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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for Recombinant Human ITGAL & ITGB2 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Hu5F9 and TJC4 were then purified using recombinant Protein A-Sepharose 4B (Thermo Fisher Scientific) buffer exchanged into PBS and concentrated using Amicon Centrifugal Filters (Millipore Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Cell Biology 2023Quote: Recombinant ectodomain proteins were generated by transfection of HEK293T cell cultures using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... then recombinant protein purified from the soluble fraction using HisPur Ni-NTA columns (Thermo Scientific). Purified proteins were dialyzed against EMSA buffer ...
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bioRxiv - Immunology 2023Quote: ... ELISAs were conducted with recombinant full proteins coated overnight in Nunc MaxiSorp 384 plates (ThermoFisher) (2µg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... recombinant proteins were generated using the Bac-to-Bac Baculovirus Expression Systems (Thermo Fisher Sc.). These recombinant proteins were then immobilized on anti-HA magnetic beads and subsequently incubated ...
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bioRxiv - Microbiology 2021Quote: ... 10% HI FBS (Gibco), 1X ultraglutamine (Lonza) ...
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bioRxiv - Microbiology 2021Quote: ... His (ThermoFisher, MA1-21315), FLAG (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... HI FBS (2273356P, Gibco). HEPES (54457 ...
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bioRxiv - Immunology 2020Quote: ... or anti-His (Invitrogen) for recombinant ZVrecE80 antigen in 0.1M carbonate buffer pH 9.6 ...
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bioRxiv - Immunology 2020Quote: ... + 10% HI-FCS (Gibco) + 1% Peniciliin/Streptomycin (100x ...
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bioRxiv - Microbiology 2022Quote: ... anti-penta HIS (Invitrogen) and anti-HSP70 (26 ...
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bioRxiv - Cancer Biology 2023Quote: ... and His-tag (Invitrogen) were used ...
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bioRxiv - Bioengineering 2024Quote: ... 10% HI-FBS (Gibco), 100 U ml−1 penicillin/100 μg ml−1 streptomycin (Gibco) ...
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bioRxiv - Molecular Biology 2024Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Molecular Biology 2024Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Molecular Biology 2024Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Immunology 2021Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermo Fisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS.51 S1-S2-His (referred as S ...
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bioRxiv - Biochemistry 2022Quote: ... The total protein level was detected by primary anti-His-tag antibody (1:10000, MA1-21315, Invitrogen) and secondary anti-mouse antibodies (1:5000 ...
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bioRxiv - Immunology 2022Quote: For purification of the fusion proteins by His Pur™ Ni-NTA Resin (Thermo Scientific, Beijing, USA), the 250 mL IPTG-induced bacterial solution was centrifuged at 4 °C at 12000 rpm for 10 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... Eluted proteins were then incubated with Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific, Waltham, Massachusetts) for 20 minutes and then washed 5 x 1 minute in His-tag Isolation Buffer (100 mM Na-phosphate ...
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bioRxiv - Biochemistry 2023Quote: ... and proteins were visualized using Ponceau stain and Western blot with monoclonal anti-His (Invitrogen MA1-21315), and polyclonal anti-GST (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... we transfected one 100 mm plate of DLD1 BRCA2-/- cells at 70% of confluence with 10 µg of a plasmid containing human EGFP-MBP-tagged BRCA2 cDNA (corresponding to accession number NM_000059) using TurboFect (Thermo Fisher Scientific), 48h post-transfection the cells were serial diluted and cultured in media containing 1 mg/ml G418 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... N-terminal FLAG-tagged human MCC (P23508-1) constructs (pCMV2B vector) were transfected into HEK293 cells and selected with G418 (Gibco #10131035). G418-resistant cells were grown ...
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bioRxiv - Molecular Biology 2020Quote: ... StrepII- and MBP-tagged human PUM1 and PUM2 RD and RBD constructs were expressed in E.coli BL21 (DE3) Star cells (Thermo Fisher Scientific). Cells were grown in LB overnight at 37°C ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells were transfected with plasmid encoding Myc-tagged human NLRP3 using the Lipofectamine™ 3000 transfection kit (L3000015, Thermo fisher). At 24 h after transfection ...
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bioRxiv - Biophysics 2022Quote: ... FLAG-tagged human DNA2 nuclease-deficient (D277A) and helicase-deficient (K654R) mutants were generated by Phusion site-directed mutagenesis (Thermo Fisher) of wild-type DNA2-FLAG (pIF494 ...
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bioRxiv - Cell Biology 2023Quote: Methods used for these assays have been described.28 Briefly, human FLAG-tagged CDKL5 WT or kinase dead (KD, CDKL5 K42R) constructs were subcloned into pT7CFE1-CHis plasmid (Thermo Fisher). These constructs were then used for in vitro translation using a HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-tagged WT or kinase dead (KD, CDKL5 K42R) constructs of human CDKL5 were subcloned into a pT7CFE1-CHis plasmid (Thermo Fisher). A HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with Bovine Pituitary Extract (25 mg) and EGF Human Recombinant (2.5 μg) (Gibco, Life Technologies Limited, USA). This medium was established through previous successes in obtaining cell lines from primary tissue [26] ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... supplemented with human recombinant epidermal growth factor (rEGF) and bovine pituitary extract (BPE) plus 2% penicillin-streptomycin (Gibco) at 37°C in 5% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... and 1 μg/ml Recombinant Human Fibroblast growth Factor-basic (FGFb) (Catalog # 13256-029 Gibco, Thermo Fisher Scientific) at 37°C with 5% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... and 1 μg/ml Recombinant Human Fibroblast growth Factor-basic (FGFb) (Catalog # 13256-029 Gibco, Thermo Fisher Scientific) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... EGF-Cy3B was generated using NHS-Cy3B (Cytiva, Marlborough, MA) conjugated to recombinant human EGF (Gibco, ThermoFisher Scientific), using 1:1 labeling stoichiometry as described previously 60 ...
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bioRxiv - Cell Biology 2022Quote: ... EGF-Cy3B was generated using NHS-Cy3B (Cytiva, Marlborough, MA) conjugated to recombinant human EGF (Gibco, ThermoFisher Scientific), using 1:1 labeling stoichiometry as described previously 60 ...
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bioRxiv - Cell Biology 2022Quote: ... at 37 °C and 5% CO2 in EMEM (ATCC) supplemented with 0.01 mg/mL human recombinant insulin (Gibco) and 10% (vol/vol ...
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bioRxiv - Immunology 2020Quote: ... were used for T cell activation and expansion in the condition of human recombinant IL-2 (Gibco, PHC0021) or where indicated ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% penicillin–streptomycin in 6-well plates coated with truncated recombinant human vitronectin (Thermo Fisher Scientific). The iPSC line was previously assessed for pluripotency and normal karyotype.33 For differentiation ...
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bioRxiv - Neuroscience 2022Quote: ... hiPS cells were maintained in 6-well plates coated with recombinant human vitronectin (VTN-N, Life Technologies, A14700) in Essential 8 medium (Life technologies ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs were maintained in six-well plates coated with recombinant human vitronectin (VTN-N, Thermo Fisher Scientific, A14700) in Essential 8 medium (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: Cell culture medium Essential 8 (E8) Flex Medium and recombinant human vitronectin (rhVTN-N) were purchased from Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 0.055 mM β-mercaptoethanol and 10 ng/ml recombinant human full-length bFGF (All reagents from Gibco, USA)].
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured in six-well plates coated with recombinant human vitronectin (Life Technologies, catalogue no. A14700) using Essential 8 medium (Life Technologies ...