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Citations for Thermo Fisher :
9651 - 9700 of 10000+ citations for Mouse Receptor Interacting Serine threonine Protein Kinase 1 RIPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and rat anti-glial fibrillary acidic protein (GFAP) Mab (ThermoFisher Scientific #13-0300, 1:500). Sections were then washed in PBST and incubated with fluorophore-conjugated secondary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... AEBSF 1 mM) with 2 µg of antibodies and Dynabeads Protein G (Thermo Fisher Scientific). Control immunoprecipitation (IP ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM EDTA) and loaded onto magnetic beads (dynabeads, protein G, 2.8 μm diameter, Invitrogen), which were previously decorated with sub-saturating quantities of a monoclonal anti-FLAG antibody (M2 ...
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bioRxiv - Neuroscience 2023Quote: ... the remaining lysate precleared for 1 hour with Protein-A Dynabeads (Thermo Scientific, no. 10001D). FLAG-M2 antibody Protein-A Dynabead conjugate was incubated with the precleared lysate overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... supplemented with 1% (v/v) PFHM-II Protein-Free Hybridoma Medium (Thermo Fisher Scientific, # 11370882), hereafter referred to as APEL™2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mg lysate was pre-cleared with Dynabeads™ protein G (cat# 10004D, Thermo Fisher) for 1 h with rotation at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... then 1 hour with Pierce™ Protein A/G agarose beads (Thermo Fisher Scientific, 20421). Beads were washed 4 times with 0.1% NP-40 substitute (VWR ...
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bioRxiv - Molecular Biology 2023Quote: ... The system was calibrated with NativeMark Unstained Protein Standard (Invitrogen, Cat. #LC0725, 1:400 dilution). Buffer 1 was measured on each day prior to experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... Actin was used as the protein loading control (1:5000; MA5-15739, Thermo Fisher Scientific). Immunoreactive bands were detected with horseradish peroxidase-coupled anti-mouse or anti-rabbit antibodies (CST ...
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bioRxiv - Neuroscience 2023Quote: ... Rabbit anti-GAPDH was used as housekeeping reference protein antibody (Invitrogen PA1-987; 1:1000). The secondary antibodies were horseradish peroxidase (HPR)-conjugated (Sigma-Aldrich A0545 ...
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bioRxiv - Cancer Biology 2023Quote: ... Blots were probed with antibodies against the following proteins: sGCα1 (1:2,000, ThermoFisher MA5-17086), sGCβ1 (1:2,000 ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies used for protein visualization were the following: rabbit AF488 (1:1000, Invitrogen), guinea pig Cy3 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... 500 μg precleared cell protein lysate was treated with 1 U RNase I (Ambion AM2294) for 5 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1-20 µg of protein was loaded onto 4-12% NuPage BisTris gels (Invitrogen) for electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: Viral injections were validated using anti-GFP (anti-Green Fluorescent Protein, 1:500, Invitrogen, #11122) as previously described 17 ...
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bioRxiv - Biochemistry 2022Quote: ... Constructs for wild-type receptors were cloned into MIG by Gibson assembly as described above with the BstBI (Fisher Scientific 50-812-170) and NotI (Fisher Scientific 50-811-2040 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were collected from the flask, centrifuged (10 min, 150g, RT) and blocked for Fc receptors by using antibody CD16/32 (Invitrogen, 14-0161-82) (1:100) ...
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bioRxiv - Pathology 2021Quote: ... Detailed immunohistochemistry staining and quantification procedures for each marker have been published (6, 16–26) or are in preparation for estrogen receptor alpha (antibody SP1; Thermo Scientific, Waltham, MA) and an antibody (PPG5/10 ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBDmut) (K417N, E484K, N501Y) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBD417, RBD501, RBD484 and RBDtrip) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Microbiology 2024Quote: ... After lysis of red blood cells with Gibco ACK lysing buffer and blocking of Fc receptors (Invitrogen CD16/CD32 clone 93, RRID AB_467133), cells were stained with live/dead aqua (Thermo ...
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bioRxiv - Bioengineering 2023Quote: ... we respectively used Ethidium Homodimer-1 (EthD-1; LIVE/DEAD Viability/Cytotoxicity Kit, Invitrogen) and 4kDa fluorescein Isothiocyanate-Dextran (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... and phosphatase/protease inhibitors in water) and addition of a 1:1 ratio of protein A and G magnetic beads (Life Technologies) were used to bind crosslinked protein/DNA ...
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bioRxiv - Immunology 2021Quote: ... The bottom chamber of the plate contained PBS with 20 ng/well monocyte chemoattractant protein-1 (MCP-1, Thermofisher Scientific, USA). Macrophages (5×104 cells in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... 1 mg of striatal lysates was pre-cleared for 1 h with 80 ul Protein G Sepharose coated beads (ThermoFisher Scientific), then centrifuged at 4°C for 5 min at 1000 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... 10 pmol each) were mixed with spCas9 protein (IDT) at 1:1 ratio and transfected into 100K C2C12 cells by electroporation (Thermofisher Neon) in the following parameters ...
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bioRxiv - Biochemistry 2022Quote: The purified AAGAB protein was cross-linked with a solution of 1:1 BS3-d0: BS3-d4 (ThermoFisher #21590 and #21595) crosslinkers ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 37°C to OD600 0.6 and protein expression induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG; ThermoFisher Scientific) for 4 hr at 30°C (3CL-pro and RBD) ...
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bioRxiv - Microbiology 2021Quote: ... Labelled proteins were stained for 1 hour at room temperature using Alexa Fluor 488/594-conjugated goat antibodies (1:2000, Life Technologies). Nuclei were stained using the intercalating DNA dye DAPI at 5 μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... Samples with equal amounts of protein were mixed with a 1:1 mixture of 4x NuPAGE LDS Sample Buffer (Invitrogen, #NP0007) and 500 mM DTT (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred to a nitrocellulose membrane and blocked with a 1:1 dilution of SEA BLOCK (Thermo Fisher, Waltham, Massachusetts):PBS overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... or a major outer membrane protein (MOMP) antibody (1:1000, Novus Bio) and an appropriate AlexaFluor secondary antibody (1:200, ThermoFisher Scientific). Counterstaining was performed with Hoechst stain ...
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bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-IgG 1:500, rabbit anti-Dp 212 1:500 (Dimova et al, 2003) and Dynabeads protein G (Invitrogen # 10003D). Chromatin precipitate was eluted from Dynabeads with elution buffer 1 (10mM EDTA ...
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bioRxiv - Microbiology 2024Quote: ... The samples were mixed with 30 μl ChIP-grade protein A/G plus agarose beads (1: 1 slurry, ThermoFisher Scientific 26159) for 1 hour at 4°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Pellets were resuspended with 20 mL Lysis buffer containing a 1:1 mixture of B-PER Protein Extraction Reagent (Thermo Scientific): 50 mM Tris-HCl buffer ...
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bioRxiv - Bioengineering 2023Quote: ... The barrier function of the Calu-3 monolayer was terminally evaluated via immunofluorescence imaging of tight junction protein 1 (ZO-1 antibody, Invitrogen, 339194) and permeability assays using 500 µL of 2 mg/mL FITC-dextran (4 kDa ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were bound to 10 μl prewashed beads (1:1 mix of hydrophilic and hydrophobic Cytiva SeraMag beads, Fisher Scientific), and the samples were diluted to 50% acetonitrile with 55 μl acetonitrile ...
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bioRxiv - Molecular Biology 2023Quote: ... or anti-NIPBL rabbit antibodies followed by 1 h incubation at 4 °C on a rotating wheel with 40 μl of a 1:1 mix of Dynabead protein A (10001D, Life Technologies) and G (10003D ...
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bioRxiv - Biochemistry 2023Quote: ... containing 1 mM EDTA and 1 mM dithiothreitol were reacted with a 4-fold concentration of Protein Thermal Shift Dye (Life Technologies) in a 20 μl reaction volume ...
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bioRxiv - Genetics 2024Quote: ... Then chromatin was pre-cleared with 10 μl of 1:1 protein A:protein G Dynabeads (Thermo Fisher Scientific, 10001D and 10003D). The precleared chromatin sample was incubated with 0.5 μg of anti-H3K4me3 (Millipore 17-614 ...
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bioRxiv - Genomics 2023Quote: The MNase digested chromatin solution of each sample was pre-cleared with 100μl Dynabeads Protein A/G 1:1 mix magnetic beads (Invitrogen 10001D, 10003D) and rotated at 4℃ for 2h ...
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bioRxiv - Immunology 2024Quote: ... or LAMP-1/CD107a monoclonal antibody specific for the lysosomal-associated membrane protein 1 (LAMP-1) of the parasitophorous vacuole (rat IgG2a FITC, Invitrogen, CA). Revelation was performed with 1.5 μg/ml streptavidin conjugated to Texas Red (Molecular Probes ...
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bioRxiv - Systems Biology 2019Quote: ... Alexa Fluor 488-conjugated goat anti-rabbit IgG and Alexa Fluor 647-conjugated goat anti-mouse IgG diluted in washing solution for 1 h (1:100, Thermo Fisher Scientific Inc.; A11008 and 1:500, Thermo Fisher Scientific Inc.; A21236). After washing ...
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bioRxiv - Cancer Biology 2020Quote: ... and anti-NF200 has been conjugated to AF647 using the AlexaFluor® 647 protein labeling kit (Molecular probes).
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bioRxiv - Cell Biology 2022Quote: GeneArt-CRISPR/Cas9 Nuclease vector with OFP (Orange Fluorescence Protein) kit (A21174) was from Life Technologies (Invitrogen, USA). We used http://crispr.mit.edu to design guided RNAs and http://www.rgenome.net/cas-offinder/ (RGEN tools ...
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bioRxiv - Neuroscience 2021Quote: ... iPSC-derived neurons and iPSC-derived astrocytes using the MEM-PER Plus Membrane Protein Extraction Kit (ThermoFisher Scientific), and protein concentrations were determined by bicinchoninic acid (BCA ...
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bioRxiv - Genomics 2020Quote: ... The concentration of the nuclear extracts was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), before extracts were aliquoted and stored at −70°C to be used for western blotting ...
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bioRxiv - Microbiology 2019Quote: ... cells were washed with PBS and fractionated using a subcellular protein fractionation kit for cultured cells (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Protein extracts were cleared by centrifugation and purified with Pierce™ GST Spin Purification Kit (ThermoFisher Scientific, #16106). Western analysis was performed using standard procedures and used antibodies and concentrations are presented in Table S2.
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bioRxiv - Immunology 2021Quote: Membrane proteins from ZIKV-infected or uninfected JEG-3 were isolated by Mem-PER Plus Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...