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Citations for Thermo Fisher :
901 - 950 of 3080 citations for AKAP3 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... DNA transient transfection and ASCT2-siRNA (ID#s12916; Ambion) transfection were performed by using Lipofectamine 3000 following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... CCDC47 siRNA s32577 (all Silencer Select; ThermoFisher Scientific, USA).
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bioRxiv - Molecular Biology 2024Quote: ... siRNA transfections were carried out using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... or non-specific negative control siRNAs (ThermoFisher Scientific; 4390847) were purchased and resuspended in RNase-free H2O to generate a 25μM stock concentration ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA oligo transfections were performed using RNAiMax (ThermoFisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Astrocytes were transfected with siRNA using Lipofectamine 3000 (Invitrogen). After 48 hours of incubation ...
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bioRxiv - Cell Biology 2024Quote: ... or negative siRNA (Thermo Fisher Scientific, Silencer Select, #4390843) using siLenFect lipid reagent (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... siRNAs were delivered into cells using Lipofectamine RNAiMAX (ThermoFisher).
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with siRNAs using Lipofectamine RNAiMax (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... SiRNA transfection was performed with Lipofectamine RNAiMAX (ThermoFisher, 13778150) in Opti-MEM medium (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: siRNAs were transfected using the Lipofectamine RNAiMAX (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... RILPL1 and KIF5B siRNA using Lipofectamine RNAiMAX (Thermo Fisher) transfection reagent ...
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Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availabilitybioRxiv - Cell Biology 2023Quote: siRNA and sgRNA transfections were performed using RNAiMax (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... or a negative control siRNA (#4390846) (Thermo Fisher Scientific) was transfected into AtT-20 cells (2.0× 105 cells/well ...
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bioRxiv - Cell Biology 2024Quote: ... Gene knockdown by siRNA was performed using RNAiMAX (Invitrogen) according to the manufacturer!s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... the following siRNA constructs were purchased from Life Technologies: control siRNA (4390843) ...
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bioRxiv - Cell Biology 2024Quote: siRNA transfections were performed using Lipofectamine RNAiMax (ThermoFisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... All siRNAs were of Silencer™ Select grade (Ambion) to minimize off-targeting ...
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bioRxiv - Cancer Biology 2024Quote: ... siRNA transfection was performed using Lipofectamine RNAi MAX (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... were plated in six-well plates and transfected with CD44 siRNA for 24 h in antibiotic-free media using siRNA Lipofectamine RNAiMAX and OPTI-MEM I reduced serum medium (Invitrogen, Carlsbad, CA, USA), in accordance with the siRNA manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out in AML12 cells in a 12-well or 6-well plate or four-well chambered slides or 24-well Seahorse XF plate using 30 nM of the above indicated siRNAs and negative control siRNA with Lipofectamine RNAiMAX (Invitrogen; Life Technologies Inc.) following the reverse transfection protocol ...
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bioRxiv - Neuroscience 2021Quote: Subconfluent astrocyte cultures were treated with a sequence of Stealth siRNA duplex oligo ribonucleotides (siRNA Px1, 0.2 μM, ThermoFisher Scientific, catalog number 1330001) targeting rat Px1 mRNA and preventing Px1-mediated dye uptake (Garré et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were transfected after 24h with 30pmol concentration of siRNA (Integrated DNA Technologies) or scrambled control (Mission siRNA Universal Negative Control) using Lipofectamine® RNAiMAX Reagent (Invitrogen/Life Technologies) in Gibco™ OptiMEM ...
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bioRxiv - Immunology 2021Quote: Differentiating monocytes at day 2 of culture were transfected with two different MyD88 Silencer Select Validated siRNA or with a control siRNA (all at 50 nM final concentration; Ambion, Thermo Fisher Scientific) using Opti-MEM I reduced serum medium and Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... circRNA_33702 siRNA (100 nM), circRNA_33702 plasmid, WISP1 siRNA (100 nM), or negative control (Ruibo, Guangzhou, China) using Lipofectamine 2000 (Life Technologies, Carlsbad, CA, USA). Twenty-four hours after transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were also transfected with an siRNA directed against murine Meox1 (or a negative control siRNA) using Lipofectamine™ RNAiMAX Transfection Reagent (ThermoFisher Scientific 13778030) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were also transfected with an siRNA directed against murine Meox1 (or a negative control siRNA) using Lipofectamine™ RNAiMAX Transfection Reagent (ThermoFisher Scientific 13778030) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The screen used reverse transfection of siRNAs in which all wells had 250 nM siRNA concentration in 10 µL of Opti-MEM (Gibco; Thermo Fisher Scientific). Lipofectamine2000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then subjected to siRNA transfection with 10nM final concentration of control or target siRNAs using Lipofectamine™ RNAiMAX (Thermo Fisher Scientific), according with manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells in 35-mm dishes were transfected with 100 pmol of each siRNA in the Stealth siRNA library targeting 154 human nuclear proteins (Invitrogen–Thermo Fisher Scientific) using Lipofectamine RNAiMax (Invitrogen–Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: siRNA sequences were Dharmacon SMART siGenome on-target plus set of 4 duplexes and scrambled siRNA negative control from Thermo Scientific (Lafayette, CO). Tpp1 targeting siRNAs were (1-GGGCUGAGUUUCAUCACUAUU ...
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bioRxiv - Molecular Biology 2021Quote: ... Either MFGE8 siRNA or negative control siRNA was added into the cells and transfected using Lipofectamine 3000 Kit (Thermo Fisher, Waltham, MA, USA). The inhibitory efficiency of siRNA was detected by qRT-PCR.
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bioRxiv - Cell Biology 2022Quote: ... KIF5B (5’-CUACAUGAACUUACGGUU-3’) and non-targeting control siRNA (Silencer Select Negative Control No.1 siRNA Cat #4390843) were purchased from Ambion (Thermo Fisher Scientific). Cells were seeded in six-well plates at 30% confluence and transfected with 12.5-25 nM final siRNA concentration using Lipofectamine RNAiMax (Life Technologies ...
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Long Noncoding RNA MALAT1 is Dynamically Regulated in Leader Cells during Collective Cancer InvasionbioRxiv - Cancer Biology 2022Quote: ... UCA1 siRNA (Silencer® Select, Catalog #: 4392420) and control siRNA (Silencer® Select Negative Control No. 1 siRNA, Catalog #: 4390843) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Physiology 2022Quote: ... After 48 hours cells were transfected with siRNA against Tmem117 or siRNA control (30pmol/well) with lipofectamine RNAiMAX (Thermo Fisher Scientific, Cat# 13778100). 48 hours after transfection cells were trypsinized and pelleted by mild centrifugation (1200rpm for 3min) ...
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bioRxiv - Neuroscience 2023Quote: ... trigeminal ganglion neurons (TGs) were transfected with EGFP and Invitrogen Stealth RNAi™ siRNA Negative Control (scramble siRNA) (cat. no. 12935300, Thermo Fisher Scientific) or CRMP2 siRNA (5′ GTAAACTCCTTCCTCGTGT-3′ ...
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bioRxiv - Bioengineering 2023Quote: siRNA against Phactr3 or mock scramble siRNA at 4nM was prepared in Opti-MEM® I Reduced Serum Medium (Life Technologies 51985-026) with 16ul Lipofectamine® RNAiMAX Transfection Reagent (Life Technologies 13778075)/ml solution and incubating the solution at room temperature for 20mins ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa cells (in 100-mm dishes) were transfected with 500 pmol SAP30BP siRNA or negative control siRNA (Nippon gene) using Lipofectamine RNAiMAX (Invitrogen–Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were transfected with 25nM of gene-targeting ON-TARGETplus SMARTpool siRNAs or non-targeting pool siRNAs as negative control (Dharmacon) using the RNAiMAX reagent (Life Technologies; Cat#: 13778075) on two consecutive days ...
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bioRxiv - Biochemistry 2019Quote: ... by the addition of a 50 nM pre-designed siRNA primer pair CCAGGAAAUUAGCAAGGGATT (sense) and UCCCUUGCUAAUUUCCUGGGT (antisense) (Ambion/Thermo Fisher Scientific; siRNA ID# s15794) previously co-incubated for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... Silencer®Select siRNA targeting Mcl-1 (s8583) or Silencer®Select siRNAs targeting CAD (s4060, s223407) were transfected using Lipofectamine®RNAiMAX (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2021Quote: ... washed in PBS and taken up in 1M nucleofection buffer containing 11ug the pre-assembled Cas9 sgRNA RNP complex and were indicated 20 fmol of siRNA against TP53 (ThermoFisher #4390825, siRNA id s605). After nucleofection ...
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bioRxiv - Physiology 2024Quote: ... myotubes were transfected with 10 nM of either Silencer Select siRNA Negative Control No. 2 (no. 4390847) or Silencer Select siRNA s15542 targeting NR4A3 (Life Technologies, Foster City, CA). Transfections were performed for 5 h in OptiMEM reduced serum media with Lipofectamine RNAiMAX transfection reagent (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: GFP expressing Jurkat cells were cultured on porous (200 nm and 400 nm pores) and non-porous surfaces 30 min before incubation with siRNA (Silencer™ GFP siRNA from Invitrogen, 10025764, 5 nmol). Negative Control siRNA from the kit was used as a negative control ...
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bioRxiv - Neuroscience 2024Quote: FMRP knockdown was performed using a silencing RNA (siRNA) cocktail made with equal parts of three Silencer Select siRNA against the fmr1 gene (Thermo Scientific: s5315, s5316, s5317)25 ...
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bioRxiv - Cancer Biology 2021Quote: ... each siRNA was diluted in Opti-MEM medium (Invitrogen, 31985062) to a concentration of 10 µM ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were transfected with siRNA) using Lipofectamine RNAiMAX (Invitrogen) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: VPS51 knockdown was performed using stealth siRNA from Life Technologies (# 10620312-353281 D10 ...
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bioRxiv - Cell Biology 2020Quote: ... 200nM of siRNA was transfected into cells using oligofectamine (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... siRNAs were transfected with siImporter in OptiMEM medium (Life Technologies) containing 30 ng/mL M-CSF and 50 ng/mL RANKL as described (Touaitahuata et al. ...