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Citations for Thermo Fisher :
901 - 950 of 8720 citations for Recombinant Human LDLR His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibodies (1:2000 ...
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bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher), and 400 TRAP220 peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Molecular Biology 2019Quote: ... All proteins were expressed in Hi-Five cells (Invitrogen) from Trichoplusia ni using Baculovirus and the cultures were harvested 60 hours after infection ...
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bioRxiv - Biochemistry 2021Quote: ... Hi-5 cells (BTI-TN-5B1-4) (Gibco #B85502) were cultured in Express Five™ SFM (Serum-Free Media ...
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bioRxiv - Immunology 2021Quote: 100 µL of Invitrogen His-Tag Dynabeads (ThermoFisher 10104D) were used for prefusion S2 depletion ...
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bioRxiv - Developmental Biology 2020Quote: ... and cloned into the pAc5.1-V5/His vector (Invitrogen). Immunoprecipitations between Bdbt-EGFP and Myc-DAnkrd49 used GFP rabbit serum (ab290 ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were cloned into pcDNA-V5/His (Invitrogen) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... The pcDNA4-TO-myc/his B backbone vector (Invitrogen) was chosen for overexpression ...
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bioRxiv - Molecular Biology 2022Quote: ... 15 mL 6x His-Tag Monoclonal antibody (MA121315; Invitrogen) diluted 1:1500 in blocking solution supplemented with 0.1% NaN3 was added to the membrane and incubated at 4°C on an orbital shaker at 55 rpm for 14-16 h ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-His antibody (Invitrogen cat. # MA1-21315-1MG) was used at the same concentration to coat the plates ...
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bioRxiv - Microbiology 2022Quote: ... anti-His×6 (mouse monoclonal, Invitrogen, 1:10,000-20,000), anti-OmpA (rabbit polyclonal ...
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bioRxiv - Biochemistry 2024Quote: Supernatant was supplemented with His-Pur cobalt resin (ThermoFisher), imidazole to 10 mM ...
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bioRxiv - Immunology 2024Quote: ... 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) were coupled with 200 µg of histidine-tagged SARS-CoV-2 S protein ...
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bioRxiv - Cell Biology 2022Quote: ... were cloned in pcDNA3.1(-) Myc/His vector (ThermoFisher Scientific). Ligated plasmids were transformed in chemically competent E ...
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bioRxiv - Cell Biology 2023Quote: ... and into pcDNA™6/myc-His A (Invitrogen), from an amplicon produced by RT-PCR from S2Xpress RNA ...
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bioRxiv - Biochemistry 2023Quote: ... His-Tag Dynabeads (Invitrogen 10103D, 10 µL per sample) was washed three times with B buffer before protein immobilization ...
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bioRxiv - Biophysics 2023Quote: His-Tag Dynabeads (Invitrogen 10103D, 10 μL per sample) were manually washed three times with B buffer and resuspended in B buffer (100 μL per sample) ...
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bioRxiv - Plant Biology 2023Quote: ... Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific) were washed twice in Strep-to-His Buffer before adding the eluted proteins and incubating while rotating for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... araC and the araBAD promoter from pBAD/His (Invitrogen), the tetracycline resistance gene from pACE2 (Geneva Biotech ...
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bioRxiv - Bioengineering 2024Quote: ... 10% heat-inactivated fetal bovine serum (HI-FBS; Gibco), 100 U ml−1 penicillin/100 μg ml−1 streptomycin (Gibco) ...
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bioRxiv - Molecular Biology 2024Quote: ... and cloned into pcDNA3.1-myc/His A vector (Invitrogen) with restriction enzymes ...
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bioRxiv - Biophysics 2024Quote: ... 1 nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher), and 400 nM FITC-labeled NCoR1 ID2 or MED1 ID2 peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Biochemistry 2024Quote: ... coli was inserted into pBAD/Myc His A (Invitrogen) resulting in an open reading frame encoding the sequence given by Uniprot IDP07287 except that the 82 carboxy-terminal residues ...
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bioRxiv - Biochemistry 2024Quote: ... 1 nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher), and 400 TRAP220 or NCoR peptide in TR-FRET buffer (20 mM KPO4 pH 7.4 ...
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bioRxiv - Microbiology 2019Quote: ... Raw cells were transfected with 7ug reporter RNAs either with or without 30 pmoles of biotinylated miR-142-3p mimic or scrambled 3’ biotinylated mimic (Dharmacon) using the Neon Transfection System (Invitrogen; 1750V, 25ms, 1 pulse). At 1.5-2h post-transfection ...
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bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 antigens were biotinylated using EDC (Thermo Fisher) and Sulfo-NHS-LCLC biotin (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: ... All targets were chemically biotinylated using NHS-Biotin (ThermoFisher #20217) according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... biotinylated junctions were isolated with streptavidin beads (65601, Life Technologies); and DNA libraries were prepared ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated antigens were coupled to FluoSphere NeutrAvidin beads (Thermo Fisher) and incubated with 10 μl 1:100 diluted plasma for 2h at 37°C to form immune-complexes ...
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bioRxiv - Immunology 2022Quote: ... biotinylated antigens were coupled to FluoSphere NeutrAvidin beads (Thermo Fisher) and incubated with 10 μl 1:100 diluted plasma for 2h at 37°C to form immune complexes ...
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bioRxiv - Immunology 2022Quote: ... biotinylated antigens were coupled to FluoSphere NeutrAvidin beads (Thermo Fisher) and to form immune-complexes incubated with 10 μl 1:10 diluted plasma samples for 2h at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... The biotinylated proteins were detected using streptavidin-HRP (Thermo Scientific) according to Hung et al. ...
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bioRxiv - Genomics 2021Quote: ... deoxynucleotides (A,G,C (sigma) and biotinylated-dUTP (ThermoFisher Scientific)) to a final concentration of 100 nM ...
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bioRxiv - Biochemistry 2020Quote: ... and biotinylated donkey anti-rabbit IgG (Cat.# A16027; Thermo Fisher).
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bioRxiv - Neuroscience 2020Quote: ... An anterograde tracer of 5% biotinylated dextran amine (BDA; Invitrogen) was used in some cases ...
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bioRxiv - Cell Biology 2021Quote: ... The biotinylated DNA was coupled to Dynabeads Kilobinders (Thermofisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... was biotinylated using biotin-NHS (Thermo Scientific, catalog number: 21343). Flow chambers for microscopy were assembled by sandwiching a piece of double-sided tape between a slide and a plasma cleaned coverslip (Fisherbrand ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated proteins were captured with streptavidin agarose beads (Thermo Fisher) at 4°C for 1 h ...
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bioRxiv - Bioengineering 2022Quote: ... plates were incubated with 250ng biotinylated protein L (Thermo Scientific) in 3% M-PBS for 1h ...
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bioRxiv - Immunology 2021Quote: ... NVX-CoV2373 Spike protein was biotinylated using EDC (Thermo Fisher) and Sulfo-NHS (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... NVX-CoV2373 Spike protein was biotinylated using EDC (Thermo Fisher) and Sulfo-NHS (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... Biotinylated proteins were precipitated using streptavidin-agarose beads (ThermoFisher Scientific) at 4°C for 2h ...
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bioRxiv - Microbiology 2021Quote: ... and biotinylated proteins were detected using streptavidin-HRP (Invitrogen S911). HRP was detected using enhanced chemiluminescence (ECL ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigens were biotinylated using EDC (Thermo Fisher) and Sulfo-NHS-LCLC biotin (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... All probes were ordered biotinylated at the 3’ end (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... Biotinylated ASOs were synthesized by RevertAid Reverse Transcriptase (Thermo Scientific) and 5’ biotin-TEG primer ...
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bioRxiv - Cell Biology 2020Quote: ... lectins were biotinylated by NHS-ester conjugation (Thermo Fisher Scientific) and dialyzed against DPBS−/− overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Biotinylated proteins were isolated using a Streptavidin (Thermo-Fisher Scientific) pull-down assay ...
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bioRxiv - Neuroscience 2021Quote: Secondary antibodies: Biotinylated anti-rabbit (1:500, Invitrogen, Carlsbad, USA). Fluorescent anti-rat (Alexa 594 ...