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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for 3 3 Dimethyl 6 oxa 3 phosphoniabicyclo 3.1.0 hexane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... or a 3-8% Tris Acetate gel (Invitrogen). Proteins were wet transferred onto a PVDF membrane (Millipore ...
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bioRxiv - Plant Biology 2024Quote: ... on the Qubit 3 Fluorometer (Q33216; Life Technologies). Total Rubisco content was measured by 14C-CABP binding as previously described (Ruuska et al. ...
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bioRxiv - Plant Biology 2024Quote: ... and Qubit 3 Fluorometer (Thermo Fisher, Waltham, USA), respectively.
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bioRxiv - Genetics 2024Quote: ... Cell number was determined by Countess 3 (Invitrogen) and was used to normalize oxygen consumption rate in pmoles/min.
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bioRxiv - Bioengineering 2024Quote: ... 4 × 10−3 M GlutaMAX supplement (35050061, Gibco), 2 × 10−4 M L-cystine (C7602 ...
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bioRxiv - Pathology 2024Quote: ... and QuantStudio 3 real-time PCR system (ThermoFisher). Primers were used from the SV40 polyadenylation site ...
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bioRxiv - Molecular Biology 2024Quote: ... and 3 U FastAP enzyme (Thermo Fisher Scientific) were added in a total volume of 50 µl and incubated for 20 min at 37 °C shaking at 1,200 rpm ...
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bioRxiv - Microbiology 2024Quote: For cultures stained with DiBAC4(3) (Invitrogen, Thermofisher), 0.5 μl dye was added to 100 μl of cells and the mixture was incubated at 30°C with shaking at 800 rpm for 5 minutes ...
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bioRxiv - Microbiology 2024Quote: For cultures stained with DiBAC4(3) (Invitrogen, Thermofisher), 0.5 μl dye was added to 100 μl of cells and the mixture was incubated at 30°C with shaking at 800 rpm for 5 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 3 U furin (Thermo Fisher, Waltham, MA, USA) was mixed with 100 µM of substrate in a total volume of 100 µl reaction buffer (100 mM HEPES pH7.5 + 1 mM CaCl2 + 0.5% Triton X-100 ...
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bioRxiv - Microbiology 2024Quote: ... using the QuantStudio 3 thermal cycler (Applied Biosystems). The results were analyzed using the QuantStudio Design & Analysis v1.5.2 software with the 2−ΔΔCt method (52) ...
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bioRxiv - Immunology 2024Quote: ... using the QuantStudio 3 (Applied Biosystems, CA, USA) machine ...
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bioRxiv - Plant Biology 2024Quote: Qubit 3 Fluorymetr (Invitrogen, Carlsbad, CA, USA; Q33216)
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bioRxiv - Cell Biology 2024Quote: ... Amp-3 and HRP-tagged probe (ThermoFisher Inc) for 30 ...
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bioRxiv - Microbiology 2024Quote: ... 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI; Invitrogen) while Ud23 and Ud26 IAV were labeled with a different lipophilic dye with a longer excitation and emission wavelength ...
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bioRxiv - Physiology 2024Quote: ... using the Quant Studio 3 system (Applied Biosystems). qPCR reactions were prepared in a final volume of 20 μl containing 2 μl cDNAs ...
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bioRxiv - Physiology 2024Quote: CellEvent Green Caspase 3/7 dye (ThermoFisher Scientific) was added to cells in a glass bottom plate with +/- 3-oxo in phenol-free DMEM ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... qPCR was run on QuantStudio 3 (Applied Biosystems) using the glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Neuroscience 2024Quote: ... 3 µg/ml BrdU (Invitrogen; Cat:00-0103) was added and incubated overnight ...
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bioRxiv - Neuroscience 2024Quote: ... performed using a QuantStudio 3 (Thermo Fisher Scientific). EIF2A was used as the housekeeping gene ...
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bioRxiv - Genomics 2024Quote: ... (3) protein A magnetic Dynabeads (Thermo Fisher, 10008D) were used.
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bioRxiv - Immunology 2024Quote: ... rabbit anti-caspase 3 (710431, Invitrogen, 1:100), rabbit anti-PDL1 (GTx57193 ...
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bioRxiv - Bioengineering 2024Quote: ... 3 µg Trypsin enzyme mix (Thermo Fisher Scientific) was added to sample and incubated overnight (17 hours ...
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bioRxiv - Bioengineering 2024Quote: ... 3 kDa Dextran-Cascade Blue (Invitrogen, Cat.# D7132), was added to the vascular compartment of the Brain-Chip at 0.1 mg/ml ...
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bioRxiv - Systems Biology 2024Quote: ... or iBlot 3 (Thermo Fisher Scientific Cat# A56727) dry blotting system ...
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bioRxiv - Cancer Biology 2024Quote: ... with Countess 3 Automated Cell Counter (Life Technologies). For cell viability assays ...
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bioRxiv - Bioengineering 2024Quote: ... Anti-GAPDH (3:1000) monoclonal antibody (mouse) (Invitrogen) was used as a loading control to ensure equal protein loads across all sample lysates ...
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bioRxiv - Biochemistry 2024Quote: ... and 20 nM Topro-3 (ThermoFisher Scientific, T3605) were added according to the manufacturer’s guidelines ...
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bioRxiv - Physiology 2020Quote: ... and incubated with 100 mM 6-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (6-NBDG) (Life Technologies) in 10 nM Tris/HEPES buffer containing 150 mM KCl or 150 mM NaCl for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2021Quote: 3 μg of in vitro-transcribed RNA derived from the pCrPV-3 or chimeric clones was transfected into 3 × 106 S2 cells using lipofectamine 2000 reagent (Life Technologies) per the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... The amplicon of the-2.3etv2 promoter was synthesised from zebrafish genomic DNA with a forward primer 5’- TATAGGGCGAATTGggtaccTTCAGTAAGCAGACTCCTTCAATCA -3’ and a reverse primer 5’- AGCTGGAGCTCCAccgcggTTCGGCATACTGCTGTTGGAC -3’ by Phusion High-Fidelity DNA Polymerase (Thermo Scientific) as an insert for In-Fusion Cloning (Takara Bio ...
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bioRxiv - Immunology 2022Quote: ... and 0.75% w/v 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) in a hydrated 10K molecular weight cutoff dialysis cassette (Thermo Scientific). S ‘2P’ and HexaPro IMAC elution fractions were concentrated to ∼ 1 mg/mL and dialyzed three times into 50 mM Tris pH 8 ...
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bioRxiv - Microbiology 2022Quote: ... The region of recombination (VP1 to 2C) was amplified using primers PV3-F (5′-GCAAACATCTTCCAACCCGTCC-3′) and PV1-R (5′-TTGCTCTTGAACTGTATGTAGTTG-3′) and Taq polymerase (Life Technologies) with an initial denaturing at 94°C for 3 min ...
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bioRxiv - Microbiology 2022Quote: ... 16S rRNA gene was amplified using primers 8F (5’-AGAGTTTGATCCTGGCTCAG-3’) and 1492R (5’-GGTTACCTTGTTACGACTT-3’) by colony PCR (Vaishnava et al., 2011) using DreamTaq Master Mix (ThermoFisher Scientific) and 0.2μM primers ...
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bioRxiv - Neuroscience 2022Quote: ... cells were loaded in microglia differentiation medium with 3 μM Fluo-4 AM and 3 μM Fura-Red AM (Molecular Probes) in the presence of Pluronic Acid F-127 (Molecular Probes ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... using the oligonucleotides WPRE-F 5’-TGCTTCCCGTATGGCTTTCAT-3’ and WPRE-R 5’-CAGCAAACACAGTGCACACC-3’ as primers and SYBR Select Master Mix (ThermoFisher Scientific). The measurements were performed with a CFX384 instrument (Biorad ...
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bioRxiv - Molecular Biology 2020Quote: ... in 1.5ml of SD/-Leu/-His liquid medium (plus 3 mM 3-AT) were incubated in a 12-well microplate (Thermo Scientific) at 30°C for 96 hours (h) ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: The wild-type copy of xopX and its 14-3-3 protein binding motif mutants were cloned by Gateway cloning (Invitrogen, California) from the pENTR clones to the BiFC vector pDEST-VYCE(R)GW carrying the C-terminal region of the Venus Fluorescent Protein (VFP ...
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bioRxiv - Immunology 2021Quote: ... was added as the secondary antibody at a 1:2000 dilution for 1 h at 37C, followed by adding TMB (3, 3, 5, 5’-tetramethylbenzidine) peroxidase substrate (Thermo Scientific) for about 15 min ...
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bioRxiv - Cancer Biology 2020Quote: ... using the following primers c=myb-F 5’-CCAAGTCAGGAAAACGCCACCTCG-3’ and c-myb-R 5’-GCTGTTGTTTAGCGGAGTTGGGCT-3’ and cloned into the dual promoter vector pCRII-TOPO (Life Technologies). The pCS2:runx1 probe was a gift from Leonard Zon ...
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bioRxiv - Genomics 2020Quote: ... using forward primer (5’-TGATTATCGACATCCCGTCA-3’) and reverse primer (5’-GTCTGGAATCTCATAGGTAG-3’) and run on an ABI 7500 thermocycler (Applied Biosystems). Primer specificity and capture temperature were determined by melt curve analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... The purified RNA obtained from IVT was labelled with biotin at the 3’end using the PierceTM RNA 3′End Desthiobiotinylation Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... RNA was desiccated to 3 μl and denatured for 3 minutes at 72 °C in the presence of 2.4 mM dNTP (Invitrogen Carlsbad, USA), 240 nM dT-primer* (Metabion ...
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bioRxiv - Neuroscience 2020Quote: Genomic DNA in the vicinity of the rs7143400 was amplified by PCR using the following primers 5’-GGTTGGGTGTGAATAGGAAT-3’ and 5’-TGCATGCCTGATTTATTTGG-3’ before digestion with Tsp45I enzyme (Thermo Scientific). Finally ...
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bioRxiv - Microbiology 2020Quote: ... Asaia1 (5’-AGC ACC AGT TTC CCG ATG TTA T-3’) and Asaia2 (5’-GAA ATA CCC ATC TCT GGA TA-3’) labeled with Alexa Fluor® 555 (Invitrogen). Tissues were visualized using Nikon ECLIPSE IVi microscope connected to a Nikon DIGITAL SIGHT DS-U3 digital camera.
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bioRxiv - Microbiology 2021Quote: ... Resulting colonies were screened for plasmid presence by PCR of a portion of the pESC-URA-lpt1 plasmid (Forward primer: 5’-TTGGAAACAGCTCCAAATCC-3’, Reverse primer: 5’ CCCAAAACCTTCTCAAGCAA-3’; ordered from ThermoFisher Oligos) and preserved as glycerol stocks.
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bioRxiv - Immunology 2020Quote: ... and 0.75% w/v 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) in a hydrated 10K molecular weight cutoff dialysis cassette (Thermo Scientific). S-2P IMAC elution fractions were concentrated to ~1 mg/mL and dialyzed three times into 50 mM Tris pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: Human cavin3 Stealth siRNAs (set of 3-HSS174185, 150811, 150809) and Human BRCA1 Stealth siRNAs (set of 3 - HSS101089, 186096, 186097) were purchased from Life Technologies Australia Pty Ltd ...
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bioRxiv - Cancer Biology 2020Quote: Apoptosis induction was determined by activation of Caspase 3 and 7 using the CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen).H460 cells were plated at 5 × 103 cells/well in black 96 well plates with clear bottoms (Costar ...
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bioRxiv - Developmental Biology 2022Quote: ... were retrotranscribed from either 5’-CATGCTGCTGGTGGGTGTGCT-3’ or 5’-CCATAAAGCACCGGTGAGCAGAA-3’ endoglin specific reverse oligonucleotides (500 nM) using RevertAid H minus reverse Transcriptase (Thermo Scientific) 10 U/μl in 1X RevertAid H minus Buffer supplemented with 2 U/μl RNAse OUT (Thermo Scientific) ...