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Citations for Thermo Fisher :
901 - 950 of 4948 citations for 9 Trimethylsilyl 6 trimethylsilyl oxy 9H purine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen Corp., USA) was used for counterstaining ...
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bioRxiv - Biochemistry 2024Quote: ... NHS-5/6-carboxyfluorescein (NHS-fluorescein, Thermo Fisher 46410), NHS-Oregon Green (Thermo Fisher O6147) ...
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bioRxiv - Cell Biology 2024Quote: ... human IL-6 Uncoated ELISA (88-7066-88, Invitrogen) and human GRO alpha (CXCL1 ...
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bioRxiv - Cell Biology 2024Quote: ... 4’,6-diamiino-2-phenylindole (DAPI; Invitrogen, Cat# D21490) diluted 1:1000 in PBS was applied for 15 minutes at room temperature with plates subsequently washed ...
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bioRxiv - Cell Biology 2023Quote: ... Culture medium was changed to Essential 6 (E6, GIBCO) medium supplemented with 6 μM CHIR99021 (R&D systems) ...
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bioRxiv - Immunology 2024Quote: ... under the selection of blasticidin (6 µg/mL, Gibco). 293T/17 (293T ...
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bioRxiv - Microbiology 2024Quote: ... 6% w/v phytoagar (Fisher Scientific, Waltham, MA, USA), pH adjusted to 5.8 ...
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bioRxiv - Microbiology 2024Quote: ... and 6 µL 2-mercaptoethanol (Fisher Chemical, Fisher Scientific) were added to a Qiagen powerbead tube ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated in 2 μM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, cat no: T3168) at 37°C for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 2.105 cells were seeded in 6-well plates and transfected with 20 nmol of siRNA and 9 μl of Lipofectamine RNAi Max according to the manufacturer’s protocol (ThermoFisher Scientific). A scramble siRNA (siMock ...
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bioRxiv - Cell Biology 2020Quote: ... PS in 45:35:15 molar ratio in 40 µl methanol:chloroform (9:1 v/v) were added into each well of a PolySorp 96-well plate (Thermo Fisher). All samples were performed in triplicate ...
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bioRxiv - Molecular Biology 2021Quote: ... by passaging at least 9 times in Dulbecco’s Modified Eagle Medium (DMEM) for SILAC lacking lysine and arginine (Thermo Scientific) containing 10 % dialyzed fetal bovine serum (Thermo Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... were sorted directly on a FACS Aria III in 9 μl of Specific Target Amplification (STA) reaction mix from the CellsDirect One-Step qRT-PCR kit (Invitrogen) containing 0.2XTaqMan Gene Expression Assay mix ...
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bioRxiv - Biochemistry 2019Quote: ... 5% [v/v] glycerol) was concentrated to 9 mg/mL (measured by UV absorbance in a NanoDrop spectrophotometer (Thermo Scientific) using the calculated molecular weight and estimated extinction coefficient ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... Neurons were transfected at 7-9 days in vitro (DIV) using Lipofectamine 2000 following the manufacturer’s directions (Thermo Fisher Scientific). A total of 1 μg of DNA was transfected for each well of a 12-well plate for 2-3 hours before switching back to conditioned media ...
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bioRxiv - Developmental Biology 2020Quote: ... Pelleted cell mixtures were resuspended in 10 μl of 1:9 collagen/setting buffer (10× Earle’s Balanced Salt Solution (Life Technologies), 0.2 M NaHCO3 and 50 mM NaOH) ...
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bioRxiv - Immunology 2021Quote: ... Cells were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Molecular Biology 2020Quote: ... were transfected with 9 μg of the appropriate hairpin in a pLKO-backbone vector using 30 μl of Lipofectamine 2000 (Invitrogen). After 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... and gene specific primer pairs (Supplementary Table 9) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative mRNA expression levels were calculated using the 2−ΔΔCt method and RPL18A as the house-keeping gene.
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bioRxiv - Bioengineering 2020Quote: ... 1 mg of fusion protein was dialyzed in 0.1 M NaCO3 pH 9 then mixed with 25 μg of FITC (ACROS organics) in a total volume of 0.5 mL ...
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bioRxiv - Cancer Biology 2020Quote: 293FT were used to prepare all lentiviruses and retroviruses and were maintained at 37°C in 9% CO2-containing humidified incubators using DMEM (Gibco) containing 10% fetal bovine serum (Hyclone ...
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bioRxiv - Microbiology 2020Quote: ... T4 phage were added (3×108 phages ml-1) to the AFM bio-cell together with SYTO 9 and PI (BacLight Bacterial Viability and Counting Kit, Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and fragmented for 9 min at 94°C in 2X Superscript III first-strand buffer containing 10mM DTT (Invitrogen, #P2325). The 10 μl of fragmented RNA was added to 0.5 μl of Random primers (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: PC-9 and HCC827 cell lines were cultured at 37C and 5% CO2 in RPMI 1640 GlutaMAX media (Gibco, US) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... Flp-In-293 cells were transfected with a 9:1 ratio of pOG44:pcDNA5/FRT-PRPF4 plasmids using Lipofectamine 2000 (Invitrogen) and 48 h post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... and loaded onto a 10% gelatin gel (9-10 µL for apical, 18-20 µL for basal, Novex, Life Technologies). Samples were electrophoresed for ∼90 min at 125-140V ...
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bioRxiv - Neuroscience 2022Quote: ... from each sample was labeled with 9 different TMT-10plex tags accordingly to the manufacturer’s protocols (cat# 90110, Thermo Scientific). The same mass tag and sample assignment was maintained throughout the entire study (old brain samples were labeled with TMT10-126 ...
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bioRxiv - Plant Biology 2022Quote: ... 9 RNA samples were extracted using TRIzol(tm) and RNA Purification Kit following the manufacturer’s protocol (ThermoFisher, Waltham, MA, USA). RNA was further purified using the TURBO DNA-free(tm ...
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bioRxiv - Neuroscience 2022Quote: ... was dissolved in 0.9% sterile Saline and pH was adjusted to 7.4 with 20-30 µl 1 N NaOH (#SS266-1, Fisher Scientific) according to Tocris Bioscience’s guidelines ...
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bioRxiv - Bioengineering 2022Quote: ... and samples were centrifuged at 8000xg for 5 minutes at −9°C in a Sorvall Legend Micro 17R (Thermo Scientific). The supernatant (extract ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was isolated from 9 µl lysate and 1 µl of ERCC 1:1000 Spike in control (Thermo Fisher Scientific) per sample using RNA SPRI beads (Agencourt ...
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bioRxiv - Biochemistry 2022Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in FreeStyle 293 Expression Medium (GIBCO). Plasmids were transiently transfected using Turbo293 (SpeedBiosystems ...
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bioRxiv - Microbiology 2020Quote: ... Israel) were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Molecular Biology 2020Quote: ... and pcDNA5/FRT/TO-based plasmids carrying the GFP-RIF1-L or GFP-RIF1-L-pp1bs gene were mixed in 9:1 molar ratio and used for transfection of Flp-In T-Rex 293 cells (Invitrogen) with Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... BSF RNAi cell lines were cultured in HMI-9 medium 47 supplemented with 10% (v/v) low-tetracycline foetal calf serum (FCS; Gibco) at 37 °C in 5% C02 and maintained in 5 µg.mL-1 Hygromycin (InvivoGen ...
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bioRxiv - Genetics 2020Quote: TaqMan copy number assays for CYP2D6 (assay IDs Hs04083572_cn and Hs00010001_cn for intron 2 and exon 9 respectively, Thermo Fisher Scientific) were run according to the manufacturer’s protocol on a ViiA7 Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... 9 μL of total RNA samples were proceeded to cDNA synthesis using High Capacity cDNA Reverse Transcription kit (Applied Biosystems). Then ...
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bioRxiv - Immunology 2022Quote: Human bronchial epithelial cells (BEAS-2B cells, ATCC® CRL-9609™) were cultured in LHC-9 medium (Life Technologies) and human lung fibroblast (MRC-5 cells ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were cultured in DMEM with 10% FBS and 1% P/S and were bulked up to 9 x T175 flasks of each by splitting and reseeding during their growth phase (∼80% confluence) using TrypLE (Gibco). The 9 x T175 flasks were seeded at ∼30% confluence in serum-containing media ...
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bioRxiv - Biochemistry 2022Quote: ... Baculoviruses for expression of the DNMT1 were generated in Spodoptera frugiperda 9 (Sf9) cells according to the Bac-to-Bac system instruction (Invitrogen). The protein expression was performed by infection of the baculoviruses with the Sf9 cells for 72 hrs at 27 °C ...
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bioRxiv - Cell Biology 2019Quote: ... the dishes were coated with 1 ml of gelatin in PBS (1: 9 of 0.1% fluorescein isothiocyante (FITC)-gelatin (# G13186, Invitrogen, MA): 2% porcine gelatin) ...
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bioRxiv - Molecular Biology 2019Quote: Phage proteins were isolated from a purified phage lysate (10^9 pfu/ml) by a single methanol/chloroform extraction (1:1:0.75, v/v/v) (Acros Organics) and precipitated by addition of an equal volume of methanol (14,000 x g ...
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bioRxiv - Systems Biology 2021Quote: ... day 9 spheroids were dissociated and stained for 30 minutes with Image-iT Lipid Peroxidation Sensor C11-BODIPY (Thermo Fisher) at a final concentration of 10 μM according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Per2Luc mice and littermate wild-type control mice were euthanized by isoflurane 2-3 hours before lights off (ZT 9–ZT10) and tissues were immediately removed into ice-cold HBSS (Gibco). Coronal brain slices containing SCN (400-µm thickness ...
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bioRxiv - Immunology 2020Quote: ... TIB-47) were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Microbiology 2021Quote: ... 20 µg of total RNA was reverse transcribed using 9 ng of oligo(dT)21 and 15 ng of random octamers (Invitrogen) in the presence of Cy3 or Cy5-dCTP (Invitrogen ...
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bioRxiv - Synthetic Biology 2020Quote: ... transfection complexes containing 3 μL of Xtremegene-9 reagent and 500 ng of plasmid DNA were prepared in 100 μL of OptiMEM (Invitrogen). After 20 min of incubation at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Monocytes were then differentiated into macrophages by 7-9 days of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Immunology 2021Quote: ... The 96-well plates w preloaded with 9 μL of RT-STA solution: 5 μL of CellsDirect PCR mix (Invitrogen), 0.2 μL of SuperScript-III RT/Platinum Taq mix (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We subsequently placed two frozen individuals from each family and treatment group (n = 9 families * 2 treatments (+/− supplemental thiamine) * 2 individuals = 36) into 10 volumes of RNAlater-ICE (Invitrogen) pre-chilled to −80 °C and allowed the samples to reach −20 °C overnight ...