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Citations for Thermo Fisher :
8601 - 8650 of 10000+ citations for QuantiChrom Indole Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Epigenetics of post-operative delirium: A genome-wide DNA methylation study of neurosurgery patientsbioRxiv - Neuroscience 2022Quote: ... DNA quality was assessed with NanoDrop spectrometry and quantified with the Qubit dsDNA Broad Range Assay Kit (Q32850, ThermoFisher Scientific, Waltham, MA, USA). For each sample ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed with 15 μl RIPA buffer and protein concentration was quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). OCR measurements were normalized to the protein concentration in each well.
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bioRxiv - Cancer Biology 2022Quote: ... and the protein content was determined using the colorimetric micro–BCA Protein Assay Kit according to the instructions supplied by the manufacturer (ThermoFisher Scientific, Rockford, IL). The AssayMAP Bravo platform (Agilent Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... The concentrations of the nuclear extracts were measured with the Pierce™ BCA Protein Assay Kit Reducing Agent Compatible (Thermo Scientific™, 23250). 10 µg of nuclear extract were used for the TP53 functional assay that was conducted according to the manufacturer’s instructions with the calorimetric p53 Transcription Factor Assay Kit (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then fixed by adding 100 μL of Click-iT® fixative (Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit, ThermoFisher) and stored at 4°C for 24 hours ...
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bioRxiv - Bioengineering 2022Quote: Quantity of encapsulated mRNA and encapsulation efficiency were measured with the Quant-it™ RiboGreen RNA Assay Kit (cat# R11490; Invitrogen, Waltham, MA) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Edu incorporation and staining with the Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific, C10634) was performed according to the manufacturer’s instructions with the use of the fluorescent dye from Click-iT™ EdU Cell Proliferation Kit for Imaging ...
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bioRxiv - Genomics 2022Quote: ... DNA ligation products were resuspended in 130μl of nuclease free water and concentration was assessed by fluorimetric quantification using the Qubit dsDNA HS Assay Kit (Thermo Fisher cat. #Q32851).
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bioRxiv - Genomics 2022Quote: ... for 30 minutes at 4°C and supernatant was retained and protein concentration quantified using the Pierce BCA protein assay kit (Thermo Fisher Scientific, 23227). A total of 25-35μg proteins were loaded onto 12.5% Tris-HCl gel for SDS-PAGE analysis ...
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bioRxiv - Immunology 2022Quote: ... the cells were click labelled intracellularly using the Click-iT® EdU flow cytometry assay kit (#C10418, Invitrogen, Life Technologies Corporation, OR, USA) according to manufacturer instructions ...
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bioRxiv - Genomics 2022Quote: ... The final library was eluted with 13 µl elution buffer (EB) for 20 min at 48°C and DNA concentration was quantified using the Qubit dsDNA BR assay kit (Thermo Fisher Scientific, Q32850). A total of 400 ng was carefully mixed with 37,5 µl sequencing buffer (SQB) ...
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bioRxiv - Microbiology 2022Quote: ... Protein concentration of NAD+ or NADH extracts was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, cat. no. 23225, Rockford, IL) to normalize NAD(P)H and NAD(P)+ concentrations.
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bioRxiv - Microbiology 2022Quote: ... Firefly luciferase activity was measured in the cell lysates after removing the supernatant and washing the cells with warm PBS using the Pierce Firefly luciferase glow assay kit (Thermo Fisher Scientific # 16176). Luminescence was measured in Cytation3 multi-mode plate reader ...
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bioRxiv - Microbiology 2022Quote: ... Sections from the AstV-ND-1 patient’s brain and a control human brain were mounted onto microscope slides and hybridized with either astrovirus or actin probes using a QuantiGene (R superscript circularized) ViewRNA ISH Tissue 2-Plex Assay kit (Affymetrix Cat No. QVT0012).
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bioRxiv - Molecular Biology 2022Quote: ... Quantification of purified PCR products was determined by Qubit 4.0 Fluorometer using the 1X dsDNA HS Assay Kit (Thermo Fisher Scientific, MA, USA). Purified PCR products were mixed into equimolar concentrations (100 pmol ...
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bioRxiv - Microbiology 2022Quote: ... The RNA concentration was determined using NanoDrop Spectrophotometer ND-1000 or QubitTM HS RNA Assay kit (Invitrogen Thermo Fisher Scientific, Waltham, MA, USA). The quality of the bacterial RNA samples was examined based on the ratio of 23S/16S rRNA separated through agarose-electrophoresis (Farnsworth et al. ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Acetylcholinesterase activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction and normalized by protein concentration as determined by a Coomassie (Bradford) protein assay kit (Thermo Scientific, Waltham, MA). Activity was compared to the solvent control samples (set at 100% activity) ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was generated using the Ovation RNA-Seq System V2 (NuGen Technologies, 7102-A01) and quantified using the dsDNA Qubit HS Assay kit (Thermo Fisher Scientific, Q32854). This was followed by acoustic shearing of the cDNA to generate 200 bp fragments using Covaris E220 (Covaris) ...
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bioRxiv - Molecular Biology 2023Quote: ... The whole cell lysate were obtained with RIPA and the protein concentration was measured using BCA protein assay kit (23225, Thermo Scientific, MA, USA). Monoclonal antibody anti-DNMT1 (ab188453 ...
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bioRxiv - Molecular Biology 2023Quote: ... and Click-iTTM chemistry was conducted following the manufacturers’ instructions for the Click-iTTM HPG Alexa FluorTM 488 Protein Synthesis Assay Kit (Thermo Fisher Scientific, C10428). Nuclei were stained with DAPI ...
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bioRxiv - Plant Biology 2023Quote: ... and whole genomic DNAs were quantified using the Qubit dsDNA BR Assay Kit and Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, United States). Samples with high-molecular-weight DNA (>15 kb ...
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bioRxiv - Genomics 2022Quote: ... followed by quantification of double stranded DNA product using Quantit dsDNA Assay Kit Broad range and Nanodrop (Thermo Fisher Scientific, Waltham, MA, USA). Host DNA was sequenced using Illumina MiSeq at the Earlham Institute ...
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bioRxiv - Microbiology 2022Quote: ... pH 8) was added on each pellet and total protein quantification was performed using BCA Protein Assay kit (Pierce™, Thermo Fisher Scientific). Before immunobloting analysis ...
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bioRxiv - Neuroscience 2023Quote: The amount of LDH in the cell lysates and media was measured using CyQUANT LDH Cytotoxicity Assay kit (Thermo Fisher Scientific, Waltham, MA). The percentage of cell death was calculated by the total amount of LDH in the media divided by that in the cell lysates.
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bioRxiv - Molecular Biology 2022Quote: ... The isolated DNA was quantified by fluorometry using the Quant-iT high sensitivity dsDNA assay kit on the Qubit fluorometer (Invitrogen; Thermo Fisher Scientific). The DNA samples were stored at -20 °C until further molecular analyses.
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bioRxiv - Microbiology 2022Quote: ... The final purified DNA was stored at -80ºC and the DNA concentration was determined using Qubit HS Assay Kit on the Qubit 4 Fluorometric Quantification device (Invitrogen, Carlsbad, California, USA). The bacterial community composition was determined by Illumina NextSeq-based high-throughput sequencing (HTS ...
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bioRxiv - Microbiology 2022Quote: Total protein concentration in the crude cell lysates was measured using the Pierce™ BCA Protein Assay Kit (Thermo Scientific, Cat. No. 23225). The lysates were loaded in the amount of 5 µg of total protein in Laemmli sample buffer onto a 6% (v/v ...
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bioRxiv - Plant Biology 2022Quote: ... The library concentration was determined in a Qubit3 Fluorometer using the Qubit dsDNA HS (High Sensitivity) Assay Kit (Thermo Fisher Scientific, Waltham, USA). The library quality was assessed using Agilent D5000 ScreenTape System in a 4150 TapeStation System ...
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bioRxiv - Cell Biology 2022Quote: ... for miR-375 was quantified by TaqMan Assays using the TaqMan MicroRNA Reverse Transcription Kit and hsa-miR-375 primer sets (Thermo Fisher Scientific, 000564). MiR-375 levels were normalized to miR-U6 expression ...
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bioRxiv - Systems Biology 2024Quote: ... The detection of protein synthesis was performed according to the protocol provided by the Click-iT® Plus OPP Protein Synthesis Assay Kits (Thermo Fisher, C10456). In brief ...
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bioRxiv - Bioengineering 2024Quote: ... by washing in phosphate buffer saline (PBS) and storing films at −80 °C for DNA quantification using Quant-iT™ PicoGreen™ dsDNA Assay Kit (ThermoFisher Scientific), per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of extracted DNA was determined fluorometrically using Qubit® DNA HS (High Sensitivity) Assay Kits (Thermo Scientific™, Waltham, MA, USA) on a Qubit® 4 fluorometer (Thermo Scientific™ ...
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bioRxiv - Microbiology 2023Quote: ... DNA was eluted into 10mM Tris and quantified using the Qubit™ 1X dsDNA High Sensitivity (HS) Assay Kit (Thermo Fisher Scientific, UK) using the Qubit™ fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration of the membrane fractions and whole cell lysates were measured using the Pierce BCA protein assay kit (Thermo Fisher Scientific, 23227). Myc-tag immunoprecipitation (IP ...
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bioRxiv - Microbiology 2024Quote: ... Quantification of isolated DNA was performed with the Qubit 4 fluorometer and the dsDNA HS Assay kit (Thermo Fisher Scientific, Waltham, MA, USA). DNA was sent to SeqCenter (Pittsburgh ...
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bioRxiv - Neuroscience 2024Quote: ... RNA samples were thawed on ice and concentrations were quantified using the Quant-iT™ RiboGreen™ RNA Assay Kit (ThermoFisher, Waltham, MA) and normalized to 10 ng/µL ...
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bioRxiv - Neuroscience 2024Quote: Total protein levels of all CSF samples were determined by use of a PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, USA). CSF Aβ40/Aβ42 and total tau (t-tau)/tau phosphorylated at threonine-181 (p-tau ...
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bioRxiv - Immunology 2024Quote: ... The final spike protein concentration was determined by measuring absorbance at 562 nm using Pierce™ BCA Protein Assay Kit (Thermo Scientific™). Proteins were dispensed into 0.5 ml aliquots and stored at -80°C.
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bioRxiv - Cell Biology 2024Quote: Hour 16 post-transfection control and SLC25A48-KO were incubated with 10 µM EdU (Click-iT Plus EdU Flow Cytometry Assay Kit, Thermo Fisher Scientific, USA) for 1 h in DMEM/F12 media ...
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Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: ... The number of double-stranded DNA in the genomic DNA samples was quantified by using the Qubit dsDNA HS Assay Kit (Invitrogen, cat no Q32851). Up to 2000 ng of double-stranded genomic DNA was fragmented by Covaris shearing to obtain fragment sizes of 160-180 bp ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were clarified by centrifugation for 15 min (21.000 x g and 4 °C) and protein content in the supernatant was determined using a Pierce™ BCA assay kit (Thermo Scientific, USA). Cell lysates were diluted to a final concentration of 2 mg protein/ ml in native lysis buffer and kept on ice until used freshly.
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bioRxiv - Genetics 2024Quote: ... 1:10 dilution of whole cell lysates in RIPA were used to quantify protein content using Pierce BCA Assay Kit (Thermo Fisher Scientific #23227). 20 μg protein was loaded per sample into 15-well Novex WedgeWell Tris-Glycine Mini gels (Invitrogen #XP08165BOX) ...
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bioRxiv - Genetics 2024Quote: ... 1:10 dilution of lysates in LP2 buffer were used to quantify protein content using Pierce BCA Assay Kit (Thermo Fisher Scientific #23227). The resulting 2′3′-cGAMP levels were normalized to protein content in each sample.
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bioRxiv - Microbiology 2024Quote: ... The quality and concentration of extracted DNA were determined using a Qubit 4 fluorometer with dsDNA broad range (BR) Assay kit (Thermo Fisher Scientific, Canada). DNA size distribution was confirmed by running a Pippin pulse electrophoresis (Sage science ...
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bioRxiv - Microbiology 2024Quote: ... xGen-prepared and SSLR-prepared libraries were measured by Qubit® 1X dsDNA HS Assay Kit on Qubit Fluorometer (Life Technologies, CA, USA) and fragment length distributions were determined using TapeStation 4200 (Agilent ...
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bioRxiv - Microbiology 2024Quote: ... DNA was eluted in 80 μl elution buffer and the concentration was measured using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, USA).
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the supernatant from each sample was then determined using the Pierce BCA protein assay kit (Thermo Scientific, Catalogue #23225) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We quantified the concentrations of double-stranded DNA using a Qubit® Fluorometer and the dsDNA BR (broad-range) Assay Kit (Thermo Fisher Scientific). We adjusted the DNA extracts to a final concentration of 50 ng/µl with nuclease-free water (Carl-Roth GmbH + Co ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed in Ripa Buffer and total protein was quantified using the Pierce™ BCA Protein Assay Kit (Thermo Scientific™, 23227) for data normalization.
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bioRxiv - Molecular Biology 2022Quote: ... two independent IPs were carried out using 120 μg of Arabidopsis chromatin mixed with 3 % of Drosophila chromatin quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). DNA samples eluted and purified from the two technical replicates were pooled before library preparation (Illumina TruSeq ChIP ...