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Citations for Thermo Fisher :
9601 - 9650 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was incubated with Dynabeads Protein A (Thermo Fisher Scientific), previously coupled with anti-TcSOD-A or anti-TcSir2rp3 antibodies ...
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bioRxiv - Developmental Biology 2020Quote: ... Antibody was pre-bound by incubating with Protein-G Dynabeads (Invitrogen 10004D) in blocking buffer (PBS supplemented with 0.5% TWEEN and 0.5% BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... After the addition of 50 µL Dynabeads protein-A (Life Technologies, 10002A), the mixture was incubated at 4 °C for another 2 h ...
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bioRxiv - Developmental Biology 2020Quote: ... then proteins denatured by addition of 4x Laemmli sample buffer (Thermo Fisher) and heating at 90°C for 5 minutes.
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bioRxiv - Genomics 2020Quote: ... Sheared chromatin was precleared with 20 μl Dynabeads Protein A (Life Technologies) at 4 °C for 1 hr.
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bioRxiv - Genetics 2022Quote: ... The supernatants were incubated with 30 μl Dynabeads Protein A (Thermo Fisher) preloaded with 1.2 μl anti-FLAG M2 antibody (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... His-tagged proteins were purified using HisPur Ni-NTA Resin (Thermo Fisher). Resin-bound proteins were washed (25 mM Imidazole ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentration was determined using a microplate and BCA assay kit (Thermofisher) as per manufacturer’s specifications ...
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bioRxiv - Biophysics 2022Quote: The concentration of purified proteins was determined using NanoDrop One (Thermo Scientific). For performing SDS-PAGE ...
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bioRxiv - Biochemistry 2022Quote: Protein lysates were prepared in RIPA buffer and BCA Assay (Thermo Scientific) was used to determine protein concentration ...
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bioRxiv - Neuroscience 2021Quote: ... and protein concentrations were determined by bicinchoninic acid (BCA) assay (ThermoFisher Scientific). For western blotting ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclear proteins were isolated using the USB Nuclear Extraction Kit (Affymetrix, Inc.) and the Procarta Transcription Factor Assay (Affymetrix ...
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bioRxiv - Neuroscience 2021Quote: ... Following quantification using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), equal amounts of total protein were loaded onto for each sample for standard western blot.
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bioRxiv - Molecular Biology 2021Quote: ... Nuclear and cytoplasmic fractions were quantified by BCA Protein Assay kit (Thermofisher).
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bioRxiv - Molecular Biology 2021Quote: ... the lysates were incubated with 10 μL of Dynabeads Protein G (Invitrogen) for 1 hr at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 50ul (1.5mg) magnetic beads (Dynabeads Protein G Immunoprecipitation kit, Invitrogen, Cat: 10007D) were prepared per RIP ...
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bioRxiv - Molecular Biology 2020Quote: ... immunocomplexes were precipitated by incubation with pre-washed Protein A Dynabeads (Invitrogen) for 2h ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was measured using the BCA assay (Thermo Scientific, Massachusetts, USA). Reducing sample buffer was added ...
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bioRxiv - Genomics 2020Quote: ... was mixed with 30 µl of Protein A Dynabeads (Invitrogen, cat. # 1002D) and incubated in blocking solution (0.5% bovine serum albumin in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... and fractionated on NuPAGE 4-12% Bis-Tris Protein Gels (ThermoFisher Scientific), followed by the transferring of resolved proteins onto PVDF membranes via an iBlot 2 Dry Blotting System (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... resolved on NuPAGE 4-12% Bis-Tris protein gels (Thermo Fisher, NP0323BOX), and transferred to 0.2 µm PVDF membranes (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated on NuPAGE 4-12% Bis-Tris gels (Life technologies) before transferring proteins onto Nitrocellulose membranes and incubating with anti-BiP (C50B12 ...
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bioRxiv - Molecular Biology 2021Quote: ... Induced proteins were purified on HisPur Ni-NTA Superflow Agarose (ThermoFisher 25214) and eluted with elution buffer containing 150 mM Imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were identified and quantified using Proteome Discoverer 2.2 (Thermo Fisher Scientific) with MASCOT program ver 2.6 (Matrix science ...
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bioRxiv - Molecular Biology 2020Quote: ... 1x protease inhibitor cocktail) and incubated with Protein-G Dynabeads (Thermo Fisher) for 2 hours at 4 °C for pre-clearing ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein samples were resolved in 4-12% NuPAGE Bis-Tris gels (ThermoFisher), stained with colloidal Coomassie Brilliant Blue and stored in sealed packages until mass spectrometry analysis.
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bioRxiv - Molecular Biology 2019Quote: ... SDS PAGE was stained by Imperial Protein Stain (Thermo Scientific, cat#24615), and the gel between 43 kDa to 55 kDa were cut ...
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bioRxiv - Cell Biology 2022Quote: ... 48 well plates were coated with 50 µg/ml protein G (Invitrogen) overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were detected using SuperSignal West Pico Plus Chemiluminescent Substrate (ThermoFisher Scientific) and quantitated using an Amersham Imager 600 (GE Healthcare Life Sciences).
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bioRxiv - Cell Biology 2022Quote: ... followed by addition of 50 μl of Protein A agarose beads (Invitrogen) pre-equilibrated in the lysis buffer and additional incubation for 1 h at room temperature on a rocker ...
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bioRxiv - Developmental Biology 2022Quote: ... The protein content was quantified using a bicinchoninic acid assay (ThermoFisher Scientific), normalized to 10 μg ...
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bioRxiv - Immunology 2022Quote: Proteins from cells or tissues were prepared using RIPA buffer (ThermoFisher Scientific) containing protease and phosphatase inhibitors ...
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bioRxiv - Genomics 2020Quote: ... the SDS-PAGE-separated proteins were transferred to a PVDF membrane (ThermoFisher). GFP was detected using an anti-GFP antibody (11814460001 ...
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bioRxiv - Biochemistry 2020Quote: ... Biotinylated proteins were enriched with Pierce™ streptavidin bead (Thermo Scientific™). The protein pellets were mixed to a pre-washed streptavidin beads (100 mM Hepes buffer) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were separated using 12% Bis-Tris Acetate gels (Invitrogen, Carlsbad, CA) with MOPS buffer (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... Protein concentrations were determined with the BCA kit (Thermo Fisher, Waltham, MA). Oligonucleotides to be used as probes were synthesized with a 5’IRDye 700 modification (IDT) ...
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bioRxiv - Genomics 2020Quote: ... Antibody-bound material was isolated on Protein A-Sepharose beads (Invitrogen, UK) and DNA from antibody-bound and input chromatin was purified by PCR purification kit (Qiagen) ...
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bioRxiv - Genomics 2020Quote: ... For chromatin immunoprecipitation 10 μl protein-G magnetic beads (Pierce™ ThermoFisher) were washed in IP-buffer and incubated with 1 μg Anti-FLAG Ab (Sigma-Aldrich #F3165 ...
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bioRxiv - Genomics 2020Quote: ... Immunoprecipitation (IP) was performed with protein A-coupled magnetic beads (Invitrogen Dynal). 20 μg yH2AX antibody ...
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bioRxiv - Immunology 2021Quote: All recombinant proteins were expressed and purified using Expi293F cells (Life Technologies,) as described in detail previously [34 ...
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bioRxiv - Molecular Biology 2021Quote: ... PVDF membranes were used for protein transfer with Mini Blot Modules (Invitrogen) and 10% methanol in the transfer buffer and 5% milk in TBS-T for blocking (1 h at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... overnight followed by a 2-hour incubation with Protein G Dynabeads (Invitrogen). The beads were washed 3 times with buffer (10mM TRIS-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 μl protein-A/G magnetic beads (10002D/10004D, Thermo Fisher Scientific), 200U RNase inhibitor (N2611 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and a Qubit Protein Assay Kit (#Q33211, Thermo Fisher Scientific, MA, USA). The sfGFP purification samples were combined and analysed via sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE ...
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bioRxiv - Immunology 2019Quote: ... Protein content was measured by spectrophotometry (Nanodrop 2000, ThermoFisher Scientific, Waltham, USA) selecting Protein A280 program and 50 µg of proteins were used for trypsin digestion ...
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bioRxiv - Biochemistry 2019Quote: ... Protein concentrations were measured by the bicinchoninic assay (BCA) kit (Thermo Scientific).
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bioRxiv - Cell Biology 2019Quote: ... A Coomassie Plus (Bradford) Protein Assay kit (Thermo Fisher Scientific, MA, USA) was used to determine protein concentrations with bovine serum albumin as the standard.
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bioRxiv - Biochemistry 2020Quote: ... Proteins were separated on NuPAGE Bolt 4-12% SDS-polyacrylamide gels (Invitrogen) and subsequently scanned on a Typhoon scanner using the indicated excitation and emission modules ...
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bioRxiv - Biochemistry 2019Quote: ... The protein purity was analyzed using 4-12% SDS-PAGE (Life Technologies) and all samples used in the study were > 80 % pure ...
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bioRxiv - Microbiology 2019Quote: ... Protein concentrations were determined using a Nanodrop Lite spectrophotometer (Thermo Fisher Scientific) and equal protein amounts (typically 100 μg ...