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Citations for Thermo Fisher :
851 - 900 of 7029 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Phase microscopy images were taken using the EVOS FL Auto Imaging System (Thermo Fisher) at 20X zoom.
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bioRxiv - Developmental Biology 2023Quote: ... The aqueous phase was extracted and precipitated overnight at −20°C using glycoblue (ThermoFisher) and isopropanol followed by centrifugation for 30 minutes at 4C at 10,000 RPM ...
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bioRxiv - Biochemistry 2023Quote: ... and fractionated with a Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific) into eight fractions according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Glycans were separated from peptides by reversed-phase chromatography through a C18 cartridge (ThermoFisher) and dried in a lyophilizer (LabConco ...
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bioRxiv - Cell Biology 2024Quote: ... and release into S phase in the prescence of EdU (Invitrogen; C10637, 0.5 µM) for 18-22 hours ...
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bioRxiv - Cell Biology 2024Quote: ... phase-contrast images were captured by EVOS FL Cell Imaging System (Thermo Fisher scientific) and bright-field images were captured by KEYENCE Fluorescence Microscope BZ-X800 (Keyence ...
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bioRxiv - Molecular Biology 2024Quote: The tryptic peptides were separated by reverse phase HPLC (Thermo Fisher Scientific Ultimate 3000) using a Thermo Fisher Scientific PepMap RSLC C18 column (2µm tip ...
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bioRxiv - Microbiology 2024Quote: ... The tryptic peptides were desalted using solid-phase extraction plates (ThermoFisher Scientific, SOLAµ™) and then freeze-dried for storage ...
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bioRxiv - Microbiology 2024Quote: ... The aqueous mobile phase consisted of 0.5% of formic acid (Fisher Scientific A117-50). The isocratic elution was achieved with Waters Acquity Premier HSS T3 Column ...
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bioRxiv - Immunology 2024Quote: Phase separation in cells lysed in 1 mL TRIzol Reagent (ThermoFisher cat. no. 15596018) was induced with 200 µL chloroform ...
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bioRxiv - Neuroscience 2024Quote: ... The organic phase was rated and evaporated by Savant Speedvac spd111v (Thermo Fisher Scientific) at RT for 1-2 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Contamination was monitored by phase-contrast microscopy and mycoplasma detection kits (Thermo Fisher #M7006).
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bioRxiv - Microbiology 2024Quote: 1µg of peptides was separated by an online reversed-phase (RP) HPLC (Thermo Scientific Dionex Ultimate 3000 RSLC nano LC system ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were directly loaded onto reversed-phase trap-column (Acclaim PepMap 100, ThermoFisher Scientific) and eluted in backflush mode ...
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bioRxiv - Cell Biology 2024Quote: ... connected in-line to a 15cm C18 reverse-phase analytical column (ThermoFisher EasySpray ES904), with 100% Buffer A (0.1% Formic acid in water ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were desalted on R2C8 and R3C18 resin reversed-phase columns (Thermo Fisher Scientific), washed with 0.1% TFA and eluted with 60% acetonitrile/0.1% TFA before drying under vacuum centrifugation and resuspending in 50 mM TEAB buffer.
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bioRxiv - Neuroscience 2024Quote: ... Samples were desalted by micropipette C-18 solid-phase extraction tips (StageTip, Thermo Scientific) prior to LC-MSMS analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... Basic pH reverse-phase fractionation was conducted on an Ultimate 3000 UHPLC system (ThermoFisher) equipped with a 2.1 mm × 15 cm ...
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bioRxiv - Cancer Biology 2024Quote: ... and Mobile phase B consisted of 99% LC/MS grade acetonitrile (A955, Fisher Scientific), 1% LC/MS grade water ...
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bioRxiv - Cancer Biology 2024Quote: ... and mobile phase B consisted of 90% LC/MS grade isopropanol (A461, Fisher Scientific), 8% LC/MS grade acetonitrile ...
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bioRxiv - Cell Biology 2021Quote: ... After the final wash the beads were resuspended in 100 μL of 50 mM ammonium bicarbonate (Thermo Fisher Scientific) and the proteins on the beads were reduced with 10 mM DTT for 30 minutes at room temperature and alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... PolyA RNA was ethanol precipitated with 2.5 M Ammonium Acetate and 70% ethanol in a solution containing 50 μg/ml GlycoBlue Co-precipitant (AM9515, Invitrogen). RNA was resuspended in 10 μl and fragmented with 10x Fragmentation Buffer (AM8740 ...
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bioRxiv - Cell Biology 2022Quote: ... After alkylation, beads were washed with 50 mM ammonium bicarbonate (Applichem, A3583) and incubated with Trypsin (Thermo Scientific,25247) at 37°C overnight (14-16 hours ...
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bioRxiv - Biophysics 2022Quote: ... all samples were immediately buffer exchanged into an nMS-compatible solution (150 mM ammonium acetate, pH 7.5, 0.01% Tween-20) with a 40 kDa MWCO (ThermoFisher Scientific). For nMS analysis ...
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bioRxiv - Molecular Biology 2020Quote: Purified PDX complexes at 0.2-0.4 mg/mL concentrations (10 µL each in 1xTBS with 4 mM DTT) were buffer exchanged into 200 mM ammonium acetate (pH 6.7) using Zeba 75 µL microspin columns from Thermofisher. Proteins were then loaded into pulled glass capillaries (GlassTip™ ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 µl of an isolation buffer (50 mM ammonium bicarbonate, 1% SDS, 1x Protein Inhibitor Thermo Fisher Scientific, #78430) were added to each tube containing the pellet ...
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bioRxiv - Cell Biology 2023Quote: ... the RNA extracted using the TRIzol method was further purified using the ammonium acetate precipitation method (Ambion, Catalog: AM9070G). The quality of RNA was assessed using Tapestation from Agilent Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... sample was buffer exchanged into 100 mM ammonium acetate using ZebaTM spin column (7 kDa MWCO, Thermo Fisher Scientific). Sample was loaded into Au/Pd-coated borosilicate emitters (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Red blood cells (RBCs) were then lysed by resuspending cells in ammonium-chloride-potassium (ACK) lysis buffer (Thermo Fisher) for 2 min ...
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Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availabilitybioRxiv - Cell Biology 2023Quote: ... Polyacrylamide gel solutions were prepared by adding 99.4% v/v of polymer solution (of corresponding stiffness) with 0.5% v/v ammonium per-sulfate (ThermoFisher Scientific) and 0.1% v/v TEMED (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... for 5 minutes and pellets were resuspended in Ammonium-Chloride-Potassium (ACK) lysing buffer (Thermo Fisher Scientific, Catalog # A1049201) to lyse RBC (erythrocytes removal performed at R.T ...
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bioRxiv - Cell Biology 2024Quote: ... MS was removed and 90 μL of MS was added together with ammonium persulfate (APS, 17874, Thermo Fisher Scientific) and tetramethylethylenediamine (TEMED ...
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bioRxiv - Molecular Biology 2024Quote: ... We discarded the supernatant and resuspended the cell pellet in 1mL of Ammonium-Chloride-Potassium (ACK) lysis buffer (Thermofisher) and incubated for 30 seconds at room temperature to lyse erythrocytes ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10 µL tetramethylethylenediamine (TEMED) and 150 µL 10 wt% ammonium persulfate (APS) cast into a mini cassette (ThermoFisher Novex). The percent urea in the gel can be adjusted depending on the application ...
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bioRxiv - Molecular Biology 2024Quote: ... according to the manufacturer’s protocol followed by a precipitation step with 1 volume of 5M ammonium acetate (Invitrogen, AM9070G) and 2.5 volumes of 100% ethanol ...
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bioRxiv - Biophysics 2024Quote: The RNAP samples were buffer-exchanged into nMS solution (500 mM ammonium acetate, pH 7.5, 0.01% Tween-20) using Zeba microspin desalting columns (Thermo Scientific) with a 40-kDa MWCO 73 ...
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bioRxiv - Cell Biology 2020Quote: ... The Superscript II RT mix also contained: 0.5ul 200U/ul Superscript II reverse transcriptase (Life technologies), 2ul 5x Superscript II First-Strand Buffer (Life technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... the hybridoma was gradually transitioned to Protein Free Hybridoma Medium II (PFHM-II, Thermo Fisher Scientific) and cultured for several days until the medium turned acidic ...
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bioRxiv - Microbiology 2022Quote: ... equipped with a DAD (Impact II) or MWD (micrOTOF II)-3000 RS UV-detector (Thermo Fisher) and coupled to an Impact II or micrOTOF II electrospray ionization mass spectrometer (Bruker).
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bioRxiv - Developmental Biology 2021Quote: ... The synthesized cDNAs were then employed as templates for PCR amplification using the ChemQ Universal SYBR qPCR Master Mix (Vazyme, Q711-02-AA) and a StepOne Plus Real-Time PCR System (Applied Biosystems) in the following program ...
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bioRxiv - Molecular Biology 2021Quote: ... mESCs were washed with PBS (Welgene, LB004-02) and detached from the dishes by incubation with trypsin-EDTA (Gibco, 25300-054) at 37°C for 2 min ...
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bioRxiv - Neuroscience 2020Quote: ... flies were anesthetized on CO2 and then restrained inside of a cut 200 μL pipette tip (#02-404-423; Fisher Scientific) so that their head and proboscis were exposed while their body and tarsi remain restrained ...
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bioRxiv - Neuroscience 2020Quote: ... and electrophoresed at 130 V for 2 h with the NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific, Cat# NP0001-02). At the end of electrophoresis ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells were maintained in Dulbecco’s Modified Eagle’s medium (DMEM, the Media Preparation Core of MSKCC) supplemented with 10% fetal bovine serum (Gibco, catalog A31606-02). Approximately 1.5 × 106 cells were co-transfected (in a six-well dish ...
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bioRxiv - Physiology 2020Quote: ... Prx1-derived muscle cells were plated at 1000 cells per cm2 and induced with myogenic medium containing F10 (31550-02, Life Technologies), 2% horse serum (26050088 ...
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bioRxiv - Microbiology 2020Quote: ... Dharmacon) and siGENOME Non-Targeting siRNA #2 (D-001210-02-05, Dharmacon) were transfected with Lipofectamine 3000 Reagent (L3000001, Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... or siGRHL2 #2 (qPCR validation) (Dharmacon, #L-014515-02-0050) using 2.5 ug/mL of Lipofectamine 2000 (Thermo Fisher Scientific, #11668019) in Pen/Strep-free media.
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bioRxiv - Molecular Biology 2020Quote: ... ref.17-1318-02) before being subjected to SDS-PAGE gel electrophoresis (NuPage™ 4-12% Bis-Tris Gel Invitrogen, NP0322box) and semi-dry transfer to a nitrocellulose membrane (iBlot®2NC Mini Stacks Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... Atp6ap1/Ac45 (Dharmacon cat. #M-060210-01-0005) or Atp6ap2/Prr (Dharmacon cat. #M-063641-02-0005) using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... they were boiled for 10 min at 85°C before being loaded and separated in SDS–polyacrylamide gels 4–12% (NuPAGE Bis-Tris, ThermoScientific) in MOPS 1X buffer (NP0001-02, NuPAGE, Novex, Life Technologies). After migration ...