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Citations for Thermo Fisher :
8751 - 8800 of 10000+ citations for Recombinant Human TNFRSF21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... anti-human CD19 (HIB19) and IgM (SA-DA4) antibodies were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2023Quote: Human ileocecal adenocarcinoma (HCT-8) cells (ATCC) were cultured in RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: Human JAGGED1-Fc (Martin et al., 2023) was transfected into Expi293F cells (ThermoFisher, A14527) using FectroPro (Polyplus ...
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Hallmark molecular and pathological features of POLG disease are recapitulated in cerebral organoidsbioRxiv - Cell Biology 2023Quote: Each human cerebral organoid was fixed in 4% paraformaldehyde (PFA, Thermo Fisher Scientific, #28908) in PBS overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: Human Flvcr2 was knocked down in HBEC5i cells using siRNA (Silencer Select siRNA; Ambion). Cells were transfected with either scrambled siRNA or Flvcr2 siRNA using Lipofectamine 3000 (Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... human fibroblasts were resuspended by trypsinisation (Gibco™Trypsin-EDTA (0.25%) (Thermo Fisher Scientific)) and then centrifuged at 500 × g for 5 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... Human iPSCs were cultured in feeder-free conditions on GelTrex-coated plates (ThermoFisher Scientific) in mTeSR-1 media (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2024Quote: The human DBT cDNA plasmid was obtained from the Ultimate ORF collection (Thermo Fisher). Synonymous mutations in the DBT cDNA were generated with Q5-site direct mutagenesis (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor 488- and Alexa Fluor 594-conjugated human transferrin were obtained from Invitrogen. Brefeldin A (BFA) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were quantified by an ELISA kit for total human tau (ThermoFisher Cat# KHB0041) as per manufacturer’s protocol to determine brain ISF tau levels before ...
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bioRxiv - Molecular Biology 2024Quote: ... RRID:CVCL_0291) and Flp-In-T-REx-293 (human, female, embryonic kidney, epithelial; Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... were cultured as standard in Human Large Vessel Endothelial Cell Basal Medium (Gibco, M200500) supplemented with low serum growth supplement (Gibco ...
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bioRxiv - Immunology 2024Quote: ... Isolated T cells were immediately activated with DynaBeads Human T-Activator CD3/CD28 (ThermoFisher), using a 1:1 bead:cell ratio ...
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bioRxiv - Systems Biology 2024Quote: ... The resulting suspension of human tumor cells was adjusted in DMEM medium (Gibco, #11965084) to a concentration appropriate for loading into a 10X genomics Chromium controller (3’ gene expression kit ...
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bioRxiv - Physiology 2024Quote: ... and C3 (rabbit anti human, 1:100, cat. no.: PA5-21349, Thermo Fisher Scientific). Thereafter ...
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bioRxiv - Microbiology 2024Quote: ... Human foreskin fibroblast (HFF-1) cells (ATCC SCRC-1041) were maintained in DMEM (Gibco) with 20% FBS ...
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bioRxiv - Microbiology 2024Quote: Human Foreskin Fibroblasts (HFFs) were maintained in Dulbecco’s minimal essential medium (DMEM; Fisher Scientific) and BS-C-1 cells (ATCC CCL-26 ...
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bioRxiv - Developmental Biology 2024Quote: ... KLF2 was evaluated in human cells with the probe Hs07291763_gH (ThermoFisher Scientific, Cat.#: 4331182) and in zebrafish with klf2a ...
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bioRxiv - Physiology 2024Quote: ... Human THP-1 monocytes were cultured in RPMI 1640 medium (Gibco, cat no. 11875093) supplemented with 10% FCS ...
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bioRxiv - Immunology 2024Quote: ... an Alexa Fluor 647-labeled goat anti-human IgG [H+L] secondary antibody (Invitrogen) was used to detect bound IgG1 to the bacterial surface ...
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bioRxiv - Cancer Biology 2021Quote: ... Final sEVs pellet was resuspended in 100 μL of PBS and the protein content was measured by bicinchoninic acid assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific). The NS500 nanoparticle characterization system (NanoSight ...
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bioRxiv - Cell Biology 2020Quote: ... The resultant mixture was centrifuged at 14,000 g and the supernatant was used to estimate the total protein by using Pierce™ BCA Protein Assay Kit (Thermo scientific). Equal quantity of protein (300 μg ...
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bioRxiv - Cell Biology 2020Quote: ... sonicated 3 three times for 5 sec each and analysed for protein content using the BCA protein assay kit (Thermo Fisher scientific). Samples containing 30 μl (1 μg/μl ...
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bioRxiv - Cell Biology 2020Quote: ... Membrane pellets were gently resuspended in 100 μl SEM and protein content was measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) to normalize total protein concentration across samples ...
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bioRxiv - Cell Biology 2020Quote: ... The protein content of preovulatory follicles from eCG-injected mice is ~6 μg (Pierce BCA protein assay kit, 23225, Thermo Fisher Scientific). The protein content of follicles (~320-400 μm diameter ...
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bioRxiv - Cell Biology 2019Quote: ... anti-TAC102 antibody is crosslinked to protein A / protein G beads from the Pierce™ Crosslink Magnetic IP kit (Thermo Scientific™). A mitochondrial enriched fraction is prepared by digitonin fractionation with 100 ml of procyclic form parasites (cell line ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations in the supernatants were measured using the bicinchoninic acid assay (Pierce BCA Protein Assay Reagent, Thermo Scientific, Waltham, MA, USA). Equal amount of protein lysates (40 mg ...
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bioRxiv - Cell Biology 2020Quote: ... TAG amounts were normalized to the total protein amounts in each sample using a Pierce TM BCA Protein Assay Kit (Thermo Fisher Scientific). The data were analyzed using a Student’s t test.
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bioRxiv - Microbiology 2020Quote: The proteins from cell lysates and isolated mitochondria were analysed by separating 10-40 μg of protein (concentrations determined using Pierce BCA Protein Assay kit, Thermo Fisher Scientific), by SDS-PAGE ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were stored at - 80°C and protein concentrations were further determined using the Pierce™ BCA Protein Assay Kit (23225, ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were separated by SDS-PAGE in Bio-Rad mini-protean® Tetra system using PageRuler prestained protein ladder (#26616, Thermo scientific) as molecular-mass standards ...
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bioRxiv - Molecular Biology 2021Quote: ... the P2 fraction was brought up in 400uL of PBS and protein concentrations were measured using the QuBit Protein Assay Kit (Thermo Fisher, Q33211). Samples were precleared with 15mg of DEPC treated (to inactivate RNAse ...
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bioRxiv - Molecular Biology 2020Quote: ... PIWIL1 and PIWIL3 proteins were immunopurified using anti-MARWI (1A5) and anti-Hamster PIWIL3 (3E12) immobilized on Dynabeads protein G (Life Technologies, 10004D) and anti-Mouse IgG (IBL ...
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bioRxiv - Molecular Biology 2021Quote: ... 12 µg of protein per sample was used for gel electrophoresis with a NuPAGE™ 4-12% Mini Protein Gel (Invitrogen, NP0323) in a Mini Gel Tank (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature for all target proteins or with clear milk blocking buffer for β-actin protein (Pierce, Thermo Scientific). We used the following LARP1 (Bethyl Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein concentration of the isolated exosomes was detected by using a total exosome RNA and Protein Isolation Kit (Thermo Fisher Scientific) and a BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... SOD2-V5 protein in 125 or 250 μg lysate was immunoprecipitated with anti-V5 antibody and Dynabeads Protein G (Thermo Fisher Scientific), and then immunoblotted with 25 μg input samples.
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... The molecular migration position of transferred proteins was compared to the PageRuler Prestain Protein Ladder 10 to 170 kDa (Thermo Fisher Scientific). Signal quantification was performed using Image J software (NIH).
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bioRxiv - Neuroscience 2019Quote: ... The signal was normalized to the amount of protein in the sample as determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer instructions.
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bioRxiv - Microbiology 2019Quote: ... Samples were centrifuged at 10,000 x g for 30 min at 4 °C before supernatants were collected and protein content was determined using the BCA Protein Assay Kit (Pierce, Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: ... to remove RNA and DNA and then incubated overnight with the appropriate antibody and then incubated overnight with a 50:50 mixture of protein A and protein G Dynabeads (Thermo Fisher Scientific). Beads were washed three times with nondenaturing lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Genetics 2019Quote: ... The molecular migration position of transferred proteins was compared to the PageRuler Prestain Protein Ladder 10 to 170 kDa (Thermo Fisher Scientific). Signals were detected using the Chemidoc XRS+ imaging system (Biorad ...
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bioRxiv - Cancer Biology 2019Quote: ... we transferred the supernatant to a clean tube and determine the protein concentration using the EZQ® Protein Quantitation Kit (Cat. no. R33200,Thermo Scientific) or another method ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: We quantified the protein content in the clarified lysates of the damselflies using Pierce™ BCA protein assay kit (Thermo Fisher Scientific). We used the Bovine Serum Albumin (BSA ...
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bioRxiv - Physiology 2020Quote: ... and MOTS-c in each group was normalized to the total tissue protein concentration as determined by the Bradford protein assay (Thermo Fisher Scientific).
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bioRxiv - Physiology 2020Quote: ... Protein pellets from each aliquot per sample were pooled and the total protein content were measured using BCA protein Assay kit (ThermoFisher Scientific, Australia). The protein disulphide bonds were reduced with 10 mM dithiothreitol for 30 min at 60 °C and the cysteine residues were alkylated with 20 mM iodoacetamide for 30 min at 37 °C in the dark ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was centrifuged at (21000 g) for 15 min at 4°C and protein concentration determined (Pierce™ 660nm Protein Assay Reagent, Thermo Scientific). 8.5 μg lysate was kept for western blot analysis and 1.6-1.8 mg total protein was added to 250 μl washed high capacity neutravidin beads (Life Technologies ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Protein content in each sample was measured with NanoOrange Protein Quantification Kits according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA), and was used for ATP synthesis rate normalization ...
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bioRxiv - Biophysics 2019Quote: ... the final dye to protein ratio was typically 5-6 dye molecules per protein molecule as measured with a Nanodrop spectrophotometer (Thermo Fisher Scientific). Cells in suspension culture dishes were labeled with 0.5 nM fluorescent IgE for typically 12-20 hours before imaging.
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bioRxiv - Bioengineering 2019Quote: Proteins were collected 48 hours post-transfection and their concentrations were measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Western blots were performed as described previously (Lee et al ...