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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for hsa mir 483 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... OsIRT2 and OsLCT1 expressed in leaf were analyzed by using Real-time RT-PCR (the primer sequences used for qRT-PCR are shown in Table 1., Synthesis by Invitrogen, Beijing). The total RNA samples were isolated by TRIzol reagent (TIANGEN BIOTECH ...
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bioRxiv - Molecular Biology 2020Quote: ... Real time analyses by two-step RT-PCR was performed for quantification of miRNA on a 7500 REAL TIME PCR SYSTEM (Applied Biosystems) or Bio-Rad CFX96TM real time system using Applied Biosystems Taqman chemistry based miRNA assay system.
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bioRxiv - Molecular Biology 2020Quote: Real time analyses by two-step RT-PCR was performed for quantification of miRNA and mRNA levels on a 7500 REAL TIME PCR SYSTEM (Applied Biosystems) or Bio-Rad CFX96™ real time system using Applied Biosystems Taqman chemistry based miRNA assay system ...
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bioRxiv - Immunology 2020Quote: ... and 5’-AATGGGAAGTCACGAAGGTG-3’(reverse) and quantitative real-time RT–PCR was conducted on a StepOne Plus PCR instrument (Applied Biosystems). The NF-κB-luciferase (Luc2p ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative real-time RT-PCR (qRT-PCR) was performed using a Fast SYBR Green Master Mix using the OneStep instrument (Applied Biosystems). The obtained values were normalized to either HPRT1 or GAPDH genes ...
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bioRxiv - Neuroscience 2022Quote: ... were loaded in technical quadruplicates for q-RT-PCR on a QuantStudio™ 6 Flex Real-Time PCR System (ThermoFisher, 4485691). The ΔΔCT method was used to provide gene expression values after normalising to the known reference gene B2M ...
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bioRxiv - Neuroscience 2022Quote: ... were loaded in technical quadruplicates for q-RT-PCR on a QuantStudio™ 6 Flex Real-Time PCR System (ThermoFisher, 4485691). The ΔΔCT method was used to provide gene expression values after normalising to the known reference gene B2M ...
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bioRxiv - Neuroscience 2023Quote: ... The expression of specific mRNAs was assayed using fluorescence-based quantitative real-time PCR (RT-qPCR) (Fast-SYBR PCR Master Mix; Applied Biosystems). Quantification reactions were performed in Duplicate for each sample using the ‘delta-delta Ct’ method ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time PCR was performed in MicroAmp optical 384-well plates using the QuantStudio 6 Flex RT-PCR system (Thermo Fisher). The default thermocycling program was used (95ºC for 10 min ...
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bioRxiv - Immunology 2023Quote: ... Quantitative RT-PCR was performed using Universal SYBR Green Supermix with StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA). Data was graphed as a 2ΔΔ CT fold change with the gene expression data being normalized to the house keeping gene RPS18.
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bioRxiv - Cell Biology 2021Quote: ... PLGA nanoparticles incorporating a mirVana miRNA mimic hsa-mir 132-3p (4464066, Thermo Fisher Science) were prepared using an emulsion solvent diffusion method in RNAase-free water ...
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bioRxiv - Molecular Biology 2021Quote: Synthetic full length hsa-miR-5010 intron RNA was generated by in vitro transcription with MEGAscript® T7 Kit (Invitrogen) using full length intron PCR amplification with a T7 encoding forward primer (Sup Table 1) ...
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bioRxiv - Molecular Biology 2020Quote: ... Real time PCR was performed on a 7500 fast Real-Time qPCR system (Applied Biosystems) using Fast SYBR Green mix (Applied Biosystems) ...
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bioRxiv - Bioengineering 2020Quote: ... Relative quantitation real-time qPCR was performed with StepOnePlus Real-time PCR system (Applied Biosystems) using Power SYBR Green PCR master mix (Applied Biosystems) ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative real-time reactions were performed on a 6500 Real-Time PCR System (Applied Biosystems) using ChamQ SYBR qPCR Master mix (Vazyme) ...
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bioRxiv - Developmental Biology 2022Quote: ... Real-time quantitative PCR was performed on a StepOnePlus real-time PCT Machine (Applied Biosystems). Taqman Universal PCR Master Mix (Thermo Fisher ...
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bioRxiv - Plant Biology 2020Quote: ... and subjected to the real-time qPCR using StepOnePlus Real-Time PCR system (Applied Biosystems) with KAPA SYBR FAST qPCR master mix (Kapa Biosystems) ...
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bioRxiv - Genomics 2022Quote: ... Real-time PCR was performed on a QuantStudio 6 Flex real-time thermocycler (Applied Biosystems) with the following thermocycling conditions ...
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bioRxiv - Bioengineering 2022Quote: ... Real time qPCR was performed using the QuantStudio 5 Real-Time PCR system (Applied Biosystems) using a total reaction volume of 20 μL ...
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bioRxiv - Cancer Biology 2023Quote: ... Real time quantification was performed in triplicate with ViiA Real-Time PCR system (ThermoFisher Scientific). Each sample was analyzed in triplicates ...
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bioRxiv - Zoology 2022Quote: ... Real-time PCR was performed with a 7300 Real Time PCR System using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). PCR reactions were run in triplicate using 1 μl of cDNA per reaction in a 20 μl volume ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed on a QuantStudio 12K Flex real-time PCR system (Applied Biosystems, Foster City, CA) by using Power SYBR Green Master Mix (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: Quantitative Real-time PCR (qRT-PCR) was carried out on a 7300 Real Time PCR system (Applied Biosystems, Carlsbad, CA, United States) to validate the transcriptome data ...
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bioRxiv - Microbiology 2019Quote: ... Expression profiles of the target genes were quantified by quantitative real-time PCR (qRT-PCR) in a QuantStudio 5 Real-Time PCR System (Applied Biosystems, USA). Each 20 μl reaction contained 1 μl of 2-fold diluted cDNA template ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed on a QuantStudio 12K Flex real-time PCR system (Applied Biosystems, Foster City, CA) by using Power SYBR Green Master Mix (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... the first round PCR products were diluted 10-fold and a total of 6.4 µL of the diluted PCR products were used for real-time PCR on the QuantStudio 3 Real-Time PCR Systems (Thermo Fisher Scientific) using the Perfecta qPCR ToughMix (QuantaBio) ...
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bioRxiv - Molecular Biology 2024Quote: ... The expression of the indicated target genes was determined by real-time PCR using the TaqMan® Fast universal PCR master mix and 7500 Fast Real-Time PCR System from Applied Biosystems. Based on Ct values ...
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bioRxiv - Neuroscience 2024Quote: ... were further evaluated for the quantitative real-time PCR (qRT-PCR) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative quantification of the long TMEM106B 3’ UTR and total TMEM106B levels was determined using the ΔΔCt method and normalized to two endogenous controls ...
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bioRxiv - Microbiology 2019Quote: ... with a Piko-Real Real-Time PCR System (Thermo Fisher Scientific) and diluted to 4 nM concentration ...
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bioRxiv - Cancer Biology 2022Quote: ... MicroRNA was firstly transcribed by miRCURY LNA RT kit to obtain its cDNA and examined for the expression level by specific primers designed for miR-4776-5p (Applied Biosystems). The quantitative PCR was performed by the miRCURY LNA SYBR Green PCR KiT (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: in vitro overexpression and knockdown of miR-654-5p were performed by Lipofectamine™ 3000 (Invitrogen)-mediated miRNA Agomir and Antagomir transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 nM of duplex miR-21-5p mimics (Thermo Fisher Cat#4464066 ID#MC10206 Lot# AS02E016), 100 nM of miR-21-5p inhibitor (Thermo Fisher Cat#4464084 ID#MH10206 Lot#AS02DR3S ...
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bioRxiv - Genetics 2019Quote: ... miR-199b-5p inhibitor or scrambled inhibitor negative control using Lipofectamine RNAiMAX (Life Technologies, Carlsbad, CA). Forty-eight hours after transfection ...
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bioRxiv - Cell Biology 2021Quote: HPK at 70% confluency were transfected with miRNA mimics at 10nM: miR-30a-5p (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... miR-125b-5p (MIMAT0000423; 50nM [28]) or control mimic (50nM; Life Technologies, USA, Supplementary Table 2), were delivered to primary luminal epithelial cells collected from gilts on 11-12 day of the estrous cycle ...
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bioRxiv - Developmental Biology 2023Quote: miR-139-5p expression levels were analyzed using TaqMan MicroRNA Assay (Cat#: 4427975, Thermo Fisher Scientific) and a TaqMan Universal Master Mix II no UNG with a StepOnePlus RT PCR system (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... The probe sequences for mmu-miR-129-5p and U6 snRNA were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Real-time RT-PCR was performed with the Power SYBR Green RNA-to-CT 1-Step Kit (Applied Biosystems, Foster City, CA, USA) using an AriaMx Real-Time PCR system (Agilent ...
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bioRxiv - Cancer Biology 2021Quote: ... Real-time quantitative PCR (RT-qPCR) was then carried out using TaqMan™ RNA-to-CT™ 1-Step Kit (Thermo Fisher Scientific). PCR was performed using individual TaqMan Assays with catalogue numbers provided in supplementary table 1 with searchable Research Resource Identifier (RRID) ...
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G protein-coupled Receptor Contributions to Wing Growth and Morphogenesis in Drosophila melanogasterbioRxiv - Developmental Biology 2022Quote: ... RT-qPCR reactions were carried out using the Quanta Biosciences™ one-step SYBR Green RT-qPCR kit (catalog #95087) and the Applied Biosystems StepOne™ Real-Time PCR System (ThermoFisher Scientific). Reactions were carried out in triplicate.
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bioRxiv - Cancer Biology 2021Quote: ... and subsequently used for qPCR analyses on a Step One Plus real time PCR detection system (Applied Biosystems). Expression levels of genes of interest were normalized to hypoxanthine guanine phosphoribosyl transferase (Hprt ...
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bioRxiv - Microbiology 2019Quote: ... Fungal biomass was then quantified by Real-Time PCR with an ABIPRISM 7000 Sequence Detection System (Applied Biosystems) using the Power SYBR Green PCR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Amplification and detection of the vRNA was performed in a StepOne Plus real-time PCR machine (Applied Biosystems). The cycling conditions were as following ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time PCR was performed in an ABI Prism 7700 sequence detection system (Applied Biosystems, Foster City, CA) using Syber Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Genomics 2023Quote: ... Amplification and detection of fluorescence were carried out on a QuantStudio3 real-time PCR system (Thermo Fisher Scientific) using the genotyping assay mode ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR detection was performed using PowerUp™ SYBR® Green Master Mix (Applied Biosystems, A25742) and StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2022Quote: ... The software used to analyze each RT-qPCR experiment was QuantStudio™ Real-time PCR Software (Applied Biosystems). Standard curves ...
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bioRxiv - Biochemistry 2021Quote: ... RT-qPCR reactions were run in duplicate on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems) with PowerTrack SYBR Green Master Mix (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR reactions were run in triplicate using an ABI 7500 Fast Real-Time PCR System (Applied Biosystems) and Apex qPCR 2X Master Mix Green ...
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bioRxiv - Microbiology 2021Quote: Quantitative RT-PCR was carried out with 7500 Fast Real Time Systems (Applied Biosystem, ThermoFisher Scientific, Massachusetts, USA). Each PCR reaction was conducted on a total volume of 10 µl ...