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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for Centrosomal Protein 63 kDa CEP63 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Antibody supernatants were passed three times over columns packed with immobilized Protein G resin (Thermo Fisher, catalog #20397) as previously described (Scherer et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Expression of Cbf11-TAP protein was verified by western blot with an anti-TAP antibody (Thermo Scientific, CAB1001). Prototrophic cbf11-TAP strain was then prepared by standard crossing and revalidated.
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bioRxiv - Genetics 2022Quote: ... Rad51 protein was detected by incubating the membranes with rabbit polyclonal antibody anti-Rad51 (1:2000, Thermo Fisher Scientific Cat# PA5-34905 ...
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bioRxiv - Plant Biology 2022Quote: ... The antibody used for immunoprecipitation was prebound with the equilibrated protein-G coated magnetic beads (Dynabeads, Thermo Fisher) for 3 h with gentle rotation at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... the adherens junction protein E-cadherin was stained using an anti-E-cadherin HECD-1 monoclonal antibody (Invitrogen) at 1:100 with subsequent incubation with the secondary DyLight594 conjugated anti-mouse antibody (1:100) ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-bound proteins were detected using SuperSignal™ West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific, Waltham, MA, # 34096) and imaged using a FluoroChem M (Protein Simple ...
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bioRxiv - Cell Biology 2019Quote: ... non-function blocking β1-integrin antibody (K20) was labelled using an Alexa Fluor 488 protein labelling kit (Invitrogen). Cells plated in the CSMA were then incubated live with the K20-Alexa Fluor 488 conjugated antibody for 1 h on ice to allow labelling of cell-surface integrins ...
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bioRxiv - Cell Biology 2020Quote: ... anti- HA-tag for KRAB protein (1:500, 32-6700 NOVEX HA epitope Tag antibody 5B1D10, Invitrogen, USA) and anti-b-Catenin (1:400 ...
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bioRxiv - Immunology 2020Quote: ... Anti-SARS-CoV-2 Coronavirus Spike protein (subunit 1) polyclonal antibody was purchased from Invitrogen (Carlsbad, CA, USA). Anti-SARS-CoV-2 Spike RBD rabbit polyclonal antibody was purchased from SinoBiological (Beijing ...
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bioRxiv - Microbiology 2021Quote: ... and anti-SARS-CoV-2 spike protein (RBD) polyclonal antibody (RBD, 1:1,000, Invitrogen cat. no. PA5-114451). All primary antibodies were diluted in PBST + 5% milk and applied to membranes for ≥16 hours at 4 °C ...
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bioRxiv - Genomics 2021Quote: ... 40 µg of Oct4 antibody (R&D, AF1759) was coupled to Dynabeads protein G (Thermo Fisher Scientific, 10009D) for 2 hours at room temperature for each sample ...
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bioRxiv - Genomics 2021Quote: Argonaute IPs were carried out using affinity purified rabbit antibodies pre-bound to Protein A Dynabeads (Fisher Scientific) (5-10 ug of antibody per 100 ul of beads ...
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mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5bioRxiv - Immunology 2022Quote: ... Final antibody concentrations were determined by measuring the A562 using Pierce™ BCA Protein Assay Kit (Thermo Scientific). Purified antibodies were stored at −80°C prior to use.
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bioRxiv - Neuroscience 2022Quote: Solubilisates were directly incubated with 7.5 µg Kv4.3 antibodies (Alomone, #APC-017) immobilized on Protein A Dynabeads (Invitrogen) for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Antibody-chromatin complexes were pulled-down using Protein A/G magnetic beads (Dy-10001D and Dy-10003D, Invitrogen), washed and then eluted ...
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bioRxiv - Cell Biology 2022Quote: ... The His SNX32ΔC protein bound to the lipids was detected by anti-His antibody [1:10,000 (Thermo Scientific)]
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bioRxiv - Neuroscience 2022Quote: ... 50 μg/ml of GPC3core protein was mixed with Alexa568-conjugated anti-hIgG antibody (Thermo Fisher Scientific, A21090) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were detected by incubation with secondary antibodies Alexa 568 donkey anti-mouse (Life Technologies, A10037, RRID: AB_11180865) diluted 1:1000 in blocking solution ...
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bioRxiv - Molecular Biology 2023Quote: ... we verified that the transformants express a protein recognized by an anti-V5 antibody conjugated with HRP (Invitrogen Cat #R96125 ...
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bioRxiv - Cancer Biology 2023Quote: Antibody internalization was assessed using the pHrodo™ iFL Red Microscale Protein Labeling Kit (Cat. #P36014, Thermo Fisher) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Beads that were made specific for DEAP2 by attaching the antibody to Protein G-coupled beads (Dynabeads, ThermoFisher) according to protocol ...
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bioRxiv - Biophysics 2023Quote: ... 80 pg of Npm2 antibody were cross-linked to 330 pl of Protein A beads (10001D, ThermoFisher Scientific). Interphase Xenopus egg extract containing cycloheximide was prepared as described above and immunodepletions were performed in a cold room ...
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bioRxiv - Microbiology 2023Quote: ... immunoprecipitation of Hfq-RNA complexes with an anti-Flag primary antibody using DynabeadsTM Protein A (Thermo Fisher, 10002D) overnight at 4 ℃ ...
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bioRxiv - Plant Biology 2023Quote: ... Immunoprecipitation was done using 1 µg of anti-GFP antibody (MBL) bound to Dynabeads protein A (Thermo Fisher). After washing and elution ...
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bioRxiv - Plant Biology 2024Quote: ... The EIN2-C native antibody was prebound with the equilibrated protein-G coated magnetic beads (Dynabeads, Thermo Fisher) with 1% BSA for 3 h with gentle rotation at 4 °C and incubated at 4 °C after cleared cell fractionation addition with gentle rocking overnight for Co-IP ...
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bioRxiv - Neuroscience 2024Quote: ... The presence of the Cas9 protein was tested using antibody against the V5 tag (ref R960-25, Invitrogen). Secondary antibodies used for Western blot were labelled with HRP (Jackson Immuno Research) ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were detected by horseradish peroxidase-conjugated secondary antibody using SuperSignal West Pico PLUS chemiluminescent substrate (ThermoFisher Scientific) and imaged with an Invitrogen iBright FL1500 imaging system (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysate was immunoprecipitated using the indicated antibodies conjugated to Dynabeads™ Protein G (Cat # 10003D, ThermoFisher Scientific). Immunoprecipitated proteins were run on SDS-PAGE gels and evaluated by Western blot as described below.
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bioRxiv - Molecular Biology 2024Quote: ... FLAG M2 or HA-tag antibody at 4 °C overnight before precipitated with magnetic Protein G beads (Thermofisher) for 2 hours at 4 °C followed by 3 washes with RIPA buffer.
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bioRxiv - Molecular Biology 2024Quote: ... RT with rotation) with endogenous CHD4 that was immobilized on 25 µL antibody-conjugated protein G dynabeads (Invitrogen) upon immunoprecipitation from whole cell lysates ...
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bioRxiv - Microbiology 2024Quote: ... or 2 μg of Flag Tag antibody (mouse) conjugated with Dynabeads Protein G Magnetic Beads (Invitrogen, 10004D, USA), or anti-HA magnetic Beads (Abmart ...
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bioRxiv - Cancer Biology 2024Quote: ... or with Protein A agarose beads incubated with 2 μg anti-LONP1 antibody (ThermoFisher Scientific 15440-1-AP) overnight (16 hours ...
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bioRxiv - Immunology 2020Quote: ... Precipitated proteins were bound to 50:50 Protein A: Protein G Dynabeads (Invitrogen) for one to two hours and washed extensively with IP wash buffer (50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were incubated with fast-flow protein G- or protein A-Dynabeads (Invitrogen) for 2 hours and centrifuged to eliminate nonspecifically bound proteins ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 50 mM NaCl using a 3.5 kDa MWCO SnakeSkin Dialysis Tube (Thermo Fisher Scientific, Waltham, Massachusetts). Next ...
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bioRxiv - Immunology 2019Quote: ... 40 mM NaCl) in a 20 kDa MWCO slide-a-lyzer dialysis units (Thermo Fisher Scientific) to remove methyl α-D-mannopyranoside and to prepare the sample for concentration by water evaporation (Savant DNA120 SpeedVac ...
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bioRxiv - Genetics 2022Quote: ... 1 mM DTT) using a Slide-A-Lyzer G2 Dialysis Cassette (10 kDa cutoff, ThermoFisher Scientific), and quantified with the BCA Protein Assay Kit (Pierce ...
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bioRxiv - Bioengineering 2022Quote: ... the solution was purified with a 7 KDa spin desalting column (ThermoFisher Scientific; Waltham, MA, USA) to obtain tetrazine-functionalized protein G (PGTz) ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.3 µl of 5 µg/µl 10-kDa dextran conjugated with Alexa Fluor™ 488 (Invitrogen, D22910 ...
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bioRxiv - Neuroscience 2021Quote: ... The dye mixture consisted of the calcium-indicator Fura-2 dextran (10,000 kDa, Life technologies, France) and of tetramethylrhodamine dextran (10,000 kDa ...
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bioRxiv - Immunology 2020Quote: ... the top fraction was passed through a 100 kDa (Cat # 88503 Pierce Thermo Scientific Waltham, MA) filter and its immunosuppressive ability was tested as before.
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bioRxiv - Biochemistry 2019Quote: ... 1.5 mM NaN3 using a slide-A-lyzer dialysis cassette (12-14 kDa MWCO, Thermo Scientific) at 4 °C for 15 h ...
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bioRxiv - Cell Biology 2020Quote: ... A single tail vein injection of 50 µl Dextran Texas Red (70 kDa, Thermo Fisher, D1864) in saline was used to label the brain vasculature in anesthetized animals ...
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bioRxiv - Evolutionary Biology 2021Quote: HEK293T cells57 were transiently transfected using 25 kDa branched polyethyleneimine and cultured in DMEM media (Invitrogen) supplemented with 2% (v/v ...
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bioRxiv - Plant Biology 2022Quote: ... BrBT-SCS: Pooled fractions were dialyzed using (20 kDa MWCO) Slide-A-Lyzer (Thermo Fisher Scientific), in 50mM Tris-HCl (pH 8 ...
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bioRxiv - Biophysics 2022Quote: ... the respective solutions were dialysed in Slide-A-Lyzer dialysis cassettes (2 kDa MWCO, Thermo Scientific) over night at 4 °C in 300 mM glycine ...
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bioRxiv - Biophysics 2022Quote: ... The resulting solution was dialyzed in a 30 kDa Slide-A-Lyzer (Thermo Fisher, Waltham, MA) at 4 °C for 2 hours against 50 ml of purified water to remove DMSO and then centrifuged for 15 min at top speed in an Eppendorf centrifuge at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... tohether with dextran beads fluorescently labeled with Oregon Green (OGDB; 70 kDa; ThermoFisher cat. no. D7173) at 16 h post-hCG ...
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bioRxiv - Bioengineering 2022Quote: ... The excess fluorophore was then removed using exosome spin columns (molecular weight cutoff 3 kDa, ThermoFisher). MSC-Exo were then reconstituted in PBS at a concentration of 1011 exosomes/ml ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM DTT using ‘SnakeSkin’ dialysis tubing with a 7 kDa molecular weight cut-off (ThermoFisher). His6-tagged cleavage products as well as TEV protease were removed with Ni-NTA agarose resin (Qiagen ...