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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Devices were washed with PBS for 4-6 hrs and subsequently incubated with Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) (ThermoFisher cat# A32728, 1:300) and Hoechst 33342 (Sigma-Aldrich cat# B2261 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... After several wash and centrifugations, nuclei were suspended in a solution (phosphate=buffered saline containing 1% 4’, 6-diamidino-2-phenylindole (DAPI) (Molecular Probes, Eugene, OR, USA), and nuclei density was determined in a hemocytometer ...
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bioRxiv - Neuroscience 2024Quote: ... and the nuclei were stained in 0.05 M PBS solution containing 1 mg/ml polyvinylpyrrolidone and 0.5 μl/ml 2-(4-amidinophenyl)-1H-indole-6-carboxamidine (DAPI; Thermo Fisher Scientific, Waltham, MA, USA). The coverslips were rinsed in distilled water for 5 minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... ToPro-3 1:1000 and TMRM 1:100,000 (Invitrogen) added and incubated for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... cells were loaded in microglia differentiation medium with 3 μM Fluo-4 AM and 3 μM Fura-Red AM (Molecular Probes) in the presence of Pluronic Acid F-127 (Molecular Probes ...
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bioRxiv - Cell Biology 2022Quote: ... Blocks of tissue of ~3×3×4 cm (depth×width×height) were cut and prepared for sectioning using a Vibrating Blade Microtome (Thermo Fisher) at a thickness of 500μm ...
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bioRxiv - Bioengineering 2023Quote: Passage 3 and passage 4 cells from 8 donors (4 male and 4 female) were expanded in T175 flasks (Thermo Fisher Scientific, Hampton, New Hampshire USA) and were cultured until passage 5 (p3-5 and p4-5 ...
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bioRxiv - Biochemistry 2024Quote: ... dsRNA complex (2:1 molar ratio) were cross-linked with 30 μg 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Fisher Scientific, EDC: protein = 3:1 w/w) in the presence of 66 μg N-hydroxysulphosuccinimide (NHS ...
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bioRxiv - Physiology 2020Quote: ... 6-diamidino-2-phenylindole (DAPI, 1:25000, Thermo Fisher) diluted in PBS and mounted with Prolong Gold Antifade Medium (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... anti-His×6 (mouse monoclonal, Invitrogen, 1:10,000-20,000), anti-OmpA (rabbit polyclonal ...
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bioRxiv - Neuroscience 2024Quote: ... 6-diamidino-2-phenylindole (DAPI) (1:10000, Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... 5-(and-6)-Carboxy SNARF™-1 (C1270, Invitrogen), was dissolved to a final concentration of 10 mM in ddH2O ...
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bioRxiv - Microbiology 2021Quote: ... Reverse 5’-CGA AGG TGT GAC TTC CATG-3’) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). A standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
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bioRxiv - Developmental Biology 2022Quote: ... On day 3-9 cells were fed daily with Essential 6 medium (E6, #A1516401, Thermo Fisher Scientific) in the presence of 100 nM LDN193189 (#72142 ...
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bioRxiv - Immunology 2020Quote: ... plates were incubated with 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) substrate (ABTS, Thermo Fisher Scientific) for 15 min at RT shielded from light and absorbance was measured at optical density (OD ...
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bioRxiv - Molecular Biology 2023Quote: U2OS 2-6-3 cells (Shanbhag et al., 2010) were cultured in McCoy’s 5A (Modified) Medium (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... 6 and 8 weeks of maturation were loaded with 2.5 µM Fluo-3-AM (Molecular Probes, #F1242) dissolved in differentiation medium for 30 min at 37 °C and at 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... 3 and 6 h and stained live with 100 nM Lysotracker red DND-99 (Thermo Fisher, L7528) for 1.5 h before ending the treatment ...
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... and immersed and incubated in the dark in staining solution 1 mM 5-bromo-4-chloro-3-indolyl-β-D-glucuronicacid (X-Gluc, Thermo Scientific), 50mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2021Quote: ... One microgram of peptides in a volume of 1-4 µL was loaded onto the Acclaim µ-Precolumn (0.5 mm х 3 mm, 5 µm particle size, Thermo Scientific) at a flow rate of 10 µL/min for 4 min in an isocratic mode of Mobile Phase C (2% acetonitrile (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were incubated with secondary antibody for 3 nights at 4°C with secondary antibodies at 1:500: goat anti-rabbit Alexa Fluor 488 (ThermoFisher #A-11008) and goat anti-mouse Alexa Fluor 647 (ThermoFisher #A-21236) ...
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bioRxiv - Cell Biology 2021Quote: ... The tissue was washed in warm PBS and incubated in pre-warmed Buffer 1 (PBS 93%, 0.5M EDTA 3%, RNA secure 4%, Thermo Fisher Scientific, Australia), at 37°C on a shaker for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed with ice-cold PBS-BSA before secondary labeling for 1 h at 4°C in 3 mL1:200 PE-conjugated streptavidin (Thermo Fisher S866) to label for bound ACE2 ...
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bioRxiv - Microbiology 2020Quote: ... Pre-cleared lysates were further incubated at 4°C overnight with the indicated antibodies (1 to 3 μl) and protein A agarose or protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitates were washed three times with RIPA buffer (LSB ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed 4-5 times in PBS for 2 hours then incubated for 3-4 hours with secondary antibody (anti-rabbit Alexa Fluor 568; 1:1000; Invitrogen; Cat #A10042) and NeuroTrace Green Fluorescent Nissl Stain (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... 3 µL of 1 mM Fluo-4 AM (stock solution in DMSO) and 30 µL of PowerLoad Concentrate (100X, Thermo Fisher Scientific) were vortexed vigorously ...
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bioRxiv - Physiology 2023Quote: ... 100 ng pMD2 and 400 ng of each sgRNA CRISPR Cas9 lentivirus plasmid (plasmid amount rate 3:1:4) using Turbofect transfection reagent (Thermo Fisher Scientific). Lentiviral media was centrifuged once at 1500 x g for 3 minutes and the supernatant was collected ...
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bioRxiv - Biophysics 2023Quote: ... with a FuGene: DNA ratio of 3:1 using 4 μg of DNA in OptiMEM Reduced Serum Medium (ThermoFisher Scientific Catalog #11058021). The transfection medium was removed from cells ∼6–8 h post transfection ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies were incubated on slides in a the dark for 3 hours at room temperature or overnight at 4°C: GαM 488 (Invitrogen A21121, 1:1000), GαRb 546 (Invitrogen A11010 ...
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bioRxiv - Cell Biology 2023Quote: ... were plated into 12-well plate at a density of 3-4 × 105 cells in 1 mL per well in 1x DMEM (Gibco Cat# 11995065) plus 10% FBS (Gibco Cat# 16000044 ...
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bioRxiv - Immunology 2024Quote: ... The membrane was blocked with 5% non-fat milk for 3 hours and incubated overnight at 4°C with primary antibodies against COL1 (1:1000, Invitrogen, PA5-95137), COL3 (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in PBS with 30nM of 4’,6-diamidino-2-phenylindole (DAPI, Life Technologies). In each tube ...
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bioRxiv - Molecular Biology 2022Quote: ... and then the nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) for 5 min ...
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Single-cell protein analysis reveals metastable states during the transition to a sensory organ fatebioRxiv - Developmental Biology 2021Quote: ... Then they were stained with 0.5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI, Thermo Scientific), and mounted in Vectashield (Vector Labs) ...
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bioRxiv - Microbiology 2021Quote: ... and stained with 200 μl of 1.0 μg/ml 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen) diluted in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclei were counterstained using 5μg/ml of DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride; D1306, Invitrogen) added during secondary antibody incubation.
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bioRxiv - Cancer Biology 2021Quote: ... : DAPI Buffer (106mM MgCl2, 50 µg/mL 4’, 6-diamidino-2-phenylindole (DAPI, Invitrogen, Cat# D1306), 5mM Ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Physiology 2022Quote: ... CellTracker Green CMFDA Dye (C2925) and DAPI (4’,6-diamidino-2-phenylindole) (D1306) were from Invitrogen. EZ-Link Sulfo-NHS-LC-LC-Biotin (#21135 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Nuclei were then stained with 0.01% w/v 4’,6-diamidino-2-phenylindole dilactate (DAPI, Invitrogen) for 5 min and rinsed with ddH2O ...
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bioRxiv - Neuroscience 2022Quote: ... Non-viable cells were excluded by staining with 4’,6-diamidino-2-phenylindole (Thermo Fisher, D1306). Flow-cytometric data were analyzed using FlowJo software (BD Biosciences).
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were counterstained with 300nM 4’,6-diamidino-2-phenylindole dilactate (DAPI) (D1306, ThermoFisher Scientific, UK) for imaging assessment (Zeiss Axio observer Z1 microscope) ...
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bioRxiv - Immunology 2021Quote: ... however Live/Dead stain was not performed and instead 4’-6’-diamidino-2-phenylindole (DAPI, ThermoFisher) was added immediately prior to sorting for live/dead detection ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei were stained with 4′,6′-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng/ml, Molecular Probes). The sections were mounted using a fluorescence mounting medium (DAKO ...
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bioRxiv - Neuroscience 2020Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) and Leica standard immersion oil were obtained from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... All IF sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific, USA). Immunofluorescent images were acquired on an Olympus FV3000 microscope ...
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bioRxiv - Immunology 2023Quote: ... Coverslips were exposed to 200 ng/mL 4′,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific, 62247) for 2 min to label nuclei ...
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bioRxiv - Microbiology 2023Quote: ... IFN-γ IL-4 and IL-6 were measured by Mouse Uncoated ELISA Kit (Invitrogen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Gel surfaces were functionalized with sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino) hexanoate (Sulfo-SANPAH, Thermo Scientific) and covalently coated with 300 μg ml-1 collagen-I (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... anti-integrin β1 antibody and 4’,6-diamidino-2-phenylindole (DAPI) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Physiology 2023Quote: ... Sections were mounted in Antifade Mounting Medium with 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher) and mounted in Antifade Mounting Medium ...