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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for Recombinant Human GPD1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... AcMNPV recombinant bacmids were constructed with the bMON14272 bacmid (Invitrogen) and maintained in Escherichia coli strain DH10B (Invitrogen) ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 μL of RNaseOut™ Recombinant RNase Inhibitor (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant SRPK1 and PKR were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant clones were selected with 120 μg/ml Hygromycin (Invitrogen) and pooled to obtain a stable population maintained in medium containing Puromycin ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... 200 U/mL RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher, 10777019), 400U/mL RNasin (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10 ng/mL recombinant bFGF (Thermo Fisher Scientific, PHG0261). Human iPSCs were converted to EPS cells referred to protocol described previously16 ...
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bioRxiv - Cell Biology 2021Quote: Recombinant constructs were expressed in E.coli BL21 DE3 cells (Invitrogen) in Luria Bertani media ...
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bioRxiv - Biophysics 2020Quote: The recombinant tubulin was expressed in HighFive cells (Life Technologies) and purified by three steps of column chromatography (DEAE Sepharose ion exchange chromatography ...
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bioRxiv - Immunology 2021Quote: ... cells were pretreated with mouse recombinant IFN-γ (BMS326, Invitrogen) at 6.25ng/mL for overnight and followed by infection with Mycobacterium tuberculosis ...
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bioRxiv - Immunology 2021Quote: ... cells were pretreated with recombinant IL-13 (PMC0134, Thermo Scientific) at 20ng/mL and IL-4 (PMC0045 ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with recombinant murine EGF (100 ng ml−1, Gibco), recombinant human R-spondin 1 (200 ng ml−1 ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 ng/ml recombinant mouse EGF (Thermo Fisher Scientific, PMG8041), 1 ug/ml recombinant human R-spondin1 (R&D ...
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bioRxiv - Neuroscience 2022Quote: ... AlexaFluor™ 555 cholera toxin subunit B (recombinant) (Invitrogen #C22843)] of 1mg/mL (Molecular Probes® ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were transfected with recombinant bacmids using Cellfectin II reagent (Gibco-Thermo Fisher Scientific™ ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 50 ng/ml mouse recombinant EGF (Invitrogen, PMG8043). The organoids were trypsinized with 0.05% Trypsin-EDTA (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with recombinant murine EGF (100 ng mL-1, Gibco), recombinant human R-spondin 1 (200 ng mL-1 ...
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bioRxiv - Genomics 2023Quote: ... 200 units/mL RNaseOUT™ Recombinant Ribonuclease Inhibitor (#10777019; ThermoFisher), and 1X cOmplete ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.15 μL recombinant RNase Inhibitor (40 U/μL, Thermo Fisher); 0.06 μL Dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2023Quote: ... 80 U of RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher), and 25 μl of T7 RNA polymerase (lab made) ...
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bioRxiv - Genomics 2023Quote: ... 0.5 μl of RNaseOUT Recombinant Ribonuclease Inhibitor (ThermoFisher, 10777-019) to the hexamer annealed RNA ...
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bioRxiv - Biophysics 2023Quote: ... Tetravalent recombinant (wild-type) streptavidin was procured from Thermo Scientific. Plasmids encoding SBD2 (T369C/S451C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10 ng/mL recombinant mouse interleukin-3 (Gibco PMC0031).
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bioRxiv - Cell Biology 2024Quote: ... 200U/mL RNase OUT Recombinant Ribonuclease Inhibitor (Invitrogen, 10777–019), pH 8 ...
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bioRxiv - Cell Biology 2024Quote: ... In summary: the Taq DNA Polymerase Recombinant 500U (Invitrogen™) was used to perform all the PCRs ...
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bioRxiv - Biochemistry 2024Quote: ... all recombinant plasmids were constructed using Gateway technology (Invitrogen, USA). Genes of interest (GOI ...
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bioRxiv - Cancer Biology 2024Quote: ... recombinant mouse EGF (Thermo Fisher Scientific, #PMG8045, 40 ng/ml), CHIR99021 (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... and 50 ng/mL recombinant murine epidermal growth factor (Invitrogen). Every 3-4 days ...
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bioRxiv - Neuroscience 2024Quote: ... Phospho-PKA alpha/beta (Thr197) Recombinant Polyclonal Antibody (Invitrogen, 711615) and PKA alpha Polyclonal Antibody (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Human T cells were activated with human CD3/CD28 Dynabeads (Invitrogen), at a bead:cell ratio of 2:1 and transduced using supernatant collected from Phoenix-AMPHO cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used for human samples were anti-human CD31 (#17031942, Invitrogen), anti-human CD45 (#17945942 ...
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bioRxiv - Genomics 2020Quote: ... SEMA3C protein levels were checked by western blot as described above using rabbit anti-human SEMA3C antibody (1:1000, Thermo Fisher Scientific). Beta-actin (housekeeping gene ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Colorimetric protein assays were conducted using commercial human IL-8 ELISA and mouse IL-6 ELISA kits (Invitrogen; 88-8086, 88-7064) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Raw MS Data were searched against the human SwissProt protein database (Version November 2020) with Proteome Discoverer 2.4 (ThermoFisher Scientific, Bremen, Germany) using the following parameters ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells in 35-mm dishes were transfected with 100 pmol of each siRNA in the Stealth siRNA library targeting 154 human nuclear proteins (Invitrogen–Thermo Fisher Scientific) using Lipofectamine RNAiMax (Invitrogen–Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... operated by UNICORN software version 5.11 (Build 407) using HiTrap Protein A columns (Cytiva) for full length human mAbs and CaptureSelect CH1-XL MiniChrom columns (Thermo Fisher Scientific) for Fab fragments ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10428) and 1-Step Human Coupled IVT Kit – DNA (#88882) were obtained from Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2022Quote: ... All S constructs were verified by Sanger sequencing and the protein was subsequently produced in human embryonic kidney (HEK) 293F cells (Thermo Fisher Scientific) and purified as previously described (4 ...
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bioRxiv - Neuroscience 2022Quote: ... similarly as described previously.[60] MS data were analyzed using an in-house maintained human protein sequence databases using SEQUESTTM and Proteome DiscovererTM (Thermo Fisher Scientific). The mass tolerances of a fragment ion and a parent ion were set as 0.5 Da and 10 ppm ...
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bioRxiv - Immunology 2024Quote: ... tagged with human IgG Fc was transiently transfected using FreeStyle 293-F cells and purified using Protein G Plus Agarose (Pierce Thermo Fisher Scientific). RBD-SD1 was cleaved from the Fc by incubation with 3C protease (produced in-house ...
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bioRxiv - Immunology 2023Quote: Plasmids coding for the soluble S ectodomain proteins as well as the RBD of human coronaviruses were transfected into Freestyle293F cells (Thermo Fisher R79007). Per 1 L of Freestyle 293F cells ...
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bioRxiv - Neuroscience 2023Quote: ... Human brain tissue was homogenised with a Dounce homogeniser and placed in a low protein binding 15mL tube (Thermo Fisher Scientific, 30122216) containing 10mL 1X artificial CSF (pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: Plasmids encoding human ZFP36 proteins TTP and Brf1: human ZFP36 and ZFP36L1 open reading frames were amplified from the ORFeome cDNA clone library (Invitrogen, Life Technologies) by Phusion PCR (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: MS/MS searches were carried out using SEQUEST search algorithms against a Uniprot database for humans (supplemented with exogenous sequence for anticipated protein constructs) using Proteome Discoverer (Version 3.0, Thermofisher Scientific, Bremen, Germany). Oxidation of methionine and N-terminal protein acetylation were included as dynamic modifications and carbamidomethylation of cysteine was set as a static modification ...
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bioRxiv - Cancer Biology 2021Quote: ... human BNIP3 (Hs00969291_m1) and human 18S (Hs99999901_s1) were purchased from Applied Biosystems. 18S served as an internal control ...
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bioRxiv - Microbiology 2022Quote: Human monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (83) ...
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bioRxiv - Cell Biology 2020Quote: ... Ki-67 recombinant rabbit monoclonal antibody (SP6) (Thermofisher, Cat. #: MA5-14520), Goat anti-Mouse IgG (H+L ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant soluble ACE2 was coated on NUNC Maxisorp plates (Thermo Scientific) at 1µg/well at RT for 3 h ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant gene was cloned into pFastBac1 expression vector (Life Technologies) for bacmid production ...
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bioRxiv - Cell Biology 2019Quote: Recombinant Bax was labeled by NBD dye (IANBD amide, Life Technologies) as reported previously (Kale et al. ...