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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for Recombinant Mouse Efna5 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10 % heat inactivated Fetal Bovine Serum (HI FBS) (10500064; Gibco) and 1x antibiotic-antimycotic drug (100 units/mL of penicillin ...
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bioRxiv - Biophysics 2023Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Gibco, USA) and 100 U mL-1 penicillin–streptomycin (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... His-tag purification using Ni-NTA resin (Thermo Fisher, Massachusetts, United States) was performed and rubisco was eluted by SUMO tag cleavage with bdSUMO protease (as produced in Davidi et al ...
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bioRxiv - Molecular Biology 2021Quote: ... We immunoprecipitated GFP-tagged Znf684 with 1 μg anti-GFP antibody (G10362, Life Technologies) overnight followed by a 2-hour incubation with Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... PCR8-KLK4-KLKP1 (DDK tagged) was recombined with pLenti6/V5-Dest™ (Life Technologies) or pAD/CMV/V5-Dest™ (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... and/or AlexaFluor-594-tagged goat anti-guinea pig IgG (1:500; Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and c-Myc-tagged N protein together with GFP using Lipofectamine 2000 (Life Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Each tagged protein was visualized by 1-Step Ultra TMB-Blotting Solution (ThermoFisher Scientific).
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bioRxiv - Bioengineering 2020Quote: ... sections were incubated with the appropriate Alexa 546-tagged secondary antibody (Invitrogen, Carlsbad, CA) for 2 hrs at room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged RNF219 and CNOT1 cDNAs were cloned into pCDNA5/FRT-TO vector (Invitrogen). The expression vectors were then transfected into HEK-293 Flp-In-TRex cells and followed by selection with hygromycin to generate stable cell lines ...
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bioRxiv - Molecular Biology 2019Quote: ... GST-tagged GSK3β constructs were cloned into the pDEST™27 vector (ThermoFisher Scientific) as described previously66 ...
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bioRxiv - Biochemistry 2021Quote: ... GST-tagged RASSF5 (GST-RASSF5) sequence was transferred into the pDEST15 expression vector (Thermofisher), and then expressed and purified from BL21-A1cells ...
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bioRxiv - Immunology 2022Quote: ... and RAW 264.7 EROS FLAG-tagged cells were maintained in DMEM medium (21969035, ThermoFisher) containing 10 % FBS (F7524 ...
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bioRxiv - Biophysics 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Immunology 2022Quote: ... was used to generate Flag-tagged Prom1 clones and deletion mutants in pcDNA3.1+ (Invitrogen). The primers used for cloning are listed in Supplementary Table 1.
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bioRxiv - Neuroscience 2023Quote: ... N2A cells were transfected with different HA tagged WDR47 variants using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... 6xHis-tagged CapZ was purified as previously described30,49 using HisPur Cobalt resin (Thermo Scientific), and separated from high-molecular weight contaminants by size-exclusion chromatography using a Sephacryl S-200 HR column (Cytiva/GE Healthcare) ...
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bioRxiv - Biophysics 2024Quote: ... followed by incubation with secondary antibodies tagged with Alexa fluorophores (Invitrogen, A11034; Invitrogen, A21422). Samples were mounted with media containing DAPI (Southern Biotech ...
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bioRxiv - Biophysics 2024Quote: ... followed by incubation with secondary antibodies tagged with Alexa fluorophores (Invitrogen, A11034; Invitrogen, A21422). Samples were mounted with media containing DAPI (Southern Biotech ...
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bioRxiv - Bioengineering 2023Quote: ... A fluorescently tagged secondary antibody (Goat anti-rabbit, Alexa Fluor 647, Thermo Fisher Scientific) diluted in the same antibody solution was applied for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 488-tagged donkey-α-goat secondary antibody (1:1000; Molecular Probes) for one hour ...
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bioRxiv - Developmental Biology 2022Quote: ... and tagged Stat3 were synthesized with linearized plasmids using T7 mMessage mMachine kit (Invitrogen). For overexpression essays ...
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bioRxiv - Cell Biology 2023Quote: ... and His6-tagged VASP (Benz et al., 2009) using Lipofectamine 2000 (Invitrogen, Karlsruhe, Germany). Cells were lysed in buffer A (40 mM HEPES-NaOH ...
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bioRxiv - Molecular Biology 2023Quote: ... WRN Halo-tagged U2OS cells were seeded in GlutaMAX- supplemented DMEM (Gibco, 10566-016) with 10% FBS and 1% Penicillin/Streptomycin at 6x103 cells per well ...
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bioRxiv - Developmental Biology 2023Quote: ... The primary labelling was detected using appropriate Alexa-Flour tagged secondary antibody (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2024Quote: NusA-Strep-tagged DDX6 was produced in E.coli BL21(DE3) Star cells (Thermo Fisher) in LB medium at 20°C ...
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bioRxiv - Cell Biology 2020Quote: ... 20 ng/ml recombinant human epidermal growth factor (Invitrogen, USA), 10 ng/ml human basic FGF (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... human recombinant Epidermal Growth Factor (5 ng/ml, ThermoFisher Scientific) and 0.15 mM CaCl₂ (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2020Quote: ... coated with 5μg/ml human recombinant vitronectin (Life Technologies, #A14700) in mTesR1 (StemCell Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... 0.36 pM Recombinant Human Bone Morphogenetic Protein 4 (BMP4, Gibco), and 0.08 pM Activin A (Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... and human recombinant epidermal growth factor (EGF; Gibco 10450-013). All cells were cultured at 37 °C and 5% CO2.
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bioRxiv - Immunology 2021Quote: All recombinant proteins were produced using Expi293F cells (Life Technologies). Spike proteins for enzyme-linked immunosorbent assay (ELISA ...
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bioRxiv - Microbiology 2019Quote: ... and recombinant epidermal growth factor (10 ng/ml; Gibco-Invitrogen) at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: ... and recombinant epidermal growth factor (10 ng/ml; Gibco-Invitrogen) at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: Glial-Derived Neurotrophic Factor (GDNF) Recombinant Human Protein (ThermoFisher, #PHC7044)
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bioRxiv - Neuroscience 2020Quote: Brain-Derived Neurotrophic Factor (BDNF) Recombinant Human Protein (ThermoFisher, #PHC7074)
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: ... with RNaseOUT™ Recombinant Ribonuclease Inhibitor (Invitrogen cat# 10777-019).
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bioRxiv - Cell Biology 2021Quote: ... RNaseOUT recombinant ribonuclease inhibitor (200 U/mL; Thermo Fisher Scientific) and IGEPAL® CA-630 (0.5 % ...
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bioRxiv - Immunology 2021Quote: ... and recombinant epidermal growth factor (EGF, 2 ng/ml, Gibco).
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bioRxiv - Bioengineering 2020Quote: ... 12.5μg/ml Insulin human recombinant zinc solution (Thermo Fisher 1258014)] ...
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bioRxiv - Biochemistry 2020Quote: ... 40 U RNase inhibitor (RNaseOUT ™ Recombinant Ribonuclease Inhibitor, Invitrogen), and various amount of DNA sample (see figures) ...
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bioRxiv - Biophysics 2021Quote: Recombinant constructs were expressed in E.coli BL21 DE3 cells (Invitrogen) in Luria Bertani media ...
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bioRxiv - Neuroscience 2019Quote: ... 200 units/ml RNaseOUT™ Recombinant Ribonuclease Inhibitor (#10777019; Thermofisher), and 1X cOmplete™ ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant protein was expressed using Expi293F™ Cells (ThermoFisher Scientific) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... recombinant proteins or commercially available RNase T1 (Thermo Fisher Scientific) were mixed with 0.5 pmol substrate RNAs in 10 µl RNase reaction buffer with EDTA (20 mM Tris-HCl pH7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 10 ng/mL recombinant human TRAIL (Life Technologies, PH1634) in serum-free medium for 4 hours ...
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bioRxiv - Cell Biology 2021Quote: ... and 40 U/μL of Recombinant Ribonuclease Inhibitor RNaseOUT (Invitrogen) at 25°C for 2 minutes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were transfected with recombinant bacmids using Cellfectin reagent (Life Technologies). After a three-day incubation period ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant monoclonal cystatin A antibodies (Thermo Fisher Scientific, MA5-29200) were immobilized on the silica surface of the microtoroid through EDC/sulfo-NHS covalent coupling ...