Labshake search
Citations for Thermo Fisher :
9401 - 9450 of 10000+ citations for QuantiChrom Indole Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... at final concentration of 10 µM for 4 hours and then harvest to perform Click-iT reaction using Click-iT EdU flow cytometry Alexa Fluor 488 assay kit (Life technologies, Carlsbad, CA, USA, C10420). Fluorescence measurements were taken using LSRfortessa X-20 (BD Biosciences) ...
-
bioRxiv - Immunology 2022Quote: In situ-hybridization on lungs was performed as previously described (71) using the ViewRNA™ ISH Tissue Assay Kit (Invitrogen by Thermo Fisher Scientific, Darmstadt, Germany) according to the manufacturer’s instructions with minor adjustments ...
-
bioRxiv - Cancer Biology 2022Quote: ... 10μM EdU was added to the culture media for 2h prior to fixation and detection using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific, C10424), according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... the bacterial community activity was assessed by measuring the protein concentration using Thermo Scientific™ Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA) and the ammonia concentration using Sigma-Aldrich® Ammonia Assay Kit (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2022Quote: ... The identity and concentration of the purified protein was examined and quantified using the sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and the Pierce Bradford Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).
-
bioRxiv - Systems Biology 2022Quote: ... The concentration of the glucose produced from the glycogen was determined using the Amplex Red Glucose/Glucose Oxidase Assay kit (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions.
-
bioRxiv - Genomics 2022Quote: The DNA content of the libraries were quantified using a Qubit™ ssDNA Assay Kit on a Qubit™ 3 Fluorometer (Thermo Fisher Scientific, 2017), then pooled in equal DNA proportions ...
-
bioRxiv - Cell Biology 2020Quote: ... or specific Taqman microRNA assay primers with a TaqMan® MicroRNA Reverse Transcription Kit for the assessment of microRNAs (4366596, Applied Biosystems™, Thermo Fisher). Downstream qPCR amplifications of first-strand cDNA were performed using TaqMan® Universal PCR Master Mix ...
-
bioRxiv - Immunology 2019Quote: The concentration of sPD-1 and sTim-3 was measured in thawed plasma samples by enzyme linked immunosorbent assay (ELISA) using Human PD-1 (PDCD1) ELISA kit [EHPDCD1] (Thermo Fisher Scientific, Waltham, MA USA) and Quantikine ELISA Human TIM-3 Immunoassay kit [DTIM30] (R&D Systems ...
-
bioRxiv - Pathology 2020Quote: ... approximately 50 ng of RNA was used in a previously published qRT-PCR duplex assay (Price et al. 2010) with the TaqMan® RNA-to-Ct™ 1-step kit (Applied Biosystems™, ThermoFisher Scientific) on a QuantStudio™ 3 Real-Time PCR system (Applied Biosystems™ ...
-
bioRxiv - Plant Biology 2020Quote: Young seedlings were incubated in 20 μM 5-ethynyl-2’-deoxyuridine (EdU) (Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit, ThermoFisher Scientific/Invitrogen, Waltham, Massachusetts, USA) made in ddH2O for 30 min at 24 °C ...
-
bioRxiv - Microbiology 2021Quote: ... The extracted DNA yield (ng DNA g-1 dry weight soil) was quantified with the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). We recognize that this calculation does not take into account inflation in DNA yield due to the growth of fungi and other organisms not accounted for in our study ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... DNA was first quality-checked by gel electrophoresis and then quantified with a PicoGreen fluorometer quantitator and Quant-iT™ PicoGreen™ dsDNA Assay Kit (Invitrogen™, ThermoFisher Scientific). The Genomic Sequencing and Analysis Facility (GSAF ...
-
bioRxiv - Immunology 2020Quote: ISH was performed as reported previously (Erickson et al., 2020; Osterrieder et al., 2020) using the ViewRNA™ ISH Tissue Assay Kit (Invitrogen by Thermo Fisher Scientific) following the manufacturer’s instructions with minor adjustments ...
-
bioRxiv - Genomics 2021Quote: ... Total RNA concentration was measured using Qubit® RNA HS Assay Kit on a Qubit® 2.0 Fluorometer (Thermo Fisher Scientific Inc., Waltham, MA, USA). Integrity was assessed using Agilent RNA 6000 Nano Kit on a 2100 Bioanalyzer instrument (Agilent Technologies ...
-
bioRxiv - Immunology 2022Quote: We isolated T cells from PBMC of patients and healthy controls for autologous mixed lymphocyte reaction (MLR) assay using Dynabeads Untouched Human CD4+ T Cells kit (Invitrogen Dynal cat. No.113.52D, US). For MLR ...
-
bioRxiv - Microbiology 2022Quote: ... Extracts were clarified by centrifugation (13,000 × g for 10 minutes) and quantified by Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). 500 μg of cell lysate were combined with 5μg of IP antibody and incubated overnight at 4°C with mixing to form the immune complex ...
-
bioRxiv - Molecular Biology 2022Quote: ... The supernatant was transferred to a new tube and protein concentrations were assessed using a bicinchoninic acid (BCA) assay kit (Thermo Fisher Scientific; Waltham, MA, USA) and microplate spectrophotometer (Synergy H1 ...
-
bioRxiv - Plant Biology 2023Quote: ... A 100 μL plant extract was used in each reaction and the H2O2 concentration was measured with the Amplex Red hydrogen peroxide/peroxidase assay kit (Thermo Fisher Scientific, Cat. No. A22188).
-
bioRxiv - Immunology 2024Quote: ... The total protein concentrations in amniotic fluid (supernatant) and tissue lysate samples were tested by the Pierce BCA Protein Assay Kit (Cat# 23225; Pierce Biotechnology, Thermo-Fisher Scientific, Inc., Rockford, IL, USA) prior to immunoblotting ...
-
bioRxiv - Microbiology 2024Quote: ... The concentration and purity of extracted DNA from all samples and the controls were evaluated using Invitrogen™ Quant-iT™ dsDNA Assay Kit (Thermo Fisher Scientific, USA) and a Nanodrop 2000 (Thermo Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... 0.5 g of each microbial mat sample and checked the concentration and quality of the DNA extracts using the Qubit dsDNA BR Assay kit (Thermo Fisher Scientific, Waltham, MA, USA). We used the Nextera XT kit (Illumina ...
-
bioRxiv - Neuroscience 2023Quote: ... All the OCR and ECAR values were normalised by DNA content measured for each well using the Quant-iT™ PicoGreen™ dsDNA Assay Kit (Invitrogen™, Dublin, Ireland) as previously described by [106].
-
bioRxiv - Molecular Biology 2023Quote: ... the cDNA was used for real-time PCR with three multiplex wells once with TaqMan gene assay kit (Thermo Fisher Scientific, Applied Biosystems, Carlsbad, CA, USA). The results were normalized to GAPDH ...
-
bioRxiv - Immunology 2023Quote: The viral load in different tissue samples was determined as described previously (72) TaqMan™ 2019nCoV Assay Kit v1 (Thermo Fisher Scientific, Waltham, MA, USA).
-
ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: Candidate SNP genotyping was performed using TaqMan® Genotyping Assay kit on ABI 7500 Real-Time PCR System from Applied Biosystems (Foster City, CA, USA). Each polymerase chain reaction sample was composed of 10 ng of DNA ...
-
bioRxiv - Biophysics 2023Quote: ... we determined the total protein concentration of each sample using a Pierce™ Microplate BCA Protein Assay Kit (Thermo Fisher Scientific, 23252, Waltham, MA, USA) to calculate the normalized collagen content.
-
bioRxiv - Molecular Biology 2023Quote: ... The resultant sediment was dissolved in 40 µl of PBS and its protein concentration was estimated by a bicinchoninic acid (BCA) assay kit (23228, 1859078, Thermo Fisher Scientific, Waltham, MA, USA), using bovine serum albumin (23209 ...
-
bioRxiv - Molecular Biology 2023Quote: RNA-protein pull-down assay (RNAP) was conducted in triplicates following a modified protocol adapted from Pierce™ Magnetic RNA-Protein Pull-Down Kit (Thermo Scientific™, No.: 20164). Briefly ...
-
bioRxiv - Physiology 2023Quote: OPP fluorometry was performed adapting the regular instructions for the Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit (cat #C10456; Thermo Scientific, Waltham, MA) to live myofibers ...
-
bioRxiv - Biophysics 2023Quote: ... Total protein concentrations of tissue and cancer cell samples were determined using the Pierce BCA Protein Assay Kit (#RG235622, Thermo Fisher Scientific, Waltham, MA, USA). Further steps were performed as described for protein isolation from mouse organ tissues.
-
bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were prepared from 10 ng gDNA using the Ion Torrent™ Oncomine™ Comprehensive Assay v3C (OCAv3C) kit (Thermo Fisher Scientific, cat# A35806) and the Ion Torrent™ Ion Chef™ instrument model 4247 (Thermo Fisher Scientific ...
-
Identification of ABHD6 as a regulator of lysophosphatidylserines in the mammalian liver and kidneysbioRxiv - Biochemistry 2024Quote: ... The protein concentrations of the membrane and soluble proteomic fractions were measured using the Pierce BCA Protein Assay Kits (Thermo Fisher Scientific, catalog no: 23225).
-
Identification of ABHD6 as a regulator of lysophosphatidylserines in the mammalian liver and kidneysbioRxiv - Biochemistry 2024Quote: ... The protein concentrations of the membrane and soluble proteomic fractions were measured using the Pierce BCA Protein Assay Kits (Thermo Fisher Scientific, catalog no: 23225).
-
bioRxiv - Microbiology 2024Quote: ... according to the manufacturer’s instructions and DNA concentration in extracts was measured with Qubit™ dsDNA High Sensitivity (HS) assay kits and Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham Massachusetts, US). RNA was extracted using the RNeasy kit (Qiagen ...
-
bioRxiv - Molecular Biology 2024Quote: Nascent protein synthesis was analyzed in HPMs by using the Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (Invitrogen™, cat #C10428). Click-iT® HPG (L-homopropargylglycine ...
-
bioRxiv - Molecular Biology 2020Quote: Global translation assay was performed using Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay (Thermo Fisher Scientific). Briefly ...
-
bioRxiv - Genetics 2020Quote: ... 12.5 μL of TaqMan Gene Expression Master Mix and 1.25 μL of primers and probes (TaqMan® gene expression assay, assay ID: Rasa3, MM00436272.M1; Applied Biosystems). Samples were prepared in triplicate ...
-
bioRxiv - Bioengineering 2021Quote: ... qRT-PCR assays were performed as TaqMan single-plex assays on the StepOne Plus Real-Time PCR System (Thermo Fisher Scientific), using the manufacturer recommended settings for quantitative and relative expression ...
-
bioRxiv - Biochemistry 2020Quote: ... the purified LC/A variants were subjected to BCA assay to quantify protein concentration through comparison to BSA standards (Pierce™ BCA Assay, ThermoFisher). The proteins were stored at 4 °C before assays.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: The copy number variations (CNVs) in the CYP2D6 gene were determined with the TaqMan® Copy Number Assay (Assay ID: Hs00010001_cn; Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2022Quote: ... The soluble supernatant was collected and protein concentration was quantified via bicinchoninic assay (BCA) assay (Pierce Thermo Scientific, Rockford, IL, USA). Normalized soluble supernatant or 20 μL of conditioned media was run on a 4-20% Tris-Gly SDS-PAGE gel (Life Technologies ...
-
bioRxiv - Physiology 2021Quote: ... The supernatant was collected and protein concentration was quantified via a bicinchoninic assay (BCA) assay (Pierce Thermo Scientific, Rockford, IL, USA). Approximately 30 μg of soluble supernatant was run on a 4-20% Tris-Gly SDS PAGE gel (Life Technologies ...
-
bioRxiv - Systems Biology 2019Quote: ... and protein concentration in each sample was determined by Bradford assay using Pierce™ Coomassie Plus Assay Reagent (Thermo Fisher Scientific). Subsequently ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: The assay procedure is based on the manufacturer’s protocol for LanthaScreen™ TR-FRET Thyroid Receptor beta Coactivator Assay (Invitrogen, PV4686) with slight ...
-
bioRxiv - Genetics 2021Quote: ... and DNA pulled down using an anti-AHR antibody from melanocytes heterozygous for rs117132860 (c87, c197 and c262) was used as template DNA for a Taqman genotyping assay (Assay ID: ANU699F, Thermo Scientific). All experiments were performed for at least three biological replicates.
-
bioRxiv - Molecular Biology 2024Quote: ... proteins were quantified with the Bradford Assay following a standard protocol (Coomassie Plus—The Better Bradford Assay™ Reagent, Thermo Scientific), and equal amounts of solubilized proteins were heated in Laemmli sample buffer (Bio-Rad ...
-
bioRxiv - Microbiology 2024Quote: ... gene for the internal normalization primers-probe (VIC) set (VIC-MGB: assay ID Hs03405707_g1; TaqMan Gene Expression Assay, Thermo Fisher Scientific). Briefly ...
-
bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen, Waltham, MA, USA). 5 µg of each protein fraction was added to 1x loading buffer (250 mM Tris pH 6.8 ...
-
bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR was performed using Applied Biosystems custom TaqMan Gene Expression Assays designed using the Taqman Assay Search Tool (Life Technologies) for the ubiquitous CDC42 isoform ...