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Citations for Thermo Fisher :
8801 - 8850 of 10000+ citations for 6 THIOPHEN 2 YL 1H INDAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Real-time PCR reactions were run on a QuantStudio 6 Flex machine using PowerUp SYBR Green master mix (Applied Biosystems) using gene specific primer sets (Table 1) ...
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bioRxiv - Cell Biology 2022Quote: ... Myc-Arhgef18 and/or the septin complex (Flag-SEPTIN2, Flag-SEPTIN6, Flag-SEPTIN7, GFP-SEPTIN9) on 6-well tissue-culture plates using Lipofectamine 3000 (Invitrogen) and harvested 18–24 h later ...
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bioRxiv - Cell Biology 2022Quote: ... qPCR analysis was performed on a 384-well plate with the QuantStudio 6 Flex Real-Time PCR System (Life Technologies) using GeneAce SYBR qPCR Mix (Nippon Gene) ...
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bioRxiv - Microbiology 2022Quote: ... The qPCR amplification was performed on an Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Inc.). Thermocycler conditions consisted of 2 min incubation at 50 °C followed by 10 min incubation at 95 °C and 40 cycles at 95 °C for 15 s and 55 °C for 1 min followed by denaturation at 95□ for 15 sec and melt curve analysis from 60□ (1 min ...
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bioRxiv - Genetics 2022Quote: Cells plated in 10 cm Petri dishes were lysed and scrapped in 6 mL ice-cold TRIzol™ Reagent (Invitrogen) added directly to the culture dish after removal of the growth medium ...
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bioRxiv - Molecular Biology 2022Quote: ... cells seeded in a 6-well plate were transfected with 1 µg of pPRIME-FLuc plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... and the amplified DNA fragment and an annealed linker oligo (for GFP: primers 5 and 6) were introduced into the pENTR/D-TOPO vector (Invitrogen; sequences were confirmed by Sanger sequencing using primers 7 and 8 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed twice in FACS buffer and analyzed by flow cytometry on an Attune NxT 6 cytometer (ThermoFisher). Antibodies used are shown in Supplemental Methods ...
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bioRxiv - Immunology 2022Quote: ... bone marrow progenitors were collected from the femurs of adult C57BL/6 WT mice and 10 × 106 progenitor cells were cultured in RPMI media (Gibco) supplemented with 10% FBS (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... For glutamine starvation experiments cells were washed once with PBS and incubated for 6 hours in DMEM/F12 without glutamine and supplemented with 10% (v/v) dialyzed FBS (Gibco), 1% (v/v ...
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bioRxiv - Cell Biology 2022Quote: HeLa mito-mKeima cells were seeded at 200,000/well in a 6-well plate 12-16h prior to transient transfection with the indicated siRNAs (20 nM) with Lipofectamine RNAiMAX (ThermoFisher). The liposome/siRNA mixture was added to culture media for 24h ...
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bioRxiv - Cell Biology 2022Quote: ... dissociated neurons were resuspended and cultured at 37°C in a humidified incubator with 5% CO2 and 95% O2 in poly-D-lysine coated 6-well plates in neurobasal media (12349015, Gibco) supplemented with 1% GlutaMAX™ Supplement (35050-061 ...
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bioRxiv - Cell Biology 2022Quote: ... at a concentration of 350,000 cells/ml and were cultured in neuromesodermal progenitor cell induction medium (NMP media) containing Essential 6 media (Gibco, A1516401), 1.5 mM CHIR (Tocris biotech ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from ∼100 mg of flash-frozen hairy root tissue and qRT-PCR reactions were performed using a QuantStudioTM 6 (ThermoFisher) as previously described 19 ...
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bioRxiv - Molecular Biology 2020Quote: ... Trypsinized hippocampi were washed two times with the same HBSS buffer before being triturated 5-6 times with a fire-polished glass Pasteur pipette in BME (Invitrogen) supplemented with 10 percent FBS (VWR) ...
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bioRxiv - Molecular Biology 2020Quote: Targeting was carried out by transfecting 3 μg of each Cas9 guide and 10 μg of targeting construct into ESCs in one well of a 6-well plate using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Targeting was carried out by transfecting 3 μg of each Cas9 guide and 10 μg of targeting construct into ESCs in one well of a 6-well plate using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... ESCs in one well of a 6-well plate were transfected with 10 μg of expression vector using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Human Drosha cDNA with a Flag-tag at the amino-terminus and a 6 x his tag at the carboxyl-terminus was cloned into pcDNA4/TO (Invitrogen) to construct the inducible WT Drosha expressing plasmid for immunoprecipitation assay and ubiquitination assay ...
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bioRxiv - Genomics 2020Quote: ... We then amplified the library using Hi-fidelity KAPA PCR kit (KAPA) for 6 cycles and quantified the library using Qubit high sensitivity DNA assay (Thermofisher). We performed QC using bioanalyzer (Agilent ...
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bioRxiv - Genomics 2020Quote: ... We then amplified the library using Hi-fidelity KAPA PCR kit (KAPA) for 6 cycles and quantified the library using Qubit high sensitivity DNA assay (Thermofisher). We performed QC using bioanalyzer (Agilent ...
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bioRxiv - Immunology 2020Quote: ... containing the gp67 secretion signal peptide and a C-terminal 6×His tag was inserted into pFastBac-Dual vectors (Invitrogen) and transformed into DH10Bac component cells ...
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bioRxiv - Cell Biology 2021Quote: 1×106 L1UP and Ctrl mESCs were seeded in 10 cm dish 16 hours prior to transfection with 6 μg of JJ-L1SM (WT and L1N21A) plasmid using Lipofectamine 3000 (Invitrogen). Media exchange was initiated 6 hours post transfection and hygromycin supplemented media was added 48 hours post transfection to select for stably transfected cells ...
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Highly efficient homology-directed repair using transient CRISPR/Cpf1-geminiviral replicon in tomatobioRxiv - Molecular Biology 2019Quote: ... 2015) (Supplemental Table 6 and 7) junctions and a high-fidelity Taq DNA polymerase (Phusion Taq, Thermo Fisher Scientific, USA) and Sanger sequencing (Solgent ...
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bioRxiv - Neuroscience 2020Quote: ... Roughly 100,000 HPCs per well were seeded into a 6-well plate in custom made media containing DMEM/F12 (ThermoFisher, 11320033), 2X insulin transferrin selenite (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 μg of pcDNA3.1+ empty vector were transfected into cells at least 6 hours post-siRNA transfection using lipofectamine LTX with Plus reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... ARPE-19 cells were transfected by reverse transfection in 24-well tissue culture plates as follows: A total of 6 pmols of siRNA was diluted in 100 μl of OptiMem (Gibco) per well ...
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bioRxiv - Neuroscience 2020Quote: ... the hippocampi of embryonic day 17 C57BL/6 mice (Koatech, South Korea) were dissected and digested with 0.125% Trypsin-EDTA solution (Gibco, USA) for 15 min in a 37 °C water bath ...
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bioRxiv - Microbiology 2021Quote: Vero E6 cells (ATCC CDL-1586) were grown to 80% confluence in 6-well tissue culture plates in DMEM (ThermoFisher Scientific/Gibco) containing 5% FBS (ThermoFisher Scientific/Gibco ...
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bioRxiv - Synthetic Biology 2022Quote: ... The optical density of the cells was monitored by the UV-Vis spectrophotometer (Genesys 6, Thermo Fisher Scientific, Waltham, WA) (Figure S2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Embryos were then washed in fresh E3 medium and kept in groups of 6 in 24-well polystyrene plates (Nunc) in 1 ml of E3 water ...
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bioRxiv - Immunology 2022Quote: ... which had been placed in the wells of a 6-well plate filled with 1.1 ml phenol red-free RPMI medium (Thermo Fisher) supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... were mixed with 20 ng of genomic DNA and genotyped using a Quantstudio 6 Flex System according to the manufacturer’s instruction (Applied Biosystems).
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from rSARS-CoV-2/mCherry-Nluc-infected (MOI 0.01) Vero E6 cells (1.2 x 106 cells/well, 6-well format) using TRIzol reagent (Thermo Fisher Scientific) based on the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: 1×105 N2A or HEK293T (ATCC) cells were seeded in one well of a 6-well plate in 2mL of DMEM complete media (Gibco) supplemented with 10% Fetal Bovine Serum ...
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bioRxiv - Microbiology 2022Quote: Transduced BJ-hTERT cells were seeded in 6-well plates or 8-well chamber slides (Nunc™ Lab-Tek™). Cells were fixed in 4% paraformaldehyde in PBS (Alfa Aesar ...
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bioRxiv - Genomics 2022Quote: ... the assay was carried out in 6-well plates coated with a bottom layer of 1% noble agar in 2X DMEM (ThermoFisher) supplemented with ...
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bioRxiv - Molecular Biology 2019Quote: ... one cDNA fraction corresponding to 70–200 nt was gel purified after 6% TBE-urea gel electrophoresis (Thermo Fisher Scientific). After the final PCR step ...
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bioRxiv - Immunology 2019Quote: ... Spleens from 12-52 week old C57BL/6 mice were mashed through a 40-μm cell strainer treated with ACK lysis buffer (1 mL per spleen, ThermoFisher) for 5 min at 25°C to lyse red blood cells ...
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bioRxiv - Cell Biology 2019Quote: ... sample aliquots containing 25 µg of dried peptides were subsequently labeled with isobaric tag (TMT 6-plex, Thermo Fisher Scientific) following a recently established protocol 45 ...
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bioRxiv - Biophysics 2020Quote: ... The samples were then allowed to adsorb on the grid for 30s before blotting away the excess buffer using a blot force of 6 and blot time of 6s in a FEI Vitrobot Mark IV (ThermoFisher). The grids were plunge frozen in a liquid ethane slush and stored in liquid nitrogen until imaging ...
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bioRxiv - Microbiology 2020Quote: ... 6-well plate format) infected at multiplicity of infection (MOI) of 0.01 were extracted using TRIzol reagent (Thermo Fisher Scientific), according to the manufacturer’s specifications ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR was conducted using a 5’ fluorescently-labelled primer (6-FAM, PET, NED, or VIC) (Applied Biosystems, Foster City, CA) and an unlabeled reverse primer ...
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bioRxiv - Cell Biology 2019Quote: ... Two x 106 cells/well of a 6-well plate were seeded in DMEM containing 10% FCS (Invitrogen, Carlsbad; USA), 1% HEPES (Carl Roth ...
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bioRxiv - Biochemistry 2020Quote: The C-terminal fragments of Stu2 used in the pulldown experiments were cloned into a pLIC vectors with a TEV-cleavable N-terminal 6-His tag and transformed into Bl-21 AI cells (Invitrogen). Cells were grown to an OD600 of 0.6 in TB media with 0.1% glucose and were induced by addition of IPTG and arabinose to 200 μM and 0.2% ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293t were replated every 3 days at a split ratio of 1 in 6 following dissociation with Trypsin 0.05% EDTA (Gibco, Life technologies).
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bioRxiv - Genetics 2021Quote: ... the PCR products from either primer pairs 6/7 or 9/10 with the plasmid vector pCR 2.1 (Thermo Fisher) followed by transformation of One Shot Top 10 chemically competent cells (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were seeded onto 6-well cell culture plates and transiently transfected with different GIPR constructs using Lipofectamine 2000 transfection reagent (Invitrogen). All the mutant constructs were modified by single-point mutation in the setting of the WT construct (HA-Flag-3GSA-GIPR(22-466)) ...
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bioRxiv - Cell Biology 2021Quote: ... They were washed in PBST for 6 × 10 min before being incubated with secondary antibody (1:400 in PBST) (ThermoFisher) for 30 min at room temperature ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... HUVEC seeded at 1.5 × 106 cells per well of a collagen-coated 6-well pate were transfected with plasmids using the Neon Transfection System (Invitrogen), harvested 48 hours post-transfection ...