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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for Human High Sensitive Interleukin 8 IL8 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) and amplified in triplicate on the QuantStudio 12K Flex system with PowerSYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Genomics 2023Quote: ... cDNA was generated using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Quantitative PCR reactions were performed using PowerSYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... Purified products were quantified with qubit high sensitivity dsDNA kit (Invitrogen, #Q32851) and prepared and submitted according to Azenta life sciences (Genewiz ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were quantified using Qubit dsDNA High Sensitivity assay kit (ThermoFisher Scientific). Fragment sizes of final libraries were assessed and quantified using DNA High Sensitivity Bioanalyzer Chip (Agilent) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... high capacity cDNA kit and power SYBR green were obtained from ThermoFisher Scientific (Loughborough ...
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bioRxiv - Genomics 2023Quote: ... concentration was determined via QbitⓇ dsDNA High Sensitivity Assay Kit (Invitrogen Q32851), and library size and quality was determined via Bioanalyzer (Agilent High Sensitivity DNA Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were quantified using Qubit dsDNA High Sensitivity assay kit (ThermoFisher Scientific). Fragment sizes of final libraries were assessed and quantified using DNA High Sensitivity Bioanalyzer Chip (Agilent) ...
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bioRxiv - Molecular Biology 2023Quote: ... The SMRTbells were quantified with Qubit dsDNA high-sensitivity kit (ThermoFisher Scientific) and qualified by capillary electrophoresis as above ...
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bioRxiv - Cell Biology 2023Quote: ... 85°C for 5 minutes (High Capacity cDNA Reverse Transcription Kit, ThermoFisher). Following conversion ...
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bioRxiv - Cancer Biology 2023Quote: The High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA) was used to create first-strand cDNA from total RNA ...
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bioRxiv - Bioengineering 2023Quote: ... cDNA was generated using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems), according to manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2024Quote: ... The High Capacity cDNA Reverse Transcription Kit (Ref 4368814) from Applied Biosystems was used to produce cDNA following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... amplified by PCR (Phusion High-Fidelity DNA polymerase kit, Thermo Fisher Scientific) and purified by phenol:chloroform extraction ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized by High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and reverse-transcribed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) and random primers ...
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bioRxiv - Molecular Biology 2024Quote: ... using a High-Capacity Reverse Transcription Kit (Applied Biosystems, Foster City, CA). The cDNA was diluted ten times prior to PCR amplification ...
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bioRxiv - Physiology 2024Quote: ... reverse transcribed utilizing High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Lithuania). cDNA served as template for real-time quantitative PCR (RT-qPCR) ...
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bioRxiv - Systems Biology 2024Quote: ... Libraries were quantified by Qubit with the dsDNA High Sensitivity Kit (ThermoFisher) and run on a Fragment Analyzer to confirm high quality of each library prior to sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... Human iPS cells were maintained and propagated as colonies in feeder-free conditions in Essential 8 Flex Medium (Thermo Fisher Scientific, A2858501) on Matrigel hES qualified (Corning ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... maintenance culture was carried out for all cell lines including human ES cells using albumin-free Essential 8 Medium (Thermo Fisher Scientific) in six-well feeder-free culture dishes coated with 5 μg/mL vitronectin (VTN-N ...
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bioRxiv - Microbiology 2020Quote: ... HCT-8 human ileocecal colorectal adenocarcinoma cell line and its 5-FU-resistant was cultured in RPMI 1640 medium (Gibco, New Zealand) containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Evolutionary Biology 2023Quote: The region of the elephant shark genome homologous to human CNE1426 was amplified by PCR and subcloned pCR™8/GW/TOPO® (Invitrogen) (the primer sequences are in Extended Data Table 1) ...
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bioRxiv - Bioengineering 2023Quote: ... at pH 8 containing 8 M urea (Thermo Fisher Scientific, USA). To reduce the sample ...
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bioRxiv - Developmental Biology 2023Quote: Essential 8 medium consisted of essential 8 basal medium (Gibco, A1517001) supplemented with 50x essential 8 supplement.
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bioRxiv - Immunology 2021Quote: ... Amplicons were generated using 100 ng DNA as input in a final volume of 40 μL (consisting of 8 μl 5x Phusion GC buffer (Phusion High-Fidelity DNA Polymerase set (ThermoFisher Scientific, Cat#F534), 0.8 μL dNTPs (dNTP Set ...
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bioRxiv - Molecular Biology 2023Quote: The Aire exons 6 and 8 that were the targeted genomic regions were amplified through conventional PCR using PlatinumTM high fidelity Taq polymerase (Thermo Fisher, Cat # 11304011) and the oligonucleotide primers as mentioned above.
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bioRxiv - Synthetic Biology 2021Quote: ... The T cells were isolated using the EasySep human T cell isolation kit (Stemcell) and activated with human T-activator CD3/CD28 Dynabeads (Gibco) at a bead:cell ratio of 1:1 ...
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bioRxiv - Immunology 2019Quote: ... human PBMC derived T cells were purified by Human T cell isolation kit from Invitrogen (Invitrogen Dynal AS, Oslo, Norway) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... and total T cells were purified from PBMC suspensions by negative immunomagnetic selection (purity >90%) using the Human Myeloid DC Enrichment Kit (STEMCELL) and the Untouched total human T cell (Invitrogen) kits ...
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bioRxiv - Neuroscience 2023Quote: ... A HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA, 88881, Life Technologies) enabled in vitro translation of these constructs ...
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bioRxiv - Biochemistry 2020Quote: ... an in-house generated model of human hepatic Huh-7 cells stably overexpressing mRFP-Rab5 were cultured in DMEM/GlutaMax/High glucose (Gibco) supplemented with 10 % FBS ...
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bioRxiv - Microbiology 2020Quote: ... and human hepatoma Huh7.5 (Apath) cells were maintained in high-glucose Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen, 10-013-CV); African green monkey kidney Vero (ATCC ...
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bioRxiv - Genomics 2024Quote: ... human chronic myelogenous leukemia cancer cell line K562 and human embryonic epithelial kidney cell line HEK293T were cultivated in Dulbecco’s Modified Eagle Medium with high glucose (#11965084, Life Technologies) supplemented with 10% fetal bovine serum Life Technologies ...
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bioRxiv - Developmental Biology 2022Quote: Human HEK293 cells were maintained at 37°C in complete media consisting of DMEM with high glucose GlutaMAX Supplement (Gibco) and 10% Fetal Bovine Serum ...
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bioRxiv - Cell Biology 2023Quote: ... human pluripotent stem cells were dissociated using Accutase and plated at high density on Geltrex (#A14133-02, Thermo Fisher Scientific) coated wells ...
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bioRxiv - Immunology 2023Quote: ... The low and high molecular weight membranes proteins were incubated with respective primary antibodies (Rabbit anti-human BAIAP2L1, PA5-54000, Invitrogen) or (Goat anti-mouse GAPDH ...
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bioRxiv - Microbiology 2024Quote: Immortalized human HaCaT keratinocytes [46] and murine K38 keratinocytes [59] were cultured in high glucose DMEM media (GIBCO 11965-092) supplied with penicillin and streptomycin (GIBCO 15140-122 ...
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bioRxiv - Bioengineering 2020Quote: ... All T cells were expanded in complete T-cell medium and fed interleukin (IL)-2 (50 U/mL; Life Technologies) and IL-15 (1 ng/mL ...
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bioRxiv - Bioengineering 2020Quote: ... and media for M(IL4/IL13) stimulation consisted of RPMI 1640 with 20 ng/ml interleukin 4 (IL-4; Invitrogen) and 20 ng/ml interleukin 13 (IL-13 ...
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bioRxiv - Immunology 2023Quote: ... After a 2 day culture with interleukin (IL)-21 and irradiated mouse fibroblast (L cells) expressing CD40-ligand in DMEM F12 media (Gibco), the B cells were immortalized by retroviral transduction with BcL6 ...
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bioRxiv - Microbiology 2022Quote: IL-8 and IL-1β cytokines were quantified by ELISA using the Rabbit IL-1 beta ELISA Kit and Rabbit IL-8 ELISA Kit (Invitrogen). Aliquots of 2 mL of lung homogenate shred in saline solution were centrifuged during 30 min at 10 000 rpm and 4°C ...
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bioRxiv - Genomics 2019Quote: Two µg of high quality total RNA was used to synthesize cDNA using high capacity cDNA reverse transcription kit (Applied Biosystems, USA) as per the instructions of the manufacturer and described in Paul et al ...
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bioRxiv - Genomics 2024Quote: ... followed by qualitative and quantitative analysis using an Agilent 4200 TapeStation High Sensitivity D5000 ScreenTape and Qubit 1X dsDNA High Sensitivity Kit (Thermo Fisher Scientific). The cDNA was enzymatically sheared to a target size of 200-300 bp ...
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bioRxiv - Microbiology 2023Quote: ... viral RNA was reverse transcribed using Superscript III One-Step RT PCR Kit with Platinum® Taq High Fidelity enzyme followed by amplification using Platinum® PCR SuperMix High Fidelity (Invitrogen). A 2200bp amplicon spanning gag and protease was generated by nested PCR as detailed in Supplementary Table 1 ...
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Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: ... iPSCs were grown feeder-free using the Essential 8 Flex Medium kit (Thermo Fisher Scientific) on Vitronectin (VTN-N ...
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bioRxiv - Genetics 2020Quote: ... The temperature sensitive collection was maintained in 1536-format on YPD plates containing 200 mg/L geneticin (G418; Invitrogen). The SGA query strain was maintained on YPD plates containing 100 mg/L nourseothricin-dihydrogen sulfate (NAT ...
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bioRxiv - Microbiology 2021Quote: ... for 12 hours was measured using a fluorescent membrane potential sensitive dye called bis-(1,3-dibutyl barbituric acid)-trimethylene oxonol (Invitrogen). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... cell membranes were loaded with the voltage-sensitive dye D-2- ANEPEQ (JPW1114, 0.2 mg/mL, Invitrogen, Carlsbad, CA) for 30 minutes using a first patch clamp recording and then re-patched a second time with dye free solution as previously described (Canepari et al ...
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bioRxiv - Bioengineering 2020Quote: ... Cells in culture washed with PBS and the media was replaced by Ca2+-sensitive Fluo-4 AM (Life Technologies) solution ...
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bioRxiv - Immunology 2022Quote: ... The bacteria were then centrifuged and resuspended in Sodium carbonate buffer (0.1 M, pH 9.0) for an additional staining step with the pH-sensitive dye CypHer5E (Fisher scientific). This was used at a concentration of 7 μg/ml in a volume of 1.5 ml for 2h at room temperature under gentle rotation ...