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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for Recombinant Mouse ALCAM Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... Immunostaining of BBB constructs for tight junction protein ZO-1 was conducted using ZO-1 mouse monoclonal antibody conjugated with Alexa 488 (ThermoFisher). Hydrogels were removed from the transwell insert using a biopsy punch ...
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bioRxiv - Neuroscience 2021Quote: ... The tandem mass spectra were searched using SEQUEST 80 search algorithms against a Uniprot database (human 2019_07 release for 293T CoIP and mouse 2019_08 release supplemented with human TDP-43 protein sequence for mouse CoIP) through the Proteome Discoverer platform (version 2.5, Thermo Scientific). The search parameters included a maximum of two missed cleavages ...
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bioRxiv - Cell Biology 2021Quote: ... Each protein of interest was then detected with HRP-conjugated goat anti-rabbit or anti-mouse IgG antibody (1:2000; Invitrogen), and visualized using SuperSignal West Pico PLUS Chemiluminescent Substrate or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... Secondary detection of bait proteins was performed in 2.5% bovine serum albumin (BSA) in PBS using goat anti-mouse Alexa FluorTM 488 (Molecular Probes, ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The Mex67-3Flag tagged protein was isolated using 200 µL of pre-washed magnetic Pan Mouse Dynabeads (Thermo Fisher Scientific) coupled to 34 µg of anti-Flag M2 mouse monoclonal antibody (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... The autophosphorylation of hIRE1 KR was confirmed by western blot using a pSer/24 phospho-specific antibody (Genentech) and phosphorylated Pumilio proteins were detected using anti-mouse-V5 antibody (Invitrogen). In the case of kinase radioactive assays ...
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bioRxiv - Molecular Biology 2022Quote: ... The combined data set of 9 injections were mapped against the mouse protein database using Proteome Discoverer v2.4 and the Sequest HT search algorithm (Thermo Scientific) with label-free quantitation (LFQ ...
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bioRxiv - Neuroscience 2021Quote: NAc samples were pooled from four D1-Cre-RT or D2-Cre-RT male and female mice and RNA isolation from immunoprecipitated polyribosomes using primary mouse anti-HA antibody (Covance Research Products, Cat#MMS101R) and secondary antibody coated magnetic Dynabeads (Dynabeads protein G, Invitrogen) to pulldown the MSN specific RNA ...
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bioRxiv - Molecular Biology 2020Quote: ... TAP purification was carried out as previously described 48 with 2 mg of protein extract and 50 μl of Dynabeads Pan Mouse IgG (Invitrogen) per sample ...
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bioRxiv - Microbiology 2020Quote: ... Cleavage of junctional proteins was detected by Western blot analysis using specific antibodies against E-cadherin (Mouse, mAb, Thermo Scientific), p120-catenin (Rabbit ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The presence of AhR and ARNT proteins was verified by Western blot using anti-His-tag (mouse monoclonal, MA1-21315, dilution 1:1000, Invitrogen) anti-FLAG-tag (rabbit monoclonal ...
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bioRxiv - Immunology 2020Quote: ... slides were stained with the following secondary antibodies: polyclonal goat anti-Mouse Alexa Fluor488 to detect CD68 protein (Green color) (A-11029, Invitrogen) or polyclonal goat anti-Rabbit Alexa Fluor546 to detect SP140 protein (Orange color ...
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bioRxiv - Neuroscience 2022Quote: ... 50 and 35µg of proteins (for cell lysates, mouse brains and human cortices, respectively) were added with 1x Sample buffer (Thermo Fisher) and 1x Sample Reducing Agent (Thermo Fisher) ...
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bioRxiv - Evolutionary Biology 2022Quote: We synthesized the Bowhead whale CDKN2CRTG protein with mouse codon usage (GeneScript) and directly cloned the synthesized gene into the pcDNA3.1-C-eGFP vector (Life Technologies), which added a C-terminal eGFP tag ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... proteins were extracted from homogenized mouse placental tissues or cell samples using RIPA lysis buffer (89900, Thermo Fisher Scientific, USA) containing protease and phosphatase inhibitors (A32961 ...
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bioRxiv - Cancer Biology 2022Quote: ... The horseradish peroxidase (HRP)-conjugated Protein A or HRP-conjugated rabbit anti-mouse secondary antibody for immunoblotting were from Invitrogen. The alpha smooth muscle actin (# ab7817) ...
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bioRxiv - Molecular Biology 2023Quote: The raw data files acquired from each sample were searched against mouse protein sequences database using the Proteome Discoverer 1.4 software (Thermo Scientific) based on the SEQUEST algorithm ...
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bioRxiv - Cancer Biology 2023Quote: 20 μg protein per sample was used for the IFN-gamma mouse ProcartaplexTM Simplex High Sensitivity kit (Thermo Fisher Scientific) and the protocol was followed as per the product manual ...
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bioRxiv - Genetics 2022Quote: ... About 50ug total protein from cell extracts were blotted and hybridised with the primary antibody of Mouse anti-V5 (Invitrogen) for CLK and BMAL1 expression then the secondary antibody of horseradish peroxidase-conjugated either anti-mouse or anti-rabbit IgG antibody (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: The raw spectra were searched against mouse protein database (UniProt) by SEQUEST using Proteome Discoverer (v2.5, Fisher Scientific, Waltham, MA). The mass tolerance was set as 20 ppm for precursors and 0.5 Da for fragments ...
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bioRxiv - Neuroscience 2023Quote: ... coated 10-cm dishes at a density of 100,000 cells/cm2 or on coverslips coated by 20 µg/ml poly-D-lysine and 10 µg/ml laminin mouse protein (Gibco) at a density of 40,000 cells/cm2 in plating medium (high glucose DMEM medium containing L-glutamine and 10% fetal bovine serum) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the membrane protein lysates were incubated with 10 μg mouse monoclonal anti-ubiquitin (eBioP4D1) antibody (Invitrogen, 14-6078-82) at 4 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... HKU1 NTD proteins were either precomplexed with StrepMAB-Classic HRP (IBA) and secondary goat anti-mouse IgG Alexa Fluor 555 (Invitrogen) in a 4:2:1 molar ratio or conjugated with pA-LS NP in a 1:1 molar ratio ...
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bioRxiv - Physiology 2024Quote: ... and TaqMan primers (mouse Panx1 mm0045091, mouse Panx2 mm01308054, mouse Pan×3 mm00552586, mouse Gja1 mm00439105, mouse Gja5 mm00433619– Invitrogen) (Rat Panx1 Rn01447976_m1 – Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Cell proteins and EVs proteins were quantified by Qubit Protein Assay (Invitrogen) using a Qubit 3.0 Fluorometer (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... and recombinant SHH was purified with Detoxi-Gel™ Endotoxin Removing Gel (ThermoFisher) and dialyzed ...
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bioRxiv - Plant Biology 2020Quote: All recombinant plasmids were generated by the GATEWAY cloning system (Invitrogen, http://www.invitrogen.com/). The primers used for gene amplification are listed in S3 Table ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant lentivirus was produced by transfecting HEK293T cells with Lipofectamine 2000 (Invitrogen, 11668019) or TransIT-2020 transfection reagent (Mirus ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant adenovirus plasmids were homologously recombined with pAd/CMV/V5-DEST vectors (Invitrogen), as per manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... and 50 ng/ml human recombinant M-CSF (ThermoFisher Scientific, Rockford, IL, USA). After six days of PBMC culture ...
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bioRxiv - Developmental Biology 2020Quote: ... Recombinant lentiviruses were produced by transient transfection in HEK293FT cells (Invitrogen, CA, USA), as described earlier 18 ...
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bioRxiv - Genomics 2019Quote: ... 4) We used RNase Inhibitor in place of RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen) in the first-strand RT reaction ...
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bioRxiv - Microbiology 2021Quote: ... 10 ng/ml of recombinant human fibroblast growth factor 2 (FGF2, Life Technologies), 20 ng/ml of recombinant human epidermal growth factor (EGF ...
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bioRxiv - Cell Biology 2020Quote: ... except that a final concentration of 10 nM recombinant PKR (Life Technologies # PV4821) and 1 mM ATP was added during incubation with FLAG M2 magnetic beads ...
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bioRxiv - Bioengineering 2021Quote: ... The cell passage was performed using recombinant cell-dissociation enzyme (TrypLE, Gibco™) to detach cells followed by neutralizing with culture medium ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant aFGF/FGF-1 and bFGF/FGF-2 were purchased from Thermo Fisher Scientific (13241-103 and PHG0024) ...
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bioRxiv - Biophysics 2020Quote: ... and stimulated with 50 U/mL recombinant interleukin-2 (IL-2, Thermo Fisher) and 5 μg/mL phytohaemagglutinin (PHA ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 10 ng/ml.
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bioRxiv - Cell Biology 2021Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 10 ng/ml.
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bioRxiv - Microbiology 2020Quote: ... coli-derived recombinant BAG1) and secondary goat anti-rabbit 594 (1:1000, Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... Recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Biophysics 2020Quote: ... Recombinant constructs were co-expressed with BirA in E.coli BL21 DE3 cells (Invitrogen) in Luria Bertani media supplemented with 50 mM biotin ...
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bioRxiv - Microbiology 2022Quote: ... the recombinant 3CLpros were expressed in Escherichia coli BL21 cells (Invitrogen, Carlsbad, CA) grown in Luria Bertani broth induced with 1mM isopropyl ß-D-thiogalactopyranoside ...
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bioRxiv - Developmental Biology 2022Quote: ... The recombinant plasmids were introduced into HEK293-EBNA cells (Invitrogen, Carlsbad, CA, USA) using FuGENE 6 transfection reagents (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... Recombinant zebrafish Nid2a and Hmcn1 fragments were generated in HEK293-EBNA cells (Invitrogen) and purified as described above for the mouse proteins and used to immunize rabbits or guinea pigs (Pineda Antikörper Service ...
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bioRxiv - Neuroscience 2021Quote: ... recombinant TTR was detoxified using a high-capacity endotoxin removal resin (Thermo Scientific) and quantified using the Lowry based DC Protein Assay (Bio-Rad Laboratories).
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bioRxiv - Microbiology 2020Quote: ... the recombinant plasmids were linearized with PacI before transfection with Lipofectamine 2000 (ThermoFisher) into the adenovirus packaging cell lines HEK293 cells ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 20 U/ml RNase-free Recombinant DNase I (Invitrogen, Cat # AM2235) and incubated for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant BACs were purified using PureLink™ HiPure Plasmid Midiprep Kit (ThermoFisher Scientific) and injected into 1-cell stage zebrafish embryos together with I-SceI (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were expanded on human recombinant vitronectin in StemFlexTM medium (Thermo Fisher Scientific). iBEC differentiation was performed as previously described by us [18] ...