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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for tert Butyl 3 5 2 amino ethyl thiophen 3 yl propionate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... 45 mM MgCl2) and 500 μM dithiobis(succinimidyl propionate) (DSP, ThermoFisher). All buffers used in the nuclei isolation and sorting contained 0.4 U/mL RNAse inhibitor murine (New England Biolabs) ...
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bioRxiv - Cell Biology 2020Quote: ... and treated with 0.5mM dithiobis(succinimidyl propionate) (DSP) (Thermo Scientific, PG82081) in PBS for 30 minutes at room temperature while rotating ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were incubated with 1 mM dithiobis(succinimidyl propionate) (DSP; ThermoFisher) at 25°C for 1 h and quenched by the addition of 40 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Cell Biology 2024Quote: ... then incubated in 1 mM dithiobis(succinimidyl propionate) (DSP, ThermoFisher #22585) on ice for 1-hour to covalently crosslink interacting proteins ...
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bioRxiv - Cell Biology 2024Quote: ... then incubated in 1 mM dithiobis(succinimidyl propionate) (DSP, ThermoFisher #22585) on ice for 1-hour to covalently crosslink interacting proteins ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Bioengineering 2021Quote: ... The reaction was monitored using an Agilent 1100 HPLC system equipped with a MAbPac HIC-Butyl column (4.6 × 100 mm, 5 µm, Thermo Scientific). Elution conditions were as follows ...
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bioRxiv - Bioengineering 2020Quote: ... was analyzed using an Agilent 1100 HPLC system equipped with a MAbPac HIC-Butyl column (4.6 × 100 mm, 5 µm, Thermo Scientific). Elution conditions were as follows ...
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bioRxiv - Biochemistry 2020Quote: ... and SARS-CoV-2 [3]) was performed with Proteome Discoverer version 2.4 (Thermo Fisher Scientific). The search was restricted to human and SARS-COV-2 proteins ...
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bioRxiv - Cell Biology 2021Quote: ... 3 μg of RNA was digested with Turbo DNase (2 U/μl, # AM1907, ThermoFisher Scientific). 500 ng of DNase-digested RNA was tagged with G/I tails in a 10 μl reaction ...
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bioRxiv - Immunology 2022Quote: ... Cells were activated for 3 days in presence of 100 U/mL IL-2 (Gibco) and 1 µg/mL PHA (Remel).
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bioRxiv - Cell Biology 2021Quote: ... washed 3 times and incubated with 4’,6-Diamidino-2-phenylindole (DAPI; 2mg/ml) (Invitrogen) in PBS for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... or with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) for 30 min at room temperature (1:150 in AnnexinV binding buffer-Biolegend) ...
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bioRxiv - Microbiology 2021Quote: ... and 2-3 cm) after measuring the DNA concentration with Qubit (QubitⓇ 2.0 Fluorometer, Invitrogen). We obtained eight metagenomes from Lake Sarnen (deepest and shallow_inflow station for March ...
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bioRxiv - Evolutionary Biology 2022Quote: ... where an Acclaim PepMap 100 (2 cm x 75 μm, 3 μm particles; Thermo Fisher) pre-column was connected in front of an EASY-Spray PepMap RSLC C18 reversed phase column (50 cm x 75 μm ...
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bioRxiv - Cell Biology 2020Quote: ... After washing and nuclear counterstaining with 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher, 3 µM), sections were mounted on microscopic slides using Aqua Poly/Mount (Polysciences) ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were prepared by incubating with a 2% solution of 3-aminopropyltrimethyoxysilane (313255000, Acros Organics) diluted in isopropanol ...
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bioRxiv - Biochemistry 2021Quote: ... a pEntryslot3 vector containing the let-858 or tbb-2 3’UTR and pDEST (ThermoFisher) in an LR reaction ...
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bioRxiv - Cell Biology 2021Quote: siRNA transfections were performed 2 or 3 days before each experiment using Lipofectamine RNAiMax (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... All cell lines were passaged every 2-3 days using 0.05% Trypsin/EDTA solution (Gibco).
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were prepared by incubating with a 2% solution of 3-aminopropyltrimethyoxysilane (313255000, Acros Organics) diluted in isopropanol ...
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bioRxiv - Genomics 2022Quote: ... The remaining RNA (< 3 µg) was treated with 2 U TURBO™ DNase (Invitrogen, USA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Samples were DNase treated (3 µL of Turbo RNase-free DNase, 2 U/µL, ThermoFisher) for 20 min at 37°C and then precipitated by addition of 290 µL of water ...
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bioRxiv - Genomics 2024Quote: ... Cells were passaged every 2-3 days by detachment with 0.05% trypsin-EDTA (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... passage 2-3 NPCs were detached with Stempro Accutase cell dissociation reagent (A1110501, Life Technologies) and kept in cold PBS 1%FBS for the duration of the protocol ...
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bioRxiv - Immunology 2024Quote: ... 10% heat inactivated fetal bovine serum (HIFBS) 2 × 10-3 m L-glutamine (Life Technologies), antibiotic antimycotic (1× ...
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bioRxiv - Cancer Biology 2024Quote: Organoid images were digitally acquired every 2–3 days on an EVOS M7000 (ThermoFisher, AMF7000) with DiamondScope software (version 2.0.2094.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... The lungs were inflated with 1-3 ml 2% UltraPure Low Melting Point Agarose (Invitrogen). Lungs and livers were fixed overnight at 4°C on a shaker ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were aspirated and 2-3 crystals of Dil stain (Life Technologies-Molecular Probes) were added to each culture well and incubated on a shaker for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were aspirated and 2-3 crystals of Dil stain (Life Technologies-Molecular Probes) were added to each culture well and incubated on a shaker for 10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was carefully removed and resuspended in 2-3 mL of TrypLE Express (Gibco # 12605010) for 20 min at 37 °C ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were subcultured every 2-3 days and transfected with lipofectamine 2000 transfection reagent (Invitrogen) in Opti-MEM medium (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 ng equivalent was synthesized using Super Script 3 Supermix (18080400, Thermo Fisher, USA) with stem-loop primers and sly actin_R primers as internal controls (Table S2) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were passaged every 2–3 days using 0.25% Trypsin-EDTA (1X) (Gibco, Cat# 25200056) as the dissociation buffer ...
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bioRxiv - Genomics 2023Quote: ... mESCs were routinely passaged at ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher). Before experiments ...
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bioRxiv - Developmental Biology 2024Quote: ... Passaging was performed every 2 to 3 days using Accutase (00-4555-56, ThermoFisher Scientific). The cells were Mycoplasma tested prior to running experiments ...
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bioRxiv - Immunology 2024Quote: ... 10% heat inactivated fetal bovine serum (HIFBS) 2 × 10−3 m L-glutamine (Life Technologies), antibiotic antimycotic (1× ...
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bioRxiv - Zoology 2024Quote: ... He-pdf-2 and He-pdf-3 were ligated into pJET1.2 vector (Thermo Fisher Scientific) as per manufacturer’s specification and transferred to competent E ...
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bioRxiv - Developmental Biology 2024Quote: ... Cell passaging occurred every 2–3 days using 0.25% Trypsin-EDTA (1X) (Gibco, Cat# 25200056) as the dissociation buffer.
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bioRxiv - Developmental Biology 2024Quote: ... while the rest were re-plated onto three matrigel-coated 10 cm dishes (3 replicates / 3 million cells per plate) in N2/B27 media containing 3 µg/mL puromycin (Fisher Scientific; Cat. no. 501532829) to select for the pMK1334 sgRNA vector ...
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bioRxiv - Neuroscience 2023Quote: ... 5 mL MEM Non-essential Amino Acids (Thermo Fisher Scientific), 1 mL 200 mM ascorbic acid (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 ml 100x non-essential amino acids (Gibco #11140-35), 1 mL 500x β-mercaptoethanol (5mM ...
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bioRxiv - Immunology 2024Quote: ... 5 mM MEM 140 Non-Essential Amino Acids (NEAA; Gibco), 2mM Glutamax (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Intracellular NO production was assessed during reoxygenation by live-cell staining with the NO-sensitive fluorescent dye 4-Amino-5-Methylamino-2’,7’-Difluorofluorescein (DAF-FM) diacetate (Invitrogen). iPSC-ECs were stained with 1 μM DAF-FM diacetate for 10 minutes at 37°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The caspase-3 activity was quantified using EnzChek™ Caspase-3 Assay Kit #1 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: RNA was 3’ labelled with biotin using Pierce™ RNA 3’ End Biotinylation Kit (ThermoFisher) and purified using Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Bioengineering 2024Quote: ... Red blood cells were lysed following 3 × 3 min incubations with ACK Lysing Buffer (Gibco). The remaining white blood cells were washed with PBS twice and plated for surface staining ...
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bioRxiv - Microbiology 2023Quote: ... Head kidney pieces (3 mm × 3 mm) were collected in RNA later (Thermo Fisher Scientific), transferred to 500 μL MagNA Pure LC RNA Isolation Tissue Lysis buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Gdf1/3 and Gdf1/3-like mRNA were synthesized using T7 mMESSAGE mMACHINE kit (Ambion). Unfertilized eggs were injected with mRNAs and fertilized as previously described50 ...
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bioRxiv - Bioengineering 2024Quote: ... Dil Stain (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate (‘DiI’; DiIC18(3))) were purchased from ThermoFisher Scientific ...