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Citations for Thermo Fisher :
8851 - 8900 of 10000+ citations for PKC beta 2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The gland/crypt-containing supernatant was washed by centrifugation at 400 x g for 2 minutes and the glands/crypts re-suspended in 1-2 ml advanced DMEM/F12 (12634-010; Gibco) containing 1X B27 supplement minus vitamin A (12587-010 ...
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bioRxiv - Cancer Biology 2021Quote: ... were cultured in EBM-2 Basal Medium supplemented with EGM-2 MV Microvascular Endothelial Cell Growth Medium and 1% v/v Pen-Strep (ThermoFisher) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: Staining the cells with the mitochondrial selective dye Rhod-2 AM (rhodamine-2 acetoxymethyl ester; Thermo Fisher Scientific, Darmstadt, Germany), allows for specific evaluation of mitochondrial calcium accumulation ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 7.4) containing 2 mM glucose for 2 hr with 100 nM of tetramethylrhodamine methyl ester (TMRM; Invitrogen, CA, USA). After the incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μL (200 pmol) each of gRNA and tracRNA were combined with 2 μL of 5X annealing buffer (ThermoFisher 100061876) and 4 μL nuclease-free dH2O ...
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bioRxiv - Microbiology 2021Quote: ... coated with 200 ng/well ACE2 [produced in-house as described in Bosnjak et al.(25)] and blocked with 2% bovine serum albumin/2% mouse serum (Invitrogen) and incubated for additional 1 h at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg of hK14-LaNto31-T2A-mCherry or LaNt-α31-pSec-Tag and 2 μl Lipofectamine 2000 (Thermo Fisher Scientific) were used ...
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bioRxiv - Bioengineering 2022Quote: ... Porcine DFAT adipocytes were stained for lipids via a 15-30 minute incubation at 37°C in lipid accumulation media (sans Intralipid) supplemented with 2 μM BODIPY 493/503 and 2 μg/ml Hoescht 33342 (H3570; Invitrogen). DFAT cells were then fixed in 4% PFA at RT for 10-15 minutes ...
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bioRxiv - Microbiology 2022Quote: The monoclonal antibodies SARS-CoV-1/SARS-CoV-2 Spike Protein S2 (1A9) and SARS-CoV-1/SARS-CoV-2 Nucleocapsid (6H3) were purchased from ThermoFisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... 2 µg fresh bacmid DNA was transfected into 2 mL Sf9 cells at 0.9×106 cells/2 mL with 8 µL Cellfection II (Thermo Fisher) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1% Glutamax (35050-038)) which was exchanged for 2 ml feeding medium 2 hours after plating (Neurobasal medium (21103-049, Gibco), 2% B27 (17504-044 ...
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bioRxiv - Biophysics 2020Quote: ... The resulting peptic peptides were collected for three minutes on a trap column (2 mm x 2 cm) kept at 0.5 °C and filled with POROS 20 R2 material (Thermo Scientific). The trap column was then placed in line with an ACQUITY UPLC BEH C18 1.7 μm 1.0 x 100 mm column (Waters ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were then washed in PBST-2 (PBS containing 0.1% Triton X-100) for 3 x 2 mins and blocked with SeaBlock blocking buffer (Thermo Fisher) for 15 mins at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... NPCs were plated on Geltrex in a 6 well plate in StemPro NSC SFM supplemented with 20ng/ml of EGF and bFGF for 2 days before switching to astrocyte differentiation medium consisting of DMEM with 1% N-2 supplement (Gibco), 1% GlutaMAX-I supplement (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... was diluted 1:1 with PBS (GE lifescience) with the addition of 2% fetal bovine serum (GE lifescience) and 2 mM EDTA (Ambion), added to lymphoprep tubes (Alare Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... at a concentration of 2 × 105 cells ml−1 (2 ml per dish hereafter) in DMEM (Nissui Pharmaceutical) supplemented with 10% FBS (Gibco), glutamine ...
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Trade-offs between antiseptic cytotoxicity and efficacy in a human ex vivo wound contamination modelbioRxiv - Microbiology 2021Quote: ... each bisect was rinsed in PBS and placed in an individual well of a 6-well plate with 2 mL MTT solution (2 mg/mL, Invitrogen) in each well ...
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bioRxiv - Neuroscience 2022Quote: ... The following three days (day 0-2) medium was replaced daily with 2 ml/well fresh differentiation medium: DMEM/F12/N2/NEAA (Invitrogen), penicillin/streptomycin (1% ...
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bioRxiv - Developmental Biology 2022Quote: ... the cultures were fed every 2 days with RPMI containing 2% B27 supplement plus insulin (Life Technologies Australia, Cat.#17504001). Cells were dissociated on day 15 of differentiation using 0.5% EDTA ...
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bioRxiv - Biophysics 2022Quote: ... at a concentration of 2×105 cells/ml (2 mL per well hereafter) in DMEM (Nissui Pharmaceutical) supplemented with 10% (v/v) FBS (Gibco), glutamine ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM D-Glucose, 2 mM L-Glutamine, 1 mM Sodium Pyruvate, 140 mM KCl and 20 mM HEPES, Invitrogen) for longer imaging sessions ...
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bioRxiv - Immunology 2019Quote: Mouse spleens were forced through a 70 μm mesh into FACS buffer (PBS containing 2 % heat-inactivated FBS and 2 mM EDTA) and red blood cells were lysed in ACK lysing buffer (Gibco) for 3 min ...
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bioRxiv - Cancer Biology 2020Quote: ... 300,000 cells in 6-well plate were transfected for 48 h with 2 µg of plasmid DNA using Lipofectamine 2 000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2019Quote: ... We subsequently placed two frozen individuals from each family and treatment group (n = 9 families * 2 treatments (+/− supplemental thiamine) * 2 individuals = 36) into 10 volumes of RNAlater-ICE (Invitrogen) pre-chilled to −80 °C and allowed the samples to reach −20 °C overnight ...
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bioRxiv - Physiology 2020Quote: ... and incubated with 100 mM 6-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (6-NBDG) (Life Technologies) in 10 nM Tris/HEPES buffer containing 150 mM KCl or 150 mM NaCl for 30 minutes at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... washed with 2 % FCS/PBS and then re-suspended in 2 % FCS/PBS containing 7-amino actinomycin (7-AAD, Invitrogen) at a concentration of 5 µg/ml ...
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bioRxiv - Biochemistry 2021Quote: Four rounds of panning were used to isolate scFvs binding both MERS-CoV S2 and SARS-CoV-2 spike using the following solutions coated on high binding plates: 2 μg/mL anti-c-myc tag antibody (Invitrogen) to eliminate phage expressing no or truncated scFv (Round 1) ...
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bioRxiv - Plant Biology 2021Quote: ... protoplasts were resuspended in lysis buffer (45mM magnesium chloride, 30mM sodium citrate, 20mM MOPS, 0.5% triton, 2% 4’,6-diamidino-2-phenylindole (DAPI, Thermo Scientific), pH 7 ...
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bioRxiv - Genomics 2021Quote: ... NGS was performed using primer sets that tiled the entire SARS-CoV-2 genome (Ion AmpliSeq SARS-CoV-2 Research Panel, ThermoFisher), with library preparation and sequencing performed on Ion Chef and S5 ...
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bioRxiv - Immunology 2022Quote: ... Yeast was routinely grown in YPD broth (2% peptone [Fisher Scientific], 2% glucose [Fisher Scientific], and 1% yeast extract [Sigma]) at 30 °C for 24 hours at 200rpm ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: ... Slides were dehydrated by sequential submersion in 100% ethanol (2× 5 min) and SafeClear II (2× 5 min, Fisher Scientific) before being mounted in Cytoseal 60 (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... Yeast was routinely grown in YPD broth (2% peptone [Fisher Scientific], 2% glucose [Fisher Scientific], and 1% yeast extract [Sigma]) at 30 °C for 24 hours at 200rpm ...
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bioRxiv - Microbiology 2022Quote: ... and 2.5 µl were applied to a freshly glow-discharged Quantifoil R 2/2 Cu/Rh 200 mesh grid and plunge-frozen into liquid ethane using a Vitrobot Mark IV (ThermoFisher) at 100% humidity at 10 °C ...
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bioRxiv - Microbiology 2022Quote: ... 2.5 µl of the sample was applied to a freshly glow-discharged Quantifoil R 2/2 Cu/Rh 200 mesh grid and plunge-frozen into liquid ethane using a Vitrobot Mark IV (ThermoFisher) at 100% humidity at an ambient temperature of 10 °C ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells were infected with the SARS-CoV-2 wild type (isolate SARS-CoV-2/human/SWE/01/2020; Genbank accession: MT093571) in VP-SFM (Gibco). The virus was harvested at day three ...
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bioRxiv - Neuroscience 2023Quote: ... the plating medium was removed and replaced with maintenance media (neurobasal medium [Gibco, cat# 21103049] with 2% B27 [Gibco, cat# 17504044], 2 mM GlutaMAX [Gibco, cat# 35050061] ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was extracted from the thalamus of 2 GAERS (10 and 90 days) and 2 NEC (10 and 90 days) male rats using a MagMax Kit (Ambion) and full length cDNAs generated using SuperScriptII with oligo-dT priming (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting peptic peptides were collected on a trap column (2 mm x 2 cm; 0.5 °C) filled with POROS 20 R2 (ThermoFisher Scientific). After 3 minutes ...
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bioRxiv - Immunology 2024Quote: ... fraction in MACS buffer (2% heat-inactivated FBS, 2% 0,1M EDTA in PBSO) using the CD8+ Dynabead isolation kit (Thermo Fisher) and BD IMag Cell Separation Magnet (BD Biosciences) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cell populations were imaged in media at the designated time points at 10x magnification and 2×2 binning using the ArrayScanTM XTI High Content Reader (ThermoFisher). Tomato MFI and EGFP MFI was imaged with an excitation source of 560/25nm and 485/20nm respectively ...
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bioRxiv - Cell Biology 2023Quote: ... In brief pulse labeling with FM4-64 (2 μM FM464, Molecular Probes; made from a 2-mM stock in DMSO) was done for 5 min (time 0 ...
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bioRxiv - Neuroscience 2023Quote: ... particle size = 3 μm, C18, L = 2 cm; analytical column: particle size = 2 μm, C18, L = 50 cm; PepMap, Dionex/Thermofisher). Peptides eluting from the column were ionized using an Orbitrap Elite (Thermofisher).
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bioRxiv - Pathology 2023Quote: ... Peptides were eluted under a 60min 2%-37% acetonitrile gradient for mouse degradome (2%-45% for human degradome) and transferred by Nanospray Flex nanoelectrospray source (ThermoFisher) into an Orbitrap Elite - ETD mass spectrometer (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... MDA-MB-231 cells were plated as above and treated with 100 nM of Cy5-labeled si<(EG18L)2 or si<(EG18Ldiacid)2 in Opti-MEM (Gibco) for 2 h at 37°C vs ...
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bioRxiv - Bioengineering 2023Quote: ... The concentrated virus was diluted 1:5 in hepatocyte medium containing N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (HEPES; 20 mM; Gibco) and 4 μm/mL polybrene (Sigma ...
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bioRxiv - Synthetic Biology 2023Quote: ... in Tris-Glycine SDS Running Buffer (225V, 45 min)and transferred to nitrocellulose (iBlot 2, iBlot 2 NC Mini Stacks; Invitrogen) at 20 V for 1 min ...
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bioRxiv - Bioengineering 2023Quote: ... Cetuximab and Avelumab F(ab’)2 fragments were generated using the Pierce F(ab’)2 preparation kit (Thermo Fisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Primary T lymphocytes stimulated with PHA and IL-2 (2 × 105, 100 µl/well) were resuspended in HBSS buffer (Gibco) containing 0.1% (w/v ...