Labshake search
Citations for Thermo Fisher :
851 - 900 of 10000+ citations for 6H dibenz C E 1 2 oxaphosphorin 6 oxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Slides were either stained with 4’,6-diamidino-2-phenylindole (DAPI) FluoroPure grade (Invitrogen) and mounted using Fluoromount aqueous mounting medium (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2024Quote: ... ProLong® Gold antifade reagent with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen #P36931) was added to each slide ...
-
bioRxiv - Bioengineering 2024Quote: ... calcein AM and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2024Quote: ... samples were counterstained in 300 nM DAPI (4’,6-diamidino-2-phenylindole; D1306, ThermoFisher) diluted in blocking buffer for 2 hr at room temperature ...
-
bioRxiv - Cancer Biology 2024Quote: ... Nuclear counterstaining was performed using DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, #D1306, Invitrogen).
-
bioRxiv - Cancer Biology 2024Quote: ... Nuclear counterstaining was performed using DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, #D1306, Invitrogen).
-
bioRxiv - Biochemistry 2024Quote: ... cells were stained with 300 nM 4’,6-diamidino-2-phenylindole (DAPI; D1306, Invitrogen) for 5 min at RT ...
-
bioRxiv - Bioengineering 2024Quote: ... the samples were exposed to the E-cadherin monoclonal primary antibody (HECD-1; 1:2000; 13-1700, Invitrogen) in PBST supplemented with 1% BSA for 24 hours at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... anti-His×6 (mouse monoclonal, Invitrogen, 1:10,000-20,000), anti-OmpA (rabbit polyclonal ...
-
bioRxiv - Cancer Biology 2022Quote: ... HCC cell line was obtained from JCRB (Osaka, Japan) and maintained at 37°C and 5% CO2 in William’s E medium (ThermoFisher Scientific, Cat. # 32551) supplemented with 10% heat inactivated FBS ...
-
bioRxiv - Microbiology 2022Quote: ... Cell lysates were then incubated overnight at 4°C with pan-flavivirus anti-E antibody and protein G Sepharose beads (Thermo Fisher Scientific). Beads were washed three times and eluted with 2× SDS loading buffer before Western blot analysis.
-
bioRxiv - Biophysics 2022Quote: ... The viral genomic RNA and the RNA of the helper plasmids C and E were in vitro transcribed and capped using the mMESSAGE mMACHINE SP6 Transcription kit (Thermo Fisher Scientific). Quality of the RNA was assessed on a denaturing formaldehyde-agarose gel ...
-
bioRxiv - Biochemistry 2021Quote: ... RNA was precipitated overnight at −20°C by the addition of 1 volume of isopropanol and 2 μL of GlycoBlue (15 mg/mL, Invitrogen). RNA was pelleted by centrifugation ...
-
bioRxiv - Microbiology 2021Quote: ... The 5’ and 3’ fragments (2 μg of each) were ligated for 1 h at 16°C by T4 DNA ligase (Invitrogen) and purified by phenol-chloroform extraction ...
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen) in solution with PBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1 mM DTT) at 37°C for 2 h followed by incubation with 0.75 U UDG (EN0361, Thermo Fisher Scientific) in 1x UDG buffer at 37°C for 45 min ...
-
bioRxiv - Immunology 2022Quote: ... and reduced at 37°C with 1:125 dilution of Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (ThermoFisher, Waltham, MA, USA) for 30 minutes ...
-
bioRxiv - Cancer Biology 2021Quote: ... ionomycin (1 μg/ml) and monensin (2 μg/ml) for 3-4 hours at 37°C in complete IMDM medium (Gibco). Viability staining was performed using the fixable viability dye eFluor780 ...
-
bioRxiv - Cancer Biology 2020Quote: Ex vivo cultures from primary tumor explants were generated by mechanical dissociation and incubation for 1 hour at 37°C with a solution of collagenase IV/dispase (2 mg/mL) (Invitrogen), resuspended in DMEM (Lonza ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... RT mixtures were incubated at 52°C for 2 min before addition of 1 μL (200 U) of Superscript III Reverse transcriptase (Invitrogen) and reactions were incubated at 55°C for 2 hr ...
-
bioRxiv - Molecular Biology 2022Quote: ... and then treated with 0.4 mg/ml proteinase K (1-2 hour, 50°C) before addition of 25 μM Sytox Green (ThermoFisher Scientific). Cells were then sonicated and DNA-content was analysed for >10,000 events on either a BD Biosciences FACScan Analyzer or an Attune NxT flow cytometer ...
-
bioRxiv - Microbiology 2023Quote: ... Total RNA was DNase digested for 30 min at 37°C using 1 µl TURBO DNase (2 U/µl) (Invitrogen). A 500-ng aliquot of the isolated RNA was converted to cDNA with the iScript Advanced Kit (Bio-Rad ...
-
bioRxiv - Genetics 2023Quote: Samples were then incubated at 37 °C for 1 h after addition of 2 μl of RNase Cocktail Enzyme Mix (Ambion). Then 40 μg of Proteinase K (Roche ...
-
bioRxiv - Biochemistry 2022Quote: ... The filtrate was then incubated for 1 h rotating at 4 °C with 2 mL Ni-NTA resin (Thermo Fisher) per 1.5 L of expression culture ...
-
bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated o/n at with rotation at 4°C with 2 µg IP antibody and for 1 h with Dynabeads Protein G (Invitrogen). Alternatively ...
-
bioRxiv - Cancer Biology 2023Quote: Ex vivo cultures from primary tumor explants were generated by mechanical dissociation and incubation for 1 hour at 37°C with a solution of collagenase IV/dispase (2 mg/ml) (Invitrogen), resuspended in DMEM (Lonza ...
-
bioRxiv - Biochemistry 2024Quote: ... 8 µL of 2.5X MaP Buffer was added and incubated at 42°C for 2 minutes before addition of 1 µL SuperScript II Reverse Transcriptase (ThermoFisher) and incubation at 42°C for 3 hours ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were centrifuged at 500 ×g for 5 minutes at 4°C and washed and resuspended with ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The transfection complex and BxPC3 cells and PNAC-1 cells were then mixed together and seeded in 6-well (2 x 105 cells/well) cell culture plates (Nunc, Rochester, NY, USA) and cultured at 37°C for 24 hours ...
-
bioRxiv - Immunology 2022Quote: PECs were harvested from untreated wild-type B6 and Nr4a1-/- mice and allowed to adhere to plastic wells (6-well plates, 2 × 106 cells/well) for 1 hour in AIM V serum free medium (ThermoFisher Scientific, Waltham, MA). Non-adherent cells were washed off with PBS and the adherent cells were cultured for 3-hrs in complete DMEM + 10% FBS medium containing LPS (1 μg/ml in PBS ...
-
bioRxiv - Immunology 2019Quote: ... Hemocytes nuclei were then stained by incubating with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Thermo Fisher Scientific, Rockford, IL, USA) dissolved in PBS and washed thrice with PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were transfected for 6 or 24 hours with Imp1-targeting siRNAs (siRNA #1: s155193, or siRNA #2: s155193. Both from Ambion, Thermo Fisher Scientific).
-
bioRxiv - Bioengineering 2023Quote: ... for 30 seconds at room temperature followed by 15 min staining with 1 µg/mL 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, cat. no. D1306) before microscopic imaging ...
-
bioRxiv - Cell Biology 2021Quote: ... rat anti-E-cadherin (131900; Invitrogen), mouse anti-GM130 (610822 ...
-
bioRxiv - Cancer Biology 2022Quote: ... anti E-cadherin (Invitrogen, 13-1900), anti N-cadherin (BD Transduction Laboratories ...
-
bioRxiv - Developmental Biology 2021Quote: ... rat anti-E-cadherin (Thermo Fisher) and Armenian hamster anti-Pecam1 (Bio-Rad ...
-
bioRxiv - Cancer Biology 2021Quote: ... composed of William’s medium E (Gibco), 12 mM Nicotinamide (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2022Quote: ... 150 μl buffer E (Invitrogen (1861754) buffer with 1 mM MgCl2 ...
-
bioRxiv - Biochemistry 2023Quote: ... and 2mM DTT) for 30 min at 4 °C and analyzed on 6% TBE gels (Life Technologies) at 4 °C ...
-
bioRxiv - Molecular Biology 2021Quote: Approximately 60 WT and MafAS64F/+ islets from at least 6 female and male mice were analyzed with the ratiometric calcium indicator fura-2-acetoxymethylester (Fura-2 AM) (Life Technologies). Islets were maintained in 5 mM glucose for 30 min prior to measuring 11 mM glucose-induced calcium oscillations and depolarization-activated calcium influx with 30 mM KCl ...
-
bioRxiv - Cell Biology 2024Quote: ... the conversion of non-fluorescent 2’,7’-bis-(2- carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF AM) (Invitrogen, Waltham, MA) into a pH sensitive fluorescent indicator by the intracellular esterase was used to measure the pH ...
-
bioRxiv - Bioengineering 2022Quote: ... 1% N-2 (Thermofisher), 2% B-27 (Thermofisher ...
-
bioRxiv - Microbiology 2023Quote: ... 1× N-2 (Gibco), 50 ng/ml of mouse EGF (RnD) ...
-
bioRxiv - Cell Biology 2020Quote: ... Ileal H&E sections were then imaged at 10X with an EVOS FL Auto 2 Cell Imaging System (Invitrogen, CA) to measure extent of ileal involvement of each NEC Grade ...
-
bioRxiv - Immunology 2021Quote: ... virus-infected cells were detected using an in-house rabbit polyclonal antibody specific for SARS-CoV-2 N/M/E followed by horseradish peroxidase-conjugated goat anti-mouse IgG (Invitrogen). TCID50 was calculated using the Reed-Muench method.
-
bioRxiv - Cancer Biology 2020Quote: ... Reactions were amplified for 24-27 cycles and then size selected using 2% E-Gel EX Size Select gels (Invitrogen). Libraries were quality checked using Agilent 2100 bioanalyzer (Agilent) ...
-
bioRxiv - Microbiology 2020Quote: ... 5 × 106 HEK 293T cells were co-transfected with 6 μg each of pNL4-3.Luc.R-E- and recombinant SARS-CoV-2 S plasmids (pS-D614 or pS-G614) using Lipofectamine 3000 Transfection Reagent (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2020Quote: ... and used to pool samples equally followed by size selection (200–600 bp range) on an E-gel 2% SizeSelect gel (Invitrogen). The size-selected library was precipitated and resuspended in 20 μl of water followed by Ampure XP bead (ratio 1:1.8 ...
-
bioRxiv - Microbiology 2022Quote: ... The SARS-CoV-2 E sgmRNA standards were generated by in-vitro transcription of the generated SARS-CoV-2 E sgmRNA cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by the MEGAclear Transcription Clean-Up Kit ...
-
bioRxiv - Genomics 2020Quote: ... DNA bands with the correct amplicon sizes were extracted via gel extraction and purification using 2% agarose precast gels (E-Gel EX, Invitrogen) and gel imager (E-Gel Safe Imager connected to E-Gel iBase ...