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Citations for Thermo Fisher :
9101 - 9150 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and 1:1 3-methyl-1-butanol (Thermo Fisher Scientific) in mineral oil were used as cues ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1% antibiotics in DMEM/F12 (1:1) media (Gibco).
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bioRxiv - Immunology 2022Quote: ... Zonula occludens-1 (ZO-1, 1:250 Invitrogen 33-9100), and Cav-1 (1:500 Invitrogen PA5-17447) ...
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bioRxiv - Neuroscience 2024Quote: ... cells were switched to neural induction media (DMEM/F12 and Neurobasal medium at 1:1 ratio, 1% B27, 0.5% N2, 1% PenStrep, 1% Glutamax; ThermoFisher Scientific) supplemented with ascorbic acid (0.1 mM ...
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bioRxiv - Developmental Biology 2021Quote: ... Generation of cDNA from 1 µg of RNA was performed using a High-Capacity RNA-to-cDNA Kit (Thermo Fisher Scientific, Burlington, ON, Canada). Real-Time PCR was performed using FastStart SYBR Green Master (Roche Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... and subject to qRT-PCR assays by the Power SYBR® Green RNA-to-Ct™ 1-Step Kit (Thermo Fisher Scientific, Waltham, MA) to measure molecule expression levels ...
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bioRxiv - Genomics 2020Quote: ... and subsequently subjected into nested polymerase chain reactions (PCR) according to manufacturer’s instructions prescribed in HIV-1 genotyping kit: Amplification module (Applied Biosystems, Life Technologies, Warrington, UK). The amplified cDNA was purified using ExoSAP-IT™ PCR product clean-up reagent (Applied Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... and 5 μl was used in a 20 μl PCR reaction with 1 μl of each 10 μM primer and 10 μl of VeriQuest SyBr with Fluorescein kit (Affymetrix, Santa Clara, CA, USA). For each reaction ...
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bioRxiv - Cancer Biology 2019Quote: ... Total RNA samples (1 μg) were used to perform a reverse transcription reaction with a Maxima First Strand cDNA Synthesis kit (Thermo Scientific, cat. no. K1671) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Reverse transcription reactions were performed on 1 µg of DNase-treated RNA using the RETROScript Reverse Transcription Kit (Ambion, AM1710, Thermo Fisher, Waltham, MA) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... The number of JEV RNA copies present in BBB-crossing samples was determined by RT-qPCR using TaqMan® Fast Virus 1-Step Master Mix kit (Applied Biosystems®, 4444432) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Cell-free DNA extractions from 1-4 ml of plasma were performed with MagMAX Cell-Free DNA Isolation Kit (Thermo Fisher Scientific, Austin, TX) or the QIAmp Circulating Nucleic Acid Kit (Qiagen ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was extracted using TRIzol® reagent and a Qiagen miRNeasy kit and treated with 1 µl DNase I (Thermo Fisher Scientific, #AM2238) per 10 μg of RNA.
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bioRxiv - Cell Biology 2021Quote: ... cDNA was synthesized from 1 μg of total RNA using an Invitrogen two-step kit with SuperScript™ III (#18080051, Invitrogen, Carlsbad, CA, USA) as the reverse transcriptase (RT ...
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bioRxiv - Microbiology 2021Quote: ... MHV-A59 was quantified by RT-qPCR by amplifying a fraction of its ORF5 gene for the structural protein M.15,16 The TaqMan™ Fast Virus 1-Step Master Mix Kit (Thermo Fisher Scientific Inc., 4444432) was used for RT-qPCR ...
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bioRxiv - Biochemistry 2020Quote: ... coli PepP coding sequence was PCR-amplified (primers 1 and 2) from BW25113 genomic DNA prepared using a GeneJET Genomic DNA Purification Kit (Thermo Scientific, Cat No. K0721) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... from 3 individual (1 young and 2 old) mice and mRNA was extracted with the Dynabeads™ mRNA DIRECT™ Purification Kit (61011 Thermo Fisher Scientific). Reverse transcription was performed with the Maxima H Minus Reverse Transkriptase (EP 0751 Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... 300 µL of PW1 solution from DNeasy PowerWater Kit was mixed in and 30 µL of Proteinase K (20 mg mL-1, Fisher Scientific, Cat. no. AM2546) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesized from 1 µg total RNA template with oligo-dT primers using a SuperScript® III First-Strand Synthesis kit (ThermoFisher Scientific, 18080-051) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: RNA was extracted from the 47 tumor samples and reverse transcription was performed on 1 µg of RNA from tumorous or healthy tissue using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and the reaction of RNA (1 μg) reverse transcription to c-DNA using High-Capacity kit (Applied Biosystems, Foster City, CA, USA Boston, USA), following the protocol described by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: Cardiomyocytes were isolated from the left ventricle of 1–2-day-old Sprague-Dawley rats following the protocol from an isolation kit (Cellutron Life Technologies, cat#nc-6031). Myocytes were then plated on cover slips coated with laminin (Life tech #23017-015 ...
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bioRxiv - Bioengineering 2022Quote: ... dissociated cells were stained for an hour on ice with NCadherin antibody (1:200) conjugated in-house using an Alexa Fluor 647 conjugation kit (A32733, Thermo Fisher Scientific, MA, USA). The bottom 30% and top 10% of cells based on AF647 signal were then sorted to deplete or enrich for N-cadherin markers ...
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Telomerase deficiency in humans is associated with systemic age-related changes in energy metabolismbioRxiv - Cell Biology 2022Quote: ... 1ug of DNAse 1 treated RNA was reverse-transcribed into first-strand cDNA using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems; Thermo Fisher Scientific, Inc.). The primer sequences and thermal cycling parameters used to quantify fully spliced hTERT (primer set E4-E5 ...
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bioRxiv - Neuroscience 2022Quote: ... 10 µM EdU was administered for 1 h prior to fixation and detected using the Click-iT™ Plus EdU Alexa Fluor™ 555 Imaging Kit (ThermoFisher Scientific, C10638) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... Reverse transcription was performed on 1 μg of RNA using the High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Thermo Scientific, Hemel Hempstead, UK). All quantitative PCR experiments were conducted on an ABI 7900HT (Perkin-Elmer Applied Biosystems ...
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bioRxiv - Zoology 2019Quote: ... followed by qPCR analysis on a 7500 Real-Time PCR System with the Power SYBR® Green RNA-to-CT™ 1-Step kit (Applied Biosystems, USA) using primers listed in Table 1 ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 μg of RNA for GAS5 was used to synthesize cDNA using high Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) in a T-Professional Basic ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... followed by chloroform-methanol protein precipitation to obtain pure protein pellets that were subsequently resuspended in digestion buffer (100 mM TEAB with 1% (v/v) SDC) and quantified by the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, United States). From each sample ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were incubated for 8 minutes in TSA Plus Fluorescein solution (1/50 TSA Plus Fluorescein in 1x Amplification Buffer (Tyramide Signal Amplification Labeling Kit No. 2; Molecular Probes, Thermo Fisher Scientific)) in darkness ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was synthesized with 1 μg of total RNA using Maxima First Strand cDNA Synthesis Kit (K1672; Thermo fisher scientific, Grand Island, NY, USA). Real-time RT-qPCR for mRNA was performed using PowerUP SYBR Green master mix (Thermo fisher scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... and total plasma protein was quantified after a 1:600 dilution utilizing a Micro BCA™ Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA).
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bioRxiv - Cell Biology 2020Quote: ... A total of 1 µg of RNA were used for cDNA synthesis with the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Foster City, USA) in a thermal cycler (Veriti® Thermal Cycler ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used for flow cytometric analysis (Supp. Fig. 1): LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit (ThermoFisher Scientific, Cat. #L34967), CD45-Alexa Fluor 700 (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... The enriched cells were then subjected to magnetic bead separation (EasySep Mouse Streptavidin Rapidspheres Isolation Kit, Stem Cell, 19860) to remove the EpCAM+ cells (CD326 1:200, ThermoFisher Scientific, 13-5791-82). The EpCAM-depleted cells were stained with antibodies against CD45 and a viability dye ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA synthesis and PCR analysis was carried out from equal amount of total RNA (1 μg) from each sample utilizing One-Step kit (Invitrogen Life Technologies, Carlsbad, CA, USA). Primers for target gene were designed using OligoAnalyzer 3.1 software ...
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bioRxiv - Microbiology 2022Quote: ... two aliquots of each antibody (1 mg/mL) were labelled with biotin (EZ-Link Sulfo-NHS-LC-Biotinylation Kit, ThermoFisher Scientific, Waltham, MA, USA) for the capture position and SULFO-TAG (GOLD SULFO-TAG NHS-Ester ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 ng of RNA for each sample was retrotranscribed and amplified by Power SYBR® Green RNA-to-CT™ 1-Step Kit (Thermo Scientific, 4389986), using specific primers for EGFP ...
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bioRxiv - Microbiology 2022Quote: ... Products were visualized on 1% agarose gels and purified using a MagJET NGS Cleanup and Size Selection Kit (Thermo Fisher Scientific, Waltham, MA, USA). The resulting purified libraries were finally quantified using a Quantus fluorometer (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... The final expression vectors were subcloned into the pLATE11 bacterial expression vector via Ligation Independent Cloning (LIC, aLICator LIC Cloning and Expression Kit 1, untagged; Thermo Fisher Scientific, Waltham, USA) following the manufactures’ instructions ...
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bioRxiv - Genetics 2024Quote: ... according to the manufacturer’s instructions.1 μg of total RNA was reverse transcribed using the High-Capacity cDNA Archive Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions in 20 μl final volume at 37 °C for 2 hours following a pre-incubation at room temperature for 10 min ...
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bioRxiv - Immunology 2024Quote: ... CD45+ immune cells were used for scRNA sequencing and were prepared for FACS-sorting from endometrium as follows: Cells were first stained using the 150 µL 1:600 LIVE/DEADTM Fixable Aqua stain kit (Molecular Probes™; Cat. L34957) diluted in DPBS+/+ on ice for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... The amplified PCR product of the C-His oipA gene fragment was cloned into vector pLATE11 using aLICator LIC Cloning and Expression Kit 1 (Thermo Fisher Scientific, United States) by following the kit’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... stephensi midguts were transferred to a fresh drop of (1:400) 3SP2-Alexa488 anti-CSP antibodies (DyLight 488 Anitibody Labeling Kit, Thermo Fisher Scientific ref. 53024) and incubated for 30 minutes at RT in a slide humidity incubation box ...
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bioRxiv - Cell Biology 2023Quote: ... The PDMS was prepared by mixing thoroughly with curing kit at a ratio of 10:1 (wt%) and degassed in a desiccator (Fisher Scientific; #50-212-719) under vacuum system for 40 min ...
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bioRxiv - Cell Biology 2023Quote: ... and blotted with anti-6xHis antibody (1:5000 dilution) followed by HRP-labeled secondary antibody and ECL reagents from the Pierce Fast Western kit (Thermo Scientific, Waltham, MA, USA). The bottom half was stained with SimplyBlue Safe stain (Life Technology ...
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bioRxiv - Cell Biology 2023Quote: ... The concentration of isolated RNA was quantified using a NanoDrop® spectrophotometer and 1-2 µg was used for cDNA synthesis with a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™, 4368814). qPCR reactions were conducted using a 7500 Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Immunology 2022Quote: ... the tissue sections were washed twice for 2 minutes in wash buffer and then incubated with a 1:10 dilution of ELF97 phosphatase substrate (E6600, ELF™ immunohistochemistry kit, Invitrogen by Thermo Scientific) for 10-20 minutes ...
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bioRxiv - Microbiology 2023Quote: ... RNP complexes and ssODNs were delivered to Jurkat E6-1 cells using the Neon® Transfection System and Neon® Transfection 10uL Kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative reverse transcription (RT) and real-time PCR (qRT-PCR) were done using TaqMan™ RNA-to_Ct 1-Step kit (Thermo Fisher Scientific, AB 4392938) on BioRad CFX 96 Real-time systems with the following TaqManTM probes and primers purchased from Thermo Fisher Scientific ...