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Citations for Thermo Fisher :
8601 - 8650 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... SuperScript III kit (Invitrogen) and random hexamer primers was used ...
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bioRxiv - Neuroscience 2023Quote: ... purified (MEGAclear Kit, Ambion), ethanol precipitated ...
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bioRxiv - Genetics 2023Quote: ... SYBR Green Kit (ThermoFisher) with the following parameters ...
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bioRxiv - Neuroscience 2023Quote: ... and Stain Kit (Affymetrix). Microarrays were scanned using a GeneChip Scanner 3000 (Affymetrix ...
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bioRxiv - Cell Biology 2024Quote: A kit by Invitrogen for Total RNA and protein isolation can be used ...
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bioRxiv - Molecular Biology 2024Quote: Assay Kit (Thermo Fisher) and an InfiniteⓇ M Plex microplate reader (Tecan).
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bioRxiv - Evolutionary Biology 2023Quote: ... Extractions were carried out with commercial extraction kits (Qiagen DNeasy plant kit, Venlo, Netherlands; ChargeSwitch gDNA plant kit, Invitrogen, Carlsbad, USA) following the manufacturer’s protocols or using the CTAB method (Doyle and Doyle ...
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bioRxiv - Neuroscience 2024Quote: ApoE and sAPP were measured in human CSF samples using the Apolipoprotein E Human ELISA Kit (#EHAPOE) and Amyloid Precursor Protein Human ELISA Kit (#KHB0051) kits respectively from Life Technologies, following the manufactureŕs protocol in duplicates using a 1:100 dilution of CSF.
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bioRxiv - Cancer Biology 2022Quote: ... mixed 1:1 with addition of 1% B27 (Invitrogen), 0.5% N2 (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... treated 1:1 with chloroform (Fisher Scientific, C606-1) for 15 minutes ...
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bioRxiv - Biophysics 2020Quote: ... The concentrations of cdM2-1 and RNA were determined by absorbance at 280 and 260 nm using extinction coefficients of 5,960 M−1·cm−1 (Expasy-ProtParam) (14) and 8,050 M−1·cm−1·base−1 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Total RNA (1 μg) from each sample was used for reverse transcription with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Life Technologies). Real-time PCR was performed in ABI Step One Plus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: SpG and SpRY mRNA were in vitro transcribed from DNA linearized by XbaI (1 μg) using the mMESSAGE mMACHINE™ T3 kit (Invitrogen, Thermo Fisher). In vitro transcribed mRNAs were DNAse treated using 1 μl TURBO-DNAse for 20 minutes at 37 °C and purified using RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Genomics 2020Quote: ... and subsequently subjected into nested polymerase chain reactions (PCR) according to manufacturer’s instructions prescribed in HIV-1 genotyping kit: Amplification module (Applied Biosystems, Life Technologies, Warrington, UK). The amplified cDNA was purified using ExoSAP-IT™ PCR product clean-up reagent (Applied Biosystems ...
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bioRxiv - Genomics 2019Quote: ... Reverse transcription reactions were performed on 1 µg of DNase-treated RNA using the RETROScript Reverse Transcription Kit (Ambion, AM1710, Thermo Fisher, Waltham, MA) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: Neonatal mouse ventricular myocytes (NMVMs) were isolated from 1-2 day old mice following Pierce™ primary isolation kit (88281, Thermo Fisher Scientific). After 72 hours in standard culture conditions ...
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bioRxiv - Neuroscience 2020Quote: ... 1 µg RNA and random hexamer primers were used for reverse transcription using the cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA, USA). RT-qPCR experiments were done with SYBR Green I in a Light Cycler 96® (Roche Applied Science ...
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bioRxiv - Bioengineering 2020Quote: ... cDNA samples were barcoded using Platinum PCR SuperMix High Fidelity polymerase and Ion Xpress RNA-Seq Barcode 1–16 Kit (Life Technologies, Carlsbad, CA). Yield and size distribution of the cDNA libraries were assessed using a DNA1000 chip (Agilent Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... After 24 hours the total RNA was extracted from each well and 1 μg of total RNA of primary microglial cells were reverse-transcribed using the High-Capacity cDNA Archive kit (Applied Biosystems, Carlsbad, USA), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... T4 phage were added (3×108 phages ml-1) to the AFM bio-cell together with SYTO 9 and PI (BacLight Bacterial Viability and Counting Kit, Invitrogen, ThermoFisher Scientific, United States) at concentration of 0.06 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... SIV gag RNA was quantified by qRT-PCR using the TaqMan RNA-to-Ct 1-Step Kit (Thermo Fisher Scientific, MA; Cat# 4392938) and Applied Biosystems QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mutant TDP-43 and 1 mutant C9ORF72 ALS patient were obtained by reprogramming fibroblasts (Additional file 1: Table S1) with CytoTune-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific, Waltham, MA) and differentiated into neurons ...
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bioRxiv - Cell Biology 2019Quote: ... ~1 μg purified RNA was used to generate cDNA via High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Cat. No. 43-688-13). qRT-PCR was performed with TaqMan Universal Master Mix II ...
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bioRxiv - Plant Biology 2020Quote: ... and 1 cycle of 85°C for 5 min) was performed using Revert Aid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, USA) with specific RT stem-loop primers and oligo (dT) ...
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bioRxiv - Microbiology 2019Quote: ... RNA levels were determined with Power SYBR® Green RNA-to-CT™ 1-Step Kit using a StepOne Plus Real-Time PCR system (Applied Biosystems). For relative quantification ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: The concentration of IL-1β in GAS muscle extracts at 2-day post-surgery was measured using Mouse IL-1 β ELISA Kit (Invitrogen #88-7013), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was purified from 1 μg RNA per sample using the commercially available Click-iT Nascent RNA Capture Kit (Life Technologies, Carlsbad, CA). cDNA was generated on 500 ng of total RNA and all EU affinity-purified RNA using the SuperScript VILO cDNA Synthesis Kit (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... All cells from each tissue were resuspended with the D10 buffer to a concentration of 50-500 million cells per milliliter and stained with Calcein AM (Component A: AM) and Ethidium homodimer-1 (Component B: EH) in LIVE/DEAD Viability/Cytotoxicity Kit (Invitrogen, Cat. no. L3224) for 20 min on ice ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA (1 μg) was reverse transcribed with random hexamers using Hight-Capacity cDNA Reverse Transcription Kit with Rnase Inhibitor (Applied Biosystems, ThermoFisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The qRT-PCR was performed in triplicate using 100 ng of RNA and the TaqMan RNA-to-CT 1-Step Kit (Applied Biosystems, Carlsbad, CA) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Relative RNA levels were determined by RT-qPCR using Power SYBR® Green RNA-to-CT™ 1-Step Kit (Thermo Fisher, # 4389986), StepOnePlus ™ Real-Time PCR System (Applied Biosystems TM) ...
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bioRxiv - Genomics 2022Quote: ... and FKBP5 were determined by qRT-PCR using the Power SYBR™ Green RNA-to-CT™ 1-Step Kit (Applied Biosystems Inc.). 100ng of total RNA was used for each reaction ...
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bioRxiv - Cancer Biology 2022Quote: Reverse transcription was performed on 1□µg of total RNA using a high-capacity cDNA reverse transcription kit (Thermo Fisher Scientific, Illkirch, France). cDNAs were amplified by PCR in a Roche real-time thermal cycler ...
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bioRxiv - Cell Biology 2022Quote: ... 100 ng RNA were used for real-time PCR with the Power SYBR® Green RNA-to-CT™ 1-Step kit (Applied Biosystems) and the CFX96 Touch Real-Time PCR Detection System (Bio-RAD ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA synthesis was performed using 1 µg of total RNA using a RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific™), following the manufacturer’s recommended protocol and the standard guidelines of reverse transcription ...
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bioRxiv - Developmental Biology 2020Quote: ... treated with DNase 1 to remove genomic DNA and reverse transcribed using the Maxima First Strand cDNA synthesis kit (ThermoFisher Scientific, Waltham, MA). Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of cellular total RNA was reverse transcribed to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Cat# 4368813). qPCR was then performed using a QuantStudio 6 Flex Real-Time PCR Systems (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: ... Then cDNA was synthesized from 1 μg of total RNA using the RevertAid First Strand cDNA Synthesis kit (Thermo Fisher Scientific, Cat. K1622), with random hexamer as the primer ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative reverse transcription PCR reactions used Power SYBR™ Green RNA-to CT™ 1-step kit (Applied Biosystems-Thermo Fisher Scientific, USA). Thermocycling began with a Hold Stage (first step at 48 °C for 30 s ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative reverse transcription PCR reactions used Power SYBR™ Green RNA-to CT™ 1-step kit (Applied Biosystems-Thermo Fisher Scientific, USA). Thermocycling began with a Hold Stage (first step at 48 °C for 30 s ...
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bioRxiv - Microbiology 2021Quote: ... Real-time RT-PCR was performed with the Power SYBR Green RNA-to-CT 1-Step Kit (Applied Biosystems, Foster City, CA, USA) using an AriaMx Real-Time PCR system (Agilent ...
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bioRxiv - Bioengineering 2021Quote: ... These were then treated with 10 µM Ethidium Homodimer-1 and 5 uM Calcein AM from a Live/Dead Test Kit (Molecular Probes, Thermofisher Scientific) to a total volume of 250 uL PBS and incubated (37°C ...
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bioRxiv - Physiology 2020Quote: ... RNA was measured using a Nanodrop 1 μg of RNA was then reverse transcribed into cDNA (Applied Biosystems, High Capacity cDNA kit, 4368813) with additional RNase inhibitor (Invitrogen RNase OUT ...
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bioRxiv - Physiology 2020Quote: ... Samples were homogenized and 1 µg of RNA was treated with DNaseI prior to cDNA synthesis using the High Capacity Reverse Transcription Kit (Applied Biosystems, Carlsbad, CA). A Reverse Transcriptase negative control was included to control for genomic DNA contamination ...
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bioRxiv - Cancer Biology 2021Quote: ... Real-time quantitative PCR (RT-qPCR) was then carried out using TaqMan™ RNA-to-CT™ 1-Step Kit (Thermo Fisher Scientific). PCR was performed using individual TaqMan Assays with catalogue numbers provided in supplementary table 1 with searchable Research Resource Identifier (RRID) ...
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bioRxiv - Plant Biology 2021Quote: ... the total RNA concentration was adjusted to 2.95 μg total RNA·μL−1 and treated with DNase (TURBO DNA-freeTM Kit, Ambion Inc., The RNA Company, USA) to remove residual DNA ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were incubated for 8 minutes in TSA Plus Fluorescein solution (1/50 TSA Plus Fluorescein in 1x Amplification Buffer (Tyramide Signal Amplification Labeling Kit No. 2; Molecular Probes, Thermo Fisher Scientific)) in darkness ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of RNA were reverse transcribed and amplified using the TaqMan™ RNA-to-CT™ 1-Step Kit (Applied Biosystems #4392938) on the ABI QuantStudio 7 Flex Real-Time PCR System ...
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bioRxiv - Microbiology 2021Quote: ... and cDNA and no-RT controls were synthesized from 200 ng to 1 ug RNA using the SuperScript VILO cDNA synthesis kit (Life Technologies, Carlsbad, CA). The qRT-PCR reactions were run in triplicate with 5 ng cDNA and Power Up SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2019Quote: ... naïve CD4+ CD25− T cells at density of 1×106/well were pre-stained with CellTrace™ CFSE Proliferation Kit (ThermoFisher Scientific, MA). After 24 h ...