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Citations for Thermo Fisher :
8901 - 8950 of 10000+ citations for Recombinant Human UBE2Z Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Plates were read using an MSD SECTOR Imager 2400 and resulting peptide concentrations were normalized to total protein in the cell lysate per well measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Data for each shRNA knockdown were additionally normalized to the average of control conditions for each parameter measured.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The pellet was resuspended in PBS/EDTA buffer and the protein concentration of the homogenate measured using the Pierce BCA protein assay kit (Thermo Scientific, USA). AChE activity was measured using the Ellman assay using acetylthiocholine as substrate and the indicator 5,5′-dithiobis-2-nitrobenzoic acid (DTNB ...
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bioRxiv - Neuroscience 2023Quote: ... was recombinantly expressed as an N-terminal Fc-fusion protein (Fc-hTfR1) in Expi293 suspension cells and purified by gravity flow with PierceTM Protein A Agarose (Thermo Fisher Scientific). HFCII probes were loaded with Fc-hTfR1 by thresholding to a 1 nm shift ...
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bioRxiv - Plant Biology 2024Quote: ... protein concentrations of fractions containing FtsZ1-2 and FtsZ2-1 were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... Protein content of the crude mitochondrial fraction was measured by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific catalog number 23225). Thirty µg of mitochondria protein was incubated at 25 °C in 850 µL reaction buffer (100 mM Tris-HCl ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentrations in the cytoplasmic and nuclear fractions were determined using a Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Cat. 23228). The isolated cytoplasmic and nuclear proteins were aliquoted and stored at -80°C for subsequent analysis.
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bioRxiv - Genomics 2024Quote: rox2RNA probes at 100 nM and hrp38 RNA probes at 50nM were incubated with MBP-tagged FL CLAMPWT protein or MBP-tagged FL CLAMPdelPrLD protein in REMSA binding buffer provided with the LightShift Chemiluminescent RNA EMSA kit (Thermo Scientific, USA) at room temperature for 30 min according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... a mass calibration was recorded to allow conversion between contrast to mass using a protein standard (Invitrogen NativeMark™ Unstained Protein Standard) with masses of 1236 ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the purified proteins was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, United States) as described previously (Choi et al. ...
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bioRxiv - Microbiology 2024Quote: ... Final protein concentration of the pooled elution fraction was determined with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA) before being used for in vitro acetylation.
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bioRxiv - Cell Biology 2023Quote: ... was added as a supplement to the lysis buffer and the protein concentration was measured using a Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2024Quote: Protein synthesis was assessed using the Click-iT® L-homopropargylglycine (HPG) Alexa Fluor 488 Protein Synthesis Assay Kit (Molecular Probes, USA), which measures newly synthesized proteins through quantification of the fluorescent HPG signal ...
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bioRxiv - Immunology 2024Quote: ... To measure the protein content of the lysate a Qubit 4 fluorometer was used using the Qubit protein assay kit (Thermo Fisher Scientific). Equal amounts of pre-cleared lysate samples were separated in 8 – 16% Mini protean-TGX stain free protein gel (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... and subcellular protein fractionation was performed on 1.0 x 107 cells using the Subcellular Protein Fractionation Kit for Cultured Cells (ThermoFisher Scientific, Waltham, MA). Protein concentrations were determined using bicinchoninic acid (BCA ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... and cytosolic and membrane proteins were extracted with the use of a Mem-PER Plus Membrane Protein Extraction Kit (Thermo Fisher, 89842).
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bioRxiv - Cell Biology 2024Quote: ... Following treatment cells were then transduced with Premo™ Autophagy tandem sensor Red Fluorescent Protein-Green Fluorescent Protein (RFP-GFP)-LC3B (Life Technologies, Invitrogen) and chloroquine was used as control ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration of each lysate was measured using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, CA, USA). The 2′-3′-cGAMP content were measured by 2′-3′-cGAMP ELISA kit ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration of each sample was determined by the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, CA, USA). Equal amounts of proteins (up to 20 μg ...
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bioRxiv - Plant Biology 2023Quote: ... The lysate was cleared by centrifugation (18,000 x g for 10 min at 25°C) and the protein content of the supernatant was determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific). Reduction ...
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bioRxiv - Microbiology 2023Quote: ... calcitrans salivary proteins by size with proteins visualised by Coomassie brilliant blue dye or silver staining (Pierce™ Silver Stain Kit, ThermoFisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... Whole cell lysates were centrifuged for 15 min at maximum speed at 4°C and protein concentrations measured from the supernatant with a Pierce BCA Protein Assay Kit (Thermo Scientific, 23227). 15 µg protein per lane were resolved on 8% SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 °C for 20 min and supernatants were transferred into french tubes before protein quantification using the Pierce BCA protein assay (Thermo Fisher Scientific). Lysate equivalent to 10-50 µg of proteins was mixed to 1× Laemmli buffer (50 mM Tris ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein content of all samples analyzed by SDS-PAGE was estimated using a Pierce bicinchoninic acid (BCA) protein assay (Thermo Scientific, Germany) using the 96-well plate protocol as recommended by the manufacturer.
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bioRxiv - Physiology 2023Quote: ... BAL cells were counted using a LUNA-II automated cell counter (Logos Biosystems) and BAL supernatant total protein was measured using a Pierce total protein assay (Thermo Scientific, #23225).
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bioRxiv - Cancer Biology 2023Quote: ... Peptide sequences (and hence protein identity) were determined by matching protein databases with the acquired fragmentation pattern by Sequest (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were removed and the protein concentration was quantified using the colorimetric assay by PierceTM BCA protein assay kit (ThermoFisher Scientific, 23225). Protein lysates were resolved using 10% SDS-PAGE gels and transferred to 0.45 µm PVDF membranes (Millipore ...
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bioRxiv - Immunology 2023Quote: ... they are displayed as area ratio and were normalized to protein content measured using Pierce TM BCA protein assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: Samples (input (eluted in 2× SDS buffer and thawed 15 minutes at 75°C) and IP proteins) were run in NuPAGE 4%–12% Bis-Tris Mini Protein Gel (10 wells) (ThermoFisher Scientific, NP0321BOX) at the voltage of 100V for 2.5 hours in a NuPAGE MOPS SDS Running buffer (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... Twenty-five microliter of the slurry was centrifuged and the supernatant was subjected to protein concentration determination using Pierce TM BCA protein assay Kit (Thermo Fisher Scientific). The protein slurry was diluted to 1 ng/µl with 100 mM Tris pH 8 ...
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bioRxiv - Physiology 2023Quote: At day 45 (± 2 days) protein was extracted from beating iPSC-CMs in protein lysis buffer (1 X Pierce™ RIPA buffer (Thermo Fisher); 1 X PhosSTOP cocktail (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifuging at 12.000 rpm for 5 min at 4°C and protein concentration determined using the Pierce™ BCA Protein Assay (Thermo Scientific 23225). Samples were prepared for SDS-Page in 1× NuPAGE™ LDS Sample Buffer containing 50mM DTT ...
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bioRxiv - Bioengineering 2023Quote: ... The amount of affibody conjugated to the polymer was quantified by dissolving 2-3 mg of HA-Nor-Ox-Affibody in PBS at 2% w/v and determining the protein concentration of the solution using a Pierce 660 Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... The membrane pellet was resuspended in the same buffer and the protein concentration was determined spectroscopically with the BCA protein assay kit (Thermo Fisher Scientific). The membrane fraction was supplemented with n-dodecyl-β-D-maltoside (DDM ...
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bioRxiv - Physiology 2023Quote: ... Tau protein was also labeled separately with Alexa Fluor 555 using a kit (Microscale protein labeling kit; ThermoFisher Scientific; Waltham, MA, USA) and by following the manufacturer instructions ...
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bioRxiv - Biochemistry 2023Quote: ... HM and IF pooled EV fractions were assayed for total protein according to manufacturer’s instructions (Micro BCA protein assay kit, Thermofisher Scientific, Brisbane, Australia) and a volume equivalent to 20 µg total protein was aliquoted for western blot (WB ...
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bioRxiv - Cell Biology 2022Quote: ... For LI-COR westerns: The proteins were electro-transferred to nitrocellulose membranes and then the total protein was stained using Ponceau (Thermo Fisher Scientific). The membrane was then blocked with LI- COR blocking buffer at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... MSC-EVs were not lysed in RIPA buffer and protein content was determined via the Micro BCA™ Protein Assay Kit (ThermoFIsher, 23235).
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bioRxiv - Genetics 2022Quote: ... A protein concentration of the tissue lysates was determined with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) and 3-10 µg of the total protein lysate/homogenate used per assay well in duplicates and repeated in three independent runs ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation at 17,000 xg for 5 min and total protein was quantified in the supernatant using the BCA protein assay (Thermo Scientific, Rockford, IL). SDS loading buffer (final concentrations of 100 mM Tris pH 6.8 ...
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Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: ... protein concentration was standardised to 20 μg/mL according to the protein quantification results tested by the Micro BCA protein assay kit (Thermo Fisher Scientific). Reduction was carried out using 10 μL TCEP was added followed by incubation at 37 °C for 45 min followed by alkylation with 55 mM iodoacetamide ...
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bioRxiv - Cell Biology 2023Quote: ... Lysate (2 mg/mL in 200 μL) was precleared with 20 μL of protein Pierce protein A/G magnetic beads (Thermo Fisher, 88802). 5 μg of anti-KEAP1 (Santa Cruz Biotechnologies ...
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bioRxiv - Neuroscience 2023Quote: ... The homogenates were cleared by centrifugation at 13,000 x g at 4°C for 25 min and the protein concentration in the collected supernatant was measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Protein extracts (20 µg of protein for each sample) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were then centrifuged for 15 min at 16,000 × g at 4°C and their protein concentrations were determined using a BCA Protein Assay Kit (#23227, Thermo Fisher Scientific, USA), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... the membrane preparations were solubilized with 1% DDM and the total protein content was measured using Pierce™ 660 nm Protein Assay Reagent (Thermo Scientific) against the BSA standard curve (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were then centrifuged at 10,000 rpm for 10’ at 4°C and the supernatant was collected to determine protein concentration using Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific). Before loading ...
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bioRxiv - Immunology 2023Quote: Supernatants were collected from cell cultures and protein extracts of lung tissue were obtained using T-PER Tissue Protein Extraction Reagent (ThermoFisher, Cat# 78510). The levels of mouse IL-5 and IL-13 were measured with DuoSet ELISA kits (DY405 and DY413 respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected after centrifugation for 10 min at 15 x 1000 g at room temperature and were used for protein quantification with BCA protein assay (Thermo Fisher, 23225).
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bioRxiv - Neuroscience 2023Quote: ... Lysates were then cleared by centrifugation and protein concentrations determined by Bradford Assay using a Peirce Protein Assay Kit (Thermo Scientific 23200). Equal amounts of protein were denatured in reducing sample buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptide sequences and hence protein identities were determined by matching protein databases with the acquired fragmentation pattern by the software program Sequest (Thermo Fisher Scientific)65 ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting supernatant was used for protein concentration determination using a Pierce 660 nm Protein Assay (Thermo Fisher Scientific, Waltham, MA, USA).