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Citations for Thermo Fisher :
9101 - 9150 of 10000+ citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin, Vector Laboratories, #L-1060 + DyLight755 Antibody Labelling Kit, Thermofisher Scientific, #84538) were injected in the mouse tail vein to label and visualize the human vessels ...
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bioRxiv - Microbiology 2021Quote: ... and 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530) were injected to label and visualize the human vessels ...
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bioRxiv - Microbiology 2021Quote: ... and the human vasculature with 100μg of Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin, Vector Laboratories, #L-1060 + DyLight755 Antibody Labelling Kit, Thermofisher Scientific, #84538). Neutrophils were labelled with 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G ...
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bioRxiv - Immunology 2022Quote: ... were transfected with plasmids carrying J08 and 02M04 antibody heavy and the light chains with a 1:2 ratio using ExpiFectamineTM293 Transfection Kit (Thermo Fisher Scientific) as recommended by the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... They were both determined to be of the IgG2a subclass by using the Pro-DetectTM Rapid Antibody Isotyping Kit-Rat (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... For immunoprecipitation of endogenous B55α, B55α antibody (clone 2G9, Milipore) was covalently cross-linked to agarose beads using Pierce Crssolink IP kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2019Quote: ... (Amgen Inc.) was covalently attached to 60mg of Dynabeads in a volume of 6mL using the Dynabead® Antibody Coupling Kit (Life Technologies), according to the manufactory’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... antibodies overnight at 4°C.The sections were then washed with PBS and incubated with a biotinylated secondary antibody for 15 min from a Histostain Plus kit (Invitrogen, CA, USA). The sections were then washed and incubated with 3 ...
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bioRxiv - Cell Biology 2019Quote: ... CALX173ACE solubilized CX3CL1 was loaded into magnetic beads previously crosslinked to polyclonal anti-CX3CL1 antibody using an IP kit (Pierce, Thermo Fisher Scientific). Retained CX3CL1 was eluted by pH shock (basic ...
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bioRxiv - Biochemistry 2021Quote: ... Capture antibodies were coupled to Carboxylated Paramagnetic Beads from the Simoa Homebrew Assay Development Kit (Quanterix) using EDC chemistry (Thermo Fisher Scientific). Detection antibodies were conjugated to biotin using EZ-Link NHS-PEG4 Biotin (Thermo Fisher Scientific) ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: The fluorescence-labeled AFP1-His proteins were prepared according to the manufacturer’s instruction of the DyLight 550 Antibody Labeling kit (Thermo Fisher; Cat#84530). One OD600 of cells washed with MES buffer was subjected to immunostaining using the primary anti-HA antibody and AF488-conjugated secondary antibody or wheat germ agglutinin conjugated antibody (WGA-A488 ...
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bioRxiv - Molecular Biology 2020Quote: ... at 48 h post transfection and immunoprecipitated using HA tag antibody as per the instructions given in the Pierce Co-Immunoprecipitation kit (Thermo Scientific, USA).
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bioRxiv - Molecular Biology 2020Quote: The immune precipitated samples using HA antibodies were resolved by SDS-PAGE and the gel was silver stained using Pierce Silver Stain Kit (Thermo Scientific, USA). The distinct protein bands were excised and were subjected to in-gel trypsin digestion using In-Gel Tryptic Digestion Kit (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... the antibodies were labeled with Alexa Fluor® 568 and Alexa Fluor® 488 respectively using the Alexa Fluor® Antibody Labeling Kits according to the manufacturer’s manual (Thermo Fisher). The antibodies for fluorescence labeling were dissolved in PBS without any glycerol.
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bioRxiv - Cell Biology 2022Quote: ... by immunoprecipitation in antibody (each 20 µg) immobilized agarose beads (each 50 µl) from Pierce™ Co-Immunoprecipitation Kit (Thermo Scientific™). For each assay ...
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bioRxiv - Immunology 2022Quote: ... with anti-His and anti-IL-15 antibodies and live/dead staining) using the LIVE/DEAD Fixable NearIR Dead cell stain kit (Thermo Fisher Scientific) for all flow cytometry analysis ...
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bioRxiv - Bioengineering 2024Quote: ... Capture antibodies (provided in Supplementary Table 1) were biotinylated using the EZ-Link micro Sulfo-NHS-biotinylation kit from (Thermo Fisher Scientific). The biotinylated antibodies were then diluted to 10 μg/mL in a 1 % BSA solution and added to the grids for 15 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... as previously described [63] and neutrophils were labelled by were labeled with 2.5 µg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530). Mice were infected by intravenous injection of 100 µl of the bacterial inoculum (107 CFU total ...
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bioRxiv - Immunology 2024Quote: The sandwich ELISA assay was performed with 4 µg/ml coated F(ab’)2 fragments that were generated from full-size antibodies using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Fisher, 44980) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... TNF-α and IL-5 cytokines using a mouse cytokine 11-plex antibody bead kit (Th1/Th2 Cytokine 11-Plex Mouse ProcartaPlex™ Panel, Cat No# EPX110-20820-901, Thermo Fisher). Preparation of samples ...
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bioRxiv - Cell Biology 2023Quote: ... Mono-biotinylated Fab’ fragments of goat anti-mouse Ig(G+M) were labeled with AF546 using an Alexa Fluor 546 antibody labeling kit (Thermo Fisher Scientific). The molar ratio of AF546 to Fab’ in the AF546-labeled Fab’ was ∼2 determined by a Nanodrop spectrophotometer (Nanodrop Technologies).
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bioRxiv - Molecular Biology 2023Quote: ... A cognate secondary antibody coupled to horseradish peroxidase was used and revealed with the Super Signal West Dura Extended Duration Substrate kit (Thermo Fisher Scientific). Acquisition was performed using the Fusion FX (Vilber ...
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bioRxiv - Cell Biology 2023Quote: ... We further purified the polyclonal antibodies using a PfSTART1 affinity column that was generated using the AminoLink® Plus Immobilisation Kit (Thermo Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... For quality controls, PSCs passed array CGH testing (Cell Line Genetics, Madison, WI) and pluripotency testing using a four-antibody kit (Thermo Fisher #A24881). Cell culture media were sterile filtered (Fisher Scientific #SCGP00525 and #0974063D) ...
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bioRxiv - Neuroscience 2019Quote: ... and subsequently incubated in secondary antibody (goat anti-chicken 488 1:200, Invitrogen A32931; goat anti-rabbit 647 1:200, Invitrogen A21244; goat anti-rabbit 568 1:200, Invitrogen A11011 ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated overnight at 4°C in a 5% milk solution containing a 1:1000 dilution of rabbit anti-DsbA antisera followed by incubation with a Goat anti-rabbit IgG HRP (ThermoFisher) secondary antibody at a dilution of 1:10,000 at room temperature for 30 minutes prior to development with a chemiluminescent kit (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... Furin bands were detected using rabbit anti-furin polyclonal at 1:1000 and Goat anti-rabbit HRP (Invitrogen, 1:5000) was used as secondary antibody.
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bioRxiv - Cell Biology 2019Quote: ... The parasites were subjected to incubation with secondary antibody (1:400 anti-rabbit Alexa 568, 1:400 anti-rabbit Alexa 488, 1:250 anti-mouse Alexa 594 from Invitrogen) for 1.5 hours followed by three washes with 1X PBS for 10 minutes each ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed again in PBS and incubated for 2 h at room temperature in secondary antibody in carrier solution (donkey anti-goat 647, 1:1000, ThermoFisher; donkey anti-rabbit 405, 1:1000, Abcam; donkey anti-rabbit 555, 1:1000, ThermoFisher). After four final washes ...
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bioRxiv - Pathology 2020Quote: ... Alexa Fluor 488 goat anti-rabbit IgG (A11008) and Alexa Fluor 555 goat anti-rabbit IgG (A21428) were purchased from Invitrogen Biotechnology Co. ...
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bioRxiv - Immunology 2021Quote: ... polyclonal rabbit anti-Banf1 (Cat. PA5-20329) and goat anti-rabbit IgG (H+L)-Alexa Fluor 647 were purchased from ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-ZO-2 pAb (38-9100) and rabbit anti-ZO-3 pAb (36-4100) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... HA or GFP-tagged DISP1 variants (anti-HA rabbit polyclonal, 1:1000 or anti-GFP rabbit polyclonal, 1:2000, Invitrogen), and β-actin (anti-β-actin rabbit polyclonal ...
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bioRxiv - Cell Biology 2022Quote: ... The next day the fibers were washed 3×10 min in PBS containing 0.04% saponin and incubated in blocking buffer with Alexa 488 anti rabbit or Alexa 568 anti rabbit or anti mouse (Invitrogen) for 2 hours ...
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bioRxiv - Physiology 2022Quote: ... Secondary antibodies used were mouse anti-rabbit (1:10’000, Jackson, 211-032-171) and rabbit anti-sheep (1:10’000, Invitrogen, 81-8620).
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bioRxiv - Neuroscience 2022Quote: ... Slices were then transferred to fresh 0.3% PBS-Triton and incubated overnight at 4°C with appropriate primary antibodies (1:200-500 dilution of goat anti-ChAT IgG, Millipore, AB114P; 1:500 rabbit anti-GFP IgG, Abcam, ab6556 or 1:300 rabbit anti-GFP IgG, ThermoFisher, A-6455 (both anti-GFP had similar level of expression)).
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bioRxiv - Cell Biology 2023Quote: ... Embryos were devitellinized by ethanol popping and stained with rabbit anti-phospho-ULK1 (Ser555) (1:50, Cell Signalling Technology) and goat anti-rabbit Alexa 488 (1:500, Invitrogen); and with phalloidin Alexa 647 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... To visualize Aurora kinase C, we used rabbit anti-mAurC (1:500, FORTIS) and Alexa-Fluor 568 donkey anti-rabbit IgG (1:200, Invitrogen). For H2AT120ph ...
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bioRxiv - Immunology 2024Quote: ... Secondary Abs (Donkey anti-goat alexafluor 594, Donkey anti-rabbit AlexaFluor 594, Donkey anti-rabbit AlexaFluor 488; Invitrogen; 1:1000) were incubated in PBT-BSA for 1hr at RT and 24 hrs at 4C.
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bioRxiv - Cancer Biology 2024Quote: ... 2 µg of Fc control and CLEC18A-Fc fusion were incubated with AlexaFluor647 rabbit anti-mouse and goat anti-rabbit IgG (Invitrogen) at a molar ratio of 4:2:1 in array PBS and preincubated on ice for 10 mins at a total volume of 65 µl ...
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bioRxiv - Neuroscience 2024Quote: ... secondaries (Donkey anti-rabbit Alexa Fluor Plus 488 [A32790], Donkey anti-rabbit Alexa Fluor Plus 647 [A32787]) and HOECSHT336 (ThermoFisher) were diluted in antibody diluent for 1h at RT ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibodies included Alexa546 goat anti-mouse (red) antibody (Molecular Probes) and Alexa488 goat anti-rabbit (green ...
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bioRxiv - Microbiology 2022Quote: ... as the primary antibody followed by Alexa647-conjugated secondary antibody (Invitrogen). The monolayers were imaged using a BioTek Cytation3 instrument and virus foci (approximately 100-200/well in no-serum control wells ...
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bioRxiv - Molecular Biology 2020Quote: ... The secondary antibodies were Alexa Fluor conjugated goat antibodies (Thermo Scientific): α-mouse ...
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bioRxiv - Genomics 2020Quote: ... Primary antibodies were mouse anti-MF20 antibody (1:100, 14650382, Invitrogen) and rabbit anti-TMSB4X antibody (1:200 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were then labeled using AlexaFluor-conjugated secondary antibodies (Invitrogen). Sections were visualized with a Zeiss Imager ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in antibody diluent (003118, Thermo Fisher Scientific) and sections were incubated overnight at 4°C in a humidified chamber ...
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bioRxiv - Biochemistry 2020Quote: The following antibodies were used: anti-V5 antibody (Invitrogen R960-25), mouse anti-FLAG M2 antibody (Sigma F1804) ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies (Alexa Fluor secondary antibodies, Thermo Fisher Scientific, Waltham, Massachusetts) were applied according to species specificity of primary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... The corresponding secondary antibodies included: 488 Donkey anti-mouse antibody (Invitrogen), 596 Goat anti-guinea-pig antibody (Invitrogen) ...