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Citations for Thermo Fisher :
9451 - 9500 of 10000+ citations for Recombinant Human PDCD1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations of the cell and tissue lysates were determined using BCA protein assay kit (Pierce, Thermo Fisher Scientific). Lysates were subjected to SDS-PAGE and proteins were transferred onto a nitrocellulose membrane (Biorad) ...
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bioRxiv - Cell Biology 2020Quote: ... Following determination of the protein concentration in the supernatants (Pierce™ BCA Protein Assay Kit; Thermo Fisher Scientific, 23250), samples were prepared for denaturing gel electrophoresis by addition of Laemmli Sample Buffer (BioRad ...
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bioRxiv - Genetics 2021Quote: ... 10 μg of protein were loaded onto a 15-well NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen), run at 100 V for 2.5 hours ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations were quantified using 660 nm Protein Assay Reagent with Ionic Detergent Compatibility Reagent (IDCR) (Thermo Fisher Scientific). Equal amounts of protein extracts underwent vacuum filtration through a pre-wet 0.2 μm pore size nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein concentration of the RIPA-soluble fraction was measured using a BCA protein assay kit (Thermo Fisher Scientific). The protein concentration of the urea-soluble fraction was assumed to have the same ratio as the RIPA-soluble fraction ...
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bioRxiv - Developmental Biology 2021Quote: ... the sample was incubated with 300 µg protein A or protein G dynabeads (Life technologies, Cat 10001D or 10003D) for 2hrs with rotation at 4℃ ...
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bioRxiv - Physiology 2022Quote: ... The membrane was subsequently stained for total protein with Pierce™ Reversible Protein Stain Kit (Thermo Fisher, Waltham, MA). The membranes were probed with Anti-O-Linked N-Acetylglucosamine antibody (anti-RL2 ...
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bioRxiv - Cell Biology 2022Quote: ... 150 µg of protein extract from each sample were ran on NuPAGE 4%–12% Bis–Tris Protein Gels (Invitrogen) using MES running buffer (100 mM MES ...
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bioRxiv - Physiology 2022Quote: ... The total protein concentration in the lysates was determined using a Pierce BCA Protein Assay kit (Thermo Scientific, 23225). A 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels was used ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant was collected and protein concentration was measured using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, catalog #23227) and Gen5 3.04 software (BioTek) ...
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bioRxiv - Neuroscience 2021Quote: ... protein was extracted from 3-week mature neurons using neuronal protein extraction reagent (N-PER) (Fisher Scientific, Waltham, MA) and protease inhibitor cocktail (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... the antibody-protein-chromatin complex was retrieved by adding 25μl pre-washed Protein G Dynabeads (Thermo Fisher Scientific, 10004D). Immunoprecipitated chromatin DNA was de-crosslinked by 65°C heating overnight using elution buffer (1% SDS ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was evaluated and 1 mg total protein was incubated with 30 μl streptavidin-magnetic beads (ThermoFisher, 88816) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... The total amount of protein was quantitated by using Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Scientific). Equal amounts of proteins were resolved on 10 or 15% Tris-glycine SDS-PAGE under reducing conditions and transferred onto Immobilon-P Membrane ...
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bioRxiv - Cell Biology 2022Quote: Total protein was quantified for all samples using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific 23225) as per the manufacturer’s instructions.
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A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin VbioRxiv - Microbiology 2022Quote: ... Lysates were then incubated for one hour at 4°C with Dynabeads-Protein A or Dynabeads-Protein G (ThermoFisher) that had been bound to anti-GFP (clone 3E6 ...
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bioRxiv - Genomics 2020Quote: ... Total protein present in the supernatants were quantified using Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA). Relative amounts of toxin were normalized to total proteins.
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bioRxiv - Cancer Biology 2020Quote: ... Supernatants were harvested and the protein concentration was measured using the Pierce BCA protein assay kit (ThermoFisher Scientific, 23225). Samples were mixed with NuPAGE LDS sample buffer (ThermoFisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... The identified proteins were filtered using the high peptide confidence setting in Protein Discoverer (version 2.2, Thermo Fisher Scientific), which correspond to a filter for 1% FDR on peptide level ...
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bioRxiv - Bioengineering 2019Quote: ... Protein concentration for each sample was estimated in a single technical replicate using the Qubit Protein Assay Kit (Invitrogen) and the Qubit 3.0 Fluorometer (Invitrogen).
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bioRxiv - Cancer Biology 2019Quote: ... followed by incubation with 7.5 µL each of Protein A- and Protein G-Dynabeads (Life Technologies 10001D and 10003D) at 4°C for 1 hour ...
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bioRxiv - Neuroscience 2019Quote: ... The supernatant was saved and protein concentration was quantified using Pierce BCA protein assay kit (ThermoFisher Scientific, Waltham, MA). Protein samples were denatured for 5 min ...
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bioRxiv - Genomics 2020Quote: ... Pooled fractions were tumbled at 4°C for 1 hour with 5μg of antibody (Key Resources Table) and 25μL Dynabeads™ Protein A or Protein G (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: Protein lysates were prepared in either RIPA or CHAPS buffer and quantified using the BCA Protein Assay (Thermo Scientific). Protein was separated on 4%–20% SDS-PAGE gels ...
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bioRxiv - Immunology 2019Quote: ... Protein concentrations of whole-cell lysate fractions were then determined using the BCA Protein Assay Reagent kit (Thermo Scientific) according to manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... The amount of proteins of each sample was measured with Pierce BCA protein Assay Kit (Thermo Fisher, Waltham, MA) and normalized to the smaller concentration obtened ...
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bioRxiv - Immunology 2021Quote: ... Samples were quickly spun down and protein concentration in the supernatants was quantified by BCA protein assay (Thermo scientific). Co-cultures were performed by adding T cell clones that had rested overnight in fresh media without IL-2 to wells containing DCs ...
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bioRxiv - Genetics 2019Quote: ... Whole protein concentration was measured using Pierce BCA Protein Assay Kit according to the manufacturer’s protocol (Thermo Fisher Scientific). SDS-Page separation was completed by running 2 μg of total protein on a NuPAGE™ 3-8% Tris-Acetate gel (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2021Quote: ... The protein concentration of each sample was analyzed using a Pierce™ BCA Protein Assay Kit (Thermo Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The suspension was incubated with preequilibrated protein A/G magnetic beads (Thermo Fisher Scientific; 20 μl slurry/mg protein) for 1 hour at 4°C with gentle shaking ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were separated on NuPAGE™ Novex® 4-12% Bis-Tris protein gels in MES buffer (Life Technologies) and were transferred onto nitrocellulose membranes using the iBlot® system (Life Technologies) ...
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bioRxiv - Biochemistry 2021Quote: The apparent melting temperature (Tm) of the proteins was determined according to a protein thermal shift assay (ThermoFisher Scientific) based on using SYPRO orange ...
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bioRxiv - Immunology 2020Quote: Purified RBD protein (100 ug) was labeled with Alexa Fluor 488 using microscale protein labeling kit (Life Technologies, #A30006) as per manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Antibody proteins were purified from the culture supernatant after 12-14 days using Protein A bead columns (Thermo Fisher).
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bioRxiv - Cell Biology 2021Quote: ... Total protein concentrations were determined using the Pierce™ Coomassie (Bradford) Protein Assay Kit according to manufacturer’s instructions (ThermoFisher). Equal concentrations of cell lysates were prepared in Laemmli buffer (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts (30 μg) of protein measured by the BCA protein assay kit (23225, ThermoFisher Scientific, Waltham, MA, USA) were loaded into a precast NuPAGE 4-12% gradient Bis-Tris gel ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were clarified by centrifugation and protein levels were quantified using a BCA protein assay kit (Thermo Fisher Scientific). Proteins (50 μg ...
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bioRxiv - Neuroscience 2021Quote: ... An equal amount (500µg) of each protein lysate was precleared for 1 hour with magnetic Protein G Dynabeads (Invitrogen) and immunopurified with 40µl washed anti-V5 agarose beads (Sigma ...
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bioRxiv - Plant Biology 2020Quote: The concentration of the mTurquoise2 protein was determined using a Pierce™ 660nm Protein Assay Kit (#22662, Thermo Scientific) and used as reference to build a mTurquoise2 standard curve (linear regression ...
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bioRxiv - Plant Biology 2020Quote: ... Total soluble protein concentration was estimated using a Pierce™ 660nm Protein Assay Kit as above (#22662, Thermo Scientific).
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bioRxiv - Biochemistry 2021Quote: ... Protein concentrations were determined by the Pierce™ BCA Protein Assay Kit according to manufacturer’s instructions (Thermo Scientific, 23227). Samples were analyzed on a Quadrupole-Time of Flight (Q-TOF ...
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bioRxiv - Biochemistry 2021Quote: ... which was calibrated using an unstained native protein ladder (NativeMark™ Unstained Protein Standard A, Thermo Fisher Scientific Inc.). Measurements were performed on the reconstituted complex I at a concentration of 0.015 mg ml-1 using AcquireMP 2.2.0 software and were analyzed using the DiscoverMP 2.2.0 package.
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bioRxiv - Cell Biology 2021Quote: ... Lysates were sonicated and the total protein amount was determined with a BCA protein assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... A 1:5 dilution of this protein solution was quantified using a Pierce BCA protein assay kit (Thermo scientific). For analysis of the relative composition of amino acids ...
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bioRxiv - Microbiology 2021Quote: ... per reaction and 50 µL of magnetic protein G-coated Dynabeads according to the supplier’s instructions (Dynabeads Protein G for Immunoprecipitation, 10003D, ThermoFisher). Proteins were eluted in the denaturing mode and run in a 4-20% Mini-Protean TGX stain-free precast protein gel (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... The protein levels in the supernatants were determined using the BCA Protein Assay Kit (Pierce/Thermo Scientific, Rockford, IL). To detect BDNF ...
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bioRxiv - Neuroscience 2020Quote: ... The total protein concentration was measured with bicinchoninic acid protein assay (Pierce™ Micro BCA Kit, ThermoFisher Scientific, USA). Human brain-derived Aβo was characterized by Western blot analysis and atomic force microscopy (details in Additional file 1 ...
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bioRxiv - Microbiology 2021Quote: ... according to manufacturer’s specifications and protein concentration was determined using a bicinchoninic acid (BCA) protein assay (Thermo Fisher Scientific). 10 µg of nuclear extract was assayed and DNA binding activities of transcription factors (ATF-2 ...
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bioRxiv - Microbiology 2021Quote: ... Protein digestion was performed by incubating in trypsin (1 µg trypsin/50 µg protein; Thermo Fisher Scientific, Waltham, MA) for 3 h ...
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bioRxiv - Physiology 2021Quote: ... The supernatant was saved and protein was quantified using a Pierce BCA protein assay kit (ThermoFisher, catalog number 23225). 100 ug of protein was used per sample ...