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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for Interstitial collagenase MMP 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the HEK293-derived cells Flp-InTM T-RexTM 293 Cell Line (ThermoFisher Scientific) were stably transfected to express HA-TDP-43 upon induction with tetracycline (1 μg/mL) ...
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Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availabilitybioRxiv - Cell Biology 2023Quote: ... HEK293 Phoenix cells were transfected with the plasmid using Lipofectamine 3000 (ThermoFisher Scientific). The transfected cells were incubated at 37°C for 5 hr ...
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bioRxiv - Developmental Biology 2024Quote: ... HEK293 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293-T cells were seeded in 10-tray Cell Factories (Thermo Fisher Scientific) 24 hours before transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 cells were transfected using Lipofactamine 2000 according to the manufacturer’s instructions (Invitrogen).
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bioRxiv - Microbiology 2021Quote: Cell suspensions or cell-free supernatants were tested for collagenase activity with the EnzChek Gelatinase/Collagenase assay kit (Invitrogen, Carlsbad, CA) using fluorescein- labelled DQTM gelatin conjugate (type I collagen ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... Pellet 1 was subjected to a second round of digestion in 1 mL of 1000 U/mL Collagenase type II and 1 mL of 11 U/mL Dispase II (Thermofisher, Cat# 17105041) along with the 8 mL of the remaining cell suspension ...
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bioRxiv - Developmental Biology 2021Quote: ... The colonies were lifted with a 1:1 Dispase/Collagenase enzyme mix (1mg/ml; Gibco; 17105-041 and 2 mg/ml; Gibco; 17104-019). and resuspended in 10 ml of Phase 1 medium (1:1 IMDM (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... the resulting suspension containing individual cells was separated from tissue fragments by sedimentation in a 50 mL falcon tube and collagenase III was neutralized adding a 1:1 amount of DMEM/F12 medium (Gibco, 11330-032) containing 15 mM HEPES and 10% FCS (Gibco ...
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bioRxiv - Biochemistry 2019Quote: ... SAMS peptide substrate (His-Met-Arg-Ser-Ala-Met-Ser-Gly-Leu-His-Leu-Val-Lys-Arg-Arg, obtained from Invitrogen, USA) was used for the assay ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder, Applied Biosystems, in 10 μL of Hi-Di formamide, Applied Biosystems) and incubated at room temperature for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder [Applied Biosystems] in 10 μL of Hi-Di formamide [Applied Biosystems]) and incubated at room temperature for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Cry1 pcDNA4A-myc-his and Cry2 pcDNA4A-myc-his plasmids were double digested with NotI and XhoI FastDigest enzymes (Thermo Scientific) and used as host plasmids ...
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bioRxiv - Biochemistry 2023Quote: Plasmids of pET28a-his TEV-glnR or pET28a-his TEV-glnR derivative was firstly transformed into expression strain BL21(DE3) (Invitrogen, Inc.). then single colonies of the positive transformants were inoculated and amplified with 5 L LB broth supplemented with 50 μg/ml kanamycin at 37 °C with shaking ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast cells were incubated with 1:1000 dilution of mouse anti-Myc antibody and 1:1000 dilution of chicken anti-His (Invitrogen, PA1-9531) antibodies on ice for 20 min ...
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bioRxiv - Immunology 2024Quote: ... at a 1:1 ratio and recombinant human IL-2 (Peprotech, ThermoFisher Scientific), and expanded in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Developmental Biology 2021Quote: ... where EGF and noggin were replaced with human forms with 10 ng ml−1 human EGF (Invitrogen, PHG0311), 100 ng ml−1 human noggin (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human IgG3 (HP6050) or mouse anti-human IgM (clone HP6083) at 1/1000 (Thermo Fisher Scientific) for 1 hour at RT ...
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bioRxiv - Cell Biology 2022Quote: ... In murine keratinocytes Dispase II solution was supplemented with 1% of collagenase I (Thermo Fisher Scientific, Massachusetts, US). Subsequently ...
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bioRxiv - Immunology 2019Quote: Tissues harvested from the extremity injury site were digested for 45 minutes in 0.3% Type 1 Collagenase and 0.4% Dispase II (Gibco) in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Evolutionary Biology 2024Quote: E18.5 male UGS were collected and collagenase-treated samples were cross-linked with 1% formaldehyde (Thermo Fisher 28908) for 10 min at RT and stored at −80° until further processing as previously described76 ...
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bioRxiv - Genetics 2020Quote: ... Germany) 1 μl of each PCR product was edited with Hi-Di Formamide (20 μl, ThermoFisher, Massachusetts, USA) and Gene Scan 500 ROX dye Size Standard (0.5 μl ...
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bioRxiv - Synthetic Biology 2021Quote: ... Anti-His immunoblots were performed using 1:2,500 diluted rabbit Anti-6xHis antibody (PA1-983B, Thermo Fisher Scientific) in 5% w/v milk in TBST for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... Uncleaved His-tagged proteins were removed by incubation of the sample with 1 ml HisPur™ (Thermo Fisher) nickel resin for 1h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were probed in PBST (PBS pH 7,4, 0,05% (v/v) Tween-20) with the mouse anti-his antibody (1:3000, Invitrogen) and the respective secondary antibody goat anti-mouse-HRP (1:3000 ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies used for the IP western blots are as follows: anti-His monoclonal (1:3000 dilution; ThermoFisher Scientific) and goat anti-mouse HRP (1:15,000 dilution ...
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bioRxiv - Biochemistry 2021Quote: ... the array was washed and probed with an anti-His primary antibody (Thermo Fisher, MA121315, 1:1000 dilution), after which anti-mouse HRP-conjugated secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... PCR was carried out with gene-specific primers (Table 1) using Phusion Hi-Fidelity PCR Mastermix (Thermo Scientific).
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bioRxiv - Microbiology 2022Quote: ... Membranes were next treated with 1: 1000 dilution of primary antibody (6-His tag mouse anti-tag, Invitrogen) for 1 hr at RT ...
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bioRxiv - Synthetic Biology 2022Quote: ... Anti-His immunoblots were performed using 1:2,500 diluted rabbit Anti-6xHis antibody (PA1-983B, Thermo Fisher Scientific) in 5% w/v milk in TBST for 1 h ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:1000 dilution of chicken anti-His antibody (Invitrogen, PA1-9531) on ice for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 10% Hi-FBS (Thermo Fisher Scientific). BSC-1 cells (ATTC ...
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bioRxiv - Neuroscience 2020Quote: ... MT5-MMP and the truncated MT5-MMP constructs (CAT-, HPX-, EXT-, TM/IC- and IC-) were synthesized and produced by GeneArt (Thermo Fisher Scientific). All these constructs were fused with a FlagM2 tag on their N-terminal part (Fig ...
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bioRxiv - Neuroscience 2020Quote: Levels of Aβ40 in supernatants of HEK or HEKswe cells transfected with different MT5-MMP variants and/or C99 constructs or treated with C3 or DAPT were evaluated by ELISA (#KHB3481, Thermo Fisher Scientific) according to the manufacturer’s recommendations.
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bioRxiv - Neuroscience 2023Quote: Gel zymography was conducted to measure the enzymatic activity of MMPs using Novex™ 10% Zymogram Plus (Gelatin) protein gels (ZY00105BOX, ThermoFisher Scientific) which contains 10% gelatin ...
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bioRxiv - Cancer Biology 2024Quote: For flow cytometry analysis of mitochondrial membrane potential (MMP) and Mitochondrial mass T cells were stained with 10 nM MitoTracker Deep Red (ThermoFisher Scientific, M22426) and 10 nM MitoTracker Green (ThermoFisher Scientific ...
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bioRxiv - Genomics 2022Quote: Frozen HEK293 or K562 cell pellets were lysed in 1 mL lysis buffer supplemented with 5 µl protease inhibitor cocktail (Thermo Fisher cat# 87786) and 10 µl Rnase inhibitor (NEB cat# M0314B ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells stably expressing CMV:DOR-APEX2 were created as previously described and maintained in 1:1000 G418/Geneticin (Thermo Fisher Scientific, Cat # 10131035) (Lobingier et al ...
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bioRxiv - Immunology 2022Quote: ... remaining tissue was digested in 30ml Collagenase solution (RPMI 1640 supplemented with 1mM MgCl2, 1mM CaCl2, 5% FBS, and 100 units/ml collagenase D (Gibco, Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Physiology 2022Quote: ... The ventricles were dissected into 3-mm2 columns and shaken at 800 rpm for 90–120 min at 30°C with 750 µL digestion buffer (12.5 µM CaCl2, 5 mg/mL collagenase type II [Thermo Fisher Scientific Inc., Waltham, MA, USA], 5 mg/mL collagenase type IV [Thermo Fisher Scientific Inc.] ...
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bioRxiv - Cancer Biology 2023Quote: ... and placed in a 15 ml falcon tube filled with 5 ml of a collagenase mixed solution (collagenase II (Gibco 17101-019) + IV (ThermoFischer 17104019) ...
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bioRxiv - Microbiology 2019Quote: ... His-FruB or His-HPr in the cell extract was purified using HisPurTM-Ni-NTA Superflow Agarose (from Thermo Scientific, Cat # 25214) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Neuroscience 2022Quote: ... and then with buffer containing 250 nM purified His-tagged protein that was previously pre-clustered with anti-His (mouse; Thermo Fisher Scientific) in a 2:1 (protein:antibody ...
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bioRxiv - Genomics 2019Quote: ... Probes were mixed with Human Cot-1 DNA (Invitrogen, 15279011) and salmon sperm (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... and 5 µg of human Cot-1 DNA (ThermoFisher Scientific) and then denatured in deionized formamide (Merck ...
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bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human FGF-2 (ThermoFisher #68-8785-63), and 1% Primocin (Invivogen #ant-pm-1 ...
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bioRxiv - Immunology 2022Quote: ... anti-Human CD279(PD-1) antibody (Invitrogen, eBioscience, PE-Cyanine7), anti-Human CD223(LAG-3 ...
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bioRxiv - Immunology 2021Quote: ... 1:100 mouse anti-human CXCR5 PE-Cy7 (MU5UBEE, ThermoFisher) was added to each well at the start of stimulation ...
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bioRxiv - Neuroscience 2020Quote: ... anti-human PHF-tauThr231 (1:1000; AT180, Thermo Scientific, USA). The secondary antibodies used against above were anti-Mouse Cy3 (1:200) ...