Labshake search
Citations for Thermo Fisher :
8551 - 8600 of 10000+ citations for Fatty acid binding protein FABP1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: ... Research-grade DynabeadsTM Human T-Expander CD3/CD28 beads (Gibco) were washed using DynaMag-2 magnet (Gibco ...
-
bioRxiv - Cancer Biology 2024Quote: ... CAFs were kept in Human Plasma-Like Medium (HPLM, Gibco) with ascorbic acid for 11 days ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 0.2 Units/mL human recombinant insulin (Gibco, #12585-014), seeded on plates coated with rat tail collagen (BD Biosciences) ...
-
bioRxiv - Immunology 2024Quote: ... Dynabeads Human T-Activator CD3/CD28 (11161D; Gibco; 1:100) or plate bound with anti-CD3 and anti-CD28 antibodies (0.5µg/ml each ...
-
bioRxiv - Immunology 2024Quote: ... or anti-human IgG Alexa Fluor 647 conjugate (Invitrogen, A21445) or Streptavidin Alexa Fluor 647 conjugate (Invitrogen ...
-
bioRxiv - Physiology 2024Quote: Human islets were cultured in CMRL medium (Gibco 11530-037) supplemented with 10% FBS ...
-
bioRxiv - Cell Biology 2024Quote: ... and 5 ng/mL recombinant human EGF (Gibco Cat# PHG0311).(22 ...
-
bioRxiv - Cell Biology 2024Quote: ... siRNAs were purchased from Life Technologies (for human cell lines) and from Horizon Discovery (for human PCLS) ...
-
bioRxiv - Cancer Biology 2024Quote: ... recombinant human EGF (Thermo Fisher Scientific, #PHG0311, 50 ng/ml), N-acetylcysteine (Sigma Aldrich ...
-
bioRxiv - Microbiology 2020Quote: ... microbial protein fermentation was assessed by measuring the protein concentration using Thermo Scientific™ Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA) and the ammonia concentration using Sigma-Aldrich® Ammonia Assay Kit (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2021Quote: ... microbial protein fermentation was assessed by measuring the protein concentration using Thermo Scientific™ Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA) and the ammonia concentration using Sigma-Aldrich® Ammonia Assay Kit (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2022Quote: ... The protein concentration of the supernatant for all protein samples was determined by Pierce BCA Protein Assay Kit (#23227, Thermo Scientific, Rockford, IL, USA).
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... The protein concentration was determined using a BCA protein assay kit (Pierce® BCA Protein Assay Kit; 23225, Thermo Fisher Scientific Inc., Victoria, Australia). Gel electrophoresis was performed using Bolt 4–12% Bis-Tris Plus gels (Life Technologies ...
-
bioRxiv - Microbiology 2023Quote: ... Protein purification was monitored using 12% SDS-PAGE and protein concentration was determined with the Qubit™ protein assay (Thermo Fisher Scientific, catalog no.Q33211).
-
bioRxiv - Biophysics 2019Quote: ... The purity of affinity purified histidine tagged CsgF was investigated using SDS PAGE and the presence of the histidine tag was confirmed by western blot using an Anti-His (C-Term)-HRP antibody (Life Technologies, Carlsbad, CA).
-
bioRxiv - Microbiology 2021Quote: The production of His-tagged soluble ACE2 was performed in FreeStyle™ 293-F cells and purified using HisPur™ Ni-NTA Resin (Thermo Scientific) as described previously [31].
-
bioRxiv - Molecular Biology 2021Quote: ... enrichment and chip loading was performed on an Ion Proton Chef system using the Ion PI™ Hi-Q™ Chef Kits (ThermoFisher Scientific); sequencing was performed on an Ion Proton PI™ V3 chip ...
-
bioRxiv - Immunology 2022Quote: ... Human PBMCs and C57BL/6 mouse PBMCs not immediately used in any experiments were stored down by resuspending cells in freezing media (HI-FBS with 10% DMSO, Fisher Scientific, BP231-100) in aliquots of 5-10 x106 cells per ml and frozen down to -80°C at a speed of 1°C/min prior to storage in liquid nitrogen.
-
bioRxiv - Immunology 2022Quote: ... Quantitative real-time PCR was performed by using qPCRBIO Syber Green Mix Hi-ROX (PCR Biosystems, Wayne, US) and run with the StepOne Real-Time PCR System (Applied Biosystems, Waltham, US). The relative gene expression was calculated as 1/2ΔCT based on the normalization by the reference gene Hprt1 ...
-
bioRxiv - Cell Biology 2020Quote: ... purified His-calmodulin was dialyzed in a Spectra/Por™ 4 RC Dialysis Membrane Tubing (08-667D, Thermo Fisher Scientific, Waltham, MA, USA) against 1,000 volumes of PBS supplemented with 0.5 mM EGTA at 4 °C for 4 h and replaced with fresh 1,000 volumes of PBS twice.
-
bioRxiv - Cell Biology 2021Quote: ... The second step was the separation of mCherry-zyxin or EGFP-VASP from 6xHis-MBP and the removal of the His-tagged TEV protease by Ni-NTA chromatography (HisPur™ Ni-NTA Resin, Thermo Scientific: 88222). First ...
-
bioRxiv - Microbiology 2020Quote: ... and blots were incubated for 1 h with a 1:1,000 dilution of anti 6X-His Tag rabbit antibodies (Thermo Fisher Scientific, Massachusetts, USA) followed by a 1 h incubation with a 1:3,000 dilution of goat anti-rabbit IgG coupled to HRP secondary antibodies (Thermo Fisher Scientific) ...
-
bioRxiv - Biophysics 2022Quote: ... anti-His tag antibody molecules were tagged with the dye Alexa Fluor® 647 NHS Ester (Succinimidyl Ester, ThermoFisher SCIENTIFIC, Catalog number: A20006) using the same procedure as followed in our previous paper.(11 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
-
bioRxiv - Molecular Biology 2024Quote: ... were amplified from differentiated P19 mouse embryonal carcinoma cells15 and ligated into the multiple cloning site of the expression vector pcDNA3.1 myc-His B (Thermo Fisher Scientific; Waltham, MA). Snapin was cloned in-frame with the downstream myc-His tags ...
-
bioRxiv - Genetics 2024Quote: ... Membrane was stripped using PVDF stripping solution (DOT Scientific 65000-500) according to manufacturer instructions and reprobed with anti-6x-His primary antibody (Thermo Fisher MA1-21315) and conducted identically to the first probing ...
-
bioRxiv - Neuroscience 2023Quote: An PPP2R2B-AS1 fragment containing the region 44 bp upstream of the CTG repeat and 145 bp downstream from the repeat was PCR-amplified and cloned into pcDNA3.1(-) myc-his A vector (Life Technologies, Grand Island, NY) at EcoRV site ...
-
bioRxiv - Immunology 2023Quote: ... The resulting libraries were multiplexed and sequenced with Ion Proton technology and Ion Hi-Q chemistry (Ion Torrent, Thermo Fisher Scientific, Vienna, Austria). CUTADAPT 4.0 was used to trim sequencing reads of low-quality bases and poly-A tails with the options -q 20 ...
-
bioRxiv - Biochemistry 2023Quote: ... The membrane was probed with primary antibodies including anti-tau mouse ET3 (1:1000 dilution) and 6X - His Tag rabbit monoclonal antibody (Invitrogen, 1:3500 dilution). The secondary antibodies used were ...
-
bioRxiv - Cell Biology 2023Quote: Primary antibodies used in western blotting (WB) and immunofluorescence (IF) were anti-6x His mouse monoclonal (Invitrogen, #MA1-21315, 1:5000 in WB), anti-GST mouse monoclonal (Invitrogen ...
-
bioRxiv - Plant Biology 2024Quote: ... to digest the phosphorylated OsPrx20-His and analyzed by the LC-MS/MS system (LTQ Orbitrap XL ETD; ThermoFisher Scientific, Waltham, MA, USA) as described previously (Gampala et al. ...
-
bioRxiv - Microbiology 2020Quote: ... The total protein content was measured using the Pierce BCA Protein Assay kit (Thermo Fisher) and 7.5 µg of lysate was run on a 4-12% Bis-Tris sodium dodecyl sulfate (SDS)-polyacrylamide gel with MOPS running buffer (Invitrogen ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations were measured using BCA protein assay kit (ThermoFisher Scientific; Waltham, MA, United States) in accordance with manufacturer’s instructions and 10 μg of protein was loaded per lane (N = 3 - 4) ...
-
bioRxiv - Cancer Biology 2021Quote: One mg protein sample was precleared with Dynabeads Protein A (Thermo Fisher, UK, Cat.100002D) and 1 μg isotype IgG2a (Cell Signalling ...
-
bioRxiv - Cancer Biology 2021Quote: ... p53-associated protein were pulled down by 5 μl DynabeadsTM protein G beads (#10004D, Invitrogen) at 4 °C for 1-1.5 h ...
-
bioRxiv - Cancer Biology 2021Quote: ... and the protein concentration was determined by a Pierce BCA protein assay (Life Technologies, #23225). Equal amounts of protein were loaded into 8% Bolt™ Bis-Tris Plus gels (Life Technologies ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein levels were normalised across samples using a Pierce™ BCA protein assay kit (ThermoFisher) according to manufacturers instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... The supernatant was collected for protein concentration quantification with BCA Protein Assay Kit (ThermoFisher Scientific). An equal amount of protein from each sample was subjected to quantitative analysis of IL-8 and IL-1β using ELISA kit (R&D system ...
-
bioRxiv - Cell Biology 2020Quote: ... the proteins were separated with the NuPAGE system (ThermoFisher, 4-12% Bis-Tris protein gel), and transferred to PVDF membrane (ThermoFisher) ...
-
bioRxiv - Cell Biology 2020Quote: ... and the protein concentration was determined using the BCA Protein Assay Kit (#23227, Thermo Scientific).
-
bioRxiv - Cell Biology 2020Quote: ... Total protein concentration was measured using a Pierce BCA protein assay kit (Thermo Scientific Fisher). After boiling the samples in Laemmli buffer at 95°C for 5 min ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentration was estimated using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) according to manufacturer protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined with the Pierce 660nm Protein Assay (Thermo Fisher Scientific, cat# 2262). Proteins were resolved by standard SDS polyacrylamide gel electrophoresis using pre-stained PAGE RULER plus (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2021Quote: ... Equal amounts of protein were loaded in NuPAGE bis-tris protein gels (Thermo Fisher Scientific) and electroblotted to a polyvinylidene difluoride (PVDF ...
-
bioRxiv - Microbiology 2020Quote: ... The protein concentration was assessed using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific). The mitochondria were pelleted by centrifugation (14,000 x g ...
-
bioRxiv - Microbiology 2019Quote: ... The proteins concentration was determined using a BCA Protein Assay Reagent Kit (Thermo Fisher Scientific) and stored at −80°C.
-
bioRxiv - Genomics 2020Quote: ... 5 µg antibody was coupled to 50 µl Protein A or Protein G Dynabeads (Invitrogen). The following antibodies were used ...
-
bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was quantified using the BCA Protein Assay Kit (Fisher Scientific, Cat# 23227). Samples were normalized to a fixed amount of protein ...
-
bioRxiv - Biochemistry 2022Quote: ... Total protein content was estimated using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific).
-
bioRxiv - Neuroscience 2021Quote: ... and 0.01% bromophenol blue) and protein concentrations were measured using Pierce 660nm Protein Assay (ThermoFisher).