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Citations for Thermo Fisher :
8851 - 8900 of 10000+ citations for Recombinant Human IL18 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... Cells were scraped in PBS and a small aliquot was used to measure total protein using a Coomassie protein assay (Thermo Scientific, #1856209). Cells were lysed in cell extract buffer (100 mM Tris ...
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bioRxiv - Microbiology 2019Quote: ... The protein purity was assessed on SDS-PAGE with colloidal coomassie staining45 and protein yield was quantified by BCA-assay (ThermoFisher Scientific, USA). Imidazole and salts were removed by three washing steps with 21 ml dialysis buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2019Quote: ... The protein concentration of the clear supernatant was determined using the bicinchoninic acid assay (BCA Protein Assay Kit by Thermo Scientific Pierce).
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations of the tissue homogenates or whole-cell extracts were determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... The 3 eluted fractions were pooled and the protein concentrations measured with the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, 23225) using the Pierce™ Bovine Gamma Globulin Standard (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... CLICK-IT enrichment of AHA-labelled secreted proteins was performed with the Click-iT™ protein enrichment kit (Thermo Fisher Scientific, C10416) as previously described67,68 ...
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bioRxiv - Microbiology 2020Quote: ... Pre-cleared lysates were further incubated at 4°C overnight with the indicated antibodies (1 to 3 μl) and protein A agarose or protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitates were washed three times with RIPA buffer (LSB ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The protein content of supernatants was measured using a BiCinchoninic acid Assay (BCA) protein assay kit (Thremo Fisher Scientific, Mississauga, ON, Canada). Equal amounts of protein in lysis buffer (10 µg ...
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bioRxiv - Neuroscience 2021Quote: ... An input sample of 75 μl was removed from the lysate and protein concentration was assessed using a Pierce Bicinchoninic acid (BCA) protein assay kit (Thermo Scientific, 10678484). Concomitantly ...
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bioRxiv - Developmental Biology 2019Quote: ... Total protein was extracted from the homogenized whole kidney lysates using the M-PER mammalian protein extraction reagent (Thermo Fisher Scientific, 78501) supplemented with protease (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... 3.75% w/v stacking gel) prepared as described elsewhere [72] and run alongside a protein standard (NativeMark Unstained Protein Standard, Thermo Fisher Scientific) at 25 mA for 3 hr ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatant was placed in a new tube and the protein concentration was determined using Pierce BCA Protein Assay (Thermo Scientific 23227). Each reaction contained 15μg protein in a final volume of 230μl Proteasome buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were diluted to 2% (w/v) SDS and protein concentrations were determined using the Pierce BCA Protein Assay (Thermo Scientific, #23225). 20 μg of each lysate were subjected to an in-solution tryptic digest using a modified version of the Single-Pot Solid-Phase-enhanced Sample Preparation (SP3 ...
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bioRxiv - Physiology 2020Quote: ... followed by a 4-h incubation in Protein A magnetic beads (Pierce™ Protein A Magnetic Beads, #88845, ThermoFisher Scientific, Waltham, USA) and isolated using a magnetic rack ...
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bioRxiv - Physiology 2021Quote: ... The total concentration of proteins was measured with the Pierce Rapid Gold BCA Protein assay kit (Thermo Fisher Scientific, Rockford, IL, USA). The E- ...
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bioRxiv - Cell Biology 2021Quote: ... and an equal protein mass of 30ug in 10μL was subjected to NuPAGE™ 4-12 % Bis-Tris Midi Protein Gels (Invitrogen™, NP0335BOX) in Xcell4 Surelock tank (Life Technology SAS ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mM MgCl2) for cell membrane binding proteins (LPL, Caveolin 1) and quantified with Pierce™ BCA Protein Assay Kit (Buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were lysed in ddH2O by freeze/thaw cycles and the protein concentration was determined using the Pierce™ BCA protein assay kit (ThermoFisher Scientific). Polyamines were extracted by Bligh and Dyer extraction (Noga et al. ...
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bioRxiv - Plant Biology 2020Quote: ... Samples were resuspended in 2x Laemmli buffer and quantified for protein concentration using the Pierce 660nm Protein Assay Reagent (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2020Quote: A total of 85 μg of protein per sample was subjected to one-dimensional SDS-PAGE using an 8-16% precise protein gradient gel (Thermo Fisher Scientific). Proteins were separated for 1 cm in the resolving gel and the entire lane was excised and cut into equal pieces ...
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bioRxiv - Immunology 2020Quote: ... or with two administrations two weeks apart of 2.5μg Spike protein (2019-nCoV Spike Protein-S1+S2 ECD, His tag, SinoBiological) formulated in final 20mg/ml Imject™ Alum Adjuvant (Thermo Scientific) via bilateral intramuscular injections in the quadriceps (50μl per side) ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were clarified by centrifugation (12,000 g for 30 min at 4 °C) and protein concentrations were determined using a BCA protein assay kit (Thermo Fisher Scientific). SOD activity in whole cell extracts was measured using the EnzyChrome superoxide dismutase assay kit (BioAssay Systems ...
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bioRxiv - Neuroscience 2020Quote: ... ATP quantities were finally referred to protein amount using the Bradford assay (Coomassie Plus™ Protein Assay Reagent, Thermo Scientific, Schwerte, Germany).
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bioRxiv - Cell Biology 2020Quote: ... Cell debris were removed by centrifugation at 1000 × g for 5 min at 4°C and the protein concentration of the resulting supernatant was estimated using the BCA protein assay kit (Thermo Fisher Scientific) with bovine serum albumin as the standard ...
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bioRxiv - Cell Biology 2021Quote: ... Guanidine lysis buffer (above) was added to frozen pellets for sonication and protein concentration was assessed using BCA Protein Assay Reagent (Thermo Scientific, v23225). Lysates were equalized for protein concentration and incubated with NiNTA agarose beads (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... incubated for 5 min at 95 °C and 80 μg of protein was separated on a 10% SDS-PAGE gel (alongside PageRuller™ Plus Prestained protein Ladder, Thermo Scientific). Samples were transferred onto a nitrocellulose membrane (Amersham Protan 0.45 μm ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were cut in around the 55 kDa marker band of the protein marker (PageRuler Prestained Protein Ladder, Thermo Fisher Scientific). The upper portion of the membrane was probed with anti-HA tag antibody (mouse ...
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bioRxiv - Microbiology 2021Quote: For analysis of the soluble protein fraction 50 µg protein were reduced with 2.5 mM TCEP (tris-(2-carboxyethyl) phosphine hydrochloride) (Invitrogen, Thermo Fisher Scientific) at 65 °C for 45 min before thiols were alkylated in 5 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... A loading control was performed by total protein staining using Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes (Thermo Fisher Scientific, 24580). For blocking ...
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bioRxiv - Cell Biology 2022Quote: ... Mitochondria enriched fractions were processed similar to whole cell samples except they were normalized via protein concentration as determined by the Pierce™ BCA Protein Assay kit (Thermo Scientific). In Brief ...
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bioRxiv - Cancer Biology 2022Quote: ... Li-COR chameleon duo pre-stained protein ladder (Li-COR, 928-60000) along with 100 μg of protein combined with loading buffer (Thermo Scientific, NP0007) were loaded onto a 4%-12% Bis-Tris gel (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The total protein levels in the samples were assayed using the Pierce™ BCA Protein Assay kit (Thermo Fisher Scientific; Cat. # 23227) and diluted with 500 mM TEAB so that the total protein level of each sample would be approximately the same ...
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bioRxiv - Developmental Biology 2022Quote: ... the protein concentration in the supernatant was measured using the Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific, Waltham, MA, USA). After diluting with loading buffer and boiling for 10 min at 95°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Insoluble material was discarded and soluble proteins were recovered and quantified by the Bio-Rad Protein Assay (Hercules, CA) and the Nanodrop Drop One Spectrophotometer (Thermo Fisher Scientific). Cytoplasmic and nuclear extracts were obtained using the NE-PER Nuclear and Cytoplasmic Extraction reagent kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... 4 or 5 ug of appropriate antibodies or IgG control proteins were bound to protein G Dynabeads™ as per manufacturer instructions (Life Technologies). Equal portions of cleared lysates were added to antibody or IgG bound Dynabeads™ and incubated for 3 hours or overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The total protein concentration for each culture was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). Using the same amount of protein from each sample ...
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bioRxiv - Immunology 2022Quote: ... His-tagged proteins were purified from yeast supernatants by Ni-NTA resin affinity chromatography and protein concentration was determined using Coomassie (Bradford) Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... 10 min at 4°C and supernatants quantified for protein content using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23227) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... Expression levels of proteins of interest were normalised by total protein amount (Ghosh et al, 2014) (Moritz, 2017) using a Pierce reversible protein stain kit (#24580, Thermo Fisher Scientific). Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150 ...
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bioRxiv - Physiology 2022Quote: GNM muscles of mice euthanized in the morning in fed state were ground in liquid nitrogen and proteins were extracted from the powder obtained using Mammalian protein extraction reagent (MPER, #78501, Thermo Fisher Scientific) supplemented with halt protease and halt phosphatase inhibitors (#78429 and #78426 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were estimated using the PierceTM BCA Protein Assay Kit (Cat# 23225, Pierce Biotechnology, Thermo Fisher Scientific Inc., Waltham, MA, USA). Proteins ...
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bioRxiv - Microbiology 2022Quote: ... Unlysed cells were removed by centrifugation at 15 700 rcf for 30 minutes at 4oC and the concentration of proteins within the clarified lysate was then measured using the Qubit Protein Assay (Thermofisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and a 1:10 dilution of 2 μl of the samples was used to measure protein concentration by Qubit Protein Assay (Invitrogen, Cat# Q33211). The remaining 10 μl were reduced ...
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bioRxiv - Plant Biology 2022Quote: ... the induction media was removed by centrifugation and proteins were extracted by the resuspension of bacteria in B-PER™ Bacterial Protein Extraction Reagent (ThermoFisher Scientific) with 0.8 U/mL DNase I (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... The homogenates were centrifuged at 4 °C for 30 min at 9300 × g and total protein concentration in the supernatants was determined using the Pierce™ BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Physiology 2023Quote: ... Total IL-1β protein level was measured by mouse IL-1β/IL-1F2 DuoSet ELISA kit and first normalized to total protein level quantified by Pierce™ BCA Protein Assay Kit (Thermo Scientific), and then normalized to the sham group.
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bioRxiv - Neuroscience 2022Quote: ... Peptide sequences (and hence protein identity) were determined by matching protein databases with the acquired fragmentation pattern by the SEQUEST software program (Thermo Fisher Scientific). The data were filtered to establish a false discovery rate between one and two percent by using a database of mouse protein sequences made up of half real protein sequences and half reversed sequences ...
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bioRxiv - Neuroscience 2022Quote: The proteomics was performed by the serum after removing common high-abundance protein through using a Thermo Fisher’s serum High Abundance protein removal reag ent (High Select™ Top14 Abundant Protein Depletion Mini Spin Columns, A36370, Thermo fisher, US). The mass spectrometer Thermo Scientific Q Exactive was perfor med for Label-free quantification detection and data was analyzed by Proteome Disco verer 2.2 ...
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bioRxiv - Molecular Biology 2022Quote: ... SDS at 100 volts for 70 min until the 14 kD protein marker in SeeBlue Plus2 pre-stained protein standard (Life Technologies, USA) reached the bottom end of the gel ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were cleared by centrifugation at 4°C and 23,000×g for 30 min and protein concentration determined using Pierce(tm) BCA Protein Assay (Thermo Fisher Scientific, USA). 0.2 μg/μl α-casein were added to a protein amount of 1.2 mg ...