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Citations for Thermo Fisher :
851 - 900 of 5337 citations for TNF alpha TNFSF2 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... Samples were rinsed and re-suspended in flow buffer containing 25 μL mL−1 of each of the following monoclonal anti-human antibodies per sample: anti-human CD3-AF700 (56-0037-42, ThermoFisher), - CD40L/CD154-FITC (11-1548-42 ...
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bioRxiv - Immunology 2021Quote: ... and total T cells were purified from PBMC suspensions by negative immunomagnetic selection (purity >90%) using the Human Myeloid DC Enrichment Kit (STEMCELL) and the Untouched total human T cell (Invitrogen) kits ...
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bioRxiv - Cancer Biology 2022Quote: ... primary human breast CAFs were transfected with 75 nm human periostin-targeting stealth siRNA (si-POSTN) (ThermoFisher siRNA ID: HSS116400) or 75 nm non-targeting control siRNA (si-Control ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™ ...
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bioRxiv - Microbiology 2022Quote: ... Surface PCDH1 was stained using human anti-EC7 mAb-3677 (5 µg/mL) followed by anti-human Alexa FluorTM 555 (ThermoFisher) for 1 h at 4°C each ...
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bioRxiv - Microbiology 2022Quote: ... encoding full-length human MCT1 (uniprot ID: P53985) and human basigin (uniprot ID: P35613-2) were cloned individually into pFastBac vector (Invitrogen) for recombinant expression in Sf9 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... human ETAR (residues 21-406) and human ETBR (residues 27-424) DNA was cloned into a modified pFastBac vector (Invitrogen), which contains an N-terminal FLAG tag (DYKDDDD ...
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bioRxiv - Neuroscience 2023Quote: ... The human FUS or human TARDBP cDNA was subcloned into the pENTR™/D-TOPO® vector (Thermo Fisher Scientific). To generate the Gateway destination vector pUAST-DEST ...
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bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
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bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
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bioRxiv - Neuroscience 2023Quote: ... and secreted signaling proteins were measured in culture supernatants by the Human Immune Monitoring Center (HIMC) at Stanford University using a Human 48-plex Luminex Procarta Immuno-assay (ThermoFisher).
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bioRxiv - Neuroscience 2023Quote: Human colorectal adenocarcinoma (ATCC, #SK809) and human pancreatic carcinoma (ATCC, #CRL1469) cells were grown in McCoy’s 5A medium (Gibco, #16600082) and Dulbecco’s Modified Eagle’s medium (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... Western blotting was used to confirm reconstituted-protein expression and knockdown efficiency of the generated cell lines using anti-alpha-adaptin (Thermo Fisher Scientific, #AC1-M11) and anti-CALM (Abcam ...
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bioRxiv - Developmental Biology 2020Quote: ... Tgfβ-3(Cat# Mm00436960) alpha-SMA (Cat# Mm00725412) and Vegf (Cat# Mm00437306_m1) Single Tube TaqMan® Gene Expression Assays (Life Technologies Thermo Fisher Scientific) were used ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed with phosphate-buffered saline (PBS) and cultured in growth medium comprising Alpha-Minimum Essential Medium (A-MEM; Invitrogen, Grand Island, NY, USA) supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor 488 at 1:500 (A21121) and alpha-bungarotoxin (BTX)- conjugated to Alexa Fluor 594 at 1:1000 (B13423) from Molecular Probes (Eugene, OR, USA). Tissues were mounted on microscope slides using Millipore mounting fluid (Burlington ...
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bioRxiv - Bioengineering 2020Quote: ... (primary Rabbit anti-mouse alpha-SMA antibody; Cat. No. ab5694; Abcam; 1:50 dilution; secondary Goat anti-rabbit antibody; Cat. No. A11008; Life Technologies; 1:150 dilution). Fluorescent images were acquired using a Leica SP8 confocal microscope ...
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bioRxiv - Bioengineering 2019Quote: ... conditioned media collected from cells was assessed with enzyme-linked immunosorbent assays (ELISA) in order to quantify the concentration of extracellular tumor necrosis factor alpha (TNFα, Invitrogen, cat. #88-7346-88) or Dex (Bioo Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were cultured in a humidified atmosphere of 5% CO2 and 95% air at 37°C in an alpha modification of Eagle’s medium (Invitrogen Life Technologies, Carlsbad, CA, USA) supplemented with 10% FBS ...
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bioRxiv - Genomics 2021Quote: ... The membranes were incubated overnight with UCP1 antibody and loading control protein alpha tubulin antibody for the total protein adipose tissue samples (1:1.000 UCP1 Polyclonal Antibody, cat no. PA1-24894, 1:500 alpha Tubulin Polyclonal Antibody, cat no. PA5-16891, Invitrogen, Thermo Fisher Scientific, Rockford, USA). Membranes with liver and muscle mitochondrial protein samples were incubated overnight with COX4 antibody (1:1.000 COX4 Polyclonal Antibody ...
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bioRxiv - Physiology 2021Quote: ... transferred and blotted with Peroxisome proliferator-activated receptor alpha (PPARα, ab 215270), endothelial nitric oxide synthase (eNOS, sc-376751) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH, Thermo Fisher, #10941-1-AP) antibodies as described [65] ...
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bioRxiv - Cell Biology 2022Quote: ... or embryonic stem cell-derived fibroblasts as in (40, 41) were cultured in complete medium consisting of Minimum Essential Media alpha (MEMα) with GlutaMa™ (Gibco, catalog no. 32561) and 1% Penicillin/Streptomycin (P/S ...
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bioRxiv - Cell Biology 2022Quote: The mouse hepatocyte cell line alpha mouse liver 12 (AML12; CRL-2254, ATCC, Manassas, VA) were cultured in DMEM/F12 (11320033, Thermo Fisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (16140071 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Bone marrow was flushed out of femur and tibia and cultured over-night in alpha-minimum essential medium (α-MEM, Gibco; cat#41061-029)containing 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... ATTO 550+ cells were sorted by flow cytometry and the 3% most positive cells were seeded at 1 cell per well in alpha minimal essential medium (αMEM, Thermo Fisher Scientific, Waltham, MA) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed again and then incubated with SuperSignal West Pico substrate (HA and alpha actinin-1 blots) (Thermo Fisher Scientific, Waltham, MA, #34580) or Supersignal West Femto substrate (FLAG blots ...
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bioRxiv - Biophysics 2023Quote: ... Total tubulin was labeled using a primary antibody against alpha-tubulin and a Cy3-tagged secondary antibody (Thermo Fisher Scientific Inc., Waltham, MA, USA).
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bioRxiv - Microbiology 2020Quote: ... and the Human embryonic kidney (HEK293) cells (Gibco) in Freestyle F17 expression medium (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit anti-human ER(#RM9101,ThermoFisher Scientific, MA). Histological stained slides were scanned using the Aperio ScanScope XT digital slide scanner and positivity analysis for each target gene staining was carried out using Aperio ImageScope V 10.2.1.2314 software ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/ml human basic FGF (Invitrogen, USA), 10 ng/ml recombinant human GDNF (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/ml recombinant human GDNF (Invitrogen, USA) and 1% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human GATA3-PE (clone TWAJ; Invitrogen). BAL cells were collected as described above ...
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bioRxiv - Genomics 2021Quote: ... 20 ng/mL human recombinant EGF (Thermo Fisher), 10 ng/mL cholera toxin ...
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bioRxiv - Microbiology 2021Quote: Human embryonic kidney cells (HEK293FT; Thermo Fisher Scientific), Human hepatocellular carcinoma cells (Huh-7 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or human IFNγ [1000 U/mL (ThermoFisher, USA)] diluted in DMEM and incubated for 24 hours ...
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bioRxiv - Genomics 2020Quote: ... and Human Brain Total RNA brRNA (ThermoFisher AM7962)) ...
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bioRxiv - Immunology 2022Quote: ... 50 ng/mL recombinant human EGF (Gibco, PHG0314), 80 ng/mL recombinant human R- spondin 1 (R & D systems ...
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bioRxiv - Immunology 2022Quote: ... medium supplemented with 2% human AB serum (Gibco). The concentration of IL-2 was increased to 1000 IU/ml on day 7 and the expanded T cells were electroporated with the indicated mRNA at a concentration of 2 pg mRNA/cell on day 8 using 4D Nucleofector™ System (Lonza ...
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bioRxiv - Developmental Biology 2019Quote: ... BMP2 recombinant human protein (Thermo Fisher Scientific, #PHC7145) was applied for 1h on cells ...
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bioRxiv - Neuroscience 2019Quote: Purified (human) fibrinogen with Alexa 488 (ThermoFisher: F13191) was prepared to a final concentration of 2 mg⋅mL−1 ...
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bioRxiv - Genomics 2019Quote: Human brain total RNA was purchased from ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... anti-rat 568 and anti-Human 647 (Invitrogen) were diluted 1:1000 in PBS-Triton X-100 0.1% with 10% FBS ...
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bioRxiv - Microbiology 2019Quote: Human foreskin fibroblasts were maintained in DMEM (Gibco) supplemented with 3% inactivated fetal serum (IFS ...
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bioRxiv - Bioengineering 2019Quote: ... 10 ng/ml human bFGF (Invitrogen Life Technologies), 20 ng/ml human EGF (Life Technologies) ...
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bioRxiv - Bioengineering 2019Quote: ... 10 ng/ml human bFGF (Invitrogen Life Technologies), 20 ng/ml human EGF (Life Technologies) ...
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bioRxiv - Immunology 2019Quote: Human IgG total Ready-SET-Go (Affymetrix ebioscience) was used to measure total IgG following the manufacturer’s instructions (35) ...
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bioRxiv - Immunology 2021Quote: ... and IFN-γ Human Uncoated ELISA Kit (Invitrogen), respectively ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 1% human keratinocyte growth supplement (Gibco), 0.2% GibcoAmphotericin B (250 µg/mL of Amphotericin B and 205 µg/mL sodium deoxycholate ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary human aortic smooth muscle cells (Thermo Scientific) and teloHAEC human immortalized aortic endothelial cells (ATCC ...
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bioRxiv - Biochemistry 2021Quote: ... goat anti-human IgG-HRP (Thermo Fisher, SG) was added at 100 μL/well for an incubation of 60 min in dark ...