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Citations for Thermo Fisher :
851 - 900 of 4028 citations for Influenza Virus Hemagglutinin HA H7N9 Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Recombinant NRX1β-Fc and NRX1βΔHRD-Fc proteins were pre-immobilized with Protein G Magnetic Beads (Dynabeads Protein G, Cat# 10004D, Thermo Fisher Scientific) in PBS overnight at 4 °C with constant agitation ...
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bioRxiv - Cell Biology 2023Quote: ... Subsequently another round of blocking with 10% FBS/ PBS at 37°C or at 4°C overnight was followed by a second round of subsequent incubation with αCSP (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and secondary anti-mouse Alexa Fluor 546 antibodies (1:300 diluted in 10% FCS/ PBS; Life technologies) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: Cells in the exo-erythrocytic development assays were subsequently stained with a primary antibody against Plasmodium berghei HSP70 (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and an anti-mouse Alexa Fluor 488 (1:300 diluted in 10% FCS/ PBS; Life technologies). Hoechst 33343 was added after for 5 min at RT and the cells washed twice in 1% FCS/ PBS the assays before being mounted ...
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bioRxiv - Cell Biology 2023Quote: Cells in the invasion assay were stained subsequently for 1 h at 37°C with primary mouse αCSP antibodies (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and anti-mouse Alexa Fluor 488 antibodies (1:300 diluted in 10% FCS/ PBS; Life technologies). Following two washing steps with 1% FCS/PBS the cells were permeabilized by addition of ice-cold methanol for 10 min at RT ...
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bioRxiv - Developmental Biology 2020Quote: ... The next day PcDNA 3.1/ Twist1-BirA*-HA plasmid or PcDNA 3.1/ GFP-BirA*-HA plasmid was transfected into cells using Lipofectamine® 3000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with 7μl Mouse monoclonal anti-HA.11 (Ascites Covance MMS-101R) and 20μl Rabbit polyclonal anti-HA (Invitrogen cat#71-5500) at 4°C while rotating ...
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bioRxiv - Cell Biology 2021Quote: ... pInd20-Maf1-HA was cloned by taking Maf1-HA from pFTREW-Maf1-HA (16) into a pInducer20 construct by gateway cloning using LR clonase (Thermo Fisher). The empty pInducer20 vector was a gift from Stephen Elledge (Addgene #44012 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were transfected with 30 μg pcDNA3-Htt-ex1-Q25-HA or pcDNA3-Htt-ex1-Q97-HA using Lipofectamine 2000 (Thermo Fisher), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: SEC-purified HA-VLPs and HA-NPs were prepared on grids for cryo-ET using a Mark IV Vitrobot (ThermoFisher Scientific) operated at 21°C and 100% humidity ...
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bioRxiv - Cell Biology 2023Quote: ... The axonemal proteins were incubated overnight with anti-HA magnetic beads with bound anti-HA antibodies (Thermo Fisher Scientific, Waltham, MA) at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 2x 15cm dishes of cells per SILAC label per replicate were used to perform HA-immunoprecipitation using 60 µl slurry of Anti-HA antibody coated magnetic beads (ThermoFisher, 13474229). Cells were lysed in normal lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: HeLa cells were transfected with HA-RPS27A-Myc or HA-RPS27AP5-Myc constructs using Lipofectamine LTX reagent (Thermo Fisher Scientific, USA), and were incubated for 48 h ...
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bioRxiv - Microbiology 2024Quote: ... Western blotting and immunodetection of HA-tagged proteins with anti-HA and horse radish peroxidase-coupled secondary antibodies using an iBright™ FL1500 (ThermoFisher). The remaining 25 µl bead suspension was used as input for on-bead digestion (Klaus et al. ...
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bioRxiv - Microbiology 2021Quote: ... Spike protein (C9-tag) on the surface of pseudovirus was detected using a mouse anti-C9-tag monoclonal antibody 1D4 (Invitrogen, Cat. No. MA1-722), and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... Avi-tag for biotinylation and 6xHIS tag for Nickel purification using the HisPur™ Ni-NTA Spin Purification Kit (Thermo Fisher Scientific, Waltham, MA). The coated plates were washed 3x 300μl PBST and blocked for 1 hour in 1% BSA/PBS before adding a 1/3 titration of mAbs in assay diluent (0.5% BSA/PBS/0.05% Tween-20 ...
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bioRxiv - Microbiology 2020Quote: ... N-term B virus mut) using a Neon® Transfection System (Invitrogen) under the recommended electroporation condition (1350 V ...
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bioRxiv - Molecular Biology 2021Quote: ... We used TaqMan® Fast Virus 1-Step Master Mix (ThermoFisher Scientific) to set up 10 μl (half volume ...
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bioRxiv - Biochemistry 2019Quote: ... Recombinant virus was made by co-transfection into SF9 insect cells (Invitrogen) of the plasmid and BacVector3000 baculovirus DNA (Novagen ...
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bioRxiv - Systems Biology 2019Quote: ... and MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies), and the reaction was carried according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... and MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies), and the reaction was carried according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The virus samples were treated with 300 units of RNase I (Ambion) for 30 min followed by SUPERaseI RNase Inhibitor (Thermo Fisher ...
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bioRxiv - Cancer Biology 2019Quote: ... 2.5mL virus with 10μg/mL polybrene (Thermo Fisher Scientific TR-1003-G) was added to 5×105 cells plated in 6-well dishes ...
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bioRxiv - Microbiology 2020Quote: ... The virus was rescued and propagated in T-REx-293 cells (Invitrogen). Purification was by CsCl gradient ultracentrifugation ...
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bioRxiv - Cancer Biology 2020Quote: ... Concentrated virus was filtered using 0.45um syringe filters (ThermoFisher; Cat#: 725-2545) and immediately added to cultured OSE in 24-well plates ...
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bioRxiv - Microbiology 2020Quote: ... Stocks of virus were grown in Vero-E6 cells in DMEM (Gibco) supplemented with 10% fetal bovine serum (Atlas Biologicals ...
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bioRxiv - Microbiology 2020Quote: ... containing 7,5 μl 4xTaqMan Fast Virus 1-Step Master Mix (Life Technologies) and 1 μl Primer/Probe mixture29,30 ...
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bioRxiv - Microbiology 2020Quote: ... the virus particles were resuspended in CO2-independent media (ThermoFisher, Cat# 18045088), aliquoted ...
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bioRxiv - Immunology 2019Quote: ... purified virus was immobilized on 96-well maxisorp microtiter plates overnight (Nunc). Wells were blocked with 0.05% PBST [0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... c-MYC and KLF4 in CytoTune Sendai virus vectors (Thermo Fisher Scientific) (Sontag et al. ...
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bioRxiv - Microbiology 2021Quote: ... The virus was rescued and propagated in T-REx-293 cells (Invitrogen). Purification was by CsCl gradient ultracentrifugation ...
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bioRxiv - Immunology 2021Quote: ... Vero cells were used to propagate the virus in DMEM medium (Gibco) supplemented with 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Virus was removed from cells and replaced with post-infection media (Gibco OptiMem supplemented with 0.01% FBS ...
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bioRxiv - Microbiology 2022Quote: ... the reactions with Taqman Fast Virus 1-step master mix (ThermoFisher scientific) were half of the recommended 20 μl volume ...
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bioRxiv - Neuroscience 2023Quote: ... The virus-containing pellet was resuspended in 200 μL of DPBS (ThermoFisher) and stored at -80 °C ...
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bioRxiv - Microbiology 2023Quote: ... 10-fold dilutions of each virus in DMEM + 1% antibiotic/antimycotic (Gibco) were added to a monolayer Vero E6-TMPRSS2-T2A-ACE2 cells for 1 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Each virus was mixed with PBS-washed defibrinated sheep blood (Fisher Scientific) containing 5 mM ATP to a concentration of 106 PFU/mL and fed to ∼50-60 female mosquitoes ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis was performed with Moloney murine leukemia virus reverse transcriptase (Invitrogen) at 38°C for 60 min ...
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bioRxiv - Cell Biology 2020Quote: ... and/or V5 tags were constructed with pcDNA6 vector (Invitrogen). Recombinant human Lsm12-expression plasmids were obtained either commercially (pCMV-Lsm12-Myc-FLAG from OriGene ...
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bioRxiv - Genetics 2021Quote: ... triacylglycerol or TAG (Pointe Scientific Triglycerides Liquid Reagents; Fisher Scientific); non-esterified fatty acids or NEFAs (HR series NEFA-HR[2] Reagents ...
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bioRxiv - Immunology 2021Quote: ... anti-VSVg tag-158 Gd (rabbit pAb, Thermo Fisher Scientific), anti-E tag-154Sm (clone 10B11 ...
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bioRxiv - Neuroscience 2021Quote: ... and labelled with TMT10plex Mass Tag Labelling kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... labeled with 16-plex tandem mass tag reagents (Thermo Scientific) in 100 mM triethylammonium bicarbonate ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with primary antibodies against V5 tag (R960- 25, ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His tag Antibody (MA1-125 21315) was from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-DYKDDDDK epitope (FLAG) tag (Thermo Scientific Pierce, MA1-91878), Anti-HA (Thermo Scientific Pierce ...
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bioRxiv - Neuroscience 2020Quote: ... coli expression vector pDEST15 with GST tag Gateway Vector (Invitrogen). MYC-LRRK2-WT was a gift from Dr ...
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bioRxiv - Cell Biology 2020Quote: ... anti-S-tag mouse monoclonal antibody (MA1-981; Thermo Fisher), rabbit polyclonal anti mNeonGreen Tag (53061S ...
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bioRxiv - Systems Biology 2020Quote: ... We dissolved tandem mass tag (TMT) 10plex reagents (Thermo Scientific) in anhydrous acetonitrile (0.8 mg/82 μl) ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary C-MYC tag antibody (PA1-981, Thermo Fisher Scientific) and N-MYC Polyclonal antibody (PA5-17403 ...
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bioRxiv - Biochemistry 2022Quote: ... and anti-GST tag monoclonal antibody MA4-004 (ThermoFisher Scientific). The primary antibodies were detected with secondary anti-rabbit and anti-mouse antibodies (LI-COR Biosciences) ...