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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for OF 1 CAS 919973 83 4 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... and 4’,6-diamidino-2-phenylindole (DAPI, Life Technologies, 1:250) to reveal actin and the nucleus ...
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bioRxiv - Bioengineering 2020Quote: dLNs were fixed for 1 hour with 4% paraformaldehyde (Thermofisher Scientific), embedded in Tissue-tek OCT (VWR ...
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bioRxiv - Neuroscience 2021Quote: ... GPC-4 (#13048-1-AP, Thermo Scientific, and #MABN528, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... overnight at 4 °C and alpha-Tubulin (Invitrogen, 11224-1-AP) for 1-2 hours at room temperature ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Aliquots of 4 µL of 1 mM Mitotracker Green FM (ThermoFisher) stock solution were added to the samples and were kept in the dark at 15°C for 15–20 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 1:200 rabbit anti-FABP-4 (Thermo Fisher Scientific, PA5-30591), 1:200 rabbit anti-Osteocalcin (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... and subsequently 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, 1:50000) for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... the aliquots were incubated with 4-pentyn-1-o1 (ThermoFisher Scientific) and ADPRC (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... KP-4 and PANC-1 cells were maintained in DMEM (Gibco). LOX-IMVI cells were maintained in RPMI 1640 medium (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... 4’,6-diamidino-2-phenylindole (DAPI, 1:50000 dilution, Molecular Probes) was treated in the cells for 3min ...
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bioRxiv - Cancer Biology 2022Quote: ... ki67 (ThermoFisher, Cat. RM-9106; 1:50 overnight at 4 °C), βIII-tubulin (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... and mouse anti-HuC/D (Elavl3/4) (1:500, Invitrogen A21271) in PBST overnight at 4 °C in a humidified chamber ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 μL of 1 μM Fluo-4 AM (ThermoFisher Scientific, F14021) in GBO medium was added to each GBO at the ALI surface for at least 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 4’,6’-diamino-2-fenil-indol (DAPI) (1:25000, Life Technologies) and Phalloidin (Alexa 488 ...
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bioRxiv - Microbiology 2023Quote: ... plus DAPI (Thermo Fisher Scientific, 1:1000 in 4% BSA PBS) at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... and 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, #D1306, 1/1000) were incubated overnight at 4°C in the dark ...
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bioRxiv - Genomics 2023Quote: ... 1X 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) (Thermo Fisher Scientific), 1X MEM non- essential amino acids (Thermo Fisher Scientific) ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: Astrocytes were loaded with Fluo-4 AM (1 mM; Molecular Probes) in culture media for 20 min at 37°C followed by 10 min wash in HBSS (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... 4% Surecast solution (Acrylamide:Bis-acrylamide ratio of 29:1, ThermoFisher #HC2040) with 0.5% VA-044 activator (Wako ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (Gibco) and 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... at 4°C overnight or 1:500 AlexaFluor-488-streptavidin (Invitrogen) for 48 hours ...
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bioRxiv - Synthetic Biology 2024Quote: ... anti-mouse-IL-4-APC (17-7041-82, Invitrogen, 1:50) or anti-mouse-IL-17-eFluor™ 450 (48-7177-82 ...
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bioRxiv - Neuroscience 2020Quote: ... Fluo-4 (Fluo 4 AM/Fluo 4 direct, Life Technologies) was used according to the manufacturer’s instructions and a previously published protocol (54) ...
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bioRxiv - Neuroscience 2021Quote: ... stained with rabbit polyclonal antibodies against mouse ZO-1 (1:100, Invitrogen, Carlsbad, CA, USA) and visualized with Alexa Fluor 594 (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% non-essential amino acids and 1% penicillin/streptomycin (all from Life Technologies, Carlsbad, CA) under 37 °C and 5% CO2 conditions ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then nutated overnight at 4 °C with 1 ml of Protein G Sepharose 4 Fast Flow slurry (Invitrogen) per 25ml of supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... free-floating coronal hippocampal slices of 50 µm thickness were produced and incubated overnight in staining solution (4% normal goat serum, 0.4% Triton-X 100 and 4% BSA) with anti-GFAP antibody (1:1000, ThermoFisher, rat) and anti-Iba1 antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: Handpicked islets (~180) from control (n=4) and diabetic (n=4) mice were placed into 1 mL Trizol (Cat# 15596018, Ambion) and processed for RNA extraction ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% (KCL-22, K562, REH, NB-4) or 20% (MOLT-4, OCI-AML3, MOLM-13, Kasumi-1) fetal bovine serum (Gibco) and 1% penicillin–streptomycin (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... at physiological pH before being spun down and resuspended in PBS with the addition of FM 1-43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide (Cat. no. T3163, Invitrogen). Images were taken on a Zeiss X10 light microscope and cell area was measured using CellProfiler 4.2.1 (33,34).
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bioRxiv - Molecular Biology 2023Quote: ... stained with N-(3-triethylammonium propyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM 1-43FX, Invitrogen, Cat.-No. F35355) at 5.6 µg ml-1 ...
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bioRxiv - Neuroscience 2024Quote: ... tissues were washed 3x for 1 hr in adult brain wash solution and incubated overnight at 4°C or for 4 hr at room temperature with AlexaFluor488 anti-rat (1:250) (Invitrogen) and DAPI (1:250 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... were labeled in loading buffer (HBSS with 1% FBS, 1 mM CaCl2 and 1 mM MgCl2) containing 1 μM Fluo-4 AM (Thermo Fisher) with 0.02% Pluronic F-127 at 37 °C for 30 min with gentle agitation every 10 min ...
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bioRxiv - Genetics 2021Quote: ... worms grown at 25°C were incubated 48 hours prior to imaging with 2 μM LysoTracker Red DND-99 (Life Technologies). Worms were imaged as day 4 adults as described above ...
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bioRxiv - Cell Biology 2020Quote: ... for 10 minutes in live-cell imaging medium and lysosomes were visualized by staining cells with 50nM Lysotracker Red DND-99 (Thermo Fisher) for 30 minutes in live-cell imaging medium according to manufacturer’s description ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... each well was gently rinsed once with RPMI and then starved for 50 min in 2 mL of RPMI (without amino acids, glucose or FBS) with 20 μM Lysotracker Red DND-99 (Invitrogen, L7528). After 50 min of starvation ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell differentiation was induced when cells reached around 90% confluence by replacing the proliferation medium with 99% (v/v) high glucose DMEM (Gibco, UK), 1% (v/v ...
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bioRxiv - Neuroscience 2024Quote: For the lysosomal functionality assay fibroblast cells were incubated with CM containing 100 nM LysoTracker™ Red DND-99 (Thermo Fisher) for 5h ...
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bioRxiv - Cancer Biology 2024Quote: ... LMD samples were incubated at 99 °C for 30 min followed by 50 °C for 10 min with SMART trypsin (ThermoFisher Scientific). SMART trypsin was added at a ratio of 1 μg per 30 mm2 tissue ...
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bioRxiv - Cell Biology 2022Quote: Lysosomal mass was estimated by incubating cells under various genetic perturbations (7 days after sgRNA transduction) with LysoTracker Red DND-99 at 100 nM (Invitrogen; L7528). After incubation at 37ºC for 1 hour ...
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bioRxiv - Physiology 2023Quote: ... the gut of control or rapamycin-treated flies were dissected out in 4°C chilled PBS and then stained in a 10 μM Lysotracker Red:1μg/ml Hoechst solution (LysoTracker™ Red DND-99, Hoechst 33342 Solution, Thermofisher Scientific) for 3 minutes ...
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bioRxiv - Neuroscience 2024Quote: Single-molecule (sm) FISH was performed using the ViewRNA Cell Plus kit according to manufacturers protocol (Thermo Fisher - 88-19000-99) with probes for RERE mRNA (vc4-3148005-VCP ...
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bioRxiv - Microbiology 2024Quote: ... Intracellular crystal violet was extracted from the adherent cells in 2 mL of 99% ethanol and absorbance was measured at 575 nm in a Genesys 20 spectrophotometer (Thermo Scientific). Wells containing uninoculated 1/4X THYE were included in the experimental design and used as negative controls.
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bioRxiv - Cancer Biology 2021Quote: ... followed by 1 μg/ml DAPI (Thermo Fisher Scientific, CA, USA) staining for 10 minutes at room temperature and Prolong® Gold Antifade Reagent mounting (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 1% Igepal CA-630 and Halt protease inhibitor cocktail (Thermofisher, #78429). After rocking 30 min at 4°C in lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... 1% insulin/transferrin/selenium A (all from Life Technologies, Carlsbad, CA), 0.4 μg/ml hydrocortisone (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2021Quote: ... rabbit anti-goat IgG-HRP (1:3,000 dilution) (Invitrogen, Carlsbad, CA), and goat anti-human IgG-HRP (1:3,000 dilution).
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bioRxiv - Developmental Biology 2021Quote: ... goat Alexa 555 anti-rabbit (1:1000, Invitrogen, Carlsbad, CA, #A21429); or donkey Alexa 647 anti-mouse (1:1000 ...
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bioRxiv - Biophysics 2021Quote: ... either Transforming growth factor beta-1 (TGF-β1, Invitrogen, CA, USA) or epidermal growth factor (EGF ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM Phenylmethylsulfonyl fluoride (PMSF; ACROS Organics, Cas#329-98-6), 5 mM β-ME ...