with the Protein Thermal Shift™ Dye kit (Thermo Fisher, Waltham, MA, USA; Cat # 4461146) in triplicate as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μg of total RNA per reaction was used to synthesize cDNA with the SuperScript® VILO™ cDNA Synthesis Kit (Invitrogen, Thermo Fisher Scientific). Quantitative PCR was performed with the Applied BiosystemsTM PowerUP™ SYBR™ Green Master Mix from Applied Biosystems on a real-time PCR instrument (LightCycler® 480 System ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA samples (up to 1 μg) were reverse transcribed using the oligo(dT) primer from the High Capacity cDNA Reversion Transcription Kit (Thermo Fisher Scientific, Waltham, MA, USA). mRNA expression of the TAM receptors Tyro-3 (Tyro-3) ...
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bioRxiv - Microbiology 2021Quote: ... The extracted DNA yield (ng DNA g-1 dry weight soil) was quantified with the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). We recognize that this calculation does not take into account inflation in DNA yield due to the growth of fungi and other organisms not accounted for in our study ...
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bioRxiv - Immunology 2021Quote: ... In order to assess proliferation T cells were resuspended at 1 × 106 cells/ml using a 2 μM solution of CellTrace™ Violet Cell Proliferation Kit (Thermo Fisher Scientific, Massachusetts, USA) in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... the isolated viral RNA was tested by one-step RT-PCR using the Verso 1-Step RT-PCR Kit (Hot Start) (Thermo Fisher Scientific, Waltham, MA, USA) and nested primers—MarBox1F and MarBox1R ...
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bioRxiv - Molecular Biology 2022Quote: ... were amplified by RT-PCR using the Verso 1-Step RT-PCR Kit with Thermo-Start Taq (Hot Start; Thermo Fisher Scientific, Waltham, MA, USA) using primers Mar- CPL-F and MarCP2R (876 bp ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA synthesis and PCR analysis was carried out from equal amount of total RNA (1 μg) from each sample utilizing One-Step kit (Invitrogen Life Technologies, Carlsbad, CA, USA). Primers for target gene were designed using OligoAnalyzer 3.1 software ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed with PBS and stained for 15 min at RT with fixable Near-IR Dead Cell Staining Kit (Thermofisher L10119, 1/1000 in PBS). After three washes ...
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bioRxiv - Microbiology 2022Quote: ... Cell-associated HIV RNA was measured using the same primers in a one-step qPCR using TaqMan® RNA to Ct™ 1 Step kit (Applied Biosystems, catalog # 4392656). Cell counts were determined by qPCR using human RPLP0 (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... labeled peptides on one spin tip from a High-Select™ TiO2 Phosphopeptide Enrichment Kit (capacity of 1–3 mg; Thermo Fisher Scientific, catalog A32993). After preparing spin tips ...
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bioRxiv - Systems Biology 2022Quote: ... Quality check was followed by reverse transcription of 1 μg total RNA per reaction using High- Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA), according to the manufacturer’s manual ...
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bioRxiv - Evolutionary Biology 2024Quote: We collected three replicates of 15-20 larvae for each day of development (days 1-4) and extracted total RNA using the PureLink RNA Mini Kit (Thermo Fisher Scientific; Cat no. 12183018A) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... DNA Fragments were subsequently cloned into a PCR-linearized pZE21 expression vector (primers 1 and 2) through blunt-ended ligation (Thermo Fisher Rapid DNA Ligation kit). Ligated products were run on a 0.5% agarose gel to isolate fragments between 5 and 10 kb ...
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bioRxiv - Molecular Biology 2023Quote: ... Hybridization of the fragmented RNA (100 ng) was performed with a barcode from the Xpress RNA-Seq Barcode 1-16 Kit (Thermo Fisher Scientific, Waltham, MA, USA), Ion Adaptor Mix ...
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bioRxiv - Developmental Biology 2023Quote: ... A total of 1 μg of RNA per reaction was reverse-transcribed using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Thermo Fisher Scientific, 4368814). The following primers were used:
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bioRxiv - Genetics 2024Quote: ... The DNA-bound beads were then incubated with 60 μg nuclear extract from BL2 cell line or PBMC or no nuclear extract for 1 h at room temperature in LightShift Chemiluminescent EMSA Kit reaction buffer (Thermo Fisher Scientific, catalog number 20148X). After washing and separation ...
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bioRxiv - Immunology 2021Quote: ... IL-2 Human Uncoated ELISA kit and IFNγ Human Uncoated ELISA kit (both from Invitrogen) and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA was synthesized using a commercial kit (High-Capacity RNA-to-cDNA kit, Thermo Scientific). Real-time qPCR (7500 Real-Time PCR System ...
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bioRxiv - Biochemistry 2021Quote: ... and quantified using a commercially available BCA kit (Pierce™ BCA Protein Assay Kit, ThermoFisher) according to the manufacturer instructions ...
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bioRxiv - Plant Biology 2021Quote: RNA was isolated using PicoPure kits (Arcturus PicoPure RNA isolation kit, ThermoFisher Scientific; MA, US), with additional on-column DNAse digestion (RNase-free DNase ...
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bioRxiv - Developmental Biology 2022Quote: ... EdU tracing was done with the kit reaction components according to kit instructions (C10337, Invitrogen). Sections were then incubated again in 3% BSA/PBS for 10 minutes ...
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bioRxiv - Pathology 2022Quote: ... Aβ42 human ultrasensitive ELISA Kit and Tau (phospho) [pT231] human ELISA Kit from Thermo Fisher, and sAPPα and sAPPβ ELISA Kit from Mybiosource ...
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bioRxiv - Microbiology 2022Quote: The Pierce High pH Reversed-Phase Peptide Fractionation Kit (kit no.: 84868; Thermo Fisher Scientific) was used to fractionate 150 μg of triple dimethyl-labeled and mixed proteome ...
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bioRxiv - Developmental Biology 2020Quote: ... Staining was performed with the EdU Click-It kit (Alexa Fluor 555 kit; ThermoFisher, #C10338) following the manufacturer’s protocol (BSA not added) ...
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bioRxiv - Immunology 2021Quote: ... and quantified using a fluorometric kit (Quant-iT PicoGreen dsDNA Assay Kit; Invitrogen, Cat#P7589). Purified amplicons were pooled in equimolar concentrations with a SequalPrep Normalization Plate Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... DNA was quantified using the Quant-iT dsDNA Assay kit high sensitivity kit (ThermoFisher, USA). Microbial community composition was determined by Illumina sequencing of the V4 region of the 16S rRNA gene followed by analysis using QIIME 2 2019.7 (Bolyen et al. ...