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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for 4 Chloro 6 fluorobenzene 1 3 diamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... We incubated overnight with the anti-GFP primary antibody at 4 degrees (1:1000, A-6455 Invitrogen, 0.1M PBS 0.3% tx100, 3% NGS). After abundant PBS washes ...
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bioRxiv - Genomics 2022Quote: ... Plasmids were mixed in a 3:1 weight ratio of total plasmid DNA:PEI in 4 mL of either OptiMeM™ (Gibco cat. # 31985-062) or serum-free DMEM per flask and then shaken vigorously for 30 sec ...
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bioRxiv - Microbiology 2020Quote: ... Cells were infected in a BSL-3 lab with the UF-1 strain of SARS-CoV-2 at MOI of 4 in media containing 3% low IgG FBS (Fisher Scientific, Cat. SH30070.03).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and lentiviral vector expressing GFP-UMOD-WT or GFP-UMOD-H177- R185 del at a ratio of 3:1:4 using Lipofectamine 3000 Reagent (ThermoFisher Scientific, Waltham, MA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed 3 times with 200 µL PBS for 4 min before secondary antibodies and 1 µg/mL Hoechst (Thermo Fisher Scientific #33342) were added in 35 µL per well and incubated for 1 hour at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... protein fractions were mixed with SDS-free loading buffer in a 3:1 ratio and loaded on a Novex™ 4–20% Tris-Glycine Gels using NativeMark™ Unstained Protein Standards (Invitrogen™) (range from 20 to 1236 kDa ...
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bioRxiv - Cancer Biology 2024Quote: ... and the packaging plasmids psPAX2 and pMD2.G at a ratio 4:3:1 in the presence of Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). The supernatants containing lentiviral particles were collected 48 hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated at room-temperature for 3-4 hours with secondary antibody mixture containing Alexa Fluor 568 anti-mouse (1:500; Thermo Fisher Scientific, A11031) and Alexa Fluor 647 anti-chicken (1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-6 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Reverse 5′-CGA AGG TGT GAC TTC CAT G-3′) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
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bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-6 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
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bioRxiv - Neuroscience 2022Quote: ... the cerebral cortexes from 2-3 P3-P5 C57BL/6 mice were collected in ice cold HBSS (Invitrogen), the tissue was washed three times with HBSS and digested with 0.04% trypsin (Sigma ...
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bioRxiv - Cell Biology 2022Quote: HCT116 cells were cultured in a 6-well plate at 300,000 cells per well in 3 ml McCoy’s 5A medium (Gibco) supplemented with 10% heat inactivated FBS and with 90 UI/mL Penicillin ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 30 ovaries from 3-6 day-old flies using TRIzol (Thermo Fisher Scientific) in three replicates ...
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bioRxiv - Cell Biology 2023Quote: ... X-biotin-PE [N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine] was obtained from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... antibodies overnight at 4°C and then incubated for an additional 3 hours at 4°C with 50 μL DynabeadsTM protein G (Life Technologies, 10004D). Following three washes with 1% Triton-TBS lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... Fungal hyphae were stained with 0.5 mM 4.4-difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (C1-BODIPY 500/510 C12, Thermo Fisher Scientific) or 4.4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16 ...
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bioRxiv - Cell Biology 2020Quote: ... 6 or 9 µl of 20 µM siRNA and 4 µl of Lipofectamine RNAi MAX (13778150, Invitrogen) were suspended in 100 µl of water (W4502 ...
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bioRxiv - Cell Biology 2020Quote: ... then stained for one minute with 30 ng/mL 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, D1306) in 1x PHEM ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue nuclei were visualized with nuclear stain 4′,6-diamidino-2-phenylindole (DAPI, 62247; Thermo Fisher Scientific). Coverslips were mounted using Prolong Gold (P36934 ...
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Prom1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epitheliumbioRxiv - Cell Biology 2022Quote: ... 4′,6-diamidino-2-phenylindole (DAPI) was used to stain intracellular DNA (NucBlue, Thermo Fisher, Waltham, MA). Alexa Fluor 647 Phalloidin (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... Sections were stained with DAPI (4′,6′-diamidino-2-phenylindole) and mounted in Prolong Gold (Life Technologies). Images were obtained by confocal microscopy (Nikon C2+ Eclipse ...
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bioRxiv - Cell Biology 2021Quote: ... then stained for one minute with 30 ng/mL 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen D1306) in 1x PHEM ...
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bioRxiv - Neuroscience 2020Quote: ... the slides were incubated in 1μg/ml of 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) at RT for 10min ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were mounted with ProLong Diamond Antifade Mountant with 4′,6-diamidino-2-phenylindole (DAPI; Life Technologies) and imaged using a Leica SP5 DMI (zebrafish sections ...
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bioRxiv - Cell Biology 2021Quote: ... 4′,6-diamidino-2-phenylindole (DAPI) (cat#D-1306) and TRIzolTM (cat#15596018) were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2020Quote: ... The nuclei were stained with 300 nM DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific, Slovakia) for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... Cover glasses were mounted on slides with mountant with 4’,6-diamidino-2-phenylindole (DAPI) (Life Technologies) and imaged with a confocal microscope.
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bioRxiv - Bioengineering 2022Quote: ... cells were stained with antibodies and 500ng/mL 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI, Invitrogen # D1306). Samples were analyzed on an LSR II flow cytometer (BD).
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bioRxiv - Cell Biology 2022Quote: ... 6 or 9 μl of 20 μM siRNA and 4 μl of lipofectamine RNAi MAX (Invitrogen, 13778150) were suspended in 100 μl of water (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI) staining was used to visualize the nuclei (Thermo Fisher Scientific). Fluorescence was assessed using a fluorescence laser microscope (LSM780 ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 × 105 cells were seeded in 6-well plates and cultured with RPMI 1640 medium (ThermoFisher # 11875093) containing 10% FBS (Seradigm) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lines were passaged every 4-6 days and MIN6 were detached with 0.25% trypsin-EDTA (Gibco) and seeded at 7.5-1×106 cells per 75 cm2 flask ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Alexa Fluor™ 488 (#C10337) and 4’,6-diamidino-2-phenylindole (DAPI; #62248) were purchased from Invitrogen-ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... The nucleus was stained with 50 ng/ml 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen Cat. #D1306) for 10 min at room temperature ...
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bioRxiv - Physiology 2020Quote: ... 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI) staining was used to visualize the nuclei (Thermo Fisher Scientific). Fluorescence was assessed using a fluorescence laser microscope (LSM780 ...
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bioRxiv - Cell Biology 2022Quote: ... Organoids were passaged every 4 to 6 days by dispersion with TrypLE Express (Invitrogen, Carlsbad, CA, USA) and re-embedded in Matrigel-GFR.
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bioRxiv - Cancer Biology 2024Quote: ... The sections were mounted with 4’,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher, Waltham, MA, United States) before imaging and we examined more than five different microscopic images (per section ...
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bioRxiv - Bioengineering 2024Quote: ... This was followed by foregut cell differentiation from day 4 - 6 using advanced DMEM/F12 (Gibco; 12634) with 2% B27 (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... sections were counterstained with 0.2 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Cat# 62248) diluted in 1xPBS for 15-30 min at RT ...
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bioRxiv - Plant Biology 2023Quote: ... 250 ng/ml 4′,6-diamidino-2′-phenylindole dihydrochloride (DAPI) or 500 nM SYTOX Orange (Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... After another set of gentle washes and 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining (Thermo Scientific, R37606 ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were visualized with 4′,6-diamidino-2-phenylindole (DAPI) in SlowFade Diamond antifade mounting medium (ThermoFisher). Primary antibodies were incubated with tissues overnight at 4°C in 1% normal donkey serum ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei staining was performed with 4’,6 diamidino-2-phenylindole dihydrochloride (DAPI, Molecular Probes, Eugene, OR, USA) at 300 nM in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 0.02 mg/mL, ThermoFisher Scientific, USA), mounted and cover-slipped before being imaged with the Zeiss Axio Imager.Z1 Microscope ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride) NucBlue (Cat. #: R37606; Thermo Fisher Scientific). Slides were mounted using Aqua-Poly/Mount (Cat ...
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bioRxiv - Biochemistry 2023Quote: ... and 2mM DTT) for 30 min at 4 °C and analyzed on 6% TBE gels (Life Technologies) at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... Cell nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng/ml; Molecular Probes). Stained sections were imaged using epifluorescence and deconvolution epifluorescence microscopy on an Olympus IX83 ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA samples were then treated with 4-6 units of Turbo DNase (Thermo Fisher Scientific, cat# AM2238) at 37 °C for 30 min to remove the bulk of plasmid DNA contamination ...