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Citations for Thermo Fisher :
9201 - 9250 of 10000+ citations for Recombinant Human GC Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Peptide sequences and hence protein identities were determined by matching protein databases with the acquired fragmentation pattern by the software program Sequest (Thermo Fisher Scientific)65 ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting supernatant was used for protein concentration determination using a Pierce 660 nm Protein Assay (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Neuroscience 2024Quote: ... The protein concentrations in the cytoplasmic and nuclear fractions were determined using a Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Cat. 23228). The isolated cytoplasmic and nuclear proteins were aliquoted and stored at -80°C for subsequent analysis.
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bioRxiv - Microbiology 2023Quote: ... then protein concentration was determined using the Pierce™ BCA Protein Assay Kit as per manufacturer’s instructions (cat:23225, Thermo Scientific™). Samples were then processed using the S-trap protocol as per the manufacturer’s instructions54 ...
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bioRxiv - Microbiology 2023Quote: Proteins on the surface of Env-expressing HEK293T cells were biotinylated with the Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... A total of 20 µg of protein lysate was loaded onto 4- 12% Bis-Tris Plus Mini protein gels (ThermoFisher, Cat # NW04122BOX) and transferred onto nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: Protein concentration in venom and antivenom samples was measured using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... and run in a Bio-Rad Mini-PROTEAN Tetra Vertical Electrophoresis Cell at 50 mA for approximately 45 minutes alongside a prestained protein ladder (PageRuler Prestained Protein Ladder, 10 to 180 kDa, Thermo Fisher Scientific). Gels were stained for 1 h in a Coomassie solution (0.1% Coomassie brilliant blue R-250 ...
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bioRxiv - Biochemistry 2023Quote: ... Insoluble fraction was removed by 4°C centrifugation at 12000xg for 10 minutes and protein concentration in the soluble fraction was measured by PierceTM BCA protein assay kit (Thermo Fisher Scientific). Protein concentration was adjusted to 1-2 mg/mL for LDBIA treatment.
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were measured using bicinchoninic acid assay to measure protein concentrations and equal amounts of proteins were mixed with Nupage LDS sample buffer (NP0007, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Lysates were then cleared by centrifugation and protein concentrations determined by Bradford Assay using a Peirce Protein Assay Kit (Thermo Scientific 23200). Equal amounts of protein were denatured in reducing sample buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein lysates (10 μg/lane) were fractionated on a NuPAGE Novex 4%–12% Bis-Tris Protein Gel (Thermo Fisher Scientific) and transferred onto a PVDF membrane (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... The lysate was cleared by centrifugation (18,000 x g for 10 min at 25°C) and the protein content of the supernatant was determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific). Reduction ...
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bioRxiv - Neuroscience 2023Quote: ... was recombinantly expressed as an N-terminal Fc-fusion protein (Fc-hTfR1) in Expi293 suspension cells and purified by gravity flow with PierceTM Protein A Agarose (Thermo Fisher Scientific). HFCII probes were loaded with Fc-hTfR1 by thresholding to a 1 nm shift ...
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bioRxiv - Plant Biology 2024Quote: ... protein concentrations of fractions containing FtsZ1-2 and FtsZ2-1 were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... Protein content of the crude mitochondrial fraction was measured by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific catalog number 23225). Thirty µg of mitochondria protein was incubated at 25 °C in 850 µL reaction buffer (100 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were incubated in rotation at 4°C for 20 hours with a protein complex of anti-GFP antibody (MBL) and Dynabeads Protein A (Thermo Fisher Scientific). After the rotation ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were cleared by centrifugation and diluted to equal total protein concentrations following analysis using Pierce BCA protein assay kit (Thermo Fisher Scientific). Prior to loading on gels ...
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bioRxiv - Cell Biology 2024Quote: ... the bound proteins were eluted by boiling the beads in protein loading buffer and resolved on NuPAGE 4–12% Bis-Tris protein gels (Thermo Fisher Scientific) followed by immunoblot analysis (see above).
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bioRxiv - Microbiology 2024Quote: The total proteins from the peritoneal cells and foot tissue samples were extracted using a protein extraction kit (Thermo Fisher Scientific, USA). The targeted proteins were separated by 10% or 15% SDS‒PAGE based on the molecular weight and then bonded to 0.22 μm PVDF membranes ...
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bioRxiv - Cell Biology 2024Quote: ... bath sonicated for 5 minutes and centrifuged at 13000g for 10 minutes at room temperature prior to quantification of total protein by a Pierce 660 Protein Assay (Thermo Scientific #22660). Condensates were obtained by centrifuging at 10,000xg for 10 minutes and resuspended in 5%SDS in 50 mM TEAB prior to total protein quantification ...
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bioRxiv - Molecular Biology 2024Quote: The protein concentrations of both fractions were determined in duplicate on the same day as protein isolations to minimize freeze-thaw effects using a commercially available assay (bicinchoninic acid [BCA] Protein Assay Kit; Thermo Fisher Scientific). Thereafter ...
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bioRxiv - Cell Biology 2024Quote: ... and then normalized to both the internal standard (d5-2HG) peak area and protein content of duplicate samples as determined by BCA protein assay (Thermo Fisher Scientific). Ions used for quantification of metabolite levels are as follows ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentrations of the lysed sEV preparations were determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA), according to the provided guidelines ...
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bioRxiv - Molecular Biology 2024Quote: ... We measured the total protein content in the prepared seeds by BCA quantification (Micro BCA™ Protein Assay Kit, Thermo Fisher Scientific). 2% (w/w ...
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bioRxiv - Biochemistry 2024Quote: The cell surface expression of wild-type and mutant hGPR30-HA was analysed via cell surface protein isolation using a Cell Surface Protein Isolation Kit (#89881, Thermo Scientific™) followed by western blotting ...
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bioRxiv - Bioengineering 2024Quote: ... Protein lysates were then electrophoresed on a NuPAGE Bis-Tris Protein Gel (4-12%) using NuPAGE SDS Running Buffer (Thermo Fisher Scientific) for 2 hr at 130 V before transfer to a nitrocellulose membrane in Towbin Buffer (20 mM Tris-HCl pH 8.3 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and then normalized to the concentration of total proteins assessed by using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... Cell debris were pelleted by centrifugation for 20 min at 4 °C and 20,000 x g and the total protein concentration of the cleared lysate was determined using the Pierce Rapid Gold BCA Protein Assay Kit (Thermo Scientific, #A53225) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: RNA-protein interactions were detected using Pierce RNA 3’ End Desthiobiotinylation Kit and Pierce Magnetic RNA-Protein Pull-Down Kit (Thermo Scientific, #20163), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... The membrane proteins of liver non-parenchymal cells (NPCs) were purified using the Mem-PER Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific)
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bioRxiv - Neuroscience 2024Quote: ... Total protein concentration of Triton X-100 soluble and insoluble fraction was determined using BCA Protein Assay Kit (Thermo Fisher Scientific Inc).
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations of both the cytosolic and membrane fractions were measured using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) and equal amounts of protein were loaded on to a NuPAGE 4-12% Bis-Tris gel (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... 200μg of protein was used for click chemistry and was performed using biotin-alkyne and protein reaction buffer kits (Thermo Fisher: C10276). AHA-biotin-alkyne labeled proteins were pulled down with streptavidin beads and the indicated proteins were analyzed by immunoblotting.
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then incubated with 20 μg of recombinant glutathione S-transferase (GST)-RBD-MICAL-L1 and Pierce™ Glutathione magnetic agarose beads (Life Technologies-Invitrogen, cat# 78601) for 2 hrs at 4°C ...
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bioRxiv - Immunology 2024Quote: All CoMiX were generated from HEK293T cells transfected with four μg of recombinant plasmid DNA using Lipofectamine-3000 (Invitrogen, Thermo Fisher Scientific BVBA, Merelbeke, Belgium) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: The VHH M13 phage-display library was subjected to two rounds of affinity enrichment (panning) against immobilized recombinant OspA on Nunc-Immunotubes™ (Thermo Fisher Scientific, Waltham, MA) using protocols described elsewhere (56) ...
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bioRxiv - Molecular Biology 2023Quote: ... this latter was performed on 1 ng of genomic DNA (gDNA) of each organism listen in Table 2 by using the Taq DNA polymerase recombinant (Invitrogen™, Carlsbad, 254 CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... and protein digested with Proteinase K (ThermoFisher Scientific, 25530049). Samples were purified with DNA Clean and Concentrator columns (Ozyme ...
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bioRxiv - Cancer Biology 2021Quote: ... Rabbit anti-Myelin Basic Protein (BMP) (ThermoFisher; 1:300), Mouse anti-NeuN (Abcam ...
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bioRxiv - Systems Biology 2020Quote: ... Protein concentrations were analyzed with BCA assay (Thermo Scientific Pierce BCA Protein Assay Kit ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 μl Dynabeads Protein G (Thermo Fisher Scientific, 10004D), and 400 μl dilution buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... protein concentration was measured by BCA assay (Thermo Scientific), and protein concentrations were equalized prior to boiling in the recommended sample buffers supplemented with 2.5% beta-mercaptoethanol (Bio-Rad) ...
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bioRxiv - Molecular Biology 2019Quote: ... protein A and G Beads (10002D, 10003D; Life Technologies) were first blocked with 5mg/ml BSA (A7906 ...
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bioRxiv - Cancer Biology 2021Quote: ... BCA protein assay kit (Thermo Fisher Scientific, MA, USA) was used measuring protein concentration per manufacturer instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by binding to Protein-G Dynabeads (Invitrogen, 10003D) for 20 minutes hours at 4°C to enrich for endothelial cell nuclei ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were blotted on nitrocellulose membrane (Fisher scientific). The membrane was blocked in 5% milk/TBS-T for 1 h followed by overnight incubation with primary antibody at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... and 20 µL Protein G DynaBeads slurry (Invitrogen 10004D) overnight at 4 °C in an end-over-end mixer ...
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bioRxiv - Cell Biology 2020Quote: ... and 20 µL Protein G DynaBeads slurry (Invitrogen 10004D) overnight at 4 °C in an end-over-end mixer ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was transferred to a nitrocellulose membrane (ThermoFisher, LC2000) and then blocked with Odyssey Blocking Buffer (Li-Cor Biosciences ...