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Citations for Thermo Fisher :
9001 - 9050 of 10000+ citations for Fatty acid binding protein FABP1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2024Quote: ... Protein concentration in each extract was measured using PierceTM BCA Protein Assay Kit (Thermo Scientific). Laemmli buffer (Biorad ...
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bioRxiv - Microbiology 2024Quote: ... Total protein was quantified using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific, USA). Calprotectin was normalized to initial stool weight (ng calprotectin/mg stool).
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bioRxiv - Molecular Biology 2024Quote: ... protein was isolated using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific Inc, 78501), and Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific Inc ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific Inc, 23225), and 10 μg electrophoresed in 4–12% Bis-Tris denaturing gels (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were determined using BCA assay (23225, Pierce™ BCA Protein Assay Kit, Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... We combined 4 mg of Cas9 Nuclease protein (TrueCut Cas9 Protein V2, Thermo Fisher Scientific), 5 mL of Neon Buffer R (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing sgRNA targeting human hypoxanthine phosphoribosyltransferase 1 (HPRT1) (LentiArray™ CRISPR Positive Control Lentivirus, human HPRT, Thermo Fisher Scientific Cat # A32829). HPRT1 knockout cells shows resistance to 6-thioguanine (6TG ...
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bioRxiv - Immunology 2022Quote: ... The supernatants of human CD19 CAR T cells or human TCR transfected CD8+ T cells were analyzed by IFNγ Human Uncoated Elisa Kit (Invitrogen, Thermo Fisher Scientific # 88-7316-88). Plates were read on BioRad plate reader at a wavelength 450 nm and subtracted by 595 nm.
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bioRxiv - Molecular Biology 2022Quote: Female human embryonic kidney cells HEK-293FT and male human fibrosarcoma HT1080 cells were cultured in DMEM-GlutaMAX + Pyruvate (Life Technologies SAS, Saint-Aubin, France), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... the blots were probed for the presence of heavy and light chain bands using either horseradish peroxidase (HRP)-conjugated polyclonal goat anti-human IgG Fc antibody or polyclonal goat anti-human kappa chain antibody (Thermo Fisher Scientific, Waltham, MA, USA). Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Human promyelocytic leukemia (HL-60) cells and human umbilical vein endothelial cells (HUVECs) were grown in RPMI-1640 medium (Gibco, Thermo Scientific, MA, United States) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Dissolved protein was analyzed for protein concentration by micro-scale BCA assay (Micro BCA Protein Assay Kit #23225; Thermo Fisher Scientific Inc., Waltham, MA, USA). Lysates measured at 50 μg were separated using 10% sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE ...
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bioRxiv - Neuroscience 2021Quote: ... Intensities of specific bands were normalized to a protein loading control or to the total protein levels marked using MemCode™ Reversible Protein (Thermo Fisher Scientific, MA, USA).
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bioRxiv - Bioengineering 2021Quote: ... 16 Protein concentration of the extracts was estimated using a BCA protein assay kit (Pierce™ BCA Protein Assay Kit, ThermoFisher Scientific, Oakville, ON, Canada) as per supplier’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Intensities of specific bands were normalized to a protein loading control or to the total protein levels marked using MemCode™ Reversible Protein (Thermo Fisher Scientific, MA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin-immunoprecipitated DNA was analyzed by quantitative PCR with binding site-specific primers (Extended Data Table 2) using PowerUP SYBR green master mix (Thermo Fisher Scientific, Cat # A25743) and a 7900HT Fast Real-Time PCR machine (Applied Biosystems) ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were resuspended in nickel-binding buffer (50mM Tris-HCl, pH8.0, 1M NaCl, 1mM PMSF) and lysed using a sonicator (Fisher Scientific Sonic Dismembrator Model 500) at 35% power ...
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bioRxiv - Microbiology 2020Quote: Chimeric proteins were diluted to 1 μg/ml in carbonate buffer (pH 9.6) and used to coat polystyrene 96-well high-binding ELISA plates (100 μl/well; Nunc-Immuno plate with MaxiSorp surface). After overnight incubation at 4 °C ...
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bioRxiv - Immunology 2022Quote: The cells were first stained with FC block (CD16/32) to block nonspecific binding and then were stained with dead dye to exclude dead cells (Aqua, ThermoFisher or Zombie NIR, Biolegend) for 15 min at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: Analysis of heme binding for KD determination was performed by UV/Vis spectroscopy on a Multiskan GO microplate spectrophotometer (Thermo Fisher Scientific, Waltham, MA) as described earlier37 ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBD417, RBD501, RBD484 and RBDtrip) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBDmut) (K417N, E484K, N501Y) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Biochemistry 2022Quote: ... Affinities of αE2 and αDHED to ERs were measured using the LantaScreen TR-FRET competitive binding assay screening protocol by the SelectScreen Biochemical Nuclear Receptor Profiling Service (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2022Quote: ... the RBD was inserted into pcDNA3.4 with a streptavidin binding peptide (SBP) tag at the C-terminus and were produced using the Expi293 Expression System (Thermo Fisher Scientific, Waltham, MA) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... sections were blocked for endogenous peroxidases and non-specific binding before applying primary antibodies rabbit polyclonal anti-Gas6 (ThermoFisher, # PA5-79300, dilution 1:100) and rabbit polyclonal anti-Spp1 (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... Direct pepscan analysis was undertaken by binding 200 ng of each overlapping peptide to Nunc MaxiSorp 96 well ELISA plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... for RoNi/7.1 cells and 1% Non-Essential Amino Acids (NEAA; Thermo Fisher Scientific) for Huh-7 and RoNi/7.1 cells ...
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bioRxiv - Developmental Biology 2021Quote: ... and detected using the chemi-luminescent nucleic acid detection module (Pierce Biotechnology, ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... Then the sections on chips were stained with nucleic acid dye (Thermo fisher, Q10212) for ssDNA visualization ...
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bioRxiv - Molecular Biology 2021Quote: ... total nuclei acid content was measured spectroscopically at 260 nm by Nanodrop (Thermo Scientific) (1A260 = 50 ng/µl) ...
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bioRxiv - Microbiology 2021Quote: ... the chamber was precoated with 1 % poly L-glutamic acid (Fisher Scientific, P4886-25MG) for one hour and washed twice with 70 % EtOH ...
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bioRxiv - Developmental Biology 2020Quote: ... 1% of Penicillin-Streptomycin and 1% of MEM Non-Essential Amino Acids (Gibco, U.S.). Cells were passed in T25 flasks at 37 °C with 5% CO2.
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bioRxiv - Neuroscience 2022Quote: ... The membrane was exposed using the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher).
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bioRxiv - Neuroscience 2021Quote: ... neurons were anchored with succinimidyl ester of 6-((Acryloyl)amino) hexanoic acid (AcX) (Thermofisher) in PBS (0.1mg/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... Non-essential amino acid and L-glutamine was purchased from Invitrogen (Thermo Fisher Scientific) and Polyethylenimine was purchased from Polysciences ...
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bioRxiv - Molecular Biology 2021Quote: ... The gels were subsequently soaked in SYBR Gold nucleic acid stains (ThermoFisher, Cat.# S11494) for 20 min at RT and imaged using a BIO-Rad Gel Docs™ XR+ and quantified using densitometry software ...
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bioRxiv - Developmental Biology 2022Quote: ... 5mL of MEM Non-essential amino acids (100X) ((Thermo Fisher Scientific, Canada. Catalog# 11140050). Day 1 of differentiation cells are first washed with XFM-media to remove any residual E8 media ...
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bioRxiv - Developmental Biology 2022Quote: ... Total ribonucleic acid (RNA) from 250,000 cells was reverse transcribed by Superscript II (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Gels were stained with SYBR Gold nucleic acid gel stain (Thermo Fisher Scientific, USA) and immediately imaged and transferred to positively charged nylon membranes (Roche ...
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bioRxiv - Biophysics 2019Quote: ... acetonitrile and formic acid (99.5 %) Optima™ LC/MS Grade were from Fisher Scientific. Five peptides were used to prepare the peptide mixture ...
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bioRxiv - Immunology 2020Quote: ... 2% MEM non-essential amino acids (MEM NEAA, Gibco, Paisley, UK, reference 11140-035), 50ug/ml gentamycin (Gibco ...
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bioRxiv - Zoology 2020Quote: ... we first used control nucleic acids molecules (BLOCKiT™ Fluorescent oligo control (Invitrogen™), pmaxGFP™ Vector (expressed under a CMV-promoter ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... RWM animals were first incubated in SYTO 60 red fluorescent nucleic acid stain (Invitrogen) diluted to 10 µM in HM for 1 h at room temperature in the dark ...
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bioRxiv - Plant Biology 2019Quote: ... in hydrochloric acid 0.01 N (1:3, v:v) (Fisher Scientific; Hampton, New Hampshire, USA).
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bioRxiv - Plant Biology 2019Quote: ... dissolved in 500 mL of concentrated sulfuric acid (Fisher Scientific; Hampton, New Hampshire, USA) added to 200 mL of MilliQ water (Merck Millipore ...
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bioRxiv - Bioengineering 2019Quote: ... Agarose gels are stained with SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen™) and PFGE patterns are scanned using the Vilbert Lourmat™ E-BOX™ VX2 Complete Imaging system.
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bioRxiv - Biochemistry 2021Quote: ... with 0.1% formic acid and Solvent B was LC-MS grade acetonitrile (Fisher Scientific) with 0.1% formic acid ...
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bioRxiv - Bioengineering 2021Quote: FBS free culture media with 15mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (Gibco) in DMEM/F12 supplemented with 1% P/S was used for all experiments performed in the lung on a chip devices ...
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bioRxiv - Biochemistry 2020Quote: ... anilinonaphthalene-8-sulfonic acid (ANS) were purchased from Fisher Scientific (Fair Lawn, NJ, U.S.A.). Hydrogen Peroxide (30% ...