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Citations for Thermo Fisher :
8501 - 8550 of 10000+ citations for Recombinant Human GC Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Lysates were boiled and sonicated (15 cycles of 30s on/30s off) and protein concentrations determined by EZQ® Protein Quantitation Kit (Invitrogen). Lysates were then alkylated with iodoacetamide (IAA ...
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bioRxiv - Biophysics 2019Quote: ... the concentration of virus was estimated as we have reported previously (15) by measuring the viral protein concentration using a BCA protein concentration assay kit (ThermoFisher Scientific) and then dividing the measured protein concentration by the average total protein molecular weight per viral particle (1.8 × 108 Da ...
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bioRxiv - Cell Biology 2019Quote: ... The concentration of protein on beads and whole cell extracts were measured using the BCA assay (Pierce BCA protein assay kit, ThermoFisher Scientific).
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bioRxiv - Neuroscience 2019Quote: ... Afterwards the supernatant was transferred into a fresh tube and the protein concentration was determined using a BCA protein assay kit (Thermo Fisher scientific ...
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bioRxiv - Cell Biology 2019Quote: ... pH8.0) added of protease inhibitors and protein lysates were quantified by Pierce™ BCA Protein Assay Kit (#23225, Thermo Fisher Scientific). Lysates were separated on SDS-PAGE and wet-transferred to nitrocellulose membranes (#NBA083C ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentration was determined using Pierce™ BCA Protein Assay Kit according to the manufacture’s manual (ThermoFisher Scientific, Catalog number: 23227). 40µg of protein from each sample was loaded onto the 4– 15% Mini-PROTEAN® TGX™ Gel (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... The immune-reactive proteins were detected using the protein bands were visualized using SuperSignalTM West Dura Extended Duration Substrate (ThermoFisher Scientific) and visualized on Image Quant LAS 500 machine (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Lipid peroxidation levels were normalised against total protein quantitated using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, United States).
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bioRxiv - Genetics 2020Quote: ... Protein quantification of the supernatant was performed using the Pierce™ BCA protein assay kit (Thermo-Fisher Scientific, Rockford, IL, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... liver tissues were lysed in RIPA buffer and total protein concentrations were determined by MicroBCA protein assay kit (Thermo Scientific, 23235) according to the manufacturer’s instructions and infinite 200 PRO microplate reader (Tecan) ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were cleared by centrifugation and protein-containing supernatants were collected into fresh tubes and quantified with the Pierce BCA protein assay kit (Thermo Scientific) using a two-fold dilution curve of BSA (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Evaluation of protein expression was performed by running 20 μg of total protein lysate on NuPage Bis Tris 4%-12% gels (Invitrogen, USA). Proteins were transferred using iBlot dry transfer system (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... purified NHR2 protein was labeled either with the dye Alexa Fluor® 488 (NHS Ester Protein Labeling kit, ThermoFisher Scientific, USA) or Monolith NT™ (Protein Labeling Kit Blue-NHS ...
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bioRxiv - Bioengineering 2021Quote: ... The supernatants were transferred into new tubes and protein concentration was measured using the Pierce 660 nm Protein Assay (ThermoFisher Scientific). Proteins were resolved on 4 – 20% SDS-PAGE gels (BioRad) ...
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bioRxiv - Bioengineering 2020Quote: Total adsorbed protein in a defined volume was determined by measuring protein concentration with the Pierce 660nm assay (ThermoFisher Scientific, Inc.) calibrated against a bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2021Quote: ... Pellets were resuspended in PBS and semEV concentrations were estimated by BSA protein kit (Thermo Fisher, Pierce BCA Protein Assay Kit) and adjusted to 2mg/mL ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cortical lysates were prepared according to the assay type and protein concentration was determined using BCA Protein Assay Kit (ThermoFisher Scientific) prior to the assays ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were centrifuged at 4 °C for 10 minutes at 16000 g to remove cell debris and protein concentration of the lysate was measured using Pierce BCA Protein Assay Kit (Thermo Scientific). Equal amounts (10 µg ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in Radioimmunoprecipitation assay (RIPA) buffer and protein concentration was determined by using Micro BCA TM protein assay kit (Thermo Fisher). Western blot was performed as described previously (32) ...
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bioRxiv - Cell Biology 2021Quote: Equal amounts or fractions of indicated protein lysates were loaded onto NuPage 4-12% Bis-Tris Protein Gels (Invitrogen, Waltham, MA), and then subsequently immunoblotted with the primary antibodies listed in Supplementary Table 2 ...
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bioRxiv - Physiology 2020Quote: ... 8 mg of the soluble protein fractions were pre-cleared with 200 μL of protein G-magnetic Dynabeads (Thermo Fisher Scientific) for 1 hr ...
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bioRxiv - Biochemistry 2020Quote: A 5-mL HiTrap™ Protein G HP column (Cytiva) was equilibrated with 10 CVs of Protein G IgG Binding Buffer (pH 5.0; Thermo Fisher Scientific). Spent medium containing CR3022 mAb was diluted with binding buffer at a 1:1 volume ratio and filtered using a 0.22 μm stericup ...
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bioRxiv - Cell Biology 2021Quote: ... Total protein concentrations of the supernatants were determined using a Pierce BCA protein assay kit (Thermo Scientific, catalog # 23225, lot # SJ256254) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Segregation and enrichment of proteins from different cellular compartments were performed using a Thermo Scientific Subcellular Protein Fractionation Kit (78840, Thermo Fisher) followed the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... A small volume of each sample was used to immediately measure protein concentration with the Pierce BCA Protein Assay kit (ThermoFisher; 23227). The rest was combined with 6X loading dye (375mM Tris pH 6.8 ...
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bioRxiv - Microbiology 2020Quote: ... Samples were homogenised using a VDI 12 tissue homogeniser and protein was quantified using a bicinchoninic acid (BCA) Protein Assay Kit (Thermo Scientific). 100 μg of total lung protein were lysed in 2x sample buffer (4% [w/v] SDS ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then resolved on 4-12% Bis-Tris gels (for lower molecular weight proteins) or 3-8% Tris-Acetate gels (for higher molecular weight proteins) (NuPage, Thermo-Fisher Scientific) and run in MOPS or Tris-Acetate running buffer respectively ...
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bioRxiv - Systems Biology 2021Quote: ... Lysates were cleared by centrifugation at 16,000 x g for 10 minutes at 4°C and protein concentration was determined using the BCA protein assay kit (Thermo Scientific). Fifty micrograms of protein was reduced with 5 mM Tris 2-carboxyethylphosphine (TCEP ...
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bioRxiv - Microbiology 2021Quote: ... A portion (5 µl) was kept to quantify the total amount of proteins using Pierce™ BCA protein assay kit (23225, ThermoFisher). 5 ml of HNO3 5% (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... 10uL of the protein lysate was loaded into a bis-tris protein gel (4-12% Invitrogen Bolt Bis-Tris mini gel), ran at 150V for 35min then transferred to PVDF membrane using the iBlot2 system ...
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bioRxiv - Microbiology 2021Quote: ... Eluted protein was buffer exchanged with the same elution buffer but without imidazole using 30 kDa MWCO protein concentrator (ThermoFisher Scientific). Final protein concentration was measured by spectrophotometry.
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bioRxiv - Microbiology 2020Quote: ... as measured on protein level (BCA protein concentration determination kit; before digestion) was labeled with 10-plexing tandem mass tags (TMT-10; Thermo Scientific) following the manufactures instructions ...
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bioRxiv - Microbiology 2020Quote: ... Enriched virus was inactivated with 2% sodium dodecyl sulfate (SDS) and protein concentration determined with a Pierce BCA protein assay kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Conditional supernatants from lentivirus-infected CHO cells sorted by FACS were used to purify the FXa-Fc fusion protein using a protein G column (89927, Thermo Fisher). For in vivo testing ...
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bioRxiv - Plant Biology 2021Quote: ... Protein concentration of the extracts was estimated based on tissue weight or normalized using Pierce Coomassie Protein Assay Kit (ThermoFisher Scientific). Co-immunoprecipitation (coIP ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations of all tissue fractions and TL were determined using the Bicinchonic acid assay (BCA Protein Assay Kit, Thermo Scientific). Bovine serum albumin was used as a protein standard ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein concentration was determined using the Bio-Rad Bradford protein assay kit according to the manufacturer’s instructions (ThermoFisher Scientific, #23225). An adapted BODIPY (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... frozen EVs were resuspended in RLT lysis buffer and RNAse-free water in a ratio of 1:3 and loaded based on protein concentration measured by Micro BCA Protein Assay Kit (Thermo Scientific). 2,8 μg of proteins were loaded.
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bioRxiv - Neuroscience 2021Quote: ... Protein samples (10 μg/well) were loaded to NuPAGE Novex 4–12% Bis-Tris protein gel (ThermoFisher Scientific, Carlsbad, CA, NP0336BOX) and transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2021Quote: Cells were lysed in Radioimmunoprecipitation assay (RIPA) buffer and protein concentration was determined by using Micro BCA TM protein assay kit (Thermo Fisher). Protein extracts were loaded onto SDS poly-acrylamide gels and run in 1x Tris-glycine SDS buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were cleared by centrifugation and protein concentrations were determined using the Pierce 660 nm Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of protein were diluted in Laemmli sample buffer ...
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bioRxiv - Microbiology 2021Quote: ... the purified proteins were loaded on an SDS-PAGE and silver stained for protein visualization (Pierce Silver Stain Kit, Thermo Scientific). Coomassie stained SDS-PAGE samples were sent for mass spectrometry analysis.
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bioRxiv - Cell Biology 2021Quote: ... Lysates were clarified by centrifugation (20000 g, 10 minutes, 4 °C) and protein concentrations were determined using the BCA protein assay kit (ThermoFisher Scientific). The same amount of protein (1.2 μg ...
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bioRxiv - Cell Biology 2021Quote: ... The concentration of protein per sample was determined using the Bicinchoninic Acid (BCA) Protein Assay Kit (23228, Pierce/Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... alongside Chameleon Duo Protein Ladder (3 μL/lane; LiCOR, 928-60000) or PageRuler Protein Ladder (5 μL/lane; Thermo Scientific, 26616) and run in pre-chilled 1X MOPS Buffer (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were pelleted for 15 min at 13 000 xg at 4 °C and supernatants kept for protein quantification (BCA Protein Assay Kit, Thermo Scientific). Equal amounts of cellular proteins were resolved on 10% or 12% sodium dodecyl sulfate – polyacrylamide gels (SDS-PAGE ...
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bioRxiv - Cell Biology 2021Quote: ... POS preparations were washed in PBS and resuspended in medium and protein content was measured (BCA Protein Assay Kit, Thermo Scientific) and in parallel ...
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bioRxiv - Cell Biology 2021Quote: ... The final pellet was resuspended in 200 μL of extraction buffer and the mitochondrial protein concentration was determined with the Pierce BCA Protein Assay Kit (Thermo Scientific). For the Proteinase K accessibility assay ...
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bioRxiv - Neuroscience 2022Quote: ... and human brain tissues were prepared using a RIPA buffer and the protein contents were measured using a PierceTM BCA Protein Assay Kit (Thermo Scientific). Proteins were separated on 12% SDS-PAGE gradient gel and transferred onto a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... Cellular debris was pelleted for 15 minutes at 4 °C and protein concentration was quantitated with the Coomassie (Bradford) Protein Assay (ThermoFisher Scientific). A total of 15μg (organoids ...