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Citations for Thermo Fisher :
901 - 950 of 7252 citations for Recombinant Human VAPB His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (HI-FCS, Gibco) and 1% of Penstrep (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were taken for immunoblotting using anti His-tag antibody (ThermoFisher). Crosslinking and control reactions intended for scramblase activity assays were stopped with 1 M Tris pH 7 to a final concentration of 10 mM ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% (v/v) heat-inactivated FBS (HI-FBS; Gibco). Immortalised adult human C20 microglial cells were kindly provided by Dr David Alvarez Carbonell ...
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bioRxiv - Biochemistry 2023Quote: ... All media was supplemented with 10% HI FBS (Gibco cat # 10438026).
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bioRxiv - Biochemistry 2023Quote: ... Hi Five cells grown in Sf-900 II SFM media (Gibco) at a density of 2.0 × 106 cells/mL were infected with baculovirus at 1.5% v/v ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with Novex Hi-Density TBE Sample buffer (ThermoFisher Scientific, LC6678) and analysed on 10% Novex TBE gels (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 10% fetal bovine serum (Gibco FBS HI, Cat# A5256701) and 1% penicillin-streptomycin (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... followed by Western blot analysis using anti-His antibody (Invitrogen, USA).
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bioRxiv - Microbiology 2023Quote: ... 2 µL of Novex Hi-Density TBE 5X Sample Buffer (Invitrogen) was mixed with 8 µL of the completed EMSA reaction and loaded on an 8% acrylamide gel ...
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bioRxiv - Biophysics 2023Quote: ... To cut the His-tag HRV 3C protease (Thermo-Fisher Scientific) was added to a molar ratio of 1:800 (enzyme ...
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bioRxiv - Cancer Biology 2021Quote: ... The HA-tagged cDNAs were subcloned into the pLenti6-D-TOPO vector (Invitrogen). The cDNA for human GSK3βwas subcloned into the pCMV- myc vector ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-tagged proteins were immuno-precipitated with the A11122 antibody (Thermo Fisher Scientific); Myc-tagged proteins were immuno-precipitated with the 9E10 antibody (Merck) ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by the Alexa-tagged secondary antibodies (Alexa 488; A21206, Invitrogen) diluted in 7% serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... GFP-tagged viral proteins were immunoprecipitated with anti-GFP antibody (G10362, Life Technologies) overnight followed by a 2-hour incubation with Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Neuroscience 2019Quote: ... we performed bilateral injections of fluorescently-tagged polystyrene microspheres (10 micron, Invitrogen F8836) into the anterior chambers of 6-8 weeks old mice (Calkins et al. ...
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bioRxiv - Cell Biology 2021Quote: ... LDs were labeled by BODIPYTM 558/568 (FA568, fluorescent tagged oleic acid, Thermofisher) or LD540 (Spandl et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Cholera toxin subunit B (CTXβ) tagged with Alexa 555 or 647 (Molecular Probes) for histology experiments.
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bioRxiv - Microbiology 2021Quote: ... Tagged proteins were visualized using the SuperSignal™ West Femto detection kit (ThermoFisher).
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent detection was done with an Alexa- tagged secondary antibody from Molecular Probes, donkey anti-sheep (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Assay mixes with 1 nM GST-tagged GR (aa 521-777, Invitrogen #PV4689), 25 nM Alexa-488 conjugated GST antibody (Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... The V5-AID tagged Brn1 was detected with anti-V5 antibody (Invitrogen, R96025) at a 1:4000 dilution ...
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bioRxiv - Bioengineering 2019Quote: ... one-hour incubation of Alexa 568 tagged secondary antibody (Fisher Scientific, Hampton, NH) performed and stained with DAPI (Vecor Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... T cells were tagged using the Cell Trace Far Red dye (ThermoFisher Scientific) prior to injection into the slices ...
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bioRxiv - Microbiology 2021Quote: ... followed by fluorescence tagged goat anti-rabbit IgG Alexafluor 488 (1:200, Invitrogen) and fluorescence tagged goat anti-mouse IgG Alexafluor 594 (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa 555 or Alexa 594 tagged secondary antibodies (Invitrogen, Germany, dilution 1:2000) were used ...
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bioRxiv - Cell Biology 2022Quote: His8-tagged NR2B proteins were labelled with Alexa 647 NHS ester (Thermo Fisher) and PrLD-SAMWT/PrLD-SAMME/PrLD proteins labelled with Alexa 555 maleimide (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... and Alexa Fluor 647-tagged donkey anti-rabbit IgG (1:500, Thermo Fisher, catalog # A-31573 ...
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bioRxiv - Microbiology 2023Quote: ... then incubated with Alexa-488 tagged secondary antibody (goat anti-mouse IgG, Invitrogen) at a dilution of 1:500 ...
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bioRxiv - Biophysics 2023Quote: ... After incubation with appropriate HRP-tagged secondary antibodies and chemiluminescent substrate (Thermo Fisher), the blots were imaged and processed with a FlourChem E (Protein Simple) ...
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bioRxiv - Cell Biology 2023Quote: ... or S1S2 constructs tagged with FLAG epitope were transfected using Lipofectamine 3000 (Invitrogen) as described previously [11].
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bioRxiv - Developmental Biology 2023Quote: ... cells/sections were treated with fluorescence (Cy3/FITC)-tagged secondary antibodies (Life Technologies) diluted in 5% donkey serum and 0.2%/0.4% Triton X-100 solution for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... The GST-tagged proteins were resuspended in phosphate-buffered saline (PBS) (Thermo Fisher) containing 1 mM dithiothreitol (DTT ...
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bioRxiv - Plant Biology 2023Quote: ... Myc-tagged proteins were detected using SuperSignal West Femto: SuperSignal substrates (ThermoFisher Scientific) in a 1:1 ratio ...
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bioRxiv - Neuroscience 2023Quote: ... with Flag-tagged siRNA resistant TDP-43 or pRc/CMV control vector (Invitrogen), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... After incubation with appropriate HRP-tagged secondary antibodies and chemiluminescent substrate (Thermo Fisher), or purified LgBiT-substrate cocktail (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... HTP-tagged proteins were immunoprecipitated with IgG-coupled Dynabeads (tosylactivated M280, Thermo Scientific), RNAPII with antibody against Rpb1 (8WG16 ...
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bioRxiv - Molecular Biology 2023Quote: ... Alexa 488 or Alexa 594 tagged secondary antibodies (Invitrogen, Germany, dilution 1:1000) were used ...
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bioRxiv - Biochemistry 2024Quote: ... AncΔ5 were tagged with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher), followed by the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2021Quote: ... recombinant murine EGF (50 ng/ml; Thermo Fisher Scientific), recombinant murine Noggin (100 ng/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... including 1.25 U Taq recombinant DNA polymerase (Invitrogen, USA), 1x PCR Buffer (-Mg) ...
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bioRxiv - Molecular Biology 2021Quote: ... recombinant murine EGF (50 ng/mL; Thermo Fisher Scientific), recombinant murine Noggin (50 ng/mL ...
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bioRxiv - Genomics 2020Quote: ... treated with RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific). Library concentrations were determined using Qubit Fluorometer (v.4.0 ...
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bioRxiv - Genomics 2020Quote: ... treated with RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific). Library concentration was determined using Qubit Fluorometer (v.4.0 ...
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bioRxiv - Neuroscience 2019Quote: ... Briefly recombinant Protein G Agarose beads (ThermoFisher cat# 15920010) were washed three times with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... and RNaseOUT™ Recombinant Ribonuclease Inhibitor (ThermoFisher Scientific # 10777019) according to manufactures instructions ...
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bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgG Fab recombinant secondary antibody (Invitrogen), anti-NKp46 (9E2 ...
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bioRxiv - Genetics 2021Quote: ... Extracted RNA was treated with Recombinant DNaseI (Thermo Scientific) for 30 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... 25 µL packed Recombinant Protein A-Sepharose 4B (Invitrogen) was used to capture the immune complexes for 1 hour at RT ...
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bioRxiv - Genetics 2022Quote: ... and 200 μg/mL recombinant proteinase K (Thermo Scientific). The mixture was incubated at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 40U RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen, Antisel SA, Hellas) and 4 μM random hexamer primers (Takara Bio ...